Rorug06G0169600

Protein of unknown function (DUF3685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003845
Physical Location & Seq
Reverse (-)
25055359 .. 25056958
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0169600.1

Sequence Viewer

Length: 921 bp
ATGGTCAAGAATGAATGGGGCTTCTCTACCTATCCTCTGGTTCCCGGGCATGAGATTGTCGGTGAAGTGACGGAAGTAGGGAGCAATGTACAAAAATTCAAAGTTGGAGACAAAGTCGGTGTTGGATGCATAGTGGGAGCTTGCCGATCTTGTGATAGTTGTACCGACCATCTTGAGAACTACTGCCCCAAACAAATACTCACGTACAGTGCCAAGTACTATGACGGAACCACCACCTATGGCGGTTACTCTGACATTATGGTTGCAGATGAACACTTCGTACTCCGTATCCCGGACAACCTACCCCTTGATTGTGCTGCTCCTCTCCTATGTGCCGGAATCACAACCTACAGCCCGTTGAGATATTTTGGACTTGACAAGCCCGGCATGCATGTGGGCGTGGTTGGCCTAGGTGGTTTAGGCCACGTCGCAGTGAAGTTTGCCAAGGCAATGGGAGTGAAGGTTACAGTGATCAGTACGTCCCCTAATAAGAAGGAGGAAGCAGTTAAACACCTAGGAGCTGATTCGTTTTTGGTTAGTCGTGACCATGATAAAATGCAGGCTGCCATTGGTACCATGGATGGGATCATTGACACGGTTTCTGCACAACATCCTCTCTTGCCTTTGATTGGTTTGTTGAAGTCTCATGGAAAGCTTGTTATGGTTGGTGCACCAGAAAAGCCTCTTGAGCTTCCGGTTTTTCCTTTACTCATGGGAAGGAAGATGGTAGCTGGTAGCGGCATTGGAGGTATGAAGGAGACACAAGAGATGATCAATTTTGCAGCCAAGCACAACATAACAGCAGACATTGAGGTTATCCCAATTGACTACTTGAACACTGCCATGGAGCGCCTTGCCAAAGCAGACGTCAGATACCGTTTTGTCATTGACATTGGAAACACACTGAAGGCTAGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3685

Protein Analysis

306

Amino Acids

32.91

Weight (kDa)

6.55

Isoelectric Point (pI)

27.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 3 - 99 2.6e-19 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 121 - 171 7.4e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
AlaDh_PNT_C PF01262 131 - 201 4.9e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 139 - 262 3.2e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 870
Acc65I GGTACC 1 cut(s) 572
AccB1I GGYRCC 1 cut(s) 572
AciI CCGC 2 cut(s) 243, 738
AclWI GGATC 1 cut(s) 593
AcsI RAATTY 1 cut(s) 95
AcyI GRCGYC 1 cut(s) 867
AfaI GTAC 7 cut(s) 90, 163, 206, 218, 282, 478, 574
AfiI CCNNNNNNNGG 3 cut(s) 292, 582, 629
AgsI TTSAA 3 cut(s) 100, 640, 835
AjiI CACGTC 1 cut(s) 427
AluBI AGCT 6 cut(s) 140, 521, 655, 691, 731, 915
AluI AGCT 6 cut(s) 140, 521, 655, 691, 731, 915
Alw21I GWGCWC 1 cut(s) 673
Alw26I GTCTC 3 cut(s) 102, 648, 752
Alw44I GTGCAC 1 cut(s) 669
AlwI GGATC 1 cut(s) 593
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 2 cut(s) 406, 421
ApaLI GTGCAC 1 cut(s) 669
ApeKI GCWGC 3 cut(s) 317, 563, 782
ApoI RAATTY 1 cut(s) 95
ArsI GACNNNNNNTTYG 2 cut(s) 852, 884
Asp718I GGTACC 1 cut(s) 572
AspA2I CCTAGG 2 cut(s) 409, 514
AspLEI GCGC 1 cut(s) 852
AsuC2I CCSGG 4 cut(s) 45, 46, 293, 384
AsuHPI GGTGA 1 cut(s) 74
AsuNHI GCTAGC 1 cut(s) 911
AvaI CYCGRG 1 cut(s) 44
AvrII CCTAGG 2 cut(s) 409, 514
BaeGI GKGCMC 1 cut(s) 673
BanI GGYRCC 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 673
BbvI GCAGC 3 cut(s) 304, 550, 794
BccI CCATC 3 cut(s) 177, 575, 718
BciVI GTATCC 1 cut(s) 299
BclI TGATCA 2 cut(s) 471, 771
BcnI CCSGG 4 cut(s) 45, 46, 293, 384
BcoDI GTCTC 3 cut(s) 102, 648, 752
BfaI CTAG 3 cut(s) 410, 515, 912
BfmI CTRYAG 1 cut(s) 349
BfoI RGCGCY 1 cut(s) 853
BfuI GTATCC 1 cut(s) 299
BisI GCNGC 4 cut(s) 318, 564, 739, 783
BlnI CCTAGG 2 cut(s) 409, 514
BlsI GCNGC 4 cut(s) 319, 565, 740, 784
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 4 cut(s) 45, 46, 293, 384
BmeT110I CYCGRG 1 cut(s) 44
BmgBI CACGTC 1 cut(s) 427
BmiI GGNNCC 3 cut(s) 42, 229, 574
BmrFI CCNGG 4 cut(s) 45, 46, 293, 384
BmsI GCATC 1 cut(s) 116
BmtI GCTAGC 1 cut(s) 915
BpuEI CTTGAG 2 cut(s) 194, 707
BpuMI CCSGG 4 cut(s) 45, 46, 293, 384
BsaAI YACGTR 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 867
BsaJI CCNNGG 6 cut(s) 44, 409, 444, 514, 576, 843
BsaWI WCCGGW 1 cut(s) 694
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 447, 477
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 582, 629
Bse3DI GCAATG 2 cut(s) 91, 456
BseDI CCNNGG 6 cut(s) 44, 409, 444, 514, 576, 843
BseGI GGATG 3 cut(s) 131, 586, 610
BseLI CCNNNNNNNGG 3 cut(s) 292, 582, 629
BseMI GCAATG 2 cut(s) 91, 456
BseRI GAGGAG 1 cut(s) 312
BseSI GKGCMC 1 cut(s) 673
BseXI GCAGC 3 cut(s) 304, 550, 794
BsgI GTGCAG 1 cut(s) 588
BshFI GGCC 2 cut(s) 408, 423
BshNI GGYRCC 1 cut(s) 572
BsiHKAI GWGCWC 1 cut(s) 673
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 5 cut(s) 45, 293, 336, 384, 695
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 3 cut(s) 292, 582, 629
BsmAI GTCTC 3 cut(s) 102, 648, 752
BsmFI GGGAC 1 cut(s) 466
BsnI GGCC 2 cut(s) 408, 423
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 673
Bsp1407I TGTACA 1 cut(s) 88
Bsp143I GATC 4 cut(s) 146, 471, 585, 771
Bsp19I CCATGG 2 cut(s) 576, 843
BspACI CCGC 2 cut(s) 243, 738
BspANI GGCC 2 cut(s) 408, 423
BspLI GGNNCC 3 cut(s) 42, 229, 574
BspOI GCTAGC 1 cut(s) 915
BspPI GGATC 1 cut(s) 593
BspT107I GGYRCC 1 cut(s) 572
BsrDI GCAATG 2 cut(s) 91, 456
BsrGI TGTACA 1 cut(s) 88
BssECI CCNNGG 6 cut(s) 44, 409, 444, 514, 576, 843
BssMI GATC 4 cut(s) 146, 471, 585, 771
BssNI GRCGYC 1 cut(s) 867
BssT1I CCWWGG 5 cut(s) 409, 444, 514, 576, 843
Bst4CI ACNGT 4 cut(s) 209, 469, 598, 878
BstACI GRCGYC 1 cut(s) 867
BstAUI TGTACA 1 cut(s) 88
BstBAI YACGTR 1 cut(s) 204
BstC8I GCNNGC 4 cut(s) 142, 389, 561, 913
BstDSI CCRYGG 2 cut(s) 576, 843
BstF5I GGATG 3 cut(s) 131, 586, 610
BstH2I RGCGCY 1 cut(s) 853
BstHHI GCGC 1 cut(s) 852
BstKTI GATC 4 cut(s) 149, 474, 588, 774
BstMAI GTCTC 3 cut(s) 102, 648, 752
BstMBI GATC 4 cut(s) 146, 471, 585, 771
BstMWI GCNNNNNNNGC 3 cut(s) 388, 405, 688
BstNSI RCATGY 2 cut(s) 391, 395
BstSCI CCNGG 4 cut(s) 43, 44, 291, 382
BstSFI CTRYAG 1 cut(s) 349
BstSLI GKGCMC 1 cut(s) 673
BstV1I GCAGC 3 cut(s) 304, 550, 794
BstXI CCANNNNNNTGG 1 cut(s) 451
BsuI GTATCC 1 cut(s) 299
BsuRI GGCC 2 cut(s) 408, 423
BtgI CCRYGG 2 cut(s) 576, 843
BtrI CACGTC 1 cut(s) 427
BtsCI GGATG 3 cut(s) 131, 586, 610
BtsI GCAGTG 2 cut(s) 438, 837
BtsIMutI CAGTG 5 cut(s) 214, 438, 474, 837, 902
Cac8I GCNNGC 4 cut(s) 142, 389, 561, 913
CfoI GCGC 1 cut(s) 852
Cfr9I CCCGGG 1 cut(s) 44
Csp6I GTAC 7 cut(s) 89, 162, 205, 217, 281, 477, 573
CviAII CATG 8 cut(s) 50, 388, 392, 548, 577, 647, 712, 844
CviQI GTAC 7 cut(s) 89, 162, 205, 217, 281, 477, 573
DpnI GATC 4 cut(s) 148, 473, 587, 773
DpnII GATC 4 cut(s) 146, 471, 585, 771
Eco130I CCWWGG 5 cut(s) 409, 444, 514, 576, 843
Eco88I CYCGRG 1 cut(s) 44
EcoT14I CCWWGG 5 cut(s) 409, 444, 514, 576, 843
EcoT22I ATGCAT 2 cut(s) 131, 393
ErhI CCWWGG 5 cut(s) 409, 444, 514, 576, 843
FaeI CATG 8 cut(s) 53, 391, 395, 551, 580, 650, 715, 847
FaqI GGGAC 1 cut(s) 466
FatI CATG 8 cut(s) 49, 387, 391, 547, 576, 646, 711, 843
FbaI TGATCA 2 cut(s) 471, 771
Fnu4HI GCNGC 4 cut(s) 318, 564, 739, 783
FokI GGATG 3 cut(s) 138, 593, 597
Fsp4HI GCNGC 4 cut(s) 318, 564, 739, 783
FspBI CTAG 3 cut(s) 410, 515, 912
GlaI GCGC 1 cut(s) 851
GluI GCNGC 4 cut(s) 318, 564, 739, 783
HaeII RGCGCY 1 cut(s) 853
HaeIII GGCC 2 cut(s) 408, 423
HapII CCGG 5 cut(s) 45, 293, 336, 384, 695
HhaI GCGC 1 cut(s) 852
Hin1I GRCGYC 1 cut(s) 867
Hin1II CATG 8 cut(s) 53, 391, 395, 551, 580, 650, 715, 847
Hin6I GCGC 1 cut(s) 850
HinP1I GCGC 1 cut(s) 850
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 2 cut(s) 339, 524
HpaII CCGG 5 cut(s) 45, 293, 336, 384, 695
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 1 cut(s) 671
Hpy188I TCNGA 2 cut(s) 253, 872
Hpy188III TCNNGA 4 cut(s) 7, 173, 542, 686
Hpy8I GTNNAC 1 cut(s) 671
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 5 cut(s) 454, 487, 711, 748, 901
HpyCH4III ACNGT 4 cut(s) 209, 469, 598, 878
HpyCH4IV ACGT 4 cut(s) 203, 426, 479, 867
HpyCH4V TGCA 7 cut(s) 129, 266, 391, 559, 605, 671, 782
HpyF10VI GCNNNNNNNGC 3 cut(s) 388, 405, 688
HpySE526I ACGT 4 cut(s) 203, 426, 479, 867
Hsp92I GRCGYC 1 cut(s) 867
Hsp92II CATG 8 cut(s) 53, 391, 395, 551, 580, 650, 715, 847
HspAI GCGC 1 cut(s) 850
KpnI GGTACC 1 cut(s) 576
Ksp22I TGATCA 2 cut(s) 471, 771
Kzo9I GATC 4 cut(s) 146, 471, 585, 771
LmnI GCTCC 5 cut(s) 81, 137, 325, 518, 847
LpnPI CCDG 9 cut(s) 23, 58, 306, 349, 397, 545, 687, 708, 717
Lsp1109I GCAGC 3 cut(s) 304, 550, 794
LweI GCATC 1 cut(s) 116
MaeI CTAG 3 cut(s) 410, 515, 912
MaeII ACGT 4 cut(s) 203, 426, 479, 867
MaeIII GTNAC 4 cut(s) 67, 245, 463, 542
MalI GATC 4 cut(s) 148, 473, 587, 773
MboI GATC 4 cut(s) 146, 471, 585, 771
MboII GAAGA 1 cut(s) 733
MfeI CAATTG 1 cut(s) 822
MhlI GDGCHC 1 cut(s) 673
MluCI AATT 3 cut(s) 95, 775, 822
MmeI TCCRAC 2 cut(s) 85, 103
MnlI CCTC 7 cut(s) 45, 333, 490, 624, 693, 740, 805
Mph1103I ATGCAT 2 cut(s) 131, 393
MseI TTAA 2 cut(s) 507, 919
MslI CAYNNNNRTG 2 cut(s) 392, 842
MspI CCGG 5 cut(s) 45, 293, 336, 384, 695
MspR9I CCNGG 4 cut(s) 45, 46, 293, 384
MunI CAATTG 1 cut(s) 822
MwoI GCNNNNNNNGC 3 cut(s) 388, 405, 688
NciI CCSGG 4 cut(s) 45, 46, 293, 384
NcoI CCATGG 2 cut(s) 576, 843
NdeII GATC 4 cut(s) 146, 471, 585, 771
NheI GCTAGC 1 cut(s) 911
NlaIII CATG 8 cut(s) 53, 391, 395, 551, 580, 650, 715, 847
NlaIV GGNNCC 3 cut(s) 42, 229, 574
NmuCI GTSAC 2 cut(s) 67, 542
NsiI ATGCAT 2 cut(s) 131, 393
NspI RCATGY 2 cut(s) 391, 395
PaeI GCATGC 1 cut(s) 391
PfeI GAWTC 2 cut(s) 339, 524
PflFI GACNNNGTC 1 cut(s) 113
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 4 cut(s) 319, 565, 740, 784
Ppu21I YACGTR 1 cut(s) 204
PspN4I GGNNCC 3 cut(s) 42, 229, 574
PsrI GAACNNNNNNTAC 2 cut(s) 264, 296
PsyI GACNNNGTC 1 cut(s) 113
RsaI GTAC 7 cut(s) 90, 163, 206, 218, 282, 478, 574
RsaNI GTAC 7 cut(s) 89, 162, 205, 217, 281, 477, 573
RseI CAYNNNNRTG 2 cut(s) 392, 842
SaqAI TTAA 2 cut(s) 507, 919
SatI GCNGC 4 cut(s) 318, 564, 739, 783
Sau3AI GATC 4 cut(s) 146, 471, 585, 771
ScaI AGTACT 1 cut(s) 218
ScrFI CCNGG 4 cut(s) 45, 46, 293, 384
SduI GDGCHC 1 cut(s) 673
SfaNI GCATC 1 cut(s) 116
SfcI CTRYAG 1 cut(s) 349
SmaI CCCGGG 1 cut(s) 46
SmiMI CAYNNNNRTG 2 cut(s) 392, 842
SmlI CTYRAG 2 cut(s) 173, 686
SmoI CTYRAG 2 cut(s) 173, 686
SphI GCATGC 1 cut(s) 391
Sse9I AATT 3 cut(s) 95, 775, 822
SsiI CCGC 2 cut(s) 243, 738
SspMI CTAG 3 cut(s) 410, 515, 912
StyD4I CCNGG 4 cut(s) 43, 44, 291, 382
StyI CCWWGG 5 cut(s) 409, 444, 514, 576, 843
TaaI ACNGT 4 cut(s) 209, 469, 598, 878
TaiI ACGT 4 cut(s) 206, 429, 482, 870
TasI AATT 3 cut(s) 95, 775, 822
TatI WGTACW 2 cut(s) 88, 216
TauI GCSGC 1 cut(s) 741
TfiI GAWTC 2 cut(s) 339, 524
Tru1I TTAA 2 cut(s) 507, 919
Tru9I TTAA 2 cut(s) 507, 919
TscAI CASTG 5 cut(s) 214, 438, 474, 844, 909
TseFI GTSAC 2 cut(s) 67, 542
TseI GCWGC 3 cut(s) 317, 563, 782
Tsp45I GTSAC 2 cut(s) 67, 542
TspDTI ATGAA 3 cut(s) 27, 285, 767
TspGWI ACGGA 3 cut(s) 86, 240, 275
TspMI CCCGGG 1 cut(s) 44
TspRI CASTG 5 cut(s) 214, 438, 474, 844, 909
Tth111I GACNNNGTC 1 cut(s) 113
VneI GTGCAC 1 cut(s) 669
XapI RAATTY 1 cut(s) 95
XceI RCATGY 2 cut(s) 391, 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmaI CCCGGG 1 cut(s) 44
XmaJI CCTAGG 2 cut(s) 409, 514
XspI CTAG 3 cut(s) 410, 515, 912
ZraI GACGTC 1 cut(s) 868
ZrmI AGTACT 1 cut(s) 218
Zsp2I ATGCAT 2 cut(s) 131, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.