Rorug06G0169500

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003845
Physical Location & Seq
Forward (+)
25050649 .. 25055193
4545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0169500.1

Sequence Viewer

Length: 1089 bp
ATGGCTGCGACCAAGACTAGGACGAGTAGCTTCTCTCGCAGTTTTTCGGGTGCAAGCTCACCTCAAATCCCAGGACTCAAGCATGGACCCAATGGCACAATGTTTGTTTCATCTGGGATTCCAGACCTTGACAAAATTTTAGGTGGTGGTTTTGCTCTAGGAAGCCTAGTAATGGTGATGGAAGATGCAGAAGCACCTCATCATATGCTTTTACTTAGGAATTTCATGTCTCAAGGACTCGTTCACAACCAACCCCTTCTCTATGCAAGCCCAGCCAAGGACCCAAAACCGTTTCTTGGTACTTTGCCTAGTCCAGCGGTACCCAAAGATGAAAAGTCTAGTCATCGAGACCCTGATCAGGAGAAAGGGTTGAGGATAGCTTGGCAATACAAGAAGTATTTTGGTGAAAATCAGCAGGGTTTTGATAGTCAAAATGGGAAACATGAGTTCAGCAACAACTTCGACTTGCGGAAGCCCTTGGAGAGGCAGTTTCTTACGGGCAAGCGAATAGATTGTGCTAGCATTCTTGATTCTCCAAATCTTGTCGCACTTTATGATCGTTGTGCTACATTTTTATCACAATTTCCAAGAAGTGACAGCAACGTTTCTTGTGTTGGTCGTATTGCCATTCAATCATTCTGTGCTCCACAGTGTGGATATTCCAGCCTGGAATGGGACATGCTTTCCTTCCTTAGATCTCTAAAAAGCATGCTACGATCTTCAAATGCAGTTGCTGCTGTGACATTTCCGCCTAGTCTTCTTTCATCATCCTCCTCTACAAGATGGCAGCACATGGCAGACACCTTGCTGTCAGTTAAAGCAATTCCAGATGAGGACAAGGAATTGGCAACGCTCCTTACTGGTTACCAGGACATGGTTGGCCTTCTTAATGTGCAGAAAGTAGCGCAAATTAACACACAGGTTCCTGTCATTCTTGAGGCAACAACTTTCTCAATAAAGCTGCAAAAGCGGAGGTTTTTGGTTTTAGAATGTCTAAACCAGGCCCCTATCGATGGTTCTAGTGGGAGTTCATATGGCACTTCTGGTAGTTGTTCTGGGTCCTCTAAAACTGGATATCTTGATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

362

Amino Acids

39.52

Weight (kDa)

8.59

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAXNEB PF05625 21 - 362 9.2e-93 PAXNEB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 319
AccB1I GGYRCC 1 cut(s) 319
AccB7I CCANNNNNTGG 2 cut(s) 653, 874
AciI CCGC 4 cut(s) 317, 469, 749, 970
AclI AACGTT 1 cut(s) 603
AcsI RAATTY 2 cut(s) 135, 220
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 2 cut(s) 301, 321
AgsI TTSAA 2 cut(s) 632, 723
AjnI CCWGG 4 cut(s) 70, 666, 867, 999
AluBI AGCT 4 cut(s) 30, 57, 380, 961
AluI AGCT 4 cut(s) 30, 57, 380, 961
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 2 cut(s) 234, 342
AlwNI CAGNNNCTG 1 cut(s) 734
AoxI GGCC 2 cut(s) 880, 1002
ApeKI GCWGC 4 cut(s) 5, 734, 787, 961
ApoI RAATTY 2 cut(s) 135, 220
Asp718I GGTACC 1 cut(s) 319
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 4 cut(s) 86, 280, 1003, 1059
AsuHPI GGTGA 3 cut(s) 51, 187, 416
AsuNHI GCTAGC 1 cut(s) 518
AvaII GGWCC 3 cut(s) 86, 280, 1059
BanI GGYRCC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 3 cut(s) 721, 799, 948
BccI CCATC 3 cut(s) 172, 777, 1007
BcgI CGANNNNNNTGC 2 cut(s) 442, 476
BciT130I CCWGG 4 cut(s) 72, 668, 869, 1001
BclI TGATCA 1 cut(s) 355
BcoDI GTCTC 2 cut(s) 234, 342
BfaI CTAG 8 cut(s) 18, 158, 167, 309, 339, 519, 753, 1020
BglII AGATCT 1 cut(s) 695
BisI GCNGC 4 cut(s) 6, 735, 788, 962
BlsI GCNGC 4 cut(s) 7, 736, 789, 963
Bme1390I CCNGG 4 cut(s) 72, 668, 869, 1001
Bme18I GGWCC 3 cut(s) 86, 280, 1059
BmgT120I GGNCC 4 cut(s) 86, 280, 1003, 1059
BmiI GGNNCC 6 cut(s) 88, 282, 321, 924, 1005, 1060
BmrFI CCNGG 4 cut(s) 72, 668, 869, 1001
BmsI GCATC 1 cut(s) 175
BmtI GCTAGC 1 cut(s) 522
BpiI GAAGAC 1 cut(s) 749
BpuEI CTTGAG 3 cut(s) 62, 216, 956
Bsa29I ATCGAT 1 cut(s) 1011
BsaI GGTCTC 1 cut(s) 342
BsaJI CCNNGG 3 cut(s) 70, 276, 477
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 473, 503
Bse1I ACTGG 2 cut(s) 865, 1075
BseBI CCWGG 4 cut(s) 72, 668, 869, 1001
BseCI ATCGAT 1 cut(s) 1011
BseDI CCNNGG 3 cut(s) 70, 276, 477
BseGI GGATG 1 cut(s) 767
BseNI ACTGG 2 cut(s) 865, 1075
BseRI GAGGAG 1 cut(s) 763
BseXI GCAGC 3 cut(s) 721, 799, 948
BseYI CCCAGC 1 cut(s) 271
BsgI GTGCAG 1 cut(s) 914
BshFI GGCC 2 cut(s) 882, 1004
BshNI GGYRCC 1 cut(s) 319
BshVI ATCGAT 1 cut(s) 1011
BsiHKAI GWGCWC 1 cut(s) 646
BslFI GGGAC 1 cut(s) 689
BsmAI GTCTC 2 cut(s) 234, 342
BsmFI GGGAC 1 cut(s) 689
BsmI GAATGC 1 cut(s) 522
BsnI GGCC 2 cut(s) 882, 1004
Bso31I GGTCTC 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 4 cut(s) 355, 556, 695, 716
BspACI CCGC 4 cut(s) 317, 469, 749, 970
BspANI GGCC 2 cut(s) 882, 1004
BspDI ATCGAT 1 cut(s) 1011
BspLI GGNNCC 6 cut(s) 88, 282, 321, 924, 1005, 1060
BspOI GCTAGC 1 cut(s) 522
BspT107I GGYRCC 1 cut(s) 319
BspTNI GGTCTC 1 cut(s) 342
BsrI ACTGG 2 cut(s) 865, 1075
BssECI CCNNGG 3 cut(s) 70, 276, 477
BssMI GATC 4 cut(s) 355, 556, 695, 716
BssT1I CCWWGG 2 cut(s) 276, 477
Bst2UI CCWGG 4 cut(s) 72, 668, 869, 1001
Bst4CI ACNGT 2 cut(s) 291, 651
BstAPI GCANNNNNTGC 1 cut(s) 734
BstC8I GCNNGC 5 cut(s) 55, 268, 503, 520, 710
BstDEI CTNAG 2 cut(s) 215, 692
BstEII GGTNACC 1 cut(s) 863
BstENI CCTNNNNNAGG 1 cut(s) 481
BstF5I GGATG 1 cut(s) 767
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 4 cut(s) 358, 559, 698, 719
BstMAI GTCTC 2 cut(s) 234, 342
BstMBI GATC 4 cut(s) 355, 556, 695, 716
BstMWI GCNNNNNNNGC 4 cut(s) 36, 272, 734, 967
BstNI CCWGG 4 cut(s) 72, 668, 869, 1001
BstNSI RCATGY 2 cut(s) 682, 712
BstPI GGTNACC 1 cut(s) 863
BstSCI CCNGG 4 cut(s) 70, 666, 867, 999
BstV1I GCAGC 3 cut(s) 721, 799, 948
BstV2I GAAGAC 1 cut(s) 749
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
Bsu15I ATCGAT 1 cut(s) 1011
BsuRI GGCC 2 cut(s) 882, 1004
BsuTUI ATCGAT 1 cut(s) 1011
BtsCI GGATG 1 cut(s) 767
BtsIMutI CAGTG 1 cut(s) 656
Cac8I GCNNGC 5 cut(s) 55, 268, 503, 520, 710
CaiI CAGNNNCTG 1 cut(s) 734
CfoI GCGC 1 cut(s) 907
Cfr13I GGNCC 4 cut(s) 86, 280, 1003, 1059
ClaI ATCGAT 1 cut(s) 1011
Csp6I GTAC 2 cut(s) 300, 320
CviAII CATG 7 cut(s) 83, 226, 443, 679, 709, 793, 874
CviQI GTAC 2 cut(s) 300, 320
DdeI CTNAG 2 cut(s) 215, 692
DpnI GATC 4 cut(s) 357, 558, 697, 718
DpnII GATC 4 cut(s) 355, 556, 695, 716
DraIII CACNNNGTG 1 cut(s) 653
EciI GGCGGA 1 cut(s) 738
Eco130I CCWWGG 2 cut(s) 276, 477
Eco31I GGTCTC 1 cut(s) 342
Eco32I GATATC 1 cut(s) 1076
Eco47I GGWCC 3 cut(s) 86, 280, 1059
Eco91I GGTNACC 1 cut(s) 863
EcoNI CCTNNNNNAGG 1 cut(s) 481
EcoO109I RGGNCCY 3 cut(s) 280, 1003, 1059
EcoO65I GGTNACC 1 cut(s) 863
EcoRII CCWGG 4 cut(s) 70, 666, 867, 999
EcoRV GATATC 1 cut(s) 1076
EcoT14I CCWWGG 2 cut(s) 276, 477
ErhI CCWWGG 2 cut(s) 276, 477
FaeI CATG 7 cut(s) 86, 229, 446, 682, 712, 796, 877
FaqI GGGAC 1 cut(s) 689
FatI CATG 7 cut(s) 82, 225, 442, 678, 708, 792, 873
FauNDI CATATG 2 cut(s) 204, 1033
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 4 cut(s) 6, 735, 788, 962
FokI GGATG 1 cut(s) 754
Fsp4HI GCNGC 4 cut(s) 6, 735, 788, 962
FspBI CTAG 8 cut(s) 18, 158, 167, 309, 339, 519, 753, 1020
GlaI GCGC 1 cut(s) 906
GluI GCNGC 4 cut(s) 6, 735, 788, 962
GsaI CCCAGC 1 cut(s) 275
HaeIII GGCC 2 cut(s) 882, 1004
HhaI GCGC 1 cut(s) 907
Hin1II CATG 7 cut(s) 86, 229, 446, 682, 712, 796, 877
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HinfI GANTC 4 cut(s) 75, 118, 237, 530
HphI GGTGA 3 cut(s) 51, 187, 416
Hpy166II GTNNAC 1 cut(s) 244
Hpy188III TCNNGA 7 cut(s) 122, 347, 359, 527, 827, 935, 1079
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 3 cut(s) 266, 697, 893
HpyCH4III ACNGT 2 cut(s) 291, 651
HpyCH4IV ACGT 1 cut(s) 603
HpyCH4V TGCA 6 cut(s) 53, 188, 266, 728, 895, 964
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 272, 734, 967
HpyF3I CTNAG 2 cut(s) 215, 692
HpySE526I ACGT 1 cut(s) 603
Hsp92II CATG 7 cut(s) 86, 229, 446, 682, 712, 796, 877
HspAI GCGC 1 cut(s) 905
KpnI GGTACC 1 cut(s) 323
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 4 cut(s) 355, 556, 695, 716
LmnI GCTCC 2 cut(s) 649, 858
Lsp1109I GCAGC 3 cut(s) 721, 799, 948
LweI GCATC 1 cut(s) 175
MaeI CTAG 8 cut(s) 18, 158, 167, 309, 339, 519, 753, 1020
MaeII ACGT 1 cut(s) 603
MaeIII GTNAC 3 cut(s) 593, 739, 863
MalI GATC 4 cut(s) 357, 558, 697, 718
MboI GATC 4 cut(s) 355, 556, 695, 716
MboII GAAGA 3 cut(s) 194, 711, 749
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 6 cut(s) 135, 220, 581, 822, 842, 909
MlyI GAGTC 2 cut(s) 69, 231
MseI TTAA 3 cut(s) 816, 888, 912
MspA1I CMGCKG 1 cut(s) 317
MspR9I CCNGG 4 cut(s) 72, 668, 869, 1001
Mva1269I GAATGC 1 cut(s) 522
MvaI CCWGG 4 cut(s) 72, 668, 869, 1001
MwoI GCNNNNNNNGC 4 cut(s) 36, 272, 734, 967
NdeI CATATG 2 cut(s) 204, 1033
NdeII GATC 4 cut(s) 355, 556, 695, 716
NheI GCTAGC 1 cut(s) 518
NlaIII CATG 7 cut(s) 86, 229, 446, 682, 712, 796, 877
NlaIV GGNNCC 6 cut(s) 88, 282, 321, 924, 1005, 1060
NmuCI GTSAC 2 cut(s) 593, 739
NspI RCATGY 2 cut(s) 682, 712
PaeI GCATGC 1 cut(s) 712
PctI GAATGC 1 cut(s) 522
PfeI GAWTC 2 cut(s) 118, 530
PflMI CCANNNNNTGG 2 cut(s) 653, 874
PkrI GCNGC 4 cut(s) 7, 736, 789, 963
PleI GAGTC 2 cut(s) 69, 231
PpsI GAGTC 2 cut(s) 69, 231
PpuMI RGGWCCY 2 cut(s) 280, 1059
Psp1406I AACGTT 1 cut(s) 603
Psp5II RGGWCCY 2 cut(s) 280, 1059
Psp6I CCWGG 4 cut(s) 70, 666, 867, 999
PspEI GGTNACC 1 cut(s) 863
PspFI CCCAGC 1 cut(s) 271
PspGI CCWGG 4 cut(s) 70, 666, 867, 999
PspN4I GGNNCC 6 cut(s) 88, 282, 321, 924, 1005, 1060
PspPI GGNCC 4 cut(s) 86, 280, 1003, 1059
PspPPI RGGWCCY 2 cut(s) 280, 1059
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 1 cut(s) 695
RsaI GTAC 2 cut(s) 301, 321
RsaNI GTAC 2 cut(s) 300, 320
SaqAI TTAA 3 cut(s) 816, 888, 912
SatI GCNGC 4 cut(s) 6, 735, 788, 962
Sau3AI GATC 4 cut(s) 355, 556, 695, 716
Sau96I GGNCC 4 cut(s) 86, 280, 1003, 1059
SchI GAGTC 2 cut(s) 69, 231
ScrFI CCNGG 4 cut(s) 72, 668, 869, 1001
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 1 cut(s) 175
SinI GGWCC 3 cut(s) 86, 280, 1059
SmlI CTYRAG 3 cut(s) 77, 231, 935
SmoI CTYRAG 3 cut(s) 77, 231, 935
SphI GCATGC 1 cut(s) 712
Sse9I AATT 6 cut(s) 135, 220, 581, 822, 842, 909
SsiI CCGC 4 cut(s) 317, 469, 749, 970
SspMI CTAG 8 cut(s) 18, 158, 167, 309, 339, 519, 753, 1020
StyD4I CCNGG 4 cut(s) 70, 666, 867, 999
StyI CCWWGG 2 cut(s) 276, 477
TaaI ACNGT 2 cut(s) 291, 651
TaiI ACGT 1 cut(s) 606
TaqI TCGA 3 cut(s) 346, 462, 1011
TasI AATT 6 cut(s) 135, 220, 581, 822, 842, 909
TfiI GAWTC 2 cut(s) 118, 530
Tru1I TTAA 3 cut(s) 816, 888, 912
Tru9I TTAA 3 cut(s) 816, 888, 912
TscAI CASTG 1 cut(s) 656
TseFI GTSAC 2 cut(s) 593, 739
TseI GCWGC 4 cut(s) 5, 734, 787, 961
Tsp45I GTSAC 2 cut(s) 593, 739
TspDTI ATGAA 5 cut(s) 99, 214, 345, 753, 1020
TspRI CASTG 1 cut(s) 656
Van91I CCANNNNNTGG 2 cut(s) 653, 874
VpaK11BI GGWCC 3 cut(s) 86, 280, 1059
XagI CCTNNNNNAGG 1 cut(s) 481
XapI RAATTY 2 cut(s) 135, 220
XceI RCATGY 2 cut(s) 682, 712
XcmI CCANNNNNNNNNTGG 1 cut(s) 875
XspI CTAG 8 cut(s) 18, 158, 167, 309, 339, 519, 753, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.