Rw5G027120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0017356
Physical Location & Seq
Forward (+)
39833947 .. 39835931
1985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027120.1

Sequence Viewer

Length: 1290 bp
ATGTATTCTGAAGATTACCCTGAGGCCGTTGGGTCCTCAACACCGGCAGTAGAGTTTGTGTCGGAATCAGAACTGTGGGAGGAGCAAGCAAACTGGGATGACCAAGACAAAAGCTTATATTTGTGCGTGGGCGACTACTATGGGAGAAGAGGCAAGGTACTGTATGTTGTTTATCGTATCAATTTGAAAGCCCTCTTGTTTAGTGAGGATCTAGAGTTTGAAACCTTGTTCGAAGTTCCCGATAATGATAATATTGGGAATTGTGCCGGATTTGGTATGGGACTTTCTATGATATTCTTGGGTGGAGGATTTAGAGCCAAGTACTCGAAAGAATTTGTTGTCCCAGAGCCGTGTACAGGTATTCTGGCCTATCAGATTCGTGAAATTTTTGAAGGTGGTCGTTTAGCTCATTTCTTAGACGGCAAAGTGAGGCCACTTATATGGGAGGTTGAAGGAAATTTGTTCGCTCTCGCTCGTAACCCGCCTCCTGGAATTGACACCGGTTTCCCAGTTAGTGAATGGAGAAACTGTTTTGAAATGTATGACCGGGCCCAGGGTCGATGGGTTGGTTTGCCGGACCCTCCTTCTTATGCTGAATACCCTGTCGCTGCTGTTGATGTGTCTGGTGGTGGTGGTATTGATGAGGGTGGTGATGAGGAGGAGGAGGAGTTCGTTGGTTGTGATGACGGCGGTTTGAGAAGGCACTGTTACTATTCAAGCTTCTCTTATGCAATTTTAGGCAGCCAACTTTTCGTGTCTAATCCACAATATCATGAATATTCATACAAGTGCAACCTTCCCAGCAAGCGTGAAAACTGGCCAGTTGAGTGGAAAGTTTCAAGTTTCCAATTTCAGGGTGTAACTCTAGGGGTTCAAACGGCACAAGGGTTTGTAGTCTTTTCCTATGATGTCAATTGCCATCCGCCTGAATATGTGGTTAAACTTGAAGATAATCCCTACTTAAATATCAAAGTGCAACTGTTGATAAATGGCTATGATGAACCGGTGCTTTCTCAATCTCTAATGATGCCGCAACAGCTGGATCAAGTTCAAGACTCCCAGTTTGTTCACCTAGGAGGTCAAAAAGTCTGCTTTCTCTATTCTAGAATGAGAAATTCCAAGTTGCTTGTAACCGCCATCACATTTGGCTTCCAACTCGAACAGAGAAACAGCGGCTTCAAAGTCAAAGCCAAATTTTATCCTACTCGCAACTGGGAATTCCAAAATAAGGCTGGGTCTAAGGCTAAAGTTTGGGGTACGGAACAGATGGTTGGTGCTGTTGTTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

429

Amino Acids

48.49

Weight (kDa)

4.75

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017356)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04490
rosa_chinensis RchiOBHm_Chr5g0043831
rosa_laevigata RLG00000034216
rosa_multiflora Rmu_co8126332.1_g000001 Rmu_sc0002448.1_g000002 Rmu_sc0006691.1_g000009 Rmu_sc0008441.1_g000016
rosa_roxburghii Rroxscaffold_1G00037220
rosa_rugosa Rorug05G0208500
rosa_samantha Rh5AG292600 Rh5BG300500 Rh5DG309500
rosa_wichuraiana Rw5G027120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 482, 690, 923, 1031, 1134, 1173
AclWI GGATC 2 cut(s) 216, 1050
AcoI YGGCCR 1 cut(s) 818
AcsI RAATTY 6 cut(s) 332, 384, 457, 1114, 1193, 1217
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 4 cut(s) 159, 323, 355, 1258
AfiI CCNNNNNNNGG 5 cut(s) 356, 488, 553, 853, 1228
AgeI ACCGGT 2 cut(s) 500, 1003
AjnI CCWGG 2 cut(s) 487, 552
AjuI GAANNNNNNNTTGG 2 cut(s) 1254, 1286
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 4 cut(s) 114, 407, 720, 1039
AluI AGCT 4 cut(s) 114, 407, 720, 1039
AlwI GGATC 2 cut(s) 216, 1050
AoxI GGCC 5 cut(s) 24, 366, 431, 549, 818
ApaI GGGCCC 1 cut(s) 553
ApeKI GCWGC 2 cut(s) 608, 741
ApoI RAATTY 6 cut(s) 332, 384, 457, 1114, 1193, 1217
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
AsiGI ACCGGT 2 cut(s) 500, 1003
AspA2I CCTAGG 1 cut(s) 1072
AspS9I GGNCC 4 cut(s) 33, 549, 550, 577
AsuC2I CCSGG 1 cut(s) 548
AsuHPI GGTGA 2 cut(s) 662, 1061
AsuII TTCGAA 1 cut(s) 231
AvaII GGWCC 2 cut(s) 33, 577
AvrII CCTAGG 1 cut(s) 1072
AxyI CCTNAGG 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 553
BalI TGGCCA 1 cut(s) 820
BanII GRGCYC 1 cut(s) 553
BbvI GCAGC 2 cut(s) 595, 753
BccI CCATC 4 cut(s) 555, 927, 1145, 1261
BceAI ACGGC 5 cut(s) 11, 334, 436, 703, 894
BciT130I CCWGG 2 cut(s) 489, 554
BcnI CCSGG 1 cut(s) 548
BfaI CTAG 4 cut(s) 212, 866, 1073, 1104
BisI GCNGC 4 cut(s) 609, 742, 1031, 1174
BlnI CCTAGG 1 cut(s) 1072
BlsI GCNGC 4 cut(s) 610, 743, 1032, 1175
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 3 cut(s) 489, 548, 554
Bme18I GGWCC 2 cut(s) 33, 577
BmgT120I GGNCC 4 cut(s) 33, 549, 550, 577
BmiI GGNNCC 3 cut(s) 34, 551, 579
BmrFI CCNGG 3 cut(s) 489, 548, 554
BmrI ACTGGG 4 cut(s) 103, 503, 1054, 1222
BmsI GCATC 1 cut(s) 1017
BmuI ACTGGG 4 cut(s) 103, 503, 1054, 1222
Bpu14I TTCGAA 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 548
BsaJI CCNNGG 3 cut(s) 552, 553, 1072
BsaWI WCCGGW 2 cut(s) 500, 1003
BsaXI ACNNNNNCTCC 4 cut(s) 514, 544, 653, 683
Bsc4I CCNNNNNNNGG 5 cut(s) 356, 488, 553, 853, 1228
Bse118I RCCGGY 3 cut(s) 43, 500, 1003
Bse1I ACTGG 6 cut(s) 98, 509, 821, 821, 1060, 1217
Bse21I CCTNAGG 1 cut(s) 21
BseBI CCWGG 2 cut(s) 489, 554
BseDI CCNNGG 3 cut(s) 552, 553, 1072
BseGI GGATG 2 cut(s) 103, 919
BseLI CCNNNNNNNGG 5 cut(s) 356, 488, 553, 853, 1228
BseMII CTCAG 1 cut(s) 12
BseNI ACTGG 6 cut(s) 98, 509, 821, 821, 1060, 1217
BseRI GAGGAG 5 cut(s) 95, 671, 674, 677, 680
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 2 cut(s) 595, 753
BseYI CCCAGC 2 cut(s) 800, 1232
BshFI GGCC 5 cut(s) 26, 368, 433, 551, 820
BshTI ACCGGT 2 cut(s) 500, 1003
BsiSI CCGG 6 cut(s) 44, 267, 501, 547, 575, 1004
BslFI GGGAC 2 cut(s) 294, 326
BslI CCNNNNNNNGG 5 cut(s) 356, 488, 553, 853, 1228
BsmFI GGGAC 2 cut(s) 294, 326
BsnI GGCC 5 cut(s) 26, 368, 433, 551, 820
Bsp119I TTCGAA 1 cut(s) 231
Bsp120I GGGCCC 1 cut(s) 549
Bsp1286I GDGCHC 1 cut(s) 553
Bsp1407I TGTACA 1 cut(s) 353
Bsp143I GATC 2 cut(s) 208, 1042
BspACI CCGC 6 cut(s) 482, 690, 923, 1031, 1134, 1173
BspANI GGCC 5 cut(s) 26, 368, 433, 551, 820
BspCNI CTCAG 1 cut(s) 13
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 3 cut(s) 34, 551, 579
BspPI GGATC 2 cut(s) 216, 1050
BspT104I TTCGAA 1 cut(s) 231
BsrFI RCCGGY 3 cut(s) 43, 500, 1003
BsrGI TGTACA 1 cut(s) 353
BsrI ACTGG 6 cut(s) 98, 509, 821, 821, 1060, 1217
BssAI RCCGGY 3 cut(s) 43, 500, 1003
BssECI CCNNGG 3 cut(s) 552, 553, 1072
BssMI GATC 2 cut(s) 208, 1042
BssT1I CCWWGG 1 cut(s) 1072
Bst2UI CCWGG 2 cut(s) 489, 554
Bst4CI ACNGT 5 cut(s) 75, 162, 530, 707, 981
Bst6I CTCTTC 1 cut(s) 142
BstAUI TGTACA 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 87, 806
BstDEI CTNAG 3 cut(s) 21, 415, 1239
BstF5I GGATG 2 cut(s) 103, 919
BstKTI GATC 2 cut(s) 211, 1045
BstMBI GATC 2 cut(s) 208, 1042
BstMWI GCNNNNNNNGC 1 cut(s) 1036
BstNI CCWGG 2 cut(s) 489, 554
BstSCI CCNGG 3 cut(s) 487, 546, 552
BstSLI GKGCMC 1 cut(s) 553
BstV1I GCAGC 2 cut(s) 595, 753
BstX2I RGATCY 1 cut(s) 208
BstXI CCANNNNNNTGG 2 cut(s) 441, 828
BstYI RGATCY 1 cut(s) 208
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 5 cut(s) 26, 368, 433, 551, 820
BtsCI GGATG 2 cut(s) 103, 919
BtsIMutI CAGTG 1 cut(s) 703
Cac8I GCNNGC 2 cut(s) 87, 806
CciI TCATGA 1 cut(s) 772
Cfr10I RCCGGY 3 cut(s) 43, 500, 1003
Cfr13I GGNCC 4 cut(s) 33, 549, 550, 577
Csp6I GTAC 4 cut(s) 158, 322, 354, 1257
CspAI ACCGGT 2 cut(s) 500, 1003
CviAII CATG 1 cut(s) 773
CviQI GTAC 4 cut(s) 158, 322, 354, 1257
DdeI CTNAG 3 cut(s) 21, 415, 1239
DpnI GATC 2 cut(s) 210, 1044
DpnII GATC 2 cut(s) 208, 1042
EaeI YGGCCR 1 cut(s) 818
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
EciI GGCGGA 1 cut(s) 912
Eco130I CCWWGG 1 cut(s) 1072
Eco24I GRGCYC 1 cut(s) 553
Eco47I GGWCC 2 cut(s) 33, 577
Eco57I CTGAAG 1 cut(s) 30
Eco81I CCTNAGG 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 33
EcoRI GAATTC 1 cut(s) 1217
EcoRII CCWGG 2 cut(s) 487, 552
EcoT14I CCWWGG 1 cut(s) 1072
EcoT38I GRGCYC 1 cut(s) 553
ErhI CCWWGG 1 cut(s) 1072
FaeI CATG 1 cut(s) 776
FalI AAGNNNNNCTT 2 cut(s) 709, 741
FaqI GGGAC 2 cut(s) 294, 326
FatI CATG 1 cut(s) 772
FauI CCCGC 1 cut(s) 489
Fnu4HI GCNGC 4 cut(s) 609, 742, 1031, 1174
FokI GGATG 2 cut(s) 110, 906
FriOI GRGCYC 1 cut(s) 553
Fsp4HI GCNGC 4 cut(s) 609, 742, 1031, 1174
FspBI CTAG 4 cut(s) 212, 866, 1073, 1104
GluI GCNGC 4 cut(s) 609, 742, 1031, 1174
GsaI CCCAGC 2 cut(s) 804, 1236
HaeIII GGCC 5 cut(s) 26, 368, 433, 551, 820
HapII CCGG 6 cut(s) 44, 267, 501, 547, 575, 1004
Hin1II CATG 1 cut(s) 776
HindIII AAGCTT 2 cut(s) 112, 718
HinfI GANTC 3 cut(s) 65, 376, 1055
HpaII CCGG 6 cut(s) 44, 267, 501, 547, 575, 1004
HphI GGTGA 2 cut(s) 662, 1061
Hpy166II GTNNAC 2 cut(s) 354, 1069
Hpy188I TCNGA 4 cut(s) 10, 64, 70, 375
Hpy188III TCNNGA 6 cut(s) 212, 239, 380, 773, 1052, 1104
Hpy8I GTNNAC 2 cut(s) 354, 1069
HpyAV CCTTC 5 cut(s) 386, 446, 594, 693, 806
HpyCH4III ACNGT 5 cut(s) 75, 162, 530, 707, 981
HpyCH4V TGCA 3 cut(s) 731, 792, 976
HpyF10VI GCNNNNNNNGC 1 cut(s) 1036
HpyF3I CTNAG 3 cut(s) 21, 415, 1239
Hsp92II CATG 1 cut(s) 776
Kzo9I GATC 2 cut(s) 208, 1042
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 2 cut(s) 595, 753
LweI GCATC 1 cut(s) 1017
MaeI CTAG 4 cut(s) 212, 866, 1073, 1104
MaeIII GTNAC 4 cut(s) 476, 707, 859, 1129
MalI GATC 2 cut(s) 210, 1044
MboI GATC 2 cut(s) 208, 1042
MboII GAAGA 3 cut(s) 23, 159, 959
MfeI CAATTG 1 cut(s) 913
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 553
MlsI TGGCCA 1 cut(s) 820
MluNI TGGCCA 1 cut(s) 820
MlyI GAGTC 1 cut(s) 1049
MmeI TCCRAC 2 cut(s) 42, 1177
Mox20I TGGCCA 1 cut(s) 820
MscI TGGCCA 1 cut(s) 820
MseI TTAA 2 cut(s) 939, 962
MslI CAYNNNNRTG 2 cut(s) 439, 787
Msp20I TGGCCA 1 cut(s) 820
MspA1I CMGCKG 2 cut(s) 1039, 1173
MspI CCGG 6 cut(s) 44, 267, 501, 547, 575, 1004
MspR9I CCNGG 3 cut(s) 489, 548, 554
MunI CAATTG 1 cut(s) 913
MvaI CCWGG 2 cut(s) 489, 554
MwoI GCNNNNNNNGC 1 cut(s) 1036
NciI CCSGG 1 cut(s) 548
NdeII GATC 2 cut(s) 208, 1042
NlaIII CATG 1 cut(s) 776
NlaIV GGNNCC 3 cut(s) 34, 551, 579
NspV TTCGAA 1 cut(s) 231
PagI TCATGA 1 cut(s) 772
PasI CCCWGGG 1 cut(s) 553
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 2 cut(s) 65, 376
PfoI TCCNGGA 1 cut(s) 487
PinAI ACCGGT 2 cut(s) 500, 1003
PkrI GCNGC 4 cut(s) 610, 743, 1032, 1175
PleI GAGTC 1 cut(s) 1049
PpsI GAGTC 1 cut(s) 1049
PpuMI RGGWCCY 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 2 cut(s) 487, 552
PspFI CCCAGC 2 cut(s) 800, 1232
PspGI CCWGG 2 cut(s) 487, 552
PspN4I GGNNCC 3 cut(s) 34, 551, 579
PspOMI GGGCCC 1 cut(s) 549
PspPI GGNCC 4 cut(s) 33, 549, 550, 577
PspPPI RGGWCCY 1 cut(s) 33
PsuI RGATCY 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 1039
RsaI GTAC 4 cut(s) 159, 323, 355, 1258
RsaNI GTAC 4 cut(s) 158, 322, 354, 1257
RseI CAYNNNNRTG 2 cut(s) 439, 787
SaqAI TTAA 2 cut(s) 939, 962
SatI GCNGC 4 cut(s) 609, 742, 1031, 1174
Sau3AI GATC 2 cut(s) 208, 1042
Sau96I GGNCC 4 cut(s) 33, 549, 550, 577
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 1 cut(s) 1049
ScrFI CCNGG 3 cut(s) 489, 548, 554
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 1 cut(s) 1017
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 2 cut(s) 33, 577
SmiMI CAYNNNNRTG 2 cut(s) 439, 787
SsiI CCGC 6 cut(s) 482, 690, 923, 1031, 1134, 1173
SspI AATATT 2 cut(s) 253, 779
SspMI CTAG 4 cut(s) 212, 866, 1073, 1104
StyD4I CCNGG 3 cut(s) 487, 546, 552
StyI CCWWGG 1 cut(s) 1072
TaaI ACNGT 5 cut(s) 75, 162, 530, 707, 981
TaqI TCGA 4 cut(s) 231, 326, 559, 1158
TatI WGTACW 2 cut(s) 321, 353
TauI GCSGC 2 cut(s) 1033, 1176
TfiI GAWTC 2 cut(s) 65, 376
Tru1I TTAA 2 cut(s) 939, 962
Tru9I TTAA 2 cut(s) 939, 962
TscAI CASTG 1 cut(s) 710
TseI GCWGC 2 cut(s) 608, 741
TspDTI ATGAA 3 cut(s) 771, 789, 1014
TspGWI ACGGA 1 cut(s) 1274
TspRI CASTG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 33, 577
XapI RAATTY 6 cut(s) 332, 384, 457, 1114, 1193, 1217
XbaI TCTAGA 2 cut(s) 211, 1103
XcmI CCANNNNNNNNNTGG 2 cut(s) 516, 1229
XmaJI CCTAGG 1 cut(s) 1072
XspI CTAG 4 cut(s) 212, 866, 1073, 1104
ZrmI AGTACT 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.