Rh5BG240500

TIR domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017096
Physical Location & Seq
Forward (+)
28814930 .. 28837825
22896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG240500.1

Sequence Viewer

Length: 513 bp
ATGCATCGTTCAACAGCTACTAGCTTCCAACGCTACCTTTTCAGCCATCAAAACCAAATCCAGTTGGCCAAACACGTTACTAAACCCTGTGATGTTTTCCTCAATCACAGAGGGATAGACACAAAGCGAACAATAGTATCTTTGCTTTACGACCATCTTTCTCGACTAAACCTGCGGCCTTTCTTGGACAACAAGAATATGAAACCTGGAGACAAACTGTTTGATAAGATTGATAGTGCAATACGGAGTTGCAAGGTCGGTGTTGCTGTGTTCTCGCCGCGCTATTGTGATTCTTACTTTTGCCTTCATGAGCTAGCTATGTTCATGGAGTCCAATAAGAAGGTGATTCCTATATTCTGTGACATTAAGCCCTCTCAGCTTCGTGTCGTGGACAATGGGCAATGTGATGTGGAGAAGCTGAGAAGGTTTAGGTCGGCTCTTGAACAGGCTAAAAGCACCGCAGGTCTGACGTTCAACTCCTCAGAAGGGGTCACCTTGAGGGGTGTGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIR TIR_2

Protein Analysis

170

Amino Acids

19.47

Weight (kDa)

9.53

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 31 - 128 6.8e-19 TIR domain
TIR_2 PF13676 32 - 131 2.4e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017096)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52900
fragaria_vesca FvH4_3g20660
prunus_persica Prupe.4G186200_v2.0.a1
pyrus_communis pycom11g21280
rosa_laevigata RLG00000033567
rosa_multiflora Rmu_sc0002460.1_g000081
rosa_roxburghii Rroxscaffold_1G00045640
rosa_rugosa Rorug05G0147600
rosa_samantha Rh5AG239800 Rh5BG240500 Rh5CG270900 Rh5DG248700
rosa_wichuraiana Rw5G021960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 180, 452
AccII CGCG 1 cut(s) 280
AciI CCGC 3 cut(s) 175, 278, 459
AcoI YGGCCR 1 cut(s) 66
AfiI CCNNNNNNNGG 1 cut(s) 486
AflIII ACRYGT 1 cut(s) 73
AgsI TTSAA 3 cut(s) 12, 443, 475
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 6 cut(s) 17, 24, 313, 317, 379, 418
AluI AGCT 6 cut(s) 17, 24, 313, 317, 379, 418
Alw26I GTCTC 1 cut(s) 204
AoxI GGCC 2 cut(s) 66, 176
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
AspLEI GCGC 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 355, 484
AsuNHI GCTAGC 1 cut(s) 313
BaeI ACNNNNGTAYC 2 cut(s) 120, 153
BalI TGGCCA 1 cut(s) 68
BccI CCATC 2 cut(s) 54, 162
BciT130I CCWGG 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 3 cut(s) 21, 314, 511
BfuAI ACCTGC 2 cut(s) 180, 452
BisI GCNGC 2 cut(s) 176, 278
BlsI GCNGC 2 cut(s) 177, 279
Bme1390I CCNGG 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 1 cut(s) 13
BmtI GCTAGC 1 cut(s) 317
BpmI CTGGAG 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 486
Bse1I ACTGG 1 cut(s) 61
Bse3DI GCAATG 1 cut(s) 407
BseBI CCWGG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 486
BseMI GCAATG 1 cut(s) 407
BseMII CTCAG 3 cut(s) 389, 410, 495
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 1 cut(s) 469
Bsh1236I CGCG 1 cut(s) 280
BshFI GGCC 2 cut(s) 68, 178
BslI CCNNNNNNNGG 1 cut(s) 486
BsmAI GTCTC 1 cut(s) 204
BsnI GGCC 2 cut(s) 68, 178
Bsp143I GATC 1 cut(s) 507
BspACI CCGC 3 cut(s) 175, 278, 459
BspANI GGCC 2 cut(s) 68, 178
BspCNI CTCAG 3 cut(s) 388, 411, 494
BspFNI CGCG 1 cut(s) 280
BspHI TCATGA 1 cut(s) 307
BspMI ACCTGC 2 cut(s) 180, 452
BspOI GCTAGC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 407
BsrI ACTGG 1 cut(s) 61
BssMI GATC 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 3 cut(s) 375, 419, 481
BstEII GGTNACC 1 cut(s) 490
BstFNI CGCG 1 cut(s) 280
BstHHI GCGC 1 cut(s) 282
BstKTI GATC 1 cut(s) 510
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 1 cut(s) 507
BstMWI GCNNNNNNNGC 2 cut(s) 30, 376
BstNI CCWGG 1 cut(s) 207
BstPI GGTNACC 1 cut(s) 490
BstSCI CCNGG 1 cut(s) 205
BstUI CGCG 1 cut(s) 280
BsuRI GGCC 2 cut(s) 68, 178
BveI ACCTGC 2 cut(s) 180, 452
Cac8I GCNNGC 1 cut(s) 315
CciI TCATGA 1 cut(s) 307
CfoI GCGC 1 cut(s) 282
CviAII CATG 2 cut(s) 308, 325
DdeI CTNAG 3 cut(s) 375, 419, 481
DpnI GATC 1 cut(s) 509
DpnII GATC 1 cut(s) 507
EaeI YGGCCR 1 cut(s) 66
Eco91I GGTNACC 1 cut(s) 490
EcoO65I GGTNACC 1 cut(s) 490
EcoRII CCWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 311, 328
FaiI YATR 5 cut(s) 200, 309, 320, 326, 353
FatI CATG 2 cut(s) 307, 324
Fnu4HI GCNGC 2 cut(s) 176, 278
Fsp4HI GCNGC 2 cut(s) 176, 278
FspBI CTAG 3 cut(s) 21, 314, 511
GlaI GCGC 1 cut(s) 281
GluI GCNGC 2 cut(s) 176, 278
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 2 cut(s) 68, 178
HhaI GCGC 1 cut(s) 282
Hin1II CATG 2 cut(s) 311, 328
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HinfI GANTC 3 cut(s) 290, 329, 346
HphI GGTGA 2 cut(s) 355, 484
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 2 cut(s) 468, 484
Hpy188III TCNNGA 3 cut(s) 162, 308, 440
Hpy8I GTNNAC 1 cut(s) 391
HpyAV CCTTC 4 cut(s) 314, 334, 417, 479
HpyCH4III ACNGT 1 cut(s) 219
HpyCH4IV ACGT 2 cut(s) 75, 470
HpyCH4V TGCA 3 cut(s) 4, 239, 252
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 376
HpyF3I CTNAG 3 cut(s) 375, 419, 481
HpySE526I ACGT 2 cut(s) 75, 470
Hsp92II CATG 2 cut(s) 311, 328
HspAI GCGC 1 cut(s) 280
Kzo9I GATC 1 cut(s) 507
LpnPI CCDG 7 cut(s) 74, 100, 185, 192, 219, 431, 447
LweI GCATC 1 cut(s) 13
MaeI CTAG 3 cut(s) 21, 314, 511
MaeII ACGT 2 cut(s) 75, 470
MaeIII GTNAC 3 cut(s) 76, 359, 490
MalI GATC 1 cut(s) 509
MboI GATC 1 cut(s) 507
MlsI TGGCCA 1 cut(s) 68
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 1 cut(s) 338
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 5 cut(s) 104, 110, 382, 490, 492
Mox20I TGGCCA 1 cut(s) 68
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 68
MseI TTAA 1 cut(s) 366
Msp20I TGGCCA 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 280
MwoI GCNNNNNNNGC 2 cut(s) 30, 376
NdeII GATC 1 cut(s) 507
NheI GCTAGC 1 cut(s) 313
NlaIII CATG 2 cut(s) 311, 328
NmuCI GTSAC 2 cut(s) 359, 490
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 307
PfeI GAWTC 2 cut(s) 290, 346
PkrI GCNGC 2 cut(s) 177, 279
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 205
PspEI GGTNACC 1 cut(s) 490
PspGI CCWGG 1 cut(s) 205
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 2 cut(s) 176, 278
Sau3AI GATC 1 cut(s) 507
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 207
SfaNI GCATC 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 496
SmoI CTYRAG 1 cut(s) 496
SsiI CCGC 3 cut(s) 175, 278, 459
SspMI CTAG 3 cut(s) 21, 314, 511
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 219
TaiI ACGT 2 cut(s) 78, 473
TaqI TCGA 1 cut(s) 163
TauI GCSGC 2 cut(s) 178, 280
TfiI GAWTC 2 cut(s) 290, 346
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TseFI GTSAC 2 cut(s) 359, 490
Tsp45I GTSAC 2 cut(s) 359, 490
TspDTI ATGAA 3 cut(s) 215, 296, 313
TspGWI ACGGA 1 cut(s) 259
XspI CTAG 3 cut(s) 21, 314, 511
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.