Rh5AG259000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012532
Physical Location & Seq
Reverse (-)
33806140 .. 33810030
3891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG259000.1

Sequence Viewer

Length: 501 bp
ATGGTTCAGAAGCTTGAAGCTATAAAGGGTGGTGGAGGATCCATCAGGGTAGGGGCCACCGGGACAGTCAGTTCCCTAATGACAAGAGAACTAGACTCCATCAAGGTTGAACCGCCGATGCCTATAGCTTCTTCCCGAATTAAACATCAAACAGCTCCTGTTTCTGTTCCATGTGGTGTTCCTACTCCAAAAAAGCTACCAAGGAAGTCATCTGACGAAGCCAGCAGCAGTGGAAGCAGCAACCACATGAATCAGAGACACCCTGACATTCCCCAGAAACCGAAAACTCATCTGAAAACTACTAGTCAAATCCCGATGCTCGACTCTCATAATATTGGTATGGATAAAACTCCTATTAGGCAGAAAGCTAACAAGAAAGGACCTAATATCGTTGAAGTTGTGGACATCAAATGTGGGGGCTCGGGTAGAGCATGGGCTGGCCCTGTAACGAATCGGCTCAAGAAGCTGAGCTTCTCAAAGCTCTCTGAGAGTGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

17.72

Weight (kDa)

10.2

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012532)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54850
fragaria_vesca FvH4_3g22210
malus_domestica MD11G1222100.v1.1
prunus_persica Prupe.4G209200_v2.0.a1 Prupe.4G209200_v2.0.a1 Prupe.4G209200_v2.0.a1 Prupe.4G209200_v2.0.a1
pyrus_communis pycom11g19590
rosa_chinensis RchiOBHm_Chr5g0038281
rosa_laevigata RLG00000033842
rosa_multiflora Rmu_sc0012148.1_g000002 Rmu_sc0015248.1_g000002
rosa_roxburghii Rroxscaffold_1G00042770
rosa_rugosa Rorug05G0171600
rosa_samantha Rh5AG259000 Rh5BG262700 Rh5CG295100 Rh5DG271700
rosa_wichuraiana Rw5G024240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 113
AclWI GGATC 2 cut(s) 33, 46
AgsI TTSAA 3 cut(s) 17, 110, 395
AhlI ACTAGT 1 cut(s) 302
AloI GAACNNNNNNTCC 2 cut(s) 55, 87
AluBI AGCT 9 cut(s) 13, 20, 128, 155, 196, 368, 466, 471, 481
AluI AGCT 9 cut(s) 13, 20, 128, 155, 196, 368, 466, 471, 481
Alw26I GTCTC 1 cut(s) 250
AlwI GGATC 2 cut(s) 33, 46
AlwNI CAGNNNCTG 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 421
AoxI GGCC 2 cut(s) 54, 439
ApeKI GCWGC 2 cut(s) 225, 237
AspS9I GGNCC 3 cut(s) 54, 380, 440
AsuC2I CCSGG 1 cut(s) 61
AvaI CYCGRG 1 cut(s) 421
AvaII GGWCC 1 cut(s) 380
BamHI GGATCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 422
BbvI GCAGC 2 cut(s) 237, 249
BccI CCATC 2 cut(s) 50, 107
BcnI CCSGG 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 250
BcuI ACTAGT 1 cut(s) 302
BfaI CTAG 2 cut(s) 92, 303
BfmI CTRYAG 1 cut(s) 123
BisI GCNGC 2 cut(s) 226, 238
BlpI GCTNAGC 1 cut(s) 467
BlsI GCNGC 2 cut(s) 227, 239
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 380
BmeT110I CYCGRG 1 cut(s) 421
BmgT120I GGNCC 3 cut(s) 54, 380, 440
BmiI GGNNCC 2 cut(s) 40, 55
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 2 cut(s) 108, 306
Bpu1102I GCTNAGC 1 cut(s) 467
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 200
BseDI CCNNGG 1 cut(s) 200
BseMII CTCAG 2 cut(s) 458, 477
BseXI GCAGC 2 cut(s) 237, 249
BshFI GGCC 2 cut(s) 56, 441
BsiHKCI CYCGRG 1 cut(s) 421
BsiSI CCGG 1 cut(s) 60
BslFI GGGAC 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 250
BsmFI GGGAC 1 cut(s) 76
BsnI GGCC 2 cut(s) 56, 441
BsoBI CYCGRG 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 422
Bsp143I GATC 1 cut(s) 38
Bsp1720I GCTNAGC 1 cut(s) 467
BspACI CCGC 1 cut(s) 113
BspANI GGCC 2 cut(s) 56, 441
BspCNI CTCAG 2 cut(s) 459, 478
BspLI GGNNCC 2 cut(s) 40, 55
BspPI GGATC 2 cut(s) 33, 46
BssECI CCNNGG 1 cut(s) 200
BssMI GATC 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 67
BstC8I GCNNGC 2 cut(s) 223, 439
BstDEI CTNAG 2 cut(s) 467, 486
BstKTI GATC 1 cut(s) 41
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 1 cut(s) 38
BstMWI GCNNNNNNNGC 2 cut(s) 234, 463
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 237, 249
BstX2I RGATCY 1 cut(s) 38
BstYI RGATCY 1 cut(s) 38
BsuRI GGCC 2 cut(s) 56, 441
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 223, 439
CaiI CAGNNNCTG 1 cut(s) 158
Cfr13I GGNCC 3 cut(s) 54, 380, 440
CviAII CATG 3 cut(s) 171, 247, 432
DdeI CTNAG 2 cut(s) 467, 486
DpnI GATC 1 cut(s) 40
DpnII GATC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 380
Eco88I CYCGRG 1 cut(s) 421
EcoO109I RGGNCCY 1 cut(s) 380
EcoT14I CCWWGG 1 cut(s) 200
EcoT38I GRGCYC 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 3 cut(s) 174, 250, 435
FaiI YATR 7 cut(s) 23, 125, 172, 248, 330, 341, 433
FalI AAGNNNNNCTT 2 cut(s) 455, 487
FaqI GGGAC 1 cut(s) 76
FatI CATG 3 cut(s) 170, 246, 431
Fnu4HI GCNGC 2 cut(s) 226, 238
FriOI GRGCYC 1 cut(s) 422
Fsp4HI GCNGC 2 cut(s) 226, 238
FspBI CTAG 2 cut(s) 92, 303
GluI GCNGC 2 cut(s) 226, 238
HaeIII GGCC 2 cut(s) 56, 441
HapII CCGG 1 cut(s) 60
Hin1II CATG 3 cut(s) 174, 250, 435
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 4 cut(s) 95, 250, 323, 451
HpaII CCGG 1 cut(s) 60
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 5 cut(s) 9, 214, 255, 294, 487
Hpy188III TCNNGA 3 cut(s) 135, 313, 460
Hpy8I GTNNAC 1 cut(s) 403
HpyCH4III ACNGT 1 cut(s) 67
HpyF10VI GCNNNNNNNGC 2 cut(s) 234, 463
HpyF3I CTNAG 2 cut(s) 467, 486
Hsp92II CATG 3 cut(s) 174, 250, 435
Kzo9I GATC 1 cut(s) 38
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 8 cut(s) 31, 73, 171, 235, 276, 287, 423, 456
Lsp1109I GCAGC 2 cut(s) 237, 249
LweI GCATC 2 cut(s) 108, 306
MaeI CTAG 2 cut(s) 92, 303
MaeIII GTNAC 1 cut(s) 445
MalI GATC 1 cut(s) 40
MboI GATC 1 cut(s) 38
MboII GAAGA 1 cut(s) 123
MflI RGATCY 1 cut(s) 38
MhlI GDGCHC 1 cut(s) 422
MluCI AATT 1 cut(s) 138
MlyI GAGTC 2 cut(s) 89, 317
MnlI CCTC 1 cut(s) 29
MseI TTAA 1 cut(s) 141
MspI CCGG 1 cut(s) 60
MspR9I CCNGG 1 cut(s) 61
MwoI GCNNNNNNNGC 2 cut(s) 234, 463
NciI CCSGG 1 cut(s) 61
NdeII GATC 1 cut(s) 38
NlaIII CATG 3 cut(s) 174, 250, 435
NlaIV GGNNCC 2 cut(s) 40, 55
PfeI GAWTC 2 cut(s) 250, 451
PkrI GCNGC 2 cut(s) 227, 239
PleI GAGTC 2 cut(s) 89, 317
PpsI GAGTC 2 cut(s) 89, 317
PpuMI RGGWCCY 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 380
PspN4I GGNNCC 2 cut(s) 40, 55
PspPI GGNCC 3 cut(s) 54, 380, 440
PspPPI RGGWCCY 1 cut(s) 380
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 38
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 2 cut(s) 226, 238
Sau3AI GATC 1 cut(s) 38
Sau96I GGNCC 3 cut(s) 54, 380, 440
SchI GAGTC 2 cut(s) 89, 317
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 1 cut(s) 422
SfaNI GCATC 2 cut(s) 108, 306
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SpeI ACTAGT 1 cut(s) 302
Sse9I AATT 1 cut(s) 138
SsiI CCGC 1 cut(s) 113
SspI AATATT 1 cut(s) 334
SspMI CTAG 2 cut(s) 92, 303
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 67
TaqI TCGA 1 cut(s) 321
TasI AATT 1 cut(s) 138
TfiI GAWTC 2 cut(s) 250, 451
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 1 cut(s) 235
TseI GCWGC 2 cut(s) 225, 237
TspDTI ATGAA 1 cut(s) 263
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 380
XspI CTAG 2 cut(s) 92, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.