Rorug06G0169800

Protein of unknown function (DUF3685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003845
Physical Location & Seq
Reverse (-)
25068076 .. 25070298
2223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0169800.1

Sequence Viewer

Length: 408 bp
ATGGCTCGCCGCAGCTCAGGAGGTCGATCTGCCCGTCCAGCTCCTCGTCGTGCACCAGCCCCTGCACCAGTTCGCCAGGCTCCTGCTCCAGCTCCTGCTCAGAGTGGAAGCGGTGGGGGTATTGGATCAGTCATTGCTGAGGGAATGGCTTTCGGCGGTGGAAGCGCCATTGCACACAGAGCTGTGGATGGTTTGTTTGGTCCTCGCACTATTAAACATGAAACTGTAGTCTCTGCTGCTCCAACACCTACTGCTAACAGTATCGGTGGCTCTGATGCTTGCAGTGTTCACTCCAAGGCATTCCAAGATTGTGTCAACAACTTTGGAAGTGATATCAGCAAGTGCCAGTTCTACATGGATATGCTCTCTGAGTGCAAGAAGAACAACTCTGGCATGCTGAGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3685

Protein Analysis

135

Amino Acids

13.62

Weight (kDa)

9.61

Isoelectric Point (pI)

69.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHCH PF06747 94 - 128 9.6e-09 CHCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 10, 111, 156
AclWI GGATC 1 cut(s) 133
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 4 cut(s) 15, 41, 92, 182
AluI AGCT 4 cut(s) 15, 41, 92, 182
Alw21I GWGCWC 1 cut(s) 55
Alw26I GTCTC 1 cut(s) 235
Alw44I GTGCAC 1 cut(s) 51
AlwI GGATC 1 cut(s) 133
AlwNI CAGNNNCTG 2 cut(s) 62, 95
ApaLI GTGCAC 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 12, 236
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 200
AvaII GGWCC 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 55
Bbv12I GWGCWC 1 cut(s) 55
BbvCI CCTCAGC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 24, 223
BccI CCATC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 235
BfmI CTRYAG 1 cut(s) 225
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 3 cut(s) 10, 13, 237
BlsI GCNGC 3 cut(s) 11, 14, 238
Bme1390I CCNGG 1 cut(s) 77
Bme18I GGWCC 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 265
BpmI CTGGAG 1 cut(s) 72
Bpu10I CCTNAGC 2 cut(s) 16, 138
BsaJI CCNNGG 1 cut(s) 294
Bse1I ACTGG 2 cut(s) 68, 346
Bse3DI GCAATG 2 cut(s) 132, 168
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 193
BseMI GCAATG 2 cut(s) 132, 168
BseMII CTCAG 5 cut(s) 30, 113, 129, 360, 389
BseNI ACTGG 2 cut(s) 68, 346
BseRI GAGGAG 1 cut(s) 33
BseSI GKGCMC 1 cut(s) 55
BseXI GCAGC 2 cut(s) 24, 223
BsgI GTGCAG 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 235
BsmI GAATGC 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 55
Bsp143I GATC 2 cut(s) 26, 125
BspACI CCGC 3 cut(s) 10, 111, 156
BspCNI CTCAG 5 cut(s) 29, 112, 130, 361, 390
BspLI GGNNCC 1 cut(s) 81
BspPI GGATC 1 cut(s) 133
BsrDI GCAATG 2 cut(s) 132, 168
BsrI ACTGG 2 cut(s) 68, 346
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 2 cut(s) 26, 125
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 2 cut(s) 226, 260
BstC8I GCNNGC 3 cut(s) 7, 280, 395
BstDEI CTNAG 5 cut(s) 16, 99, 138, 369, 398
BstF5I GGATG 1 cut(s) 193
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 2 cut(s) 29, 128
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 2 cut(s) 26, 125
BstMWI GCNNNNNNNGC 3 cut(s) 38, 162, 179
BstNI CCWGG 1 cut(s) 77
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 1 cut(s) 225
BstSLI GKGCMC 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 24, 223
BtsCI GGATG 1 cut(s) 193
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 289
Cac8I GCNNGC 3 cut(s) 7, 280, 395
CaiI CAGNNNCTG 2 cut(s) 62, 95
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 200
CviAII CATG 3 cut(s) 218, 355, 394
CviJI RGCY 9 cut(s) 5, 15, 41, 59, 80, 92, 149, 182, 270
CviKI_1 RGCY 9 cut(s) 5, 15, 41, 59, 80, 92, 149, 182, 270
DdeI CTNAG 5 cut(s) 16, 99, 138, 369, 398
DpnI GATC 2 cut(s) 28, 127
DpnII GATC 2 cut(s) 26, 125
Eco130I CCWWGG 1 cut(s) 294
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 75
EcoRV GATATC 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 3 cut(s) 221, 358, 397
FaiI YATR 4 cut(s) 219, 356, 362, 395
FatI CATG 3 cut(s) 217, 354, 393
Fnu4HI GCNGC 3 cut(s) 10, 13, 237
FokI GGATG 1 cut(s) 200
Fsp4HI GCNGC 3 cut(s) 10, 13, 237
GlaI GCGC 1 cut(s) 166
GluI GCNGC 3 cut(s) 10, 13, 237
GsuI CTGGAG 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 168
HhaI GCGC 1 cut(s) 167
Hin1II CATG 3 cut(s) 221, 358, 397
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
Hpy166II GTNNAC 3 cut(s) 53, 289, 316
Hpy188I TCNGA 3 cut(s) 102, 274, 370
Hpy188III TCNNGA 1 cut(s) 18
Hpy8I GTNNAC 3 cut(s) 53, 289, 316
Hpy99I CGWCG 1 cut(s) 51
HpyCH4III ACNGT 2 cut(s) 226, 260
HpyCH4V TGCA 5 cut(s) 53, 65, 173, 282, 375
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 162, 179
HpyF3I CTNAG 5 cut(s) 16, 99, 138, 369, 398
Hsp92II CATG 3 cut(s) 221, 358, 397
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 2 cut(s) 26, 125
LmnI GCTCC 5 cut(s) 46, 85, 91, 97, 244
Lsp1109I GCAGC 2 cut(s) 24, 223
LweI GCATC 1 cut(s) 265
MalI GATC 2 cut(s) 28, 127
MboI GATC 2 cut(s) 26, 125
MboII GAAGA 1 cut(s) 391
MhlI GDGCHC 1 cut(s) 55
MmeI TCCRAC 1 cut(s) 266
MnlI CCTC 4 cut(s) 14, 54, 133, 213
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 359
MspR9I CCNGG 1 cut(s) 77
Mva1269I GAATGC 1 cut(s) 299
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 3 cut(s) 38, 162, 179
NdeII GATC 2 cut(s) 26, 125
NlaIII CATG 3 cut(s) 221, 358, 397
NlaIV GGNNCC 1 cut(s) 81
NspI RCATGY 1 cut(s) 397
PaeI GCATGC 1 cut(s) 397
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PctI GAATGC 1 cut(s) 299
PkrI GCNGC 3 cut(s) 11, 14, 238
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 1 cut(s) 200
PstNI CAGNNNCTG 2 cut(s) 62, 95
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 3 cut(s) 10, 13, 237
Sau3AI GATC 2 cut(s) 26, 125
Sau96I GGNCC 1 cut(s) 200
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 1 cut(s) 55
SetI ASST 6 cut(s) 17, 25, 43, 94, 184, 250
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 1 cut(s) 200
SmiMI CAYNNNNRTG 1 cut(s) 359
SphI GCATGC 1 cut(s) 397
SsiI CCGC 3 cut(s) 10, 111, 156
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 226, 260
TaqI TCGA 1 cut(s) 25
TauI GCSGC 1 cut(s) 12
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 289
TseI GCWGC 2 cut(s) 12, 236
TspDTI ATGAA 1 cut(s) 234
TspRI CASTG 1 cut(s) 289
VneI GTGCAC 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 200
XceI RCATGY 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.