Rh5AG181100

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018986
Physical Location & Seq
Forward (+)
20514847 .. 20515275
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG181100.1

Sequence Viewer

Length: 336 bp
ATGTGTTATGGAGGAATGGGTGTAGGTGGGGGCGTTATGGTTCTAGGTGGGATTAAATCCCCCTGGGATATGGTGCTCACCCATTTGGATCCTTTTCGCAGTCCATATTACAATATTGAATTGATGGAGATACATGTGGCTGGAAAGGCTTTGAAGTTTTACCCAAAGGTGTTTGATGAAAAGCGTGGAACTGTCTTGGATAGTGGAACGACGTACGCATACTCTCCAAAGGATGCTTTTATTACATTTAAGGATGCTGTAAGACTTCGTCTTTTGAGTTCCCTCATGTTTGTGTTTTTTTTTTGTTCTTTTGGTGATTCTTTTTGTATTATCTAA

Protein Analysis

111

Amino Acids

12.43

Weight (kDa)

7.7

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_C PF14541 37 - 88 9.5e-07 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 83, 96
AfaI GTAC 1 cut(s) 215
AflIII ACRYGT 1 cut(s) 133
AgsI TTSAA 2 cut(s) 119, 154
AjnI CCWGG 1 cut(s) 62
Alw21I GWGCWC 1 cut(s) 78
AlwI GGATC 2 cut(s) 83, 96
AsuHPI GGTGA 2 cut(s) 70, 326
BamHI GGATCC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 78
BccI CCATC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 64
BfaI CTAG 1 cut(s) 44
Bme1390I CCNGG 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 223, 244
BsaJI CCNNGG 2 cut(s) 62, 63
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 62, 63
BseGI GGATG 2 cut(s) 238, 259
BsiHKAI GWGCWC 1 cut(s) 78
BsiWI CGTACG 1 cut(s) 213
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 90
BspPI GGATC 2 cut(s) 83, 96
BssECI CCNNGG 2 cut(s) 62, 63
BssMI GATC 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 1 cut(s) 193
BstF5I GGATG 2 cut(s) 238, 259
BstKTI GATC 1 cut(s) 91
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
BtsCI GGATG 2 cut(s) 238, 259
Csp6I GTAC 1 cut(s) 214
CviAII CATG 2 cut(s) 134, 286
CviJI RGCY 2 cut(s) 140, 149
CviKI_1 RGCY 2 cut(s) 140, 149
CviQI GTAC 1 cut(s) 214
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 62
FaeI CATG 2 cut(s) 137, 289
FaiI YATR 7 cut(s) 9, 38, 71, 106, 135, 220, 287
FatI CATG 2 cut(s) 133, 285
FokI GGATG 2 cut(s) 245, 266
FspBI CTAG 1 cut(s) 44
Hin1II CATG 2 cut(s) 137, 289
HinfI GANTC 1 cut(s) 317
HphI GGTGA 2 cut(s) 70, 326
Hpy99I CGWCG 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 137, 289
Kzo9I GATC 1 cut(s) 88
LpnPI CCDG 3 cut(s) 49, 76, 126
LweI GCATC 2 cut(s) 223, 244
MaeI CTAG 1 cut(s) 44
MaeII ACGT 1 cut(s) 212
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 1 cut(s) 119
MnlI CCTC 2 cut(s) 5, 293
MseI TTAA 2 cut(s) 54, 249
MslI CAYNNNNRTG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeII GATC 1 cut(s) 88
NlaIII CATG 2 cut(s) 137, 289
NlaIV GGNNCC 1 cut(s) 90
NspI RCATGY 1 cut(s) 137
PasI CCCWGGG 1 cut(s) 63
PciI ACATGT 1 cut(s) 133
PfeI GAWTC 1 cut(s) 317
Pfl23II CGTACG 1 cut(s) 213
PflFI GACNNNGTC 1 cut(s) 267
PscI ACATGT 1 cut(s) 133
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspLI CGTACG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 90
PsuI RGATCY 1 cut(s) 88
PsyI GACNNNGTC 1 cut(s) 267
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 2 cut(s) 54, 249
Sau3AI GATC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 1 cut(s) 78
SetI ASST 4 cut(s) 28, 49, 171, 215
SfaNI GCATC 2 cut(s) 223, 244
SgeI CNNG 8 cut(s) 56, 75, 76, 146, 153, 197, 208, 298
SmiMI CAYNNNNRTG 1 cut(s) 290
Sse9I AATT 1 cut(s) 119
SspI AATATT 1 cut(s) 115
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 62
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 1 cut(s) 215
TasI AATT 1 cut(s) 119
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 2 cut(s) 54, 249
Tru9I TTAA 2 cut(s) 54, 249
TspDTI ATGAA 1 cut(s) 192
Tth111I GACNNNGTC 1 cut(s) 267
XceI RCATGY 1 cut(s) 137
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.