Rh5DG525100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018678
Physical Location & Seq
Reverse (-)
81298547 .. 81308916
10370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG525100.1

Sequence Viewer

Length: 597 bp
ATGTTTTTTGGTAGCGATTCCGGTGGTCGTCTTTGTACTCCGGTAGCCATGTTGTTCGGGACTGAAGGAACTCGTTTTGACAGCGCCGGCTTGCGGTTTCGATGGAGGCAAAGGCTATGTGCTGTTGGGTCGTGCAATCTGGTGTGGCAACGTCAAGGCGGCGTACGGGATGGGTCGTGGCGGCGTGCGATGTTGGTCGTGGCGGTGAGCGATGTTGGTCGTGGCGGTGAGCGATGTTGGTCTGAACGGCGTCAAGGCGGCGTGAGAAGGTGCAGCAGGGCTTCGGGTACAGCGGTGGTTGCCGGGTCAAGTCAAGGCTGCCTTGATGGGCCTTGGTTGGGTCATTCCTCTCTGGGCTGCGTTTGTTGGGCTTCTATGCTGCAGAATTCCTGGTTCATTCAAGTTTTCATCTTTTTCAATTCTCTCGAGAAAATTCCGGTTATTTCCTCGGGAATCTCTCCTAACGGCTTTTCCCTCCGCCGTGAAATTCCGTTTCCGTTTTCTATACACCTCCTCTGTTTCCGGTTAGATTCTCTTCGCCTGAATCGGAACACGCCGATTTTGATTCCAAAGTACATGGTGACATGCGTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.01

Weight (kDa)

10.31

Isoelectric Point (pI)

56.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 94, 159, 181, 203, 225, 258, 293, 478
AcsI RAATTY 3 cut(s) 385, 432, 486
AcuI CTGAAG 1 cut(s) 84
AcyI GRCGYC 1 cut(s) 250
AfaI GTAC 4 cut(s) 37, 165, 289, 575
AfiI CCNNNNNNNGG 2 cut(s) 93, 338
AgsI TTSAA 2 cut(s) 401, 418
AjnI CCWGG 1 cut(s) 389
Ama87I CYCGRG 2 cut(s) 425, 448
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 4 cut(s) 273, 318, 357, 379
ApoI RAATTY 3 cut(s) 385, 432, 486
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 329
AsuC2I CCSGG 1 cut(s) 304
AsuHPI GGTGA 3 cut(s) 217, 239, 592
AvaI CYCGRG 2 cut(s) 425, 448
BaeI ACNNNNGTAYC 2 cut(s) 279, 312
BbvI GCAGC 4 cut(s) 285, 305, 344, 366
BccI CCATC 3 cut(s) 96, 164, 320
BceAI ACGGC 3 cut(s) 263, 465, 481
BciT130I CCWGG 1 cut(s) 391
BcnI CCSGG 1 cut(s) 304
BfaI CTAG 1 cut(s) 595
BfmI CTRYAG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 87
BisI GCNGC 7 cut(s) 160, 182, 259, 274, 319, 358, 380
BlsI GCNGC 7 cut(s) 161, 183, 260, 275, 320, 359, 381
Bme1390I CCNGG 2 cut(s) 304, 391
BmeT110I CYCGRG 2 cut(s) 425, 448
BmgT120I GGNCC 1 cut(s) 329
BmrFI CCNGG 2 cut(s) 304, 391
BpuMI CCSGG 1 cut(s) 304
BsaHI GRCGYC 1 cut(s) 250
BsaJI CCNNGG 2 cut(s) 332, 447
BsaWI WCCGGW 4 cut(s) 20, 40, 436, 522
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 338
Bse118I RCCGGY 1 cut(s) 86
BseBI CCWGG 1 cut(s) 391
BseDI CCNNGG 2 cut(s) 332, 447
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 93, 338
BseRI GAGGAG 1 cut(s) 503
BseXI GCAGC 4 cut(s) 285, 305, 344, 366
BsgI GTGCAG 1 cut(s) 292
BshFI GGCC 1 cut(s) 331
BsiHKCI CYCGRG 2 cut(s) 425, 448
BsiSI CCGG 6 cut(s) 21, 41, 87, 303, 437, 523
BsiWI CGTACG 1 cut(s) 163
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 93, 338
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 1 cut(s) 331
BsoBI CYCGRG 2 cut(s) 425, 448
BspACI CCGC 8 cut(s) 94, 159, 181, 203, 225, 258, 293, 478
BspANI GGCC 1 cut(s) 331
BspMAI CTGCAG 1 cut(s) 384
BsrFI RCCGGY 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 2 cut(s) 332, 447
BssNI GRCGYC 1 cut(s) 250
BssT1I CCWWGG 1 cut(s) 332
Bst2UI CCWGG 1 cut(s) 391
Bst6I CTCTTC 1 cut(s) 540
BstACI GRCGYC 1 cut(s) 250
BstC8I GCNNGC 3 cut(s) 88, 92, 186
BstF5I GGATG 1 cut(s) 175
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstNI CCWGG 1 cut(s) 391
BstNSI RCATGY 1 cut(s) 588
BstSCI CCNGG 2 cut(s) 302, 389
BstSFI CTRYAG 1 cut(s) 380
BstV1I GCAGC 4 cut(s) 285, 305, 344, 366
BsuRI GGCC 1 cut(s) 331
BtgZI GCGATG 3 cut(s) 203, 225, 247
BtsCI GGATG 1 cut(s) 175
Cac8I GCNNGC 3 cut(s) 88, 92, 186
CfoI GCGC 1 cut(s) 86
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 329
CseI GACGC 1 cut(s) 239
Csp6I GTAC 4 cut(s) 36, 164, 288, 574
CviAII CATG 3 cut(s) 49, 577, 585
CviJI RGCY 9 cut(s) 47, 90, 115, 281, 318, 331, 357, 371, 468
CviKI_1 RGCY 9 cut(s) 47, 90, 115, 281, 318, 331, 357, 371, 468
CviQI GTAC 4 cut(s) 36, 164, 288, 574
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
EciI GGCGGA 1 cut(s) 467
Eco130I CCWWGG 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 84
Eco88I CYCGRG 2 cut(s) 425, 448
EcoRI GAATTC 1 cut(s) 385
EcoRII CCWGG 1 cut(s) 389
EcoT14I CCWWGG 1 cut(s) 332
ErhI CCWWGG 1 cut(s) 332
FaeI CATG 3 cut(s) 52, 580, 588
FaiI YATR 6 cut(s) 50, 118, 377, 506, 578, 586
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FaqI GGGAC 1 cut(s) 73
FatI CATG 3 cut(s) 48, 576, 584
Fnu4HI GCNGC 7 cut(s) 160, 182, 259, 274, 319, 358, 380
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 7 cut(s) 160, 182, 259, 274, 319, 358, 380
FspBI CTAG 1 cut(s) 595
GlaI GCGC 1 cut(s) 85
GluI GCNGC 7 cut(s) 160, 182, 259, 274, 319, 358, 380
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 1 cut(s) 331
HapII CCGG 6 cut(s) 21, 41, 87, 303, 437, 523
HgaI GACGC 1 cut(s) 239
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 250
Hin1II CATG 3 cut(s) 52, 580, 588
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HinfI GANTC 5 cut(s) 17, 453, 530, 544, 565
HpaII CCGG 6 cut(s) 21, 41, 87, 303, 437, 523
HphI GGTGA 3 cut(s) 217, 239, 592
Hpy188I TCNGA 2 cut(s) 244, 549
Hpy188III TCNNGA 4 cut(s) 58, 425, 427, 450
HpyAV CCTTC 2 cut(s) 59, 261
HpyCH4IV ACGT 1 cut(s) 151
HpyCH4V TGCA 3 cut(s) 135, 273, 382
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpySE526I ACGT 1 cut(s) 151
Hsp92I GRCGYC 1 cut(s) 250
Hsp92II CATG 3 cut(s) 52, 580, 588
HspAI GCGC 1 cut(s) 84
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Lsp1109I GCAGC 4 cut(s) 285, 305, 344, 366
MaeI CTAG 1 cut(s) 595
MaeII ACGT 1 cut(s) 151
MaeIII GTNAC 1 cut(s) 580
MboII GAAGA 1 cut(s) 527
MluCI AATT 4 cut(s) 385, 418, 432, 486
MnlI CCTC 6 cut(s) 99, 358, 457, 485, 521, 524
MroNI GCCGGC 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 6 cut(s) 21, 41, 87, 303, 437, 523
MspR9I CCNGG 2 cut(s) 304, 391
MvaI CCWGG 1 cut(s) 391
MwoI GCNNNNNNNGC 1 cut(s) 299
NaeI GCCGGC 1 cut(s) 88
NciI CCSGG 1 cut(s) 304
NgoMIV GCCGGC 1 cut(s) 86
NlaIII CATG 3 cut(s) 52, 580, 588
NmuCI GTSAC 1 cut(s) 580
NspI RCATGY 1 cut(s) 588
PaeR7I CTCGAG 1 cut(s) 425
PdiI GCCGGC 1 cut(s) 88
PfeI GAWTC 5 cut(s) 17, 453, 530, 544, 565
Pfl23II CGTACG 1 cut(s) 163
PkrI GCNGC 7 cut(s) 161, 183, 260, 275, 320, 359, 381
Psp6I CCWGG 1 cut(s) 389
PspGI CCWGG 1 cut(s) 389
PspLI CGTACG 1 cut(s) 163
PspPI GGNCC 1 cut(s) 329
PstI CTGCAG 1 cut(s) 384
RsaI GTAC 4 cut(s) 37, 165, 289, 575
RsaNI GTAC 4 cut(s) 36, 164, 288, 574
SatI GCNGC 7 cut(s) 160, 182, 259, 274, 319, 358, 380
Sau96I GGNCC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 304, 391
SetI ASST 3 cut(s) 154, 272, 513
SfcI CTRYAG 1 cut(s) 380
Sfr274I CTCGAG 1 cut(s) 425
SlaI CTCGAG 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 4 cut(s) 385, 418, 432, 486
SsiI CCGC 8 cut(s) 94, 159, 181, 203, 225, 258, 293, 478
SspMI CTAG 1 cut(s) 595
StyD4I CCNGG 2 cut(s) 302, 389
StyI CCWWGG 1 cut(s) 332
TaiI ACGT 1 cut(s) 154
TaqI TCGA 2 cut(s) 100, 426
TasI AATT 4 cut(s) 385, 418, 432, 486
TatI WGTACW 2 cut(s) 35, 573
TauI GCSGC 3 cut(s) 162, 184, 261
TfiI GAWTC 5 cut(s) 17, 453, 530, 544, 565
TseFI GTSAC 1 cut(s) 580
TseI GCWGC 4 cut(s) 273, 318, 357, 379
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 2 cut(s) 385, 397
TspGWI ACGGA 2 cut(s) 480, 486
XapI RAATTY 3 cut(s) 385, 432, 486
XceI RCATGY 1 cut(s) 588
XhoI CTCGAG 1 cut(s) 425
XspI CTAG 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.