Rh5AG292600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0017356
Physical Location & Seq
Forward (+)
40498412 .. 40500419
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG292600.1

Sequence Viewer

Length: 1275 bp
ATGTATTCTGAAGATTACCCCGAGGCCGTCGGGTCCTCAACACCCGTAGTAGAGTTTGTGTCGGAATCAGAACTGTGGGAGGAGCAAGCAAACTGGGATGACCAACACAAAAGCTTATATTTGTGCGTGGGCGTCTACTATGGGAGAAGAGGCAAGGTACTGTATGTTGTTTATCGTATCAATTTGAAAGCCCTCTTGTTTAGTGAGGATCTAGAGTTTGAAACCGTGTTCGAAGTTCCCGATAATGATAATATTGGGAATTGTGCCGGATTTGGTATGGGACTTTCTATGATATTCTTGGGTGGAGGATTTAGAGCCAAGTACTCGAAAGAATTTGTTGTCCCAGAGCCGTGTACAGGTATTCTGGCCTATCAGATTCGTGAAATTTTTGAAGGTGGTCATTTAGCTCATTTCTTAGACGGCAAAGTGAGGCCACTTATATGGGAGGTTGAAGGAAATTTGTTCGCTCTCGCTCGTAACCCGCCTCCTGGAATTGACACCGGTTTCCCAGCTAGTGAATGGAGAAACTGTTTTGAAATGTATGACCGGGCCCAGGGTCGATGGATTGGTTTGCCGGACCCTCCTTCTTATGCTGAATACCATGTCGCTGCTGTTGATGAGTCTGGTGGTGGTGGTATTGATGAGGAGGAGGAGTTTGTTGGTTGTGATGACAGCGCTTTGAGAAGGCACTGTTACTATTCAAGCTTCTCTTATGCAATTTTAGGTAGCCAACTTTTCGTCTCTAATCCAAACTATCATGAATATTCATACAAGTGCAACCTTCCCAGCAAGCGTGAAAACTGGCCAGTTGAGTGGAAAGTTTCAAGTTTCCAATTTCAGGGTGTAACTCTAGGGGTTCAAACGGCACAAGGGTTTGTAGTCTTTTCCTATGATGTCAATTGCCGTCCGCCTGAATATGTGGTTAAACTTGAAGATAATCCCTACTTAAATATCAAAGTGCAACTGTTGATAAATGGCTATGATGAACCGGTGCTTTCTCAATCTCTAATGATGCCGCAGCAGCTGGATGAAGTTCAAGACTCCCAGTTTGTTCACCTAGGAGGTCAAAAAATCTGCTTTCTCTATTCTAGAATGAGAGATTCCAAGTTGCTTGTAACCGCCATCACATTTGGCTTCCAACTCGAACAGAGAAATAGCGGCTTCAAAGTCAAAGCCAAATTTTATCCTACTCGCAACTGGGAGTTCCAAAATAAGGCTGGGTCTAAGGTTAAAGTTTGGGGTACAGAACAGATGGTTGGTGCTGTTGTTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

424

Amino Acids

48.15

Weight (kDa)

4.91

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017356)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04490
rosa_chinensis RchiOBHm_Chr5g0043831
rosa_laevigata RLG00000034216
rosa_multiflora Rmu_co8126332.1_g000001 Rmu_sc0002448.1_g000002 Rmu_sc0006691.1_g000009 Rmu_sc0008441.1_g000016
rosa_roxburghii Rroxscaffold_1G00037220
rosa_rugosa Rorug05G0208500
rosa_samantha Rh5AG292600 Rh5BG300500 Rh5DG309500
rosa_wichuraiana Rw5G027120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AciI CCGC 5 cut(s) 482, 908, 1016, 1119, 1158
AclWI GGATC 1 cut(s) 216
AcoI YGGCCR 1 cut(s) 803
AcsI RAATTY 4 cut(s) 332, 384, 457, 1178
AcuI CTGAAG 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 4 cut(s) 159, 323, 355, 1243
AfeI AGCGCT 1 cut(s) 676
AfiI CCNNNNNNNGG 5 cut(s) 356, 488, 553, 838, 1213
AgeI ACCGGT 2 cut(s) 500, 988
AjnI CCWGG 2 cut(s) 487, 552
AjuI GAANNNNNNNTTGG 2 cut(s) 1239, 1271
AluBI AGCT 5 cut(s) 114, 407, 512, 705, 1024
AluI AGCT 5 cut(s) 114, 407, 512, 705, 1024
Alw26I GTCTC 1 cut(s) 745
AlwI GGATC 1 cut(s) 216
AlwNI CAGNNNCTG 1 cut(s) 1024
Ama87I CYCGRG 1 cut(s) 20
Aor51HI AGCGCT 1 cut(s) 676
AoxI GGCC 5 cut(s) 24, 366, 431, 549, 803
ApaI GGGCCC 1 cut(s) 553
ApeKI GCWGC 3 cut(s) 608, 1018, 1021
ApoI RAATTY 4 cut(s) 332, 384, 457, 1178
ArsI GACNNNNNNTTYG 2 cut(s) 1031, 1063
AsiGI ACCGGT 2 cut(s) 500, 988
AspA2I CCTAGG 1 cut(s) 1057
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 4 cut(s) 33, 549, 550, 577
AsuC2I CCSGG 1 cut(s) 548
AsuHPI GGTGA 1 cut(s) 1046
AsuII TTCGAA 1 cut(s) 231
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 2 cut(s) 33, 577
AvrII CCTAGG 1 cut(s) 1057
BaeGI GKGCMC 1 cut(s) 553
BalI TGGCCA 1 cut(s) 805
BanII GRGCYC 1 cut(s) 553
BbvI GCAGC 3 cut(s) 595, 1030, 1033
BccI CCATC 3 cut(s) 555, 1130, 1246
BceAI ACGGC 5 cut(s) 11, 334, 436, 879, 888
BciT130I CCWGG 2 cut(s) 489, 554
BcnI CCSGG 1 cut(s) 548
BcoDI GTCTC 1 cut(s) 745
BfaI CTAG 5 cut(s) 212, 513, 851, 1058, 1089
BfoI RGCGCY 1 cut(s) 678
BisI GCNGC 5 cut(s) 609, 1016, 1019, 1022, 1159
BlnI CCTAGG 1 cut(s) 1057
BlsI GCNGC 5 cut(s) 610, 1017, 1020, 1023, 1160
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 3 cut(s) 489, 548, 554
Bme18I GGWCC 2 cut(s) 33, 577
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 4 cut(s) 33, 549, 550, 577
BmiI GGNNCC 3 cut(s) 34, 551, 579
BmrFI CCNGG 3 cut(s) 489, 548, 554
BmrI ACTGGG 3 cut(s) 103, 1039, 1207
BmsI GCATC 1 cut(s) 1002
BmuI ACTGGG 3 cut(s) 103, 1039, 1207
Bpu14I TTCGAA 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 4 cut(s) 21, 552, 553, 1057
BsaWI WCCGGW 2 cut(s) 500, 988
BsaXI ACNNNNNCTCC 4 cut(s) 514, 544, 638, 668
Bsc4I CCNNNNNNNGG 5 cut(s) 356, 488, 553, 838, 1213
Bse118I RCCGGY 2 cut(s) 500, 988
Bse1I ACTGG 5 cut(s) 98, 806, 806, 1045, 1202
BseBI CCWGG 2 cut(s) 489, 554
BseDI CCNNGG 4 cut(s) 21, 552, 553, 1057
BseGI GGATG 2 cut(s) 103, 1033
BseLI CCNNNNNNNGG 5 cut(s) 356, 488, 553, 838, 1213
BseNI ACTGG 5 cut(s) 98, 806, 806, 1045, 1202
BseRI GAGGAG 4 cut(s) 95, 659, 662, 665
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 3 cut(s) 595, 1030, 1033
BseYI CCCAGC 3 cut(s) 508, 785, 1217
BshFI GGCC 5 cut(s) 26, 368, 433, 551, 805
BshTI ACCGGT 2 cut(s) 500, 988
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 5 cut(s) 267, 501, 547, 575, 989
BslFI GGGAC 2 cut(s) 294, 326
BslI CCNNNNNNNGG 5 cut(s) 356, 488, 553, 838, 1213
BsmAI GTCTC 1 cut(s) 745
BsmBI CGTCTC 1 cut(s) 745
BsmFI GGGAC 2 cut(s) 294, 326
BsnI GGCC 5 cut(s) 26, 368, 433, 551, 805
BsoBI CYCGRG 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 231
Bsp120I GGGCCC 1 cut(s) 549
Bsp1286I GDGCHC 1 cut(s) 553
Bsp1407I TGTACA 1 cut(s) 353
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 5 cut(s) 482, 908, 1016, 1119, 1158
BspANI GGCC 5 cut(s) 26, 368, 433, 551, 805
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 3 cut(s) 34, 551, 579
BspPI GGATC 1 cut(s) 216
BspT104I TTCGAA 1 cut(s) 231
BsrFI RCCGGY 2 cut(s) 500, 988
BsrGI TGTACA 1 cut(s) 353
BsrI ACTGG 5 cut(s) 98, 806, 806, 1045, 1202
BssAI RCCGGY 2 cut(s) 500, 988
BssECI CCNNGG 4 cut(s) 21, 552, 553, 1057
BssMI GATC 1 cut(s) 208
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 1057
Bst2UI CCWGG 2 cut(s) 489, 554
Bst4CI ACNGT 6 cut(s) 75, 162, 226, 530, 692, 966
Bst6I CTCTTC 1 cut(s) 142
BstACI GRCGYC 1 cut(s) 132
BstAUI TGTACA 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 87, 791
BstDEI CTNAG 2 cut(s) 415, 1224
BstF5I GGATG 2 cut(s) 103, 1033
BstH2I RGCGCY 1 cut(s) 678
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 1 cut(s) 211
BstMAI GTCTC 1 cut(s) 745
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 1 cut(s) 1021
BstNI CCWGG 2 cut(s) 489, 554
BstSCI CCNGG 3 cut(s) 487, 546, 552
BstSLI GKGCMC 1 cut(s) 553
BstV1I GCAGC 3 cut(s) 595, 1030, 1033
BstX2I RGATCY 1 cut(s) 208
BstXI CCANNNNNNTGG 2 cut(s) 441, 813
BstYI RGATCY 1 cut(s) 208
BsuRI GGCC 5 cut(s) 26, 368, 433, 551, 805
BtsCI GGATG 2 cut(s) 103, 1033
BtsIMutI CAGTG 1 cut(s) 688
Cac8I GCNNGC 2 cut(s) 87, 791
CaiI CAGNNNCTG 1 cut(s) 1024
CciI TCATGA 1 cut(s) 757
CfoI GCGC 1 cut(s) 677
Cfr10I RCCGGY 2 cut(s) 500, 988
Cfr13I GGNCC 4 cut(s) 33, 549, 550, 577
CseI GACGC 1 cut(s) 121
Csp6I GTAC 4 cut(s) 158, 322, 354, 1242
CspAI ACCGGT 2 cut(s) 500, 988
CviAII CATG 2 cut(s) 602, 758
CviQI GTAC 4 cut(s) 158, 322, 354, 1242
DdeI CTNAG 2 cut(s) 415, 1224
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 803
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
EciI GGCGGA 1 cut(s) 897
Eco130I CCWWGG 1 cut(s) 1057
Eco24I GRGCYC 1 cut(s) 553
Eco47I GGWCC 2 cut(s) 33, 577
Eco47III AGCGCT 1 cut(s) 676
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 33
EcoRII CCWGG 2 cut(s) 487, 552
EcoT14I CCWWGG 1 cut(s) 1057
EcoT38I GRGCYC 1 cut(s) 553
ErhI CCWWGG 1 cut(s) 1057
Esp3I CGTCTC 1 cut(s) 745
FaeI CATG 2 cut(s) 605, 761
FalI AAGNNNNNCTT 2 cut(s) 694, 726
FaqI GGGAC 2 cut(s) 294, 326
FatI CATG 2 cut(s) 601, 757
FauI CCCGC 1 cut(s) 489
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 609, 1016, 1019, 1022, 1159
FokI GGATG 2 cut(s) 110, 1040
FriOI GRGCYC 1 cut(s) 553
Fsp4HI GCNGC 5 cut(s) 609, 1016, 1019, 1022, 1159
FspBI CTAG 5 cut(s) 212, 513, 851, 1058, 1089
GlaI GCGC 1 cut(s) 676
GluI GCNGC 5 cut(s) 609, 1016, 1019, 1022, 1159
GsaI CCCAGC 3 cut(s) 512, 789, 1221
HaeII RGCGCY 1 cut(s) 678
HaeIII GGCC 5 cut(s) 26, 368, 433, 551, 805
HapII CCGG 5 cut(s) 267, 501, 547, 575, 989
HgaI GACGC 1 cut(s) 121
HhaI GCGC 1 cut(s) 677
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 2 cut(s) 605, 761
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HindIII AAGCTT 2 cut(s) 112, 703
HinfI GANTC 5 cut(s) 65, 376, 620, 1040, 1100
HpaII CCGG 5 cut(s) 267, 501, 547, 575, 989
HphI GGTGA 1 cut(s) 1046
Hpy166II GTNNAC 3 cut(s) 136, 354, 1054
Hpy188I TCNGA 4 cut(s) 10, 64, 70, 375
Hpy188III TCNNGA 6 cut(s) 212, 239, 380, 758, 1037, 1089
Hpy8I GTNNAC 3 cut(s) 136, 354, 1054
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 5 cut(s) 386, 446, 594, 678, 791
HpyCH4III ACNGT 6 cut(s) 75, 162, 226, 530, 692, 966
HpyCH4V TGCA 3 cut(s) 716, 777, 961
HpyF10VI GCNNNNNNNGC 1 cut(s) 1021
HpyF3I CTNAG 2 cut(s) 415, 1224
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 2 cut(s) 605, 761
HspAI GCGC 1 cut(s) 675
Kzo9I GATC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 3 cut(s) 595, 1030, 1033
LweI GCATC 1 cut(s) 1002
MaeI CTAG 5 cut(s) 212, 513, 851, 1058, 1089
MaeIII GTNAC 4 cut(s) 476, 692, 844, 1114
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 3 cut(s) 23, 159, 944
MfeI CAATTG 1 cut(s) 898
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 553
MlsI TGGCCA 1 cut(s) 805
MluNI TGGCCA 1 cut(s) 805
MlyI GAGTC 2 cut(s) 629, 1034
MmeI TCCRAC 2 cut(s) 42, 1162
Mox20I TGGCCA 1 cut(s) 805
MscI TGGCCA 1 cut(s) 805
MseI TTAA 3 cut(s) 924, 947, 1230
MslI CAYNNNNRTG 2 cut(s) 439, 772
Msp20I TGGCCA 1 cut(s) 805
MspA1I CMGCKG 1 cut(s) 1024
MspI CCGG 5 cut(s) 267, 501, 547, 575, 989
MspR9I CCNGG 3 cut(s) 489, 548, 554
MunI CAATTG 1 cut(s) 898
MvaI CCWGG 2 cut(s) 489, 554
MwoI GCNNNNNNNGC 1 cut(s) 1021
NciI CCSGG 1 cut(s) 548
NdeII GATC 1 cut(s) 208
NlaIII CATG 2 cut(s) 605, 761
NlaIV GGNNCC 3 cut(s) 34, 551, 579
NspV TTCGAA 1 cut(s) 231
PagI TCATGA 1 cut(s) 757
PasI CCCWGGG 1 cut(s) 553
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 3 cut(s) 65, 376, 1100
PfoI TCCNGGA 1 cut(s) 487
PinAI ACCGGT 2 cut(s) 500, 988
PkrI GCNGC 5 cut(s) 610, 1017, 1020, 1023, 1160
PleI GAGTC 2 cut(s) 628, 1034
PpsI GAGTC 2 cut(s) 628, 1034
PpuMI RGGWCCY 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 2 cut(s) 487, 552
PspFI CCCAGC 3 cut(s) 508, 785, 1217
PspGI CCWGG 2 cut(s) 487, 552
PspN4I GGNNCC 3 cut(s) 34, 551, 579
PspOMI GGGCCC 1 cut(s) 549
PspPI GGNCC 4 cut(s) 33, 549, 550, 577
PspPPI RGGWCCY 1 cut(s) 33
PstNI CAGNNNCTG 1 cut(s) 1024
PsuI RGATCY 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 1024
RsaI GTAC 4 cut(s) 159, 323, 355, 1243
RsaNI GTAC 4 cut(s) 158, 322, 354, 1242
RseI CAYNNNNRTG 2 cut(s) 439, 772
SaqAI TTAA 3 cut(s) 924, 947, 1230
SatI GCNGC 5 cut(s) 609, 1016, 1019, 1022, 1159
Sau3AI GATC 1 cut(s) 208
Sau96I GGNCC 4 cut(s) 33, 549, 550, 577
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 2 cut(s) 629, 1034
ScrFI CCNGG 3 cut(s) 489, 548, 554
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 1 cut(s) 1002
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 2 cut(s) 33, 577
SmiMI CAYNNNNRTG 2 cut(s) 439, 772
SsiI CCGC 5 cut(s) 482, 908, 1016, 1119, 1158
SspI AATATT 2 cut(s) 253, 764
SspMI CTAG 5 cut(s) 212, 513, 851, 1058, 1089
StyD4I CCNGG 3 cut(s) 487, 546, 552
StyI CCWWGG 1 cut(s) 1057
TaaI ACNGT 6 cut(s) 75, 162, 226, 530, 692, 966
TaqI TCGA 4 cut(s) 231, 326, 559, 1143
TatI WGTACW 2 cut(s) 321, 353
TauI GCSGC 2 cut(s) 1018, 1161
TfiI GAWTC 3 cut(s) 65, 376, 1100
Tru1I TTAA 3 cut(s) 924, 947, 1230
Tru9I TTAA 3 cut(s) 924, 947, 1230
TscAI CASTG 1 cut(s) 695
TseI GCWGC 3 cut(s) 608, 1018, 1021
TspDTI ATGAA 4 cut(s) 756, 774, 999, 1044
TspRI CASTG 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 33, 577
XapI RAATTY 4 cut(s) 332, 384, 457, 1178
XbaI TCTAGA 2 cut(s) 211, 1088
XcmI CCANNNNNNNNNTGG 2 cut(s) 516, 1214
XmaJI CCTAGG 1 cut(s) 1057
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 5 cut(s) 212, 513, 851, 1058, 1089
ZrmI AGTACT 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.