Rh5DG284300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005363
Physical Location & Seq
Forward (+)
35075646 .. 35102904
27259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG284300.1

Sequence Viewer

Length: 1848 bp
ATGGAAACTAAGAAGAAAAAAGATGCAATGTCTAGGTTATGTAGTTCACTTGGCTTTAATAATAGTAGGGTGGTGGAGAGGACAAGGGAGAGAGGGGGAGGTTTAGCTGTGTTTTGGAAGGATAGTTTAGAAGTTGATGTGATGGATTCCACTTCGGGTTTCATTGATTACATGGTGACAACTAATGGTAAGAGGCTTCGAGTAACGGGGTTTTATGGAAACCCGACGACTGGTGAAAGGCACCATTCCTGGGAGGTTCTCTGTCGTTTATCTCATTTATTACCTATGGATGATTGGATCGTATTTGGTGATTTTAATGAGATCTTGAGGGATGAGGAGAAGAGTGCAGGAAGACTCCGACCCATGCATCAAATGAAACGGTTTGGTCAGGTGGTGAATGAGTGTTGTCTCAAAGAAATTGAATTTACAGGGCCTCTGTTCACCTGGAGTAATGGAACGGTTTCAGAAAGGTTGGATAGGGGTTTTCTAAATGTGGCTGCTGCTCTTTCTTATCCTAATGCACATGTGTTTCATCTAGATGTGAGGGCAAGCAACCATCTTCCTTTACTTTTTTATATGCTCGGCGTCGGGTTACGGCAAACTCCGTTAAGTAAGAGGAGATTTTTATTTGAAAGTTTTTGGGTTAAGGAAGATCAGTGTGGTGACATTGTTAAGAAGGATTGGAATAAAAAGGTGGTGGGGCATGTTCCAAGGAAAATTGAAGGCTTGAAAAGAGATCTACAGCATTTTCCTATGGATGTAGTTACAGAGGTGGATATGAGAAAGAGAGCTGCAATTAAAGATGAATTAGAGAAGTATGCGGCATATGAGGAATCCATTTGGAAGGAAAGGTCACGAGTTCAGTGGCTACAAGATGGTGATAAGAATACGAGGTATTTTCATGTTGTGGGTCGTGGGCAACAAAATAAAGTTCAGGGGGTCTTTGACGAATATGTAGAAGATCGAGCAATATACTTGGTTTTGATGGAAGAAGACTCGGTTGCAATCAATGGAGGCTCTCCATCTTCACGCCTCAACCTAAATAGTGAAGTTTCGAAGGCTCATGTAAAGCAATTGGAGGATTCTCATGGTCAACTTCTACAGAGCAATCTTGAGACCGGAAGCTACAGCAAGCTTTACAGCTTTAAACACGTTGCGAACGGGTTTGCTGTCCATACAACCCCTTCTCAGGTTGAAAAGCTCAAATATGCTCCAGGAGTAAAGCTGGTTGACAGAGATAGAGGAGCAAAGTTGATGACAACTCATGTGGCATCAGTTGCTGCTGGAAATGTTGGAGTTCCTGTGGTTGTGAATGGCTTCAGTTATGGACGAGCTAGTGGAATGGCACCACGTGCGCGGTTCTCAACCACTTCAGAAATTTCATACAACTCTACCCAGGAGCTCGATCTTTTGTCCTTCATTAAATCGTCATTGGATGAGCGTGAAGGCCCATGCCATTGTTGGTTGAATAGGTCCAAAGGCAACAGACATTTCCTTGGAAGAGATGGAACTTTCTTGGTTCTTTCTGGTCGGTGTGAAACTTTCTTAACAACTTGTGAATCTGTTGTGATGTTAGAAAAAGTGAAGTCACTTCAACAGAGGTATCCTCAGCTCCATGTCATGGGTTTTCTGTCAGGCAATTTAGTTAGTTTAGCAGCTGCTCGAAGCCACCTAATCCAATATGTAGTAAAGGAATATGTCACTTTTCCTGTCATTTTGTCTAACAGGACCTTCCCTGAGATGGACAATGGGGCCTGCTACATTTTATTCAAAGATTTTGAAAGTCCAGTGTTTTTCCATGAGAAGGACCTTGATCTCAGAATTCTTAACAAAGGTTCAATAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

615

Amino Acids

69.93

Weight (kDa)

8.7

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 351 - 418 3.5e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 240, 1345
AccB7I CCANNNNNTGG 1 cut(s) 1621
AccII CGCG 1 cut(s) 1357
AciI CCGC 2 cut(s) 821, 1357
AclWI GGATC 1 cut(s) 305
AcsI RAATTY 3 cut(s) 422, 1377, 1821
AcuI CTGAAG 2 cut(s) 1303, 1356
AcvI CACGTG 1 cut(s) 1352
AcyI GRCGYC 1 cut(s) 585
AdeI CACNNNGTG 1 cut(s) 1352
AfiI CCNNNNNNNGG 6 cut(s) 230, 250, 1189, 1621, 1741, 1804
AflIII ACRYGT 2 cut(s) 523, 1150
AjnI CCWGG 4 cut(s) 248, 443, 1213, 1395
Alw21I GWGCWC 1 cut(s) 1404
Alw26I GTCTC 2 cut(s) 413, 1109
AlwI GGATC 1 cut(s) 305
AlwNI CAGNNNCTG 1 cut(s) 1280
AoxI GGCC 3 cut(s) 431, 1447, 1752
ApeKI GCWGC 6 cut(s) 497, 500, 791, 1280, 1655, 1658
ApoI RAATTY 3 cut(s) 422, 1377, 1821
Asp700I GAANNNNTTC 2 cut(s) 460, 1316
AspLEI GCGC 1 cut(s) 1357
AspS9I GGNCC 6 cut(s) 431, 1448, 1473, 1728, 1752, 1807
AsuHPI GGTGA 7 cut(s) 187, 245, 320, 406, 433, 674, 890
AsuII TTCGAA 1 cut(s) 1055
AvaII GGWCC 3 cut(s) 1473, 1728, 1807
BaeI ACNNNNGTAYC 2 cut(s) 1586, 1619
BanI GGYRCC 2 cut(s) 240, 1345
BanII GRGCYC 1 cut(s) 1404
BauI CACGAG 1 cut(s) 855
BbrPI CACGTG 1 cut(s) 1352
BbsI GAAGAC 2 cut(s) 358, 999
Bbv12I GWGCWC 1 cut(s) 1404
BbvCI CCTCAGC 1 cut(s) 1608
BbvI GCAGC 6 cut(s) 484, 487, 778, 1267, 1645, 1667
BccI CCATC 7 cut(s) 136, 564, 869, 979, 1030, 1499, 1735
BceAI ACGGC 1 cut(s) 611
BciT130I CCWGG 4 cut(s) 250, 445, 1215, 1397
BciVI GTATCC 1 cut(s) 1614
BcoDI GTCTC 2 cut(s) 413, 1109
BfaI CTAG 3 cut(s) 33, 536, 1335
BfmI CTRYAG 3 cut(s) 740, 1100, 1126
BfuI GTATCC 1 cut(s) 1614
BglII AGATCT 2 cut(s) 321, 736
BisI GCNGC 7 cut(s) 498, 501, 792, 822, 1281, 1656, 1659
BlsI GCNGC 7 cut(s) 499, 502, 793, 823, 1282, 1657, 1660
Bme1390I CCNGG 4 cut(s) 250, 445, 1215, 1397
Bme18I GGWCC 3 cut(s) 1473, 1728, 1807
BmgT120I GGNCC 6 cut(s) 431, 1448, 1473, 1728, 1752, 1807
BmiI GGNNCC 3 cut(s) 242, 1347, 1753
BmrFI CCNGG 4 cut(s) 250, 445, 1215, 1397
BmsI GCATC 3 cut(s) 13, 376, 1280
BpiI GAAGAC 2 cut(s) 358, 999
BplI GAGNNNNNCTC 2 cut(s) 1591, 1623
BpmI CTGGAG 2 cut(s) 466, 1197
Bpu10I CCTNAGC 1 cut(s) 1608
Bpu14I TTCGAA 1 cut(s) 1055
BpuEI CTTGAG 2 cut(s) 346, 1133
BsaAI YACGTR 1 cut(s) 1352
BsaHI GRCGYC 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 1109
BsaJI CCNNGG 4 cut(s) 249, 710, 1395, 1495
BsaWI WCCGGW 1 cut(s) 1118
BsaXI ACNNNNNCTCC 2 cut(s) 1287, 1317
Bsc4I CCNNNNNNNGG 6 cut(s) 230, 250, 1189, 1621, 1741, 1804
Bse1I ACTGG 2 cut(s) 235, 1787
Bse3DI GCAATG 1 cut(s) 33
BseBI CCWGG 4 cut(s) 250, 445, 1215, 1397
BseDI CCNNGG 4 cut(s) 249, 710, 1395, 1495
BseGI GGATG 4 cut(s) 295, 337, 763, 1441
BseLI CCNNNNNNNGG 6 cut(s) 230, 250, 1189, 1621, 1741, 1804
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 4 cut(s) 1202, 1622, 1728, 1831
BseNI ACTGG 2 cut(s) 235, 1787
BseRI GAGGAG 3 cut(s) 350, 631, 1257
BseXI GCAGC 6 cut(s) 484, 487, 778, 1267, 1645, 1667
BsgI GTGCAG 1 cut(s) 366
Bsh1236I CGCG 1 cut(s) 1357
BshFI GGCC 3 cut(s) 433, 1449, 1754
BshNI GGYRCC 2 cut(s) 240, 1345
BsiHKAI GWGCWC 1 cut(s) 1404
BsiSI CCGG 1 cut(s) 1119
BslI CCNNNNNNNGG 6 cut(s) 230, 250, 1189, 1621, 1741, 1804
BsmAI GTCTC 2 cut(s) 413, 1109
BsnI GGCC 3 cut(s) 433, 1449, 1754
Bso31I GGTCTC 1 cut(s) 1109
Bsp119I TTCGAA 1 cut(s) 1055
Bsp1286I GDGCHC 1 cut(s) 1404
Bsp143I GATC 7 cut(s) 297, 321, 652, 736, 961, 1405, 1813
BspACI CCGC 2 cut(s) 821, 1357
BspANI GGCC 3 cut(s) 433, 1449, 1754
BspCNI CTCAG 4 cut(s) 1201, 1621, 1729, 1830
BspFNI CGCG 1 cut(s) 1357
BspLI GGNNCC 3 cut(s) 242, 1347, 1753
BspPI GGATC 1 cut(s) 305
BspT104I TTCGAA 1 cut(s) 1055
BspT107I GGYRCC 2 cut(s) 240, 1345
BspTNI GGTCTC 1 cut(s) 1109
BsrDI GCAATG 1 cut(s) 33
BsrI ACTGG 2 cut(s) 235, 1787
BssECI CCNNGG 4 cut(s) 249, 710, 1395, 1495
BssMI GATC 7 cut(s) 297, 321, 652, 736, 961, 1405, 1813
BssNI GRCGYC 1 cut(s) 585
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 2 cut(s) 710, 1495
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 4 cut(s) 250, 445, 1215, 1397
Bst4CI ACNGT 2 cut(s) 381, 460
Bst6I CTCTTC 2 cut(s) 335, 1495
BstACI GRCGYC 1 cut(s) 585
BstAPI GCANNNNNTGC 2 cut(s) 1277, 1352
BstBAI YACGTR 1 cut(s) 1352
BstBI TTCGAA 1 cut(s) 1055
BstC8I GCNNGC 3 cut(s) 550, 1133, 1756
BstDEI CTNAG 5 cut(s) 9, 1188, 1608, 1737, 1817
BstF5I GGATG 4 cut(s) 295, 337, 763, 1441
BstFNI CGCG 1 cut(s) 1357
BstHHI GCGC 1 cut(s) 1357
BstKTI GATC 7 cut(s) 300, 324, 655, 739, 964, 1408, 1816
BstMAI GTCTC 2 cut(s) 413, 1109
BstMBI GATC 7 cut(s) 297, 321, 652, 736, 961, 1405, 1813
BstMWI GCNNNNNNNGC 2 cut(s) 1277, 1352
BstNI CCWGG 4 cut(s) 250, 445, 1215, 1397
BstNSI RCATGY 2 cut(s) 527, 707
BstSCI CCNGG 4 cut(s) 248, 443, 1213, 1395
BstSFI CTRYAG 3 cut(s) 740, 1100, 1126
BstUI CGCG 1 cut(s) 1357
BstV1I GCAGC 6 cut(s) 484, 487, 778, 1267, 1645, 1667
BstV2I GAAGAC 2 cut(s) 358, 999
BstX2I RGATCY 2 cut(s) 321, 736
BstYI RGATCY 2 cut(s) 321, 736
BsuI GTATCC 1 cut(s) 1614
BsuRI GGCC 3 cut(s) 433, 1449, 1754
BtsCI GGATG 4 cut(s) 295, 337, 763, 1441
BtsIMutI CAGTG 3 cut(s) 662, 869, 1794
Cac8I GCNNGC 3 cut(s) 550, 1133, 1756
CaiI CAGNNNCTG 1 cut(s) 1280
CfoI GCGC 1 cut(s) 1357
Cfr13I GGNCC 6 cut(s) 431, 1448, 1473, 1728, 1752, 1807
CseI GACGC 1 cut(s) 574
CspCI CAANNNNNGTGG 2 cut(s) 1248, 1283
DdeI CTNAG 5 cut(s) 9, 1188, 1608, 1737, 1817
DpnI GATC 7 cut(s) 299, 323, 654, 738, 963, 1407, 1815
DpnII GATC 7 cut(s) 297, 321, 652, 736, 961, 1405, 1813
DraI TTTAAA 1 cut(s) 1147
DraIII CACNNNGTG 1 cut(s) 1352
Eam1104I CTCTTC 2 cut(s) 335, 1495
EarI CTCTTC 2 cut(s) 335, 1495
Ecl136II GAGCTC 1 cut(s) 1402
Eco130I CCWWGG 2 cut(s) 710, 1495
Eco24I GRGCYC 1 cut(s) 1404
Eco31I GGTCTC 1 cut(s) 1109
Eco47I GGWCC 3 cut(s) 1473, 1728, 1807
Eco53kI GAGCTC 1 cut(s) 1402
Eco57I CTGAAG 2 cut(s) 1303, 1356
Eco72I CACGTG 1 cut(s) 1352
EcoICRI GAGCTC 1 cut(s) 1402
EcoO109I RGGNCCY 4 cut(s) 431, 1728, 1752, 1807
EcoRI GAATTC 1 cut(s) 1821
EcoRII CCWGG 4 cut(s) 248, 443, 1213, 1395
EcoT14I CCWWGG 2 cut(s) 710, 1495
EcoT22I ATGCAT 1 cut(s) 369
EcoT38I GRGCYC 1 cut(s) 1404
ErhI CCWWGG 2 cut(s) 710, 1495
FauNDI CATATG 1 cut(s) 826
Fnu4HI GCNGC 7 cut(s) 498, 501, 792, 822, 1281, 1656, 1659
FokI GGATG 4 cut(s) 302, 344, 770, 1448
FriOI GRGCYC 1 cut(s) 1404
Fsp4HI GCNGC 7 cut(s) 498, 501, 792, 822, 1281, 1656, 1659
FspBI CTAG 3 cut(s) 33, 536, 1335
GlaI GCGC 1 cut(s) 1356
GluI GCNGC 7 cut(s) 498, 501, 792, 822, 1281, 1656, 1659
GsuI CTGGAG 2 cut(s) 466, 1197
HaeIII GGCC 3 cut(s) 433, 1449, 1754
HapII CCGG 1 cut(s) 1119
HgaI GACGC 1 cut(s) 574
HhaI GCGC 1 cut(s) 1357
Hin1I GRCGYC 1 cut(s) 585
Hin6I GCGC 1 cut(s) 1355
HinP1I GCGC 1 cut(s) 1355
HincII GTYRAC 2 cut(s) 1094, 1231
HindII GTYRAC 2 cut(s) 1094, 1231
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 6 cut(s) 146, 354, 833, 995, 1082, 1559
HpaII CCGG 1 cut(s) 1119
HphI GGTGA 7 cut(s) 187, 245, 320, 406, 433, 674, 890
Hpy166II GTNNAC 4 cut(s) 47, 441, 1094, 1231
Hpy188I TCNGA 4 cut(s) 359, 466, 1375, 1820
Hpy188III TCNNGA 4 cut(s) 325, 536, 855, 1112
Hpy8I GTNNAC 4 cut(s) 47, 441, 1094, 1231
Hpy99I CGWCG 2 cut(s) 229, 590
HpyCH4III ACNGT 2 cut(s) 381, 460
HpyCH4IV ACGT 2 cut(s) 1152, 1351
HpyCH4V TGCA 6 cut(s) 26, 347, 367, 521, 794, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 1277, 1352
HpyF3I CTNAG 5 cut(s) 9, 1188, 1608, 1737, 1817
HpySE526I ACGT 2 cut(s) 1152, 1351
Hsp92I GRCGYC 1 cut(s) 585
HspAI GCGC 1 cut(s) 1355
Kzo9I GATC 7 cut(s) 297, 321, 652, 736, 961, 1405, 1813
LmnI GCTCC 4 cut(s) 1216, 1244, 1399, 1617
Lsp1109I GCAGC 6 cut(s) 484, 487, 778, 1267, 1645, 1667
LweI GCATC 3 cut(s) 13, 376, 1280
MaeI CTAG 3 cut(s) 33, 536, 1335
MaeII ACGT 2 cut(s) 1152, 1351
MaeIII GTNAC 8 cut(s) 175, 202, 591, 662, 763, 852, 1587, 1699
MalI GATC 7 cut(s) 299, 323, 654, 738, 963, 1407, 1815
MboI GATC 7 cut(s) 297, 321, 652, 736, 961, 1405, 1813
MfeI CAATTG 1 cut(s) 1073
MflI RGATCY 2 cut(s) 321, 736
MhlI GDGCHC 1 cut(s) 1404
MluCI AATT 9 cut(s) 417, 422, 717, 795, 806, 1073, 1377, 1639, 1821
MlyI GAGTC 2 cut(s) 348, 989
MmeI TCCRAC 3 cut(s) 382, 453, 1273
Mph1103I ATGCAT 1 cut(s) 369
MroXI GAANNNNTTC 2 cut(s) 460, 1316
MslI CAYNNNNRTG 1 cut(s) 537
MspA1I CMGCKG 1 cut(s) 1658
MspI CCGG 1 cut(s) 1119
MspR9I CCNGG 4 cut(s) 250, 445, 1215, 1397
MunI CAATTG 1 cut(s) 1073
MvaI CCWGG 4 cut(s) 250, 445, 1215, 1397
MvnI CGCG 1 cut(s) 1357
MwoI GCNNNNNNNGC 2 cut(s) 1277, 1352
NdeI CATATG 1 cut(s) 826
NdeII GATC 7 cut(s) 297, 321, 652, 736, 961, 1405, 1813
NlaIV GGNNCC 3 cut(s) 242, 1347, 1753
NmeAIII GCCGAG 1 cut(s) 561
NmuCI GTSAC 5 cut(s) 175, 662, 852, 1587, 1699
NsiI ATGCAT 1 cut(s) 369
NspI RCATGY 2 cut(s) 527, 707
NspV TTCGAA 1 cut(s) 1055
PciI ACATGT 1 cut(s) 523
PdmI GAANNNNTTC 2 cut(s) 460, 1316
PfeI GAWTC 4 cut(s) 146, 833, 1082, 1559
PflMI CCANNNNNTGG 1 cut(s) 1621
PfoI TCCNGGA 1 cut(s) 1213
PkrI GCNGC 7 cut(s) 499, 502, 793, 823, 1282, 1657, 1660
PleI GAGTC 2 cut(s) 348, 989
PmaCI CACGTG 1 cut(s) 1352
PmlI CACGTG 1 cut(s) 1352
PpsI GAGTC 2 cut(s) 348, 989
Ppu21I YACGTR 1 cut(s) 1352
PpuMI RGGWCCY 2 cut(s) 1728, 1807
PscI ACATGT 1 cut(s) 523
Psp124BI GAGCTC 1 cut(s) 1404
Psp5II RGGWCCY 2 cut(s) 1728, 1807
Psp6I CCWGG 4 cut(s) 248, 443, 1213, 1395
PspCI CACGTG 1 cut(s) 1352
PspGI CCWGG 4 cut(s) 248, 443, 1213, 1395
PspN4I GGNNCC 3 cut(s) 242, 1347, 1753
PspPI GGNCC 6 cut(s) 431, 1448, 1473, 1728, 1752, 1807
PspPPI RGGWCCY 2 cut(s) 1728, 1807
PstNI CAGNNNCTG 1 cut(s) 1280
PsuI RGATCY 2 cut(s) 321, 736
PvuII CAGCTG 1 cut(s) 1658
RseI CAYNNNNRTG 1 cut(s) 537
SacI GAGCTC 1 cut(s) 1404
SatI GCNGC 7 cut(s) 498, 501, 792, 822, 1281, 1656, 1659
Sau3AI GATC 7 cut(s) 297, 321, 652, 736, 961, 1405, 1813
Sau96I GGNCC 6 cut(s) 431, 1448, 1473, 1728, 1752, 1807
SchI GAGTC 2 cut(s) 348, 989
ScrFI CCNGG 4 cut(s) 250, 445, 1215, 1397
SduI GDGCHC 1 cut(s) 1404
SfaNI GCATC 3 cut(s) 13, 376, 1280
SfcI CTRYAG 3 cut(s) 740, 1100, 1126
SfuI TTCGAA 1 cut(s) 1055
SinI GGWCC 3 cut(s) 1473, 1728, 1807
SmiMI CAYNNNNRTG 1 cut(s) 537
SmlI CTYRAG 2 cut(s) 325, 1112
SmoI CTYRAG 2 cut(s) 325, 1112
Sse9I AATT 9 cut(s) 417, 422, 717, 795, 806, 1073, 1377, 1639, 1821
SsiI CCGC 2 cut(s) 821, 1357
SspMI CTAG 3 cut(s) 33, 536, 1335
SstI GAGCTC 1 cut(s) 1404
StyD4I CCNGG 4 cut(s) 248, 443, 1213, 1395
StyI CCWWGG 2 cut(s) 710, 1495
TaaI ACNGT 2 cut(s) 381, 460
TaiI ACGT 2 cut(s) 1155, 1354
TaqI TCGA 5 cut(s) 199, 964, 1055, 1404, 1663
TasI AATT 9 cut(s) 417, 422, 717, 795, 806, 1073, 1377, 1639, 1821
TauI GCSGC 1 cut(s) 824
TfiI GAWTC 4 cut(s) 146, 833, 1082, 1559
TscAI CASTG 3 cut(s) 662, 869, 1794
TseFI GTSAC 5 cut(s) 175, 662, 852, 1587, 1699
TseI GCWGC 6 cut(s) 497, 500, 791, 1280, 1655, 1658
Tsp45I GTSAC 5 cut(s) 175, 662, 852, 1587, 1699
TspDTI ATGAA 7 cut(s) 151, 389, 521, 819, 890, 1371, 1408
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 3 cut(s) 662, 869, 1794
Van91I CCANNNNNTGG 1 cut(s) 1621
VpaK11BI GGWCC 3 cut(s) 1473, 1728, 1807
XapI RAATTY 3 cut(s) 422, 1377, 1821
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 2 cut(s) 527, 707
XcmI CCANNNNNNNNNTGG 1 cut(s) 1458
XmnI GAANNNNTTC 2 cut(s) 460, 1316
XspI CTAG 3 cut(s) 33, 536, 1335
Zsp2I ATGCAT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.