Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0017437

Physical Location & Seq

Genomic Coordinates Reverse (-)

15650806 .. 15686419

Gene Structure 35614 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG102100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGGCTCGCCATCACCGTGGCCGACTTCTCCGTCACAAACGGAGCATTGAACTCAAGAGGGATAAGCTCGCCAAGCTGCAGCTTAAAGGCAAAGCTGAGGGACATTGTGGTGGCCTGTTACCCCTACCTGTCCGAGATGAGCTGGTTGCTTGCCGCCAGGCTCAATCACGCCGGCAGCGAACAAAACCACTGGTACCCTCCACTTGGGTTCCTCCTGATATTTTTCGCTATTACAGCAAGATGCTATTGACTCCTCATTTCCTTCAAAGGTTCAGCAAAGGAATAGTATCTATCGACAATCATCCTCATAAGGATATCATTGCAACTGGTGGGATTGATGAAAAGGTTGTGATCTTTGATTTAGCGTCAAACAAAGTCCTATCTACTCTTCATGGTCATTCAGACCGGGTTACTAGTGTTAGATTTGTGAGTCGTGATGATCTGTTCGTAACTGGGTCGGCTGACAAGACTGTTTGCATATGGAAAGGCTCTGATGATGGGAAGTATAAGTGCAACCTCACTTTGGAGAATCATACAGCTGAGGTGCGAGCTGTTACTGTACATCCCACAAATAACTACTTCGTGACTGCTTCTCTTGATAACACATGGTGTTTTTGTGATATTTCCAATGGAGAATGTCTGAGACAGGATGATTCAGTGTCTCAAGGTTATACATCTGCGGCCTTTCATCCAGATGGTGTCTTACTTGGAGTAGGCACCTCGGATCTCAAAATTTGGGATGTAAAAAGTCAGAGGGTGGTGAAAAGTCTTTGTCAACATGTTGGGGAAGTAACTTCCATCTCCTTCTCTGAAAATGGCAACTTTTTTGCGACTGCCGCTAGTGATGGTGTTAGATTGTGGGATAAGCGCAACTTAAGGAAATTCATATGGAGTGATCTTGGTACACGAACAAGCTGTGTTCTTGGGTGGGTTGAGTTTTGTGAATTTGATAGAAGTACTCTTGATAGACATACCATACTGAAAACCACACTTGTTGAGTTGGGTAGAACTTTGGCATTGGAAGAGTGTGCGTTGTGGATGCCCACGCGTACTGCGTTAGAGCTGCAACTGTCGTATACACTCCATCAGCAGAATCCGGTAGGACATACCGTACCCATTCATCTTCCTGTGATCAATCAAGTGTTTAGCAGCAACCATGCCGTGAAGATATCACCAAATTCCCCAGTGGCAAGACTGAGGCCACTTGCGGGAAAGCATATGCCTGGAGAGGTTGTTGCTAAGGTTTTTGTTGTTTTCTTGCAAGCTAATCTGTTCTCTAATCTGTTCTCTGATTTGCTTCCAAAGGCTGTAAGACGTCATTCTGCTCTTTGTGCTGGCTTGCTGCAAGAAAATGGATCCTTCTCATGGATGACCACAATAGCATTCAAATGGATCCTTCTCATTGTGCTGGCTATCATTCTCATGGATGCCCACAATAGCAAAGACAATTTTAATCATGCTTTGGGTGGATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0000349 GO:0000375 GO:0000377 GO:0000393 GO:0000398 GO:0000974 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008380 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022607 GO:0022613 GO:0022618 GO:0030312 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0034622 GO:0034641 GO:0036211 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0061630 GO:0061659 GO:0065003 GO:0070013 GO:0070534 GO:0070647 GO:0071006 GO:0071012 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0080008 GO:0090304 GO:0098542 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 ko04120 map03040 map04120

Pfam Domains

Protein Families

Prp19 U-box WD40

Protein Analysis

491

Amino Acids

54.93

Weight (kDa)

9.26

Isoelectric Point (pI)

37.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	87 - 163	9.8e-15	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	87 - 170	6.3e-10	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	88 - 202	1.1e-10	WDR36/Utp21 first beta-propeller
Beta-prop_THOC3	PF25174	88 - 144	7.1e-06	THOC3 beta-propeller domain
Beta-prop_DCAF12	PF23760	91 - 191	2.8e-07	DDB1- and CUL4-associated factor 12 beta propeller
WDR55	PF24796	91 - 160	3.6e-06	WDR55
WD40_Prp19	PF24814	92 - 302	2e-67	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	92 - 222	1.6e-13	CDC20/Fizzy WD40 domain
Beta-prop_EML	PF23409	93 - 289	8e-10	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	93 - 202	8.3e-19	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	95 - 202	6.5e-12	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	97 - 162	5.3e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	101 - 162	1.3e-07	MABP1/WDR62 second WD40 domain
WD40_Gbeta	PF25391	102 - 241	3e-09	G protein beta WD-40 repeat protein
WD40	PF00400	125 - 161	7.1e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	127 - 215	3.1e-07	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	129 - 219	2.9e-09	EIF3I
Beta-prop_THOC3	PF25174	133 - 294	6.8e-26	THOC3 beta-propeller domain
WDR55	PF24796	135 - 284	2.7e-11	WDR55
Beta-prop_WDR5	PF25175	151 - 292	8.3e-22	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	176 - 288	4.5e-12	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	183 - 283	4.8e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	187 - 284	3.9e-06	WDR90/POC16, second beta-propeller
WD40_CDC20-Fz	PF24807	222 - 293	9.3e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	223 - 289	8.4e-08	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017437)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr4g0402341 RchiOBHm_Chr4g0402351
rosa_laevigata	RLG00000009101
rosa_samantha	Rh4AG112100 Rh4BG104700 Rh4CG118900 Rh4DG101700 Rh4DG101800 Rh4DG101900 Rh4DG102000 Rh4DG102100 Rh4DG102500 Rh7DG037800

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	30
AatII	GACGTC	1 cut(s)	1318
Acc65I	GGTACC	1 cut(s)	193
AccB1I	GGYRCC	2 cut(s)	193, 716
AccI	GTMKAC	1 cut(s)	1076
AccII	CGCG	1 cut(s)	1048
AciI	CCGC	4 cut(s)	154, 680, 837, 1208
AclWI	GGATC	5 cut(s)	732, 1350, 1363, 1387, 1400
AcoI	YGGCCR	1 cut(s)	19
AcsI	RAATTY	4 cut(s)	732, 881, 944, 1177
AcyI	GRCGYC	1 cut(s)	1315
AdeI	CACNNNGTG	1 cut(s)	609
AfaI	GTAC	6 cut(s)	195, 561, 904, 958, 1051, 1113
AfiI	CCNNNNNNNGG	4 cut(s)	204, 523, 1208, 1365
AflII	CTTAAG	1 cut(s)	874
AflIII	ACRYGT	2 cut(s)	778, 1046
AgsI	TTSAA	3 cut(s)	50, 266, 1387
AhlI	ACTAGT	1 cut(s)	413
AjnI	CCWGG	2 cut(s)	156, 1222
Alw26I	GTCTC	2 cut(s)	637, 666
AlwI	GGATC	5 cut(s)	732, 1350, 1363, 1387, 1400
AoxI	GGCC	4 cut(s)	19, 112, 681, 1199
ApeKI	GCWGC	6 cut(s)	76, 79, 175, 1063, 1149, 1342
ApoI	RAATTY	4 cut(s)	732, 881, 944, 1177
Asp718I	GGTACC	1 cut(s)	193
AspLEI	GCGC	1 cut(s)	870
AsuC2I	CCSGG	1 cut(s)	407
AsuHPI	GGTGA	3 cut(s)	5, 772, 1164
BamHI	GGATCC	2 cut(s)	1355, 1392
BanI	GGYRCC	2 cut(s)	193, 716
BbvCI	CCTCAGC	2 cut(s)	96, 540
BbvI	GCAGC	6 cut(s)	63, 91, 187, 1050, 1161, 1329
BccI	CCATC	6 cut(s)	18, 491, 689, 806, 839, 1092
BceAI	ACGGC	1 cut(s)	1145
BciT130I	CCWGG	2 cut(s)	158, 1224
BclI	TGATCA	1 cut(s)	1131
BcnI	CCSGG	1 cut(s)	407
BcoDI	GTCTC	2 cut(s)	637, 666
BcuI	ACTAGT	1 cut(s)	413
BfaI	CTAG	2 cut(s)	414, 840
BfmI	CTRYAG	1 cut(s)	77
BfrI	CTTAAG	1 cut(s)	874
BisI	GCNGC	9 cut(s)	77, 80, 154, 176, 681, 837, 1064, 1150, 1343
BlsI	GCNGC	9 cut(s)	78, 81, 155, 177, 682, 838, 1065, 1151, 1344
BmcAI	AGTACT	1 cut(s)	958
Bme1390I	CCNGG	3 cut(s)	158, 407, 1224
BmiI	GGNNCC	5 cut(s)	195, 210, 718, 1357, 1394
BmrFI	CCNGG	3 cut(s)	158, 407, 1224
BmrI	ACTGGG	2 cut(s)	462, 1178
BmsI	GCATC	3 cut(s)	231, 1029, 1417
BmuI	ACTGGG	2 cut(s)	462, 1178
BpmI	CTGGAG	1 cut(s)	1245
Bpu10I	CCTNAGC	3 cut(s)	96, 540, 1239
BpuEI	CTTGAG	2 cut(s)	38, 648
BpuMI	CCSGG	1 cut(s)	407
BsaHI	GRCGYC	1 cut(s)	1315
BsaJI	CCNNGG	2 cut(s)	16, 720
BsaWI	WCCGGW	1 cut(s)	1096
BsaXI	ACNNNNNCTCC	2 cut(s)	1218, 1248
Bsc4I	CCNNNNNNNGG	4 cut(s)	204, 523, 1208, 1365
Bse118I	RCCGGY	1 cut(s)	171
Bse1I	ACTGG	4 cut(s)	195, 331, 457, 1184
Bse3DI	GCAATG	1 cut(s)	318
BseBI	CCWGG	2 cut(s)	158, 1224
BseDI	CCNNGG	2 cut(s)	16, 720
BseGI	GGATG	8 cut(s)	301, 562, 655, 688, 745, 1044, 1374, 1432
BseLI	CCNNNNNNNGG	4 cut(s)	204, 523, 1208, 1365
BseMI	GCAATG	1 cut(s)	318
BseMII	CTCAG	4 cut(s)	87, 531, 632, 1187
BseNI	ACTGG	4 cut(s)	195, 331, 457, 1184
BseRI	GAGGAG	1 cut(s)	243
BseXI	GCAGC	6 cut(s)	63, 91, 187, 1050, 1161, 1329
Bsh1236I	CGCG	1 cut(s)	1048
BshFI	GGCC	4 cut(s)	21, 114, 683, 1201
BshNI	GGYRCC	2 cut(s)	193, 716
BsiSI	CCGG	3 cut(s)	172, 406, 1097
BslFI	GGGAC	1 cut(s)	114
BslI	CCNNNNNNNGG	4 cut(s)	204, 523, 1208, 1365
BsmAI	GTCTC	2 cut(s)	637, 666
BsmFI	GGGAC	1 cut(s)	114
BsmI	GAATGC	1 cut(s)	1382
BsnI	GGCC	4 cut(s)	21, 114, 683, 1201
Bsp1407I	TGTACA	1 cut(s)	559
Bsp143I	GATC	8 cut(s)	351, 439, 724, 895, 1131, 1355, 1392, 1469
BspACI	CCGC	4 cut(s)	154, 680, 837, 1208
BspANI	GGCC	4 cut(s)	21, 114, 683, 1201
BspCNI	CTCAG	4 cut(s)	88, 532, 633, 1188
BspFNI	CGCG	1 cut(s)	1048
BspLI	GGNNCC	5 cut(s)	195, 210, 718, 1357, 1394
BspMAI	CTGCAG	1 cut(s)	81
BspPI	GGATC	5 cut(s)	732, 1350, 1363, 1387, 1400
BspT107I	GGYRCC	2 cut(s)	193, 716
BspTI	CTTAAG	1 cut(s)	874
BsrDI	GCAATG	1 cut(s)	318
BsrFI	RCCGGY	1 cut(s)	171
BsrGI	TGTACA	1 cut(s)	559
BsrI	ACTGG	4 cut(s)	195, 331, 457, 1184
BssAI	RCCGGY	1 cut(s)	171
BssECI	CCNNGG	2 cut(s)	16, 720
BssMI	GATC	8 cut(s)	351, 439, 724, 895, 1131, 1355, 1392, 1469
BssNAI	GTATAC	1 cut(s)	1077
BssNI	GRCGYC	1 cut(s)	1315
Bst1107I	GTATAC	1 cut(s)	1077
Bst2UI	CCWGG	2 cut(s)	158, 1224
Bst4CI	ACNGT	5 cut(s)	17, 472, 559, 1071, 1111
Bst6I	CTCTTC	2 cut(s)	393, 1017
BstACI	GRCGYC	1 cut(s)	1315
BstAFI	CTTAAG	1 cut(s)	874
BstAUI	TGTACA	1 cut(s)	559
BstC8I	GCNNGC	9 cut(s)	7, 69, 151, 173, 549, 1263, 1336, 1340, 1410
BstDEI	CTNAG	5 cut(s)	96, 540, 641, 1196, 1239
BstDSI	CCRYGG	1 cut(s)	16
BstF5I	GGATG	8 cut(s)	301, 562, 655, 688, 745, 1044, 1374, 1432
BstFNI	CGCG	1 cut(s)	1048
BstHHI	GCGC	1 cut(s)	870
BstKTI	GATC	8 cut(s)	354, 442, 727, 898, 1134, 1358, 1395, 1472
BstMAI	GTCTC	2 cut(s)	637, 666
BstMBI	GATC	8 cut(s)	351, 439, 724, 895, 1131, 1355, 1392, 1469
BstMWI	GCNNNNNNNGC	4 cut(s)	73, 234, 836, 1331
BstNI	CCWGG	2 cut(s)	158, 1224
BstNSI	RCATGY	1 cut(s)	782
BstSCI	CCNGG	3 cut(s)	156, 405, 1222
BstSFI	CTRYAG	1 cut(s)	77
BstUI	CGCG	1 cut(s)	1048
BstV1I	GCAGC	6 cut(s)	63, 91, 187, 1050, 1161, 1329
BstX2I	RGATCY	3 cut(s)	724, 1355, 1392
BstXI	CCANNNNNNTGG	1 cut(s)	17
BstYI	RGATCY	3 cut(s)	724, 1355, 1392
BstZ17I	GTATAC	1 cut(s)	1077
BsuRI	GGCC	4 cut(s)	21, 114, 683, 1201
BtgI	CCRYGG	1 cut(s)	16
BtsCI	GGATG	8 cut(s)	301, 562, 655, 688, 745, 1044, 1374, 1432
BtsIMutI	CAGTG	3 cut(s)	188, 663, 1191
Cac8I	GCNNGC	9 cut(s)	7, 69, 151, 173, 549, 1263, 1336, 1340, 1410
CfoI	GCGC	1 cut(s)	870
Cfr10I	RCCGGY	1 cut(s)	171
CseI	GACGC	1 cut(s)	354
Csp6I	GTAC	6 cut(s)	194, 560, 903, 957, 1050, 1112
CspCI	CAANNNNNGTGG	2 cut(s)	976, 1011
CviAII	CATG	7 cut(s)	392, 606, 779, 1157, 1365, 1423, 1457
CviQI	GTAC	6 cut(s)	194, 560, 903, 957, 1050, 1112
DdeI	CTNAG	5 cut(s)	96, 540, 641, 1196, 1239
DpnI	GATC	8 cut(s)	353, 441, 726, 897, 1133, 1357, 1394, 1471
DpnII	GATC	8 cut(s)	351, 439, 724, 895, 1131, 1355, 1392, 1469
DraIII	CACNNNGTG	1 cut(s)	609
DrdI	GACNNNNNNGTC	1 cut(s)	30
DseDI	GACNNNNNNGTC	1 cut(s)	30
EaeI	YGGCCR	1 cut(s)	19
Eam1104I	CTCTTC	2 cut(s)	393, 1017
EarI	CTCTTC	2 cut(s)	393, 1017
Eco32I	GATATC	2 cut(s)	316, 1170
EcoRII	CCWGG	2 cut(s)	156, 1222
EcoRV	GATATC	2 cut(s)	316, 1170
FaeI	CATG	7 cut(s)	395, 609, 782, 1160, 1368, 1426, 1460
FalI	AAGNNNNNCTT	2 cut(s)	857, 889
FaqI	GGGAC	1 cut(s)	114
FatI	CATG	7 cut(s)	391, 605, 778, 1156, 1364, 1422, 1456
FauI	CCCGC	1 cut(s)	1201
FauNDI	CATATG	3 cut(s)	479, 887, 1218
FbaI	TGATCA	1 cut(s)	1131
FblI	GTMKAC	1 cut(s)	1076
Fnu4HI	GCNGC	9 cut(s)	77, 80, 154, 176, 681, 837, 1064, 1150, 1343
FokI	GGATG	8 cut(s)	288, 549, 662, 675, 752, 1051, 1381, 1439
Fsp4HI	GCNGC	9 cut(s)	77, 80, 154, 176, 681, 837, 1064, 1150, 1343
FspBI	CTAG	2 cut(s)	414, 840
GlaI	GCGC	1 cut(s)	869
GluI	GCNGC	9 cut(s)	77, 80, 154, 176, 681, 837, 1064, 1150, 1343
GsuI	CTGGAG	1 cut(s)	1245
HaeIII	GGCC	4 cut(s)	21, 114, 683, 1201
HapII	CCGG	3 cut(s)	172, 406, 1097
HgaI	GACGC	1 cut(s)	354
HhaI	GCGC	1 cut(s)	870
Hin1I	GRCGYC	1 cut(s)	1315
Hin1II	CATG	7 cut(s)	395, 609, 782, 1160, 1368, 1426, 1460
Hin6I	GCGC	1 cut(s)	868
HinP1I	GCGC	1 cut(s)	868
HincII	GTYRAC	1 cut(s)	776
HindII	GTYRAC	1 cut(s)	776
HinfI	GANTC	5 cut(s)	250, 430, 529, 653, 1093
HpaII	CCGG	3 cut(s)	172, 406, 1097
HphI	GGTGA	3 cut(s)	5, 772, 1164
Hpy166II	GTNNAC	3 cut(s)	776, 905, 1077
Hpy188I	TCNGA	8 cut(s)	134, 403, 493, 642, 724, 753, 811, 1291
Hpy188III	TCNNGA	7 cut(s)	55, 215, 434, 583, 596, 692, 962
Hpy8I	GTNNAC	3 cut(s)	776, 905, 1077
HpyAV	CCTTC	4 cut(s)	272, 814, 1369, 1406
HpyCH4III	ACNGT	5 cut(s)	17, 472, 559, 1071, 1111
HpyCH4IV	ACGT	1 cut(s)	1315
HpyCH4V	TGCA	7 cut(s)	79, 323, 477, 513, 1066, 1261, 1345
HpyF10VI	GCNNNNNNNGC	4 cut(s)	73, 234, 836, 1331
HpyF3I	CTNAG	5 cut(s)	96, 540, 641, 1196, 1239
HpySE526I	ACGT	1 cut(s)	1315
Hsp92I	GRCGYC	1 cut(s)	1315
Hsp92II	CATG	7 cut(s)	395, 609, 782, 1160, 1368, 1426, 1460
HspAI	GCGC	1 cut(s)	868
KpnI	GGTACC	1 cut(s)	197
KroI	GCCGGC	1 cut(s)	171
KroNI	GCCGGC	1 cut(s)	173
Ksp22I	TGATCA	1 cut(s)	1131
Kzo9I	GATC	8 cut(s)	351, 439, 724, 895, 1131, 1355, 1392, 1469
LmnI	GCTCC	1 cut(s)	42
Lsp1109I	GCAGC	6 cut(s)	63, 91, 187, 1050, 1161, 1329
LweI	GCATC	3 cut(s)	231, 1029, 1417
MaeI	CTAG	2 cut(s)	414, 840
MaeII	ACGT	1 cut(s)	1315
MaeIII	GTNAC	7 cut(s)	32, 117, 409, 448, 553, 583, 790
MalI	GATC	8 cut(s)	353, 441, 726, 897, 1133, 1357, 1394, 1471
MboI	GATC	8 cut(s)	351, 439, 724, 895, 1131, 1355, 1392, 1469
MboII	GAAGA	4 cut(s)	380, 1034, 1115, 1177
MflI	RGATCY	3 cut(s)	724, 1355, 1392
MluCI	AATT	5 cut(s)	732, 881, 944, 1177, 1447
MluI	ACGCGT	1 cut(s)	1046
MlyI	GAGTC	2 cut(s)	244, 439
MroNI	GCCGGC	1 cut(s)	171
MseI	TTAA	3 cut(s)	84, 875, 1452
MslI	CAYNNNNRTG	5 cut(s)	15, 108, 693, 1387, 1421
MspA1I	CMGCKG	1 cut(s)	539
MspCI	CTTAAG	1 cut(s)	874
MspI	CCGG	3 cut(s)	172, 406, 1097
MspR9I	CCNGG	3 cut(s)	158, 407, 1224
Mva1269I	GAATGC	1 cut(s)	1382
MvaI	CCWGG	2 cut(s)	158, 1224
MvnI	CGCG	1 cut(s)	1048
MwoI	GCNNNNNNNGC	4 cut(s)	73, 234, 836, 1331
NaeI	GCCGGC	1 cut(s)	173
NciI	CCSGG	1 cut(s)	407
NdeI	CATATG	3 cut(s)	479, 887, 1218
NdeII	GATC	8 cut(s)	351, 439, 724, 895, 1131, 1355, 1392, 1469
NgoMIV	GCCGGC	1 cut(s)	171
NlaIII	CATG	7 cut(s)	395, 609, 782, 1160, 1368, 1426, 1460
NlaIV	GGNNCC	5 cut(s)	195, 210, 718, 1357, 1394
NmuCI	GTSAC	2 cut(s)	32, 583
NspI	RCATGY	1 cut(s)	782
PciI	ACATGT	1 cut(s)	778
PcsI	WCGNNNNNNNCGW	3 cut(s)	13, 175, 1052
PctI	GAATGC	1 cut(s)	1382
PdiI	GCCGGC	1 cut(s)	173
PfeI	GAWTC	3 cut(s)	529, 653, 1093
PkrI	GCNGC	9 cut(s)	78, 81, 155, 177, 682, 838, 1065, 1151, 1344
PleI	GAGTC	2 cut(s)	244, 438
PpsI	GAGTC	2 cut(s)	244, 438
PscI	ACATGT	1 cut(s)	778
Psp6I	CCWGG	2 cut(s)	156, 1222
PspGI	CCWGG	2 cut(s)	156, 1222
PspN4I	GGNNCC	5 cut(s)	195, 210, 718, 1357, 1394
PstI	CTGCAG	1 cut(s)	81
PsuI	RGATCY	3 cut(s)	724, 1355, 1392
PvuII	CAGCTG	1 cut(s)	539
RsaI	GTAC	6 cut(s)	195, 561, 904, 958, 1051, 1113
RsaNI	GTAC	6 cut(s)	194, 560, 903, 957, 1050, 1112
RseI	CAYNNNNRTG	5 cut(s)	15, 108, 693, 1387, 1421
SaqAI	TTAA	3 cut(s)	84, 875, 1452
SatI	GCNGC	9 cut(s)	77, 80, 154, 176, 681, 837, 1064, 1150, 1343
Sau3AI	GATC	8 cut(s)	351, 439, 724, 895, 1131, 1355, 1392, 1469
ScaI	AGTACT	1 cut(s)	958
SchI	GAGTC	2 cut(s)	244, 439
ScrFI	CCNGG	3 cut(s)	158, 407, 1224
SfaNI	GCATC	3 cut(s)	231, 1029, 1417
SfcI	CTRYAG	1 cut(s)	77
SmiMI	CAYNNNNRTG	5 cut(s)	15, 108, 693, 1387, 1421
SmlI	CTYRAG	3 cut(s)	53, 663, 874
SmoI	CTYRAG	3 cut(s)	53, 663, 874
SpeI	ACTAGT	1 cut(s)	413
Sse9I	AATT	5 cut(s)	732, 881, 944, 1177, 1447
SsiI	CCGC	4 cut(s)	154, 680, 837, 1208
SspMI	CTAG	2 cut(s)	414, 840
StyD4I	CCNGG	3 cut(s)	156, 405, 1222
TaaI	ACNGT	5 cut(s)	17, 472, 559, 1071, 1111
TaiI	ACGT	1 cut(s)	1318
TaqI	TCGA	1 cut(s)	294
TasI	AATT	5 cut(s)	732, 881, 944, 1177, 1447
TatI	WGTACW	2 cut(s)	559, 956
TauI	GCSGC	3 cut(s)	156, 683, 839
TfiI	GAWTC	3 cut(s)	529, 653, 1093
Tru1I	TTAA	3 cut(s)	84, 875, 1452
Tru9I	TTAA	3 cut(s)	84, 875, 1452
TscAI	CASTG	3 cut(s)	195, 663, 1191
TseFI	GTSAC	2 cut(s)	32, 583
TseI	GCWGC	6 cut(s)	76, 79, 175, 1063, 1149, 1342
Tsp45I	GTSAC	2 cut(s)	32, 583
TspDTI	ATGAA	5 cut(s)	354, 380, 677, 874, 1109
TspGWI	ACGGA	2 cut(s)	20, 55
TspRI	CASTG	3 cut(s)	195, 663, 1191
Vha464I	CTTAAG	1 cut(s)	874
XapI	RAATTY	4 cut(s)	732, 881, 944, 1177
XceI	RCATGY	1 cut(s)	782
XmiI	GTMKAC	1 cut(s)	1076
XspI	CTAG	2 cut(s)	414, 840
ZraI	GACGTC	1 cut(s)	1316
ZrmI	AGTACT	1 cut(s)	958

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.