Rh4DG012100

leucine-rich repeat extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0013807
Physical Location & Seq
Reverse (-)
1930814 .. 1932276
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG012100.1

Sequence Viewer

Length: 1083 bp
ATGAAATCTCCAAATCTCACTCTATCCGTTTTGATCATTCTTTCTATCACCTTCATTTCCAAACCCTCGCACCAAGTTTCTCATTCTCCTCAAACCTTTCCCGTTAATGCTAGACTTCAAAAGGCTTACCAAGCTCTCCAAGCGTGGAAACATGCCATCACCTCCGACCCGAATAACTTCACCGCCAACTGGTGCGGCCCAAATGTTTGCAGCTACACCGGAGTCTACTGTGCACAAGCCCTGGATGACCCTCACATCACTACCGTGGCTGGAGTAGACCTAAACCACGCCAACATAGCTGGTTCATTGCCTGAAGAACTCGGCCTCTTAGTCGACATAGCTGTCGTGCACATAAACTCAAACAACTTCTGTGGCACCATTCCTTCTAGCTTTCGTGGTCTTTACCTTCTTTACGAGCTCGATATTAGTAATAACCAATTCTCAGGTCCATTTCCTTCGTCTGTTCTCTGCCTTCCTTCACTCAAGTACCTAGACATTAGATACAACAACTTTGAAGGTCGAATTCCGCCTGCCCTTTTCGATCTAAAACTCGACGCCTTGTTCCTTAACAACAACAAGTTCCAATGTTCATTGCCTCAAAACATCGGCAACTCCACAGTCTCCGTGATCGTGTTAGCAAACAACGACTTCAAAGGTGGATTTCCTTCAAGTTTGGCCAAAATGAAGGATACATTAAACGAAGTTATACTCACGAATAGTGGACTAGAAGGGTGTTTGCCTTCAAATATTGGGTGCTTGGACAAATTGACAGTCTTTGATGTCAGCAACAATAAGTTGGACGGTTCATTGCCGGAGTCAATGGGTGGGATGAAGAGCTTGGAGCAACTGAATGTGGCAAATAACGGATTTTCTGGGCAGATTCCGGCAAGTGTTTGCTCTTTGCATAAGTTGGAGAGCTTTAACTCTTCAAATAACTACTTCTGTGGTCAACCAGAGATGTGTGTGAGGTTGAGAGAAACAGATGATAGGAAGAATTGCATTCCATATAGGCCATTCCAAAGATCAGCAAAAGAATGTGCAGCATTTCATTCCCATAATCATTTGGCCAACTGTGGAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005199
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

360

Amino Acids

39.3

Weight (kDa)

6.35

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 77 3.1e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 115 - 171 1.3e-07 Leucine rich repeat
LRR_8 PF13855 231 - 289 1.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22800
fragaria_vesca FvH4_4g00870
malus_domestica MD16G1198100.v1.1
prunus_persica Prupe.1G019600_v2.0.a1
pyrus_communis pycom16g16700
rosa_chinensis RchiOBHm_Chr4g0387491
rosa_laevigata RLG00000010118
rosa_multiflora Rmu_co8216490.1_g000001 Rmu_sc0009161.1_g000003 Rmu_sc0011414.1_g000003
rosa_roxburghii Rroxscaffold_5G00334170
rosa_rugosa Rorug03G0309900
rosa_samantha Rh4AG016200 Rh4BG010900 Rh4CG017400 Rh4DG012100
rosa_wichuraiana Rw4G001030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 374
AccI GTMKAC 3 cut(s) 225, 276, 333
AciI CCGC 3 cut(s) 183, 195, 527
AcoI YGGCCR 2 cut(s) 675, 1065
AcsI RAATTY 1 cut(s) 522
AcuI CTGAAG 1 cut(s) 333
AcyI GRCGYC 1 cut(s) 555
AfaI GTAC 1 cut(s) 488
AfiI CCNNNNNNNGG 1 cut(s) 189
AgsI TTSAA 6 cut(s) 119, 515, 652, 669, 744, 930
AjnI CCWGG 1 cut(s) 240
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 8 cut(s) 134, 213, 299, 341, 390, 418, 837, 918
AluI AGCT 8 cut(s) 134, 213, 299, 341, 390, 418, 837, 918
Alw21I GWGCWC 3 cut(s) 235, 351, 420
Alw26I GTCTC 1 cut(s) 625
Alw44I GTGCAC 2 cut(s) 231, 347
AoxI GGCC 5 cut(s) 196, 322, 675, 1010, 1065
ApaLI GTGCAC 2 cut(s) 231, 347
ApeKI GCWGC 2 cut(s) 210, 1040
ApoI RAATTY 1 cut(s) 522
ArsI GACNNNNNNTTYG 2 cut(s) 756, 788
Asp700I GAANNNNTTC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 197, 446
AsuHPI GGTGA 3 cut(s) 40, 151, 172
AvaII GGWCC 1 cut(s) 446
BaeGI GKGCMC 2 cut(s) 235, 351
BaeI ACNNNNGTAYC 2 cut(s) 470, 503
BalI TGGCCA 2 cut(s) 677, 1067
BanI GGYRCC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 420
Bbv12I GWGCWC 3 cut(s) 235, 351, 420
BbvI GCAGC 2 cut(s) 222, 1052
BccI CCATC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 242
BciVI GTATCC 1 cut(s) 682
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 625
BfaI CTAG 4 cut(s) 111, 387, 491, 725
BfuI GTATCC 1 cut(s) 682
BisI GCNGC 3 cut(s) 196, 211, 1041
BlsI GCNGC 3 cut(s) 197, 212, 1042
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 1 cut(s) 446
BmgT120I GGNCC 2 cut(s) 197, 446
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 467
BsaHI GRCGYC 1 cut(s) 555
BsaJI CCNNGG 2 cut(s) 240, 264
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse1I ACTGG 1 cut(s) 194
Bse3DI GCAATG 3 cut(s) 305, 590, 806
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 2 cut(s) 240, 264
BseGI GGATG 2 cut(s) 250, 834
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMI GCAATG 3 cut(s) 305, 590, 806
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 2 cut(s) 235, 351
BseXI GCAGC 2 cut(s) 222, 1052
BsgI GTGCAG 1 cut(s) 1059
BshFI GGCC 5 cut(s) 198, 324, 677, 1012, 1067
BshNI GGYRCC 1 cut(s) 374
BsiHKAI GWGCWC 3 cut(s) 235, 351, 420
BsiSI CCGG 3 cut(s) 219, 812, 884
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 625
BsmI GAATGC 1 cut(s) 999
BsnI GGCC 5 cut(s) 198, 324, 677, 1012, 1067
Bsp1286I GDGCHC 3 cut(s) 235, 351, 420
Bsp143I GATC 4 cut(s) 33, 541, 627, 1022
BspACI CCGC 3 cut(s) 183, 195, 527
BspANI GGCC 5 cut(s) 198, 324, 677, 1012, 1067
BspCNI CTCAG 1 cut(s) 455
BspLI GGNNCC 1 cut(s) 376
BspQI GCTCTTC 1 cut(s) 827
BspT107I GGYRCC 1 cut(s) 374
BsrDI GCAATG 3 cut(s) 305, 590, 806
BsrI ACTGG 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 240, 264
BssMI GATC 4 cut(s) 33, 541, 627, 1022
BssNI GRCGYC 1 cut(s) 555
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 6 cut(s) 230, 265, 619, 772, 803, 1073
Bst6I CTCTTC 2 cut(s) 827, 931
BstACI GRCGYC 1 cut(s) 555
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 2 cut(s) 328, 442
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 2 cut(s) 250, 834
BstKTI GATC 4 cut(s) 36, 544, 630, 1025
BstMAI GTCTC 1 cut(s) 625
BstMBI GATC 4 cut(s) 33, 541, 627, 1022
BstMWI GCNNNNNNNGC 3 cut(s) 131, 140, 296
BstNI CCWGG 1 cut(s) 242
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 240
BstSLI GKGCMC 2 cut(s) 235, 351
BstV1I GCAGC 2 cut(s) 222, 1052
BsuI GTATCC 1 cut(s) 682
BsuRI GGCC 5 cut(s) 198, 324, 677, 1012, 1067
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 2 cut(s) 250, 834
Cac8I GCNNGC 1 cut(s) 531
Cfr13I GGNCC 2 cut(s) 197, 446
CseI GACGC 1 cut(s) 563
Csp6I GTAC 1 cut(s) 487
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 1 cut(s) 152
CviQI GTAC 1 cut(s) 487
DdeI CTNAG 2 cut(s) 328, 442
DpnI GATC 4 cut(s) 35, 543, 629, 1024
DpnII GATC 4 cut(s) 33, 541, 627, 1022
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 2 cut(s) 675, 1065
Eam1104I CTCTTC 2 cut(s) 827, 931
EarI CTCTTC 2 cut(s) 827, 931
EciI GGCGGA 1 cut(s) 516
Ecl136II GAGCTC 1 cut(s) 418
Eco24I GRGCYC 1 cut(s) 420
Eco47I GGWCC 1 cut(s) 446
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 420
FaeI CATG 1 cut(s) 155
FaiI YATR 9 cut(s) 153, 296, 338, 353, 707, 906, 1006, 1008, 1056
FatI CATG 1 cut(s) 151
FbaI TGATCA 1 cut(s) 33
FblI GTMKAC 3 cut(s) 225, 276, 333
Fnu4HI GCNGC 3 cut(s) 196, 211, 1041
FokI GGATG 2 cut(s) 257, 841
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 3 cut(s) 196, 211, 1041
FspBI CTAG 4 cut(s) 111, 387, 491, 725
GluI GCNGC 3 cut(s) 196, 211, 1041
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 5 cut(s) 198, 324, 677, 1012, 1067
HapII CCGG 3 cut(s) 219, 812, 884
HgaI GACGC 1 cut(s) 563
Hin1I GRCGYC 1 cut(s) 555
Hin1II CATG 1 cut(s) 155
HincII GTYRAC 2 cut(s) 334, 950
HindII GTYRAC 2 cut(s) 334, 950
HinfI GANTC 3 cut(s) 222, 815, 880
HpaII CCGG 3 cut(s) 219, 812, 884
HphI GGTGA 3 cut(s) 40, 151, 172
Hpy166II GTNNAC 7 cut(s) 226, 233, 277, 334, 349, 722, 950
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 1 cut(s) 712
Hpy8I GTNNAC 7 cut(s) 226, 233, 277, 334, 349, 722, 950
Hpy99I CGWCG 1 cut(s) 557
HpyCH4III ACNGT 6 cut(s) 230, 265, 619, 772, 803, 1073
HpyCH4V TGCA 6 cut(s) 210, 233, 349, 904, 999, 1040
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 140, 296
HpyF3I CTNAG 2 cut(s) 328, 442
Hsp92I GRCGYC 1 cut(s) 555
Hsp92II CATG 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 4 cut(s) 33, 541, 627, 1022
LguI GCTCTTC 1 cut(s) 827
LmnI GCTCC 2 cut(s) 841, 1076
Lsp1109I GCAGC 2 cut(s) 222, 1052
MaeI CTAG 4 cut(s) 111, 387, 491, 725
MalI GATC 4 cut(s) 35, 543, 629, 1024
MboI GATC 4 cut(s) 33, 541, 627, 1022
MboII GAAGA 4 cut(s) 326, 844, 918, 1003
MhlI GDGCHC 3 cut(s) 235, 351, 420
MlsI TGGCCA 2 cut(s) 677, 1067
MluCI AATT 4 cut(s) 437, 522, 764, 994
MluNI TGGCCA 2 cut(s) 677, 1067
MlyI GAGTC 2 cut(s) 231, 824
MmeI TCCRAC 3 cut(s) 189, 777, 891
MnlI CCTC 7 cut(s) 76, 99, 172, 261, 335, 606, 960
Mox20I TGGCCA 2 cut(s) 677, 1067
MroXI GAANNNNTTC 1 cut(s) 176
MscI TGGCCA 2 cut(s) 677, 1067
MseI TTAA 4 cut(s) 105, 567, 695, 921
MslI CAYNNNNRTG 1 cut(s) 263
Msp20I TGGCCA 2 cut(s) 677, 1067
MspI CCGG 3 cut(s) 219, 812, 884
MspR9I CCNGG 1 cut(s) 242
Mva1269I GAATGC 1 cut(s) 999
MvaI CCWGG 1 cut(s) 242
MwoI GCNNNNNNNGC 3 cut(s) 131, 140, 296
NdeII GATC 4 cut(s) 33, 541, 627, 1022
NlaIII CATG 1 cut(s) 155
NlaIV GGNNCC 1 cut(s) 376
NmeAIII GCCGAG 1 cut(s) 300
NspI RCATGY 1 cut(s) 155
OliI CACNNNNGTG 1 cut(s) 263
PciSI GCTCTTC 1 cut(s) 827
PctI GAATGC 1 cut(s) 999
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 880
PkrI GCNGC 3 cut(s) 197, 212, 1042
PleI GAGTC 2 cut(s) 230, 823
PpsI GAGTC 2 cut(s) 230, 823
Psp124BI GAGCTC 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 2 cut(s) 197, 446
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
RseI CAYNNNNRTG 1 cut(s) 263
SacI GAGCTC 1 cut(s) 420
SalI GTCGAC 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 827
SaqAI TTAA 4 cut(s) 105, 567, 695, 921
SatI GCNGC 3 cut(s) 196, 211, 1041
Sau3AI GATC 4 cut(s) 33, 541, 627, 1022
Sau96I GGNCC 2 cut(s) 197, 446
SchI GAGTC 2 cut(s) 231, 824
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 3 cut(s) 235, 351, 420
SinI GGWCC 1 cut(s) 446
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 4 cut(s) 437, 522, 764, 994
SsiI CCGC 3 cut(s) 183, 195, 527
SspI AATATT 1 cut(s) 748
SspMI CTAG 4 cut(s) 111, 387, 491, 725
SstI GAGCTC 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 240
TaaI ACNGT 6 cut(s) 230, 265, 619, 772, 803, 1073
TaqI TCGA 5 cut(s) 333, 420, 520, 540, 552
TasI AATT 4 cut(s) 437, 522, 764, 994
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 1 cut(s) 880
Tru1I TTAA 4 cut(s) 105, 567, 695, 921
Tru9I TTAA 4 cut(s) 105, 567, 695, 921
TseI GCWGC 2 cut(s) 210, 1040
TspDTI ATGAA 8 cut(s) 17, 43, 294, 579, 698, 795, 845, 1037
TspGWI ACGGA 3 cut(s) 16, 613, 879
VneI GTGCAC 2 cut(s) 231, 347
VpaK11BI GGWCC 1 cut(s) 446
XapI RAATTY 1 cut(s) 522
XceI RCATGY 1 cut(s) 155
XmiI GTMKAC 3 cut(s) 225, 276, 333
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 4 cut(s) 111, 387, 491, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.