Rh4BG381200

SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015044
Physical Location & Seq
Forward (+)
53681183 .. 53683272
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG381200.1

Sequence Viewer

Length: 1617 bp
ATGAGAGAAACCCAACAACAGCTAGAAATGGCAGAAGAGCTAATGCAGCAGCTCAGATCCAAAGCCACAGAGCTCTTACTCAGAGAAGAATGGAGCGAATCCATACAAGTCTATTCTCAGTTCATCAATCTCTGCCAACACCACATCAATGATTATGAAACCACCCCAGACAACCTCCCAAAGCTCCACAAGTCCCTCTGCCTGGCCCTATCGAACCGAGCCGAAGCGCGATCCAGACTCCGAGACTATGCACAAGCCTTGAAGGATTGTGATGAGGCACTGAAAATTGAGAGCACCCATTTCAAGACTCTCATCTGCAAAGGTAAGATTTTGCTGAATTTGAACAGGTACTCCATGGCATTGACTTGCTTCAGAACTGCTCAGCTTGATCCTCAGGCAAATGGGAGCTCGGTTGGGCTTAATGGGTACCTGCAGAAATGCAAGAAGCTCGAACTGATGTCGAAAACTGGAGCTTTCGATATGTCTGAGTGGGTGATCAATGGGTTTCAGGCTAAGCCTCCGGAGCCGGCTGAGTACATTGGAGGGGCAGTGGAGATGAGGAAGTCGGGGATTAGAGGGCGTGGTTTGTTTGCGACGAAGAATATGGAAGCTGGGAGTTTGGTGCTGATTAATAAAGCTGTTGCAACGGAGAGAGGTATTTTGCCGGGTCAAGATTTGGATGAGAATGCCCAATTAGTTATGTGGAAGACTTTTACTGAGAAAGTGATGGATTCCGTGGCCAAATGTTCGAGAACATGTGGTTTGATTAGTACTCTGTCGAGTGGTGAAGATGAGGATGGGCTTGAGGTGCCTGAGATTAATCTGTTTAAGCCTGACTCGGAGCACTTTGGGTACTCAAATGAGAAGAAGCTTGATGCGAGTAGGGTTTTAAGTATTTTGGATGTCAATTCTATTGTTGAGGATTCCATTTCGTCTAAGGTTTTGGGGAAGAACAGTGATTACTATGGTGTTGGGCTTTGGGTACTTGCTTCATTCATCAACCATTCATGTATTCCCAATGCAAGGCGTTTGCATGTTGGGGACTATGTGATTGTTCATGCTTCAAGAGATGTCAAGGCTGGTGAGGAGCTAACATTTGCATATTTCGATGTGCTGAATCCGTTGGAAAAGCGCAACGAAATGTCAAAGACTTGGGGGTTTAGGTGCAACTGCAGGAGGTGTAAGTTTGAGGCTGAACTATGTTCGAGGCAAGAAGGCATTGGAGAGATTGAGATGGGTTTCGAAAGAGGGATGGATTCTGGTGCTGCGGTCTATAGGCTCGAGGAAGGCATGAGAAAATGGAAGGTGAAGGAAAAGGAAAAAGGGTACTTGAGAGCTTCATTTTGGGCTGCATATGGCTCAGAGAAGTTGATGAGAAGTTGGGGAAGACGGATCCCAATGATGGAGTCCGTGGTTGATAGCATTGTGGAAGCTGTGGGAAGTGATGAGAGGGTGTTGAAGATGGTGGTGGAGAAGATAAAGAAAGGTAATGGTGGCAGTTCAGTGGTGGAAATAGAAAGAGCTTTGAAGTTGGGAAGAGGAGTTTATGGAAAAGTAATGAAGAAGCAAGCAATGAAGAGTCTTCTTGGCCTTGGCATTCTTGAACCCAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SET

Protein Analysis

538

Amino Acids

60.5

Weight (kDa)

8.09

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 193 - 368 3.3e-14 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26760
fragaria_vesca FvH4_4g31050
malus_domestica MD13G1069000.v1.1
prunus_persica Prupe.1G278000_v2.0.a1
pyrus_communis pycom13g06180 pycom16g06040
rosa_chinensis RchiOBHm_Chr4g0439431
rosa_laevigata RLG00000006273
rosa_multiflora Rmu_sc0007276.1_g000002
rosa_roxburghii Rroxscaffold_5G00380340
rosa_rugosa Rorug04G0318100
rosa_samantha Rh4AG369400 Rh4BG381200 Rh4CG395800 Rh4DG375900
rosa_wichuraiana Rw4G031490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 438
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 2 cut(s) 426, 808
AccII CGCG 1 cut(s) 229
AccIII TCCGGA 1 cut(s) 520
AciI CCGC 1 cut(s) 1268
AclWI GGATC 5 cut(s) 51, 225, 383, 1387, 1400
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 1 cut(s) 337
AcuI CTGAAG 1 cut(s) 355
AfaI GTAC 7 cut(s) 350, 428, 536, 772, 854, 984, 1328
AfiI CCNNNNNNNGG 3 cut(s) 202, 1023, 1402
AflIII ACRYGT 1 cut(s) 755
AgsI TTSAA 7 cut(s) 262, 304, 343, 1065, 1459, 1528, 1604
AjnI CCWGG 1 cut(s) 201
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
Alw21I GWGCWC 4 cut(s) 75, 296, 410, 846
Alw26I GTCTC 1 cut(s) 237
AlwI GGATC 5 cut(s) 51, 225, 383, 1387, 1400
Ama87I CYCGRG 1 cut(s) 1280
Aor13HI TCCGGA 1 cut(s) 520
AoxI GGCC 3 cut(s) 204, 738, 1588
ApeKI GCWGC 4 cut(s) 46, 49, 1265, 1349
ApoI RAATTY 1 cut(s) 337
AseI ATTAAT 2 cut(s) 630, 819
Asp718I GGTACC 1 cut(s) 426
AspLEI GCGC 2 cut(s) 229, 1134
AspS9I GGNCC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 666
AsuHPI GGTGA 4 cut(s) 505, 797, 1094, 1318
AsuII TTCGAA 1 cut(s) 1242
AvaI CYCGRG 1 cut(s) 1280
AxyI CCTNAGG 1 cut(s) 393
BalI TGGCCA 1 cut(s) 740
BamHI GGATCC 1 cut(s) 1392
BanI GGYRCC 2 cut(s) 426, 808
BanII GRGCYC 2 cut(s) 75, 410
BbsI GAAGAC 3 cut(s) 713, 1393, 1574
Bbv12I GWGCWC 4 cut(s) 75, 296, 410, 846
BbvI GCAGC 4 cut(s) 58, 61, 1252, 1336
BccI CCATC 6 cut(s) 721, 791, 1228, 1246, 1396, 1456
BcgI CGANNNNNNTGC 2 cut(s) 430, 464
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 495
BcnI CCSGG 1 cut(s) 666
BcoDI GTCTC 1 cut(s) 237
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 3 cut(s) 431, 1171, 1273
BfuAI ACCTGC 1 cut(s) 438
BisI GCNGC 4 cut(s) 47, 50, 1266, 1350
BlpI GCTNAGC 2 cut(s) 381, 513
BlsI GCNGC 4 cut(s) 48, 51, 1267, 1351
BmcAI AGTACT 1 cut(s) 772
Bme1390I CCNGG 2 cut(s) 203, 666
BmeT110I CYCGRG 1 cut(s) 1280
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 4 cut(s) 428, 525, 810, 1394
BmrFI CCNGG 2 cut(s) 203, 666
BmsI GCATC 1 cut(s) 865
BpiI GAAGAC 3 cut(s) 713, 1393, 1574
BpmI CTGGAG 1 cut(s) 489
Bpu1102I GCTNAGC 2 cut(s) 381, 513
Bpu14I TTCGAA 1 cut(s) 1242
BpuEI CTTGAG 2 cut(s) 824, 1351
BpuMI CCSGG 1 cut(s) 666
BsaJI CCNNGG 4 cut(s) 354, 735, 1410, 1591
BsaWI WCCGGW 1 cut(s) 520
BsaXI ACNNNNNCTCC 4 cut(s) 335, 365, 534, 564
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 1023, 1402
Bse118I RCCGGY 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 472
Bse21I CCTNAGG 1 cut(s) 393
Bse3DI GCAATG 1 cut(s) 1578
BseAI TCCGGA 1 cut(s) 520
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 4 cut(s) 354, 735, 1410, 1591
BseGI GGATG 4 cut(s) 685, 802, 907, 1257
BseLI CCNNNNNNNGG 3 cut(s) 202, 1023, 1402
BseMI GCAATG 1 cut(s) 1578
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 2 cut(s) 1100, 1554
BseXI GCAGC 4 cut(s) 58, 61, 1252, 1336
BseYI CCCAGC 2 cut(s) 611, 1607
Bsh1236I CGCG 1 cut(s) 229
BshFI GGCC 3 cut(s) 206, 740, 1590
BshNI GGYRCC 2 cut(s) 426, 808
BsiHKAI GWGCWC 4 cut(s) 75, 296, 410, 846
BsiHKCI CYCGRG 1 cut(s) 1280
BsiSI CCGG 3 cut(s) 521, 527, 665
BslFI GGGAC 2 cut(s) 178, 1055
BslI CCNNNNNNNGG 3 cut(s) 202, 1023, 1402
BsmAI GTCTC 1 cut(s) 237
BsmFI GGGAC 2 cut(s) 178, 1055
BsmI GAATGC 2 cut(s) 691, 1596
BsnI GGCC 3 cut(s) 206, 740, 1590
BsoBI CYCGRG 1 cut(s) 1280
Bsp119I TTCGAA 1 cut(s) 1242
Bsp1286I GDGCHC 4 cut(s) 75, 296, 410, 846
Bsp13I TCCGGA 1 cut(s) 520
Bsp143I GATC 5 cut(s) 56, 230, 388, 495, 1392
Bsp1720I GCTNAGC 2 cut(s) 381, 513
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 1 cut(s) 1268
BspANI GGCC 3 cut(s) 206, 740, 1590
BspEI TCCGGA 1 cut(s) 520
BspFNI CGCG 1 cut(s) 229
BspLI GGNNCC 4 cut(s) 428, 525, 810, 1394
BspMAI CTGCAG 2 cut(s) 435, 1175
BspMI ACCTGC 1 cut(s) 438
BspPI GGATC 5 cut(s) 51, 225, 383, 1387, 1400
BspQI GCTCTTC 1 cut(s) 30
BspT104I TTCGAA 1 cut(s) 1242
BspT107I GGYRCC 2 cut(s) 426, 808
BsrDI GCAATG 1 cut(s) 1578
BsrFI RCCGGY 1 cut(s) 526
BsrI ACTGG 1 cut(s) 472
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 4 cut(s) 354, 735, 1410, 1591
BssMI GATC 5 cut(s) 56, 230, 388, 495, 1392
BssT1I CCWWGG 2 cut(s) 354, 1591
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 956
Bst6I CTCTTC 3 cut(s) 30, 1531, 1571
BstBI TTCGAA 1 cut(s) 1242
BstC8I GCNNGC 2 cut(s) 528, 1569
BstDSI CCRYGG 3 cut(s) 354, 735, 1410
BstF5I GGATG 4 cut(s) 685, 802, 907, 1257
BstFNI CGCG 1 cut(s) 229
BstHHI GCGC 2 cut(s) 229, 1134
BstKTI GATC 5 cut(s) 59, 233, 391, 498, 1395
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 5 cut(s) 56, 230, 388, 495, 1392
BstMWI GCNNNNNNNGC 3 cut(s) 46, 523, 808
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 2 cut(s) 759, 1037
BstSCI CCNGG 2 cut(s) 201, 664
BstSFI CTRYAG 3 cut(s) 431, 1171, 1273
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 4 cut(s) 58, 61, 1252, 1336
BstV2I GAAGAC 3 cut(s) 713, 1393, 1574
BstX2I RGATCY 2 cut(s) 56, 1392
BstYI RGATCY 2 cut(s) 56, 1392
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 3 cut(s) 206, 740, 1590
BtgI CCRYGG 3 cut(s) 354, 735, 1410
BtsCI GGATG 4 cut(s) 685, 802, 907, 1257
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 4 cut(s) 278, 555, 961, 1509
BveI ACCTGC 1 cut(s) 438
Cac8I GCNNGC 2 cut(s) 528, 1569
CfoI GCGC 2 cut(s) 229, 1134
Cfr10I RCCGGY 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 205
Csp6I GTAC 7 cut(s) 349, 427, 535, 771, 853, 983, 1327
CviAII CATG 6 cut(s) 355, 756, 1008, 1034, 1058, 1291
CviQI GTAC 7 cut(s) 349, 427, 535, 771, 853, 983, 1327
DpnI GATC 5 cut(s) 58, 232, 390, 497, 1394
DpnII GATC 5 cut(s) 56, 230, 388, 495, 1392
EaeI YGGCCR 1 cut(s) 738
Eam1104I CTCTTC 3 cut(s) 30, 1531, 1571
EarI CTCTTC 3 cut(s) 30, 1531, 1571
Ecl136II GAGCTC 2 cut(s) 73, 408
Eco130I CCWWGG 2 cut(s) 354, 1591
Eco24I GRGCYC 2 cut(s) 75, 410
Eco53kI GAGCTC 2 cut(s) 73, 408
Eco57I CTGAAG 1 cut(s) 355
Eco81I CCTNAGG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 1280
EcoICRI GAGCTC 2 cut(s) 73, 408
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 354, 1591
EcoT38I GRGCYC 2 cut(s) 75, 410
ErhI CCWWGG 2 cut(s) 354, 1591
FaeI CATG 6 cut(s) 358, 759, 1011, 1037, 1061, 1294
FaqI GGGAC 2 cut(s) 178, 1055
FatI CATG 6 cut(s) 354, 755, 1007, 1033, 1057, 1290
FauNDI CATATG 1 cut(s) 1354
FbaI TGATCA 1 cut(s) 495
Fnu4HI GCNGC 4 cut(s) 47, 50, 1266, 1350
FokI GGATG 4 cut(s) 692, 809, 914, 1264
FriOI GRGCYC 2 cut(s) 75, 410
Fsp4HI GCNGC 4 cut(s) 47, 50, 1266, 1350
FspBI CTAG 1 cut(s) 23
GlaI GCGC 2 cut(s) 228, 1133
GluI GCNGC 4 cut(s) 47, 50, 1266, 1350
GsaI CCCAGC 2 cut(s) 615, 1611
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 3 cut(s) 206, 740, 1590
HapII CCGG 3 cut(s) 521, 527, 665
HhaI GCGC 2 cut(s) 229, 1134
Hin1II CATG 6 cut(s) 358, 759, 1011, 1037, 1061, 1294
Hin6I GCGC 2 cut(s) 227, 1132
HinP1I GCGC 2 cut(s) 227, 1132
HindIII AAGCTT 1 cut(s) 869
HpaII CCGG 3 cut(s) 521, 527, 665
HphI GGTGA 4 cut(s) 505, 797, 1094, 1318
Hpy188I TCNGA 7 cut(s) 56, 83, 242, 374, 487, 841, 1363
Hpy188III TCNNGA 7 cut(s) 234, 304, 521, 671, 750, 1065, 1601
Hpy99I CGWCG 1 cut(s) 598
HpyAV CCTTC 5 cut(s) 256, 1208, 1280, 1297, 1303
HpyCH4III ACNGT 1 cut(s) 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 523, 808
Hsp92II CATG 6 cut(s) 358, 759, 1011, 1037, 1061, 1294
HspAI GCGC 2 cut(s) 227, 1132
Kpn2I TCCGGA 1 cut(s) 520
KpnI GGTACC 1 cut(s) 430
KroI GCCGGC 1 cut(s) 526
KroNI GCCGGC 1 cut(s) 528
Ksp22I TGATCA 1 cut(s) 495
Kzo9I GATC 5 cut(s) 56, 230, 388, 495, 1392
LguI GCTCTTC 1 cut(s) 30
LmnI GCTCC 7 cut(s) 93, 189, 405, 470, 523, 841, 1087
Lsp1109I GCAGC 4 cut(s) 58, 61, 1252, 1336
LweI GCATC 1 cut(s) 865
MaeI CTAG 1 cut(s) 23
MalI GATC 5 cut(s) 58, 232, 390, 497, 1394
MboI GATC 5 cut(s) 56, 230, 388, 495, 1392
MflI RGATCY 2 cut(s) 56, 1392
MhlI GDGCHC 4 cut(s) 75, 296, 410, 846
MlsI TGGCCA 1 cut(s) 740
MluCI AATT 4 cut(s) 285, 337, 692, 907
MluNI TGGCCA 1 cut(s) 740
MlyI GAGTC 5 cut(s) 231, 301, 830, 1415, 1588
MmeI TCCRAC 1 cut(s) 1104
Mox20I TGGCCA 1 cut(s) 740
MroI TCCGGA 1 cut(s) 520
MroNI GCCGGC 1 cut(s) 526
MscI TGGCCA 1 cut(s) 740
MseI TTAA 5 cut(s) 420, 630, 819, 828, 890
MslI CAYNNNNRTG 1 cut(s) 147
Msp20I TGGCCA 1 cut(s) 740
MspI CCGG 3 cut(s) 521, 527, 665
MspR9I CCNGG 2 cut(s) 203, 666
Mva1269I GAATGC 2 cut(s) 691, 1596
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 3 cut(s) 46, 523, 808
NaeI GCCGGC 1 cut(s) 528
NciI CCSGG 1 cut(s) 666
NcoI CCATGG 1 cut(s) 354
NdeI CATATG 1 cut(s) 1354
NdeII GATC 5 cut(s) 56, 230, 388, 495, 1392
NgoMIV GCCGGC 1 cut(s) 526
NlaIII CATG 6 cut(s) 358, 759, 1011, 1037, 1061, 1294
NlaIV GGNNCC 4 cut(s) 428, 525, 810, 1394
NspI RCATGY 2 cut(s) 759, 1037
NspV TTCGAA 1 cut(s) 1242
PaeR7I CTCGAG 1 cut(s) 1280
PciI ACATGT 1 cut(s) 755
PciSI GCTCTTC 1 cut(s) 30
PctI GAATGC 2 cut(s) 691, 1596
PdiI GCCGGC 1 cut(s) 528
PfeI GAWTC 5 cut(s) 98, 731, 923, 1117, 1256
PkrI GCNGC 4 cut(s) 48, 51, 1267, 1351
PleI GAGTC 5 cut(s) 231, 301, 830, 1414, 1587
PpsI GAGTC 5 cut(s) 231, 301, 830, 1414, 1587
PscI ACATGT 1 cut(s) 755
PshBI ATTAAT 2 cut(s) 630, 819
Psp124BI GAGCTC 2 cut(s) 75, 410
Psp6I CCWGG 1 cut(s) 201
PspFI CCCAGC 2 cut(s) 611, 1607
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 4 cut(s) 428, 525, 810, 1394
PspPI GGNCC 1 cut(s) 205
PspXI VCTCGAGB 1 cut(s) 1280
PsrI GAACNNNNNNTAC 2 cut(s) 944, 976
PstI CTGCAG 2 cut(s) 435, 1175
PsuI RGATCY 2 cut(s) 56, 1392
RsaI GTAC 7 cut(s) 350, 428, 536, 772, 854, 984, 1328
RsaNI GTAC 7 cut(s) 349, 427, 535, 771, 853, 983, 1327
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 2 cut(s) 75, 410
SapI GCTCTTC 1 cut(s) 30
SaqAI TTAA 5 cut(s) 420, 630, 819, 828, 890
SatI GCNGC 4 cut(s) 47, 50, 1266, 1350
Sau3AI GATC 5 cut(s) 56, 230, 388, 495, 1392
Sau96I GGNCC 1 cut(s) 205
ScaI AGTACT 1 cut(s) 772
SchI GAGTC 5 cut(s) 231, 301, 830, 1415, 1588
ScrFI CCNGG 2 cut(s) 203, 666
SduI GDGCHC 4 cut(s) 75, 296, 410, 846
SfaNI GCATC 1 cut(s) 865
SfcI CTRYAG 3 cut(s) 431, 1171, 1273
Sfr274I CTCGAG 1 cut(s) 1280
SfuI TTCGAA 1 cut(s) 1242
SlaI CTCGAG 1 cut(s) 1280
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 3 cut(s) 803, 1280, 1330
SmoI CTYRAG 3 cut(s) 803, 1280, 1330
Sse9I AATT 4 cut(s) 285, 337, 692, 907
SsiI CCGC 1 cut(s) 1268
SspMI CTAG 1 cut(s) 23
SstI GAGCTC 2 cut(s) 75, 410
StyD4I CCNGG 2 cut(s) 201, 664
StyI CCWWGG 2 cut(s) 354, 1591
TaaI ACNGT 1 cut(s) 956
TasI AATT 4 cut(s) 285, 337, 692, 907
TatI WGTACW 2 cut(s) 534, 770
TfiI GAWTC 5 cut(s) 98, 731, 923, 1117, 1256
Tru1I TTAA 5 cut(s) 420, 630, 819, 828, 890
Tru9I TTAA 5 cut(s) 420, 630, 819, 828, 890
TscAI CASTG 4 cut(s) 285, 555, 961, 1509
TseI GCWGC 4 cut(s) 46, 49, 1265, 1349
TspDTI ATGAA 9 cut(s) 112, 171, 981, 985, 996, 1046, 1329, 1574, 1589
TspGWI ACGGA 5 cut(s) 662, 724, 1110, 1399, 1405
TspRI CASTG 4 cut(s) 285, 555, 961, 1509
VspI ATTAAT 2 cut(s) 630, 819
XapI RAATTY 1 cut(s) 337
XceI RCATGY 2 cut(s) 759, 1037
XhoI CTCGAG 1 cut(s) 1280
XspI CTAG 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.