Rh4CG015700

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0019407
Physical Location & Seq
Reverse (-)
2970000 .. 2999669
29670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG015700.1

Sequence Viewer

Length: 537 bp
ATGCCTCGTCAGTTCTCCTCACCTCGTCCGAAGGGTACATACGCCACAGTTCAGGGTTTTGAGGCCAAAGGTTTCTCTAAAAAAGAAGCCAAAGCCCTGCTCAGGAAAAGTGTAGAAATTGCAATCGAGGCACGAGAAAATTACTATGACAAATGCACCAAAGGTTCTTGGGATTTTGTGGACACAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCAGCCTCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGTATAGTTCTCGAGCTGCTAACCGTGGCATTCTCCTTGCTGATTCCCAGTCTGATGAGGTTTACAGCATCAGTGCTCAGTCTTCTTCAATCACTCGTGCCGCGTCTTCCTCCACGCCGCCGGCTTCTGTTTCAGCTCTCAAAAGATTGAAGAAGAGATTGAATGAATTGTGGCTTTCTGCAGTTCGGAAATGGCCTACAGGCACCTATCGCTACTTTGACTTCCATATTGACAATGATGACCTACGCTGCTACGCAGATGGAAGATGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

178

Amino Acids

19.71

Weight (kDa)

9.49

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 15 - 90 2.3e-06 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019407)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0387071
rosa_multiflora Rmu_sc0006648.1_g000010
rosa_samantha Rh4CG014400 Rh4CG014700 Rh4CG015200 Rh4CG015700 Rh4CG016100 Rh4DG011400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 464
AccII CGCG 1 cut(s) 365
AciI CCGC 2 cut(s) 363, 380
AfaI GTAC 1 cut(s) 37
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 4 cut(s) 198, 351, 412, 424
AluBI AGCT 3 cut(s) 234, 278, 398
AluI AGCT 3 cut(s) 234, 278, 398
Alw21I GWGCWC 1 cut(s) 340
AlwNI CAGNNNCTG 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 2 cut(s) 63, 455
ApeKI GCWGC 3 cut(s) 218, 278, 510
Asp700I GAANNNNTTC 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 12
AvaI CYCGRG 1 cut(s) 273
BanI GGYRCC 1 cut(s) 464
BarI GAAGNNNNNNTAC 2 cut(s) 23, 55
BauI CACGAG 2 cut(s) 132, 357
BbsI GAAGAC 2 cut(s) 336, 360
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 3 cut(s) 230, 265, 497
BccI CCATC 2 cut(s) 248, 515
BfaI CTAG 1 cut(s) 212
BfmI CTRYAG 2 cut(s) 441, 459
BglI GCCNNNNNGGC 1 cut(s) 248
BisI GCNGC 5 cut(s) 219, 279, 363, 380, 511
BlsI GCNGC 5 cut(s) 220, 280, 364, 381, 512
BmeT110I CYCGRG 1 cut(s) 273
BmiI GGNNCC 3 cut(s) 241, 259, 466
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 2 cut(s) 339, 518
BmuI ACTGGG 1 cut(s) 304
BpiI GAAGAC 2 cut(s) 336, 360
Bpu10I CCTNAGC 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 286
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 2 cut(s) 206, 310
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMII CTCAG 2 cut(s) 115, 353
BseNI ACTGG 2 cut(s) 206, 310
BseRI GAGGAG 1 cut(s) 7
BseXI GCAGC 3 cut(s) 230, 265, 497
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 2 cut(s) 65, 457
BshNI GGYRCC 1 cut(s) 464
BsiHKAI GWGCWC 1 cut(s) 340
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 102
BsmI GAATGC 1 cut(s) 291
BsnI GGCC 2 cut(s) 65, 457
BsoBI CYCGRG 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 340
BspACI CCGC 2 cut(s) 363, 380
BspANI GGCC 2 cut(s) 65, 457
BspCNI CTCAG 2 cut(s) 114, 352
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 3 cut(s) 241, 259, 466
BspMAI CTGCAG 1 cut(s) 445
BspT107I GGYRCC 1 cut(s) 464
BsrFI RCCGGY 1 cut(s) 382
BsrI ACTGG 2 cut(s) 206, 310
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 1 cut(s) 286
BssSI CACGAG 2 cut(s) 132, 357
Bst2BI CACGAG 2 cut(s) 132, 357
Bst4CI ACNGT 2 cut(s) 49, 287
Bst6I CTCTTC 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 101, 339
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 202
BstFNI CGCG 1 cut(s) 365
BstMWI GCNNNNNNNGC 3 cut(s) 128, 248, 471
BstSFI CTRYAG 2 cut(s) 441, 459
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 3 cut(s) 230, 265, 497
BstV2I GAAGAC 2 cut(s) 336, 360
BsuRI GGCC 2 cut(s) 65, 457
BtgI CCRYGG 1 cut(s) 286
BtsCI GGATG 1 cut(s) 202
BtsIMutI CAGTG 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 384
CaiI CAGNNNCTG 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 382
CseI GACGC 1 cut(s) 354
Csp6I GTAC 1 cut(s) 36
CviQI GTAC 1 cut(s) 36
DdeI CTNAG 2 cut(s) 101, 339
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
Eco88I CYCGRG 1 cut(s) 273
FaiI YATR 5 cut(s) 40, 147, 237, 267, 489
Fnu4HI GCNGC 5 cut(s) 219, 279, 363, 380, 511
FokI GGATG 1 cut(s) 189
Fsp4HI GCNGC 5 cut(s) 219, 279, 363, 380, 511
FspBI CTAG 1 cut(s) 212
GluI GCNGC 5 cut(s) 219, 279, 363, 380, 511
HaeIII GGCC 2 cut(s) 65, 457
HapII CCGG 1 cut(s) 383
HgaI GACGC 1 cut(s) 354
HinfI GANTC 1 cut(s) 305
HpaII CCGG 1 cut(s) 383
HphI GGTGA 1 cut(s) 12
Hpy166II GTNNAC 2 cut(s) 181, 325
Hpy188I TCNGA 4 cut(s) 30, 262, 316, 450
Hpy188III TCNNGA 3 cut(s) 103, 198, 273
Hpy8I GTNNAC 2 cut(s) 181, 325
HpyAV CCTTC 2 cut(s) 25, 204
HpyCH4III ACNGT 2 cut(s) 49, 287
HpyCH4V TGCA 5 cut(s) 122, 156, 218, 443, 531
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 248, 471
HpyF3I CTNAG 2 cut(s) 101, 339
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 8 cut(s) 38, 88, 110, 171, 219, 323, 396, 447
Lsp1109I GCAGC 3 cut(s) 230, 265, 497
LweI GCATC 2 cut(s) 339, 518
MaeI CTAG 1 cut(s) 212
MboII GAAGA 6 cut(s) 336, 339, 360, 424, 427, 537
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 3 cut(s) 117, 139, 428
MnlI CCTC 8 cut(s) 15, 28, 33, 55, 121, 232, 313, 382
MroNI GCCGGC 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 193
MspI CCGG 1 cut(s) 383
Mva1269I GAATGC 1 cut(s) 291
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 3 cut(s) 128, 248, 471
NaeI GCCGGC 1 cut(s) 384
NgoMIV GCCGGC 1 cut(s) 382
NlaIV GGNNCC 3 cut(s) 241, 259, 466
PaeR7I CTCGAG 1 cut(s) 273
PctI GAATGC 1 cut(s) 291
PdiI GCCGGC 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 193
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 5 cut(s) 220, 280, 364, 381, 512
PspN4I GGNNCC 3 cut(s) 241, 259, 466
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 224
RsaI GTAC 1 cut(s) 37
RsaNI GTAC 1 cut(s) 36
SatI GCNGC 5 cut(s) 219, 279, 363, 380, 511
SduI GDGCHC 1 cut(s) 340
SetI ASST 9 cut(s) 25, 73, 166, 236, 280, 324, 400, 470, 507
SfaNI GCATC 2 cut(s) 339, 518
SfcI CTRYAG 2 cut(s) 441, 459
Sfr274I CTCGAG 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 3 cut(s) 117, 139, 428
SsiI CCGC 2 cut(s) 363, 380
SspMI CTAG 1 cut(s) 212
TaaI ACNGT 2 cut(s) 49, 287
TaqI TCGA 2 cut(s) 126, 274
TasI AATT 3 cut(s) 117, 139, 428
TauI GCSGC 2 cut(s) 365, 382
TfiI GAWTC 1 cut(s) 305
TscAI CASTG 1 cut(s) 340
TseI GCWGC 3 cut(s) 218, 278, 510
TspDTI ATGAA 1 cut(s) 441
TspRI CASTG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 273
XmnI GAANNNNTTC 1 cut(s) 193
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.