Rh4DG055800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018678
Physical Location & Seq
Reverse (-)
8646449 .. 8646862
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG055800.1

Sequence Viewer

Length: 414 bp
ATGGAGCCACTGGCACAAGGACTTCGACGTCCAGCACAAGCTCCTTTCACCGGAGGCTTCGGGTTTCGATTCCGGGGAGAGAGAGATGTGTGGTCCGATTCCGGTTCCGGCGACGAGGTCGAAGAATCCGATGATTTGGGCGTGGGATCGCCGGGAGCGGGAGCCGGAGGAATCTGGAGCGTAGATTCAGAGGGAACCATCGGAGCAACCGAGGAGGAGCTTAGGGCCGTAGGATTCGATGGCTTCGATCTTGGTAGGGCAGTCGGAGATGAGCTCGACAGAATCGTAGCCACTGTTAAACATGGTGGTGGTTCGGTTTGGAACTTTATATTCGTCGCAGTGTTGGAGCGAGCTGGTATGATTATAGAGGTGGTGCGTGAAACGACGTCGTCAGGCGCTGTTTCTTTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.31

Weight (kDa)

4.33

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 27
AatII GACGTC 2 cut(s) 31, 389
AccBSI CCGCTC 1 cut(s) 158
AciI CCGC 1 cut(s) 158
AclWI GGATC 1 cut(s) 154
AcyI GRCGYC 2 cut(s) 28, 386
AfiI CCNNNNNNNGG 2 cut(s) 50, 158
AluBI AGCT 4 cut(s) 41, 220, 274, 353
AluI AGCT 4 cut(s) 41, 220, 274, 353
Alw21I GWGCWC 1 cut(s) 276
AlwI GGATC 1 cut(s) 154
AlwNI CAGNNNCTG 1 cut(s) 398
AoxI GGCC 1 cut(s) 225
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 2 cut(s) 93, 225
AsuC2I CCSGG 2 cut(s) 74, 153
AsuHPI GGTGA 1 cut(s) 40
AvaII GGWCC 1 cut(s) 93
BanII GRGCYC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 276
BccI CCATC 2 cut(s) 206, 233
BceAI ACGGC 1 cut(s) 212
BcnI CCSGG 2 cut(s) 74, 153
BfoI RGCGCY 1 cut(s) 399
Bme1390I CCNGG 2 cut(s) 74, 153
Bme18I GGWCC 1 cut(s) 93
BmgT120I GGNCC 2 cut(s) 93, 225
BmiI GGNNCC 4 cut(s) 6, 106, 163, 196
BmrFI CCNGG 2 cut(s) 74, 153
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 221
BpuMI CCSGG 2 cut(s) 74, 153
BsaHI GRCGYC 2 cut(s) 28, 386
BsaJI CCNNGG 2 cut(s) 73, 210
BsaWI WCCGGW 2 cut(s) 50, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 158
Bse1I ACTGG 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 73, 210
BseLI CCNNNNNNNGG 2 cut(s) 50, 158
BseNI ACTGG 1 cut(s) 15
BseRI GAGGAG 2 cut(s) 227, 230
BshFI GGCC 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 6 cut(s) 51, 73, 102, 108, 152, 165
BslI CCNNNNNNNGG 2 cut(s) 50, 158
BsnI GGCC 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 2 cut(s) 146, 247
BspACI CCGC 1 cut(s) 158
BspANI GGCC 1 cut(s) 227
BspLI GGNNCC 4 cut(s) 6, 106, 163, 196
BspPI GGATC 1 cut(s) 154
BsrBI CCGCTC 1 cut(s) 158
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 73, 210
BssMI GATC 2 cut(s) 146, 247
BssNI GRCGYC 2 cut(s) 28, 386
Bst4CI ACNGT 1 cut(s) 295
BstACI GRCGYC 2 cut(s) 28, 386
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 2 cut(s) 149, 250
BstMBI GATC 2 cut(s) 146, 247
BstSCI CCNGG 2 cut(s) 72, 151
BsuRI GGCC 1 cut(s) 227
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 3 cut(s) 8, 291, 345
Cac8I GCNNGC 1 cut(s) 351
CaiI CAGNNNCTG 1 cut(s) 398
CfoI GCGC 1 cut(s) 398
Cfr13I GGNCC 2 cut(s) 93, 225
CviAII CATG 1 cut(s) 302
DdeI CTNAG 1 cut(s) 221
DpnI GATC 2 cut(s) 148, 249
DpnII GATC 2 cut(s) 146, 247
DrdI GACNNNNNNGTC 1 cut(s) 27
DseDI GACNNNNNNGTC 1 cut(s) 27
Ecl136II GAGCTC 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 274
EcoICRI GAGCTC 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 276
FaeI CATG 1 cut(s) 305
FaiI YATR 4 cut(s) 303, 329, 359, 365
FatI CATG 1 cut(s) 301
FauI CCCGC 1 cut(s) 151
FriOI GRGCYC 1 cut(s) 276
GlaI GCGC 1 cut(s) 397
GsuI CTGGAG 1 cut(s) 196
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 1 cut(s) 227
HapII CCGG 6 cut(s) 51, 73, 102, 108, 152, 165
HhaI GCGC 1 cut(s) 398
Hin1I GRCGYC 2 cut(s) 28, 386
Hin1II CATG 1 cut(s) 305
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HinfI GANTC 7 cut(s) 69, 98, 125, 171, 185, 234, 282
HpaII CCGG 6 cut(s) 51, 73, 102, 108, 152, 165
HphI GGTGA 1 cut(s) 40
Hpy188I TCNGA 5 cut(s) 97, 130, 190, 203, 266
Hpy188III TCNNGA 1 cut(s) 175
Hpy99I CGWCG 5 cut(s) 30, 116, 338, 388, 391
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 2 cut(s) 28, 386
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 2 cut(s) 28, 386
Hsp92I GRCGYC 2 cut(s) 28, 386
Hsp92II CATG 1 cut(s) 305
HspAI GCGC 1 cut(s) 396
Kzo9I GATC 2 cut(s) 146, 247
LmnI GCTCC 8 cut(s) 4, 46, 155, 161, 177, 203, 217, 346
MaeII ACGT 2 cut(s) 28, 386
MalI GATC 2 cut(s) 148, 249
MbiI CCGCTC 1 cut(s) 158
MboI GATC 2 cut(s) 146, 247
MboII GAAGA 1 cut(s) 134
MhlI GDGCHC 1 cut(s) 276
MmeI TCCRAC 2 cut(s) 244, 324
MnlI CCTC 7 cut(s) 47, 109, 161, 184, 205, 208, 361
MseI TTAA 2 cut(s) 297, 412
MslI CAYNNNNRTG 1 cut(s) 306
MspI CCGG 6 cut(s) 51, 73, 102, 108, 152, 165
MspR9I CCNGG 2 cut(s) 74, 153
NciI CCSGG 2 cut(s) 74, 153
NdeII GATC 2 cut(s) 146, 247
NlaIII CATG 1 cut(s) 305
NlaIV GGNNCC 4 cut(s) 6, 106, 163, 196
PcsI WCGNNNNNNNCGW 4 cut(s) 126, 207, 243, 282
PfeI GAWTC 7 cut(s) 69, 98, 125, 171, 185, 234, 282
PflFI GACNNNGTC 2 cut(s) 116, 388
Psp124BI GAGCTC 1 cut(s) 276
PspN4I GGNNCC 4 cut(s) 6, 106, 163, 196
PspPI GGNCC 2 cut(s) 93, 225
PstNI CAGNNNCTG 1 cut(s) 398
PsyI GACNNNGTC 2 cut(s) 116, 388
RseI CAYNNNNRTG 1 cut(s) 306
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 2 cut(s) 297, 412
Sau3AI GATC 2 cut(s) 146, 247
Sau96I GGNCC 2 cut(s) 93, 225
ScrFI CCNGG 2 cut(s) 74, 153
SduI GDGCHC 1 cut(s) 276
SetI ASST 8 cut(s) 31, 43, 120, 222, 276, 355, 372, 389
SinI GGWCC 1 cut(s) 93
SmiMI CAYNNNNRTG 1 cut(s) 306
SsiI CCGC 1 cut(s) 158
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 2 cut(s) 72, 151
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 2 cut(s) 31, 389
TaqI TCGA 6 cut(s) 25, 67, 120, 237, 246, 276
TfiI GAWTC 7 cut(s) 69, 98, 125, 171, 185, 234, 282
Tru1I TTAA 2 cut(s) 297, 412
Tru9I TTAA 2 cut(s) 297, 412
TscAI CASTG 3 cut(s) 15, 298, 345
TspRI CASTG 3 cut(s) 15, 298, 345
Tth111I GACNNNGTC 2 cut(s) 116, 388
VpaK11BI GGWCC 1 cut(s) 93
ZraI GACGTC 2 cut(s) 29, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.