Rh4CG015200

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0019407
Physical Location & Seq
Reverse (-)
2602663 .. 2629502
26840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG015200.1

Sequence Viewer

Length: 351 bp
ATGGAGCGCCAAATGCCTCGTCAGTTCTCCTCACTTCATCCGAAGAGTACATACGTGCATTTGGACTACCTTGATGCTGGAGCAAACGTCATAATAACCGCATCTTATCAGGCCACAATTCAGGGTTTTGAGGCCAAAGGTTTCTTAAAGGAAGAAGCCAAAGCCTTGCTCAGGAAAAGTGTAGAAATTGCAATCGAGGCACGAGAAATTTACTATGACAAGTGCACCAAAGGTTCTTGGGATTTTGCGGACACAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCAGCATCTGCGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGTATAGAGGAATAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

116

Amino Acids

12.85

Weight (kDa)

6.29

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 19 - 112 8.7e-17 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019407)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0387071
rosa_multiflora Rmu_sc0006648.1_g000010
rosa_samantha Rh4CG014400 Rh4CG014700 Rh4CG015200 Rh4CG015700 Rh4CG016100 Rh4DG011400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 99, 248, 296
AcsI RAATTY 1 cut(s) 207
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 171
AgsI TTSAA 1 cut(s) 267
AluBI AGCT 1 cut(s) 303
AluI AGCT 1 cut(s) 303
Alw21I GWGCWC 1 cut(s) 227
Alw44I GTGCAC 1 cut(s) 223
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 2 cut(s) 111, 132
ApaLI GTGCAC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 207
ArsI GACNNNNNNTTYG 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 262
AspLEI GCGC 1 cut(s) 9
BaeGI GKGCMC 1 cut(s) 227
BarI GAAGNNNNNNTAC 2 cut(s) 35, 67
BauI CACGAG 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 1 cut(s) 299
BccI CCATC 1 cut(s) 317
BfaI CTAG 1 cut(s) 281
BfoI RGCGCY 1 cut(s) 10
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
BmiI GGNNCC 2 cut(s) 310, 328
BmsI GCATC 3 cut(s) 64, 110, 299
BpmI CTGGAG 1 cut(s) 99
Bpu10I CCTNAGC 1 cut(s) 170
BsaAI YACGTR 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 1 cut(s) 171
Bse1I ACTGG 1 cut(s) 275
BseGI GGATG 2 cut(s) 37, 271
BseLI CCNNNNNNNGG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 184
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 1 cut(s) 299
BshFI GGCC 2 cut(s) 113, 134
BsiHKAI GWGCWC 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 171
BsnI GGCC 2 cut(s) 113, 134
Bsp1286I GDGCHC 1 cut(s) 227
BspACI CCGC 3 cut(s) 99, 248, 296
BspANI GGCC 2 cut(s) 113, 134
BspCNI CTCAG 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 310, 328
BsrI ACTGG 1 cut(s) 275
BssSI CACGAG 1 cut(s) 201
Bst2BI CACGAG 1 cut(s) 201
Bst6I CTCTTC 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 55
BstDEI CTNAG 1 cut(s) 170
BstENI CCTNNNNNAGG 1 cut(s) 169
BstF5I GGATG 2 cut(s) 37, 271
BstH2I RGCGCY 1 cut(s) 10
BstHHI GCGC 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 13, 197, 293, 317
BstSLI GKGCMC 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 299
BsuRI GGCC 2 cut(s) 113, 134
BtsCI GGATG 2 cut(s) 37, 271
CaiI CAGNNNCTG 1 cut(s) 293
CfoI GCGC 1 cut(s) 9
Csp6I GTAC 1 cut(s) 48
CviJI RGCY 8 cut(s) 113, 134, 158, 164, 262, 303, 311, 320
CviKI_1 RGCY 8 cut(s) 113, 134, 158, 164, 262, 303, 311, 320
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 170
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
EcoNI CCTNNNNNAGG 1 cut(s) 169
FaiI YATR 5 cut(s) 52, 92, 216, 306, 336
FauI CCCGC 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 2 cut(s) 24, 258
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 1 cut(s) 281
GlaI GCGC 1 cut(s) 8
GluI GCNGC 1 cut(s) 288
GsuI CTGGAG 1 cut(s) 99
HaeII RGCGCY 1 cut(s) 10
HaeIII GGCC 2 cut(s) 113, 134
HhaI GCGC 1 cut(s) 9
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
Hpy166II GTNNAC 1 cut(s) 225
Hpy188I TCNGA 2 cut(s) 42, 331
Hpy188III TCNNGA 2 cut(s) 172, 267
Hpy8I GTNNAC 1 cut(s) 225
HpyAV CCTTC 1 cut(s) 273
HpyCH4IV ACGT 2 cut(s) 54, 87
HpyCH4V TGCA 4 cut(s) 58, 191, 225, 287
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 197, 293, 317
HpyF3I CTNAG 1 cut(s) 170
HpySE526I ACGT 2 cut(s) 54, 87
HspAI GCGC 1 cut(s) 7
LmnI GCTCC 3 cut(s) 4, 80, 308
LpnPI CCDG 6 cut(s) 63, 95, 107, 157, 240, 288
Lsp1109I GCAGC 1 cut(s) 299
LweI GCATC 3 cut(s) 64, 110, 299
MaeI CTAG 1 cut(s) 281
MaeII ACGT 2 cut(s) 54, 87
MboII GAAGA 2 cut(s) 55, 164
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 3 cut(s) 117, 186, 207
MnlI CCTC 5 cut(s) 27, 40, 124, 190, 332
MroXI GAANNNNTTC 1 cut(s) 262
MseI TTAA 1 cut(s) 146
MwoI GCNNNNNNNGC 4 cut(s) 13, 197, 293, 317
NlaIV GGNNCC 2 cut(s) 310, 328
PdmI GAANNNNTTC 1 cut(s) 262
PkrI GCNGC 1 cut(s) 289
Ppu21I YACGTR 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 310, 328
PstNI CAGNNNCTG 1 cut(s) 293
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 1 cut(s) 288
SduI GDGCHC 1 cut(s) 227
SetI ASST 6 cut(s) 57, 72, 90, 142, 235, 305
SfaNI GCATC 3 cut(s) 64, 110, 299
Sse9I AATT 3 cut(s) 117, 186, 207
SsiI CCGC 3 cut(s) 99, 248, 296
SspMI CTAG 1 cut(s) 281
TaiI ACGT 2 cut(s) 57, 90
TaqI TCGA 1 cut(s) 195
TasI AATT 3 cut(s) 117, 186, 207
TatI WGTACW 1 cut(s) 47
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 1 cut(s) 26
VneI GTGCAC 1 cut(s) 223
XagI CCTNNNNNAGG 1 cut(s) 169
XapI RAATTY 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 262
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.