Rh5BG425400

5'-methylthioadenosine S-adenosylhomocysteine nucleosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018684
Physical Location & Seq
Reverse (-)
68894935 .. 68899189
4255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG425400.1

Sequence Viewer

Length: 198 bp
ATGGCTCCTCACGGCGACAAATCGGACGCCGCGGAGGACGCCATGGTTTCCGGAGCTGAGAAGCGCCCCATTTCCTCCGTCCTCATCGTCATCGCTATGCAGACTGAGGCGCTTCCGGTGGTGAATAAGTTTAACCTGACTGAGGTCCCTGACTCCGGACTCAATGCAAGCTCAAGAACTCAAGGTCAGCTAATGTAA

Protein Analysis

65

Amino Acids

6.83

Weight (kDa)

4.75

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018684)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003008.1_g000014 Rmu_sc0018528.1_g000002
rosa_samantha Rh1CG025000 Rh1DG046600 Rh5BG425400 Rh5BG533100 Rh5CG449500 Rh5CG556700
rosa_wichuraiana Rw1G001550 Rw7G037150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 32
AccIII TCCGGA 2 cut(s) 50, 155
AciI CCGC 2 cut(s) 30, 32
AcyI GRCGYC 2 cut(s) 27, 39
AfiI CCNNNNNNNGG 2 cut(s) 142, 155
AluBI AGCT 3 cut(s) 56, 171, 190
AluI AGCT 3 cut(s) 56, 171, 190
Aor13HI TCCGGA 2 cut(s) 50, 155
AspLEI GCGC 2 cut(s) 66, 112
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 1 cut(s) 133
AvaII GGWCC 1 cut(s) 145
BceAI ACGGC 1 cut(s) 28
BfoI RGCGCY 2 cut(s) 67, 113
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 2 cut(s) 6, 147
BoxI GACNNNNGTC 1 cut(s) 143
BpuEI CTTGAG 2 cut(s) 157, 165
BsaHI GRCGYC 2 cut(s) 27, 39
BsaJI CCNNGG 2 cut(s) 30, 42
BsaWI WCCGGW 3 cut(s) 50, 115, 155
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 155
BseAI TCCGGA 2 cut(s) 50, 155
BseDI CCNNGG 2 cut(s) 30, 42
BseLI CCNNNNNNNGG 2 cut(s) 142, 155
BseMII CTCAG 3 cut(s) 48, 96, 132
Bsh1236I CGCG 1 cut(s) 32
BsiSI CCGG 3 cut(s) 51, 116, 156
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 142, 155
BsmFI GGGAC 1 cut(s) 131
Bsp13I TCCGGA 2 cut(s) 50, 155
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 2 cut(s) 30, 32
BspCNI CTCAG 3 cut(s) 49, 97, 133
BspEI TCCGGA 2 cut(s) 50, 155
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 2 cut(s) 6, 147
BssECI CCNNGG 2 cut(s) 30, 42
BssNI GRCGYC 2 cut(s) 27, 39
BssT1I CCWWGG 1 cut(s) 42
BstACI GRCGYC 2 cut(s) 27, 39
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 3 cut(s) 57, 105, 141
BstDSI CCRYGG 2 cut(s) 30, 42
BstENI CCTNNNNNAGG 1 cut(s) 140
BstFNI CGCG 1 cut(s) 32
BstH2I RGCGCY 2 cut(s) 67, 113
BstHHI GCGC 2 cut(s) 66, 112
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstPAI GACNNNNGTC 1 cut(s) 143
BstUI CGCG 1 cut(s) 32
BtgI CCRYGG 2 cut(s) 30, 42
BtgZI GCGATG 1 cut(s) 76
Cac8I GCNNGC 1 cut(s) 169
CfoI GCGC 2 cut(s) 66, 112
Cfr13I GGNCC 1 cut(s) 145
Cfr42I CCGCGG 1 cut(s) 33
CseI GACGC 2 cut(s) 35, 47
CviAII CATG 1 cut(s) 43
CviJI RGCY 4 cut(s) 5, 56, 171, 190
CviKI_1 RGCY 4 cut(s) 5, 56, 171, 190
DdeI CTNAG 3 cut(s) 57, 105, 141
Eco130I CCWWGG 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 46
FaiI YATR 2 cut(s) 44, 98
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 42
Fnu4HI GCNGC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 30
GlaI GCGC 2 cut(s) 65, 111
GluI GCNGC 1 cut(s) 30
HaeII RGCGCY 2 cut(s) 67, 113
HapII CCGG 3 cut(s) 51, 116, 156
HgaI GACGC 2 cut(s) 35, 47
HhaI GCGC 2 cut(s) 66, 112
Hin1I GRCGYC 2 cut(s) 27, 39
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 2 cut(s) 64, 110
HinP1I GCGC 2 cut(s) 64, 110
HinfI GANTC 2 cut(s) 152, 159
HpaII CCGG 3 cut(s) 51, 116, 156
HphI GGTGA 1 cut(s) 133
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 3 cut(s) 51, 156, 174
HpyCH4V TGCA 2 cut(s) 100, 167
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 3 cut(s) 57, 105, 141
Hsp92I GRCGYC 2 cut(s) 27, 39
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 2 cut(s) 64, 110
Kpn2I TCCGGA 2 cut(s) 50, 155
KspI CCGCGG 1 cut(s) 33
LmnI GCTCC 2 cut(s) 10, 53
LpnPI CCDG 5 cut(s) 64, 129, 149, 162, 169
MlyI GAGTC 2 cut(s) 146, 153
MnlI CCTC 6 cut(s) 18, 28, 85, 92, 100, 136
MroI TCCGGA 2 cut(s) 50, 155
MseI TTAA 1 cut(s) 132
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 3 cut(s) 51, 116, 156
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 1 cut(s) 38
NcoI CCATGG 1 cut(s) 42
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 2 cut(s) 6, 147
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 2 cut(s) 146, 153
PpsI GAGTC 2 cut(s) 146, 153
PpuMI RGGWCCY 1 cut(s) 145
PshAI GACNNNNGTC 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 145
PspN4I GGNNCC 2 cut(s) 6, 147
PspPI GGNCC 1 cut(s) 145
PspPPI RGGWCCY 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 95
SacII CCGCGG 1 cut(s) 33
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 1 cut(s) 30
Sau96I GGNCC 1 cut(s) 145
SchI GAGTC 2 cut(s) 146, 153
SetI ASST 6 cut(s) 58, 138, 147, 173, 187, 192
Sfr303I CCGCGG 1 cut(s) 33
SgrBI CCGCGG 1 cut(s) 33
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 2 cut(s) 172, 180
SmoI CTYRAG 2 cut(s) 172, 180
SsiI CCGC 2 cut(s) 30, 32
StyI CCWWGG 1 cut(s) 42
TauI GCSGC 1 cut(s) 32
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TspGWI ACGGA 1 cut(s) 67
VpaK11BI GGWCC 1 cut(s) 145
XagI CCTNNNNNAGG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.