Rh5DG385300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018810
Physical Location & Seq
Reverse (-)
59285079 .. 59325861
40783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG385300.1

Sequence Viewer

Length: 435 bp
ATGAAAAATCCTTTCACAGGGAGAAGACTGAACATAGAGAGGACTTGGATAGGTCTGCAGGGTTTGTGGATCGAGTACATCTCTTCCGTGGCTGCTCAGATATTACCAGATTCCGTCTTCAATAACATTCAATGCAGAAGTGTTATTCATGACAAAGGTGACTTGGTCCATGAATGGTGTGCACCGGAATCTGTGCATATATGTTTGTTGTCATGCCTCAAGAGAATTTGCATACGGGGATTTGAAGGACGGCCAGATAAGATGGAAGTAGCAGGGTACTTATTGAAGCACGGAGTTCTTTTGAGTAAGGTGACAGTCTATACTTGTAGTTTCCTTAAAGAATTTGTGGCAGAGTTCTTCCTTGAATTTTCAAATTTCCTGAGAGGTTCAAAGACTTGTCACATTGAATGGATACTGGGGACGACCAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

144

Amino Acids

16.52

Weight (kDa)

8.5

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBD PF08387 62 - 105 1.3e-10 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018810)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g02890 FvH4_3g02890
rosa_multiflora Rmu_sc0004404.1_g000001
rosa_samantha Rh3DG092100 Rh5AG360600 Rh5BG371600 Rh5CG393800 Rh5DG385300 Rh5DG385400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 4 cut(s) 225, 341, 365, 373
AfaI GTAC 2 cut(s) 77, 278
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 8 cut(s) 121, 131, 245, 286, 365, 372, 390, 407
Alw21I GWGCWC 1 cut(s) 184
Alw44I GTGCAC 1 cut(s) 180
AlwI GGATC 1 cut(s) 77
AoxI GGCC 1 cut(s) 251
ApaLI GTGCAC 1 cut(s) 180
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 4 cut(s) 225, 341, 365, 373
AspS9I GGNCC 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 170, 322
AvaII GGWCC 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 184
BbsI GAAGAC 2 cut(s) 31, 109
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 79
BccI CCATC 1 cut(s) 256
BceAI ACGGC 1 cut(s) 266
BciVI GTATCC 1 cut(s) 405
BfmI CTRYAG 1 cut(s) 56
BfuI GTATCC 1 cut(s) 405
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 166
BmrI ACTGGG 1 cut(s) 425
BmuI ACTGGG 1 cut(s) 425
BpiI GAAGAC 2 cut(s) 31, 109
BplI GAGNNNNNCTC 2 cut(s) 65, 97
BpuEI CTTGAG 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 184
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 420
BseDI CCNNGG 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 2 cut(s) 110, 371
BseNI ACTGG 1 cut(s) 420
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 1 cut(s) 79
BshFI GGCC 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 1 cut(s) 185
BslI CCNNNNNNNGG 1 cut(s) 17
BsnI GGCC 1 cut(s) 253
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 1 cut(s) 69
BspANI GGCC 1 cut(s) 253
BspCNI CTCAG 2 cut(s) 109, 372
BspHI TCATGA 1 cut(s) 148
BspMAI CTGCAG 1 cut(s) 60
BspPI GGATC 1 cut(s) 77
BsrI ACTGG 1 cut(s) 420
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 316
Bst6I CTCTTC 1 cut(s) 88
BstDEI CTNAG 2 cut(s) 96, 380
BstDSI CCRYGG 1 cut(s) 87
BstENI CCTNNNNNAGG 1 cut(s) 15
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstSFI CTRYAG 1 cut(s) 56
BstSLI GKGCMC 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 2 cut(s) 31, 109
BsuI GTATCC 1 cut(s) 405
BsuRI GGCC 1 cut(s) 253
BtgI CCRYGG 1 cut(s) 87
CciI TCATGA 1 cut(s) 148
Cfr13I GGNCC 1 cut(s) 166
Csp6I GTAC 2 cut(s) 76, 277
CviAII CATG 3 cut(s) 149, 170, 213
CviJI RGCY 2 cut(s) 92, 253
CviKI_1 RGCY 2 cut(s) 92, 253
CviQI GTAC 2 cut(s) 76, 277
DdeI CTNAG 2 cut(s) 96, 380
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 15
FaeI CATG 3 cut(s) 152, 173, 216
FaiI YATR 9 cut(s) 35, 150, 171, 198, 200, 202, 214, 233, 321
FatI CATG 3 cut(s) 148, 169, 212
Fnu4HI GCNGC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 93
GluI GCNGC 1 cut(s) 93
HaeIII GGCC 1 cut(s) 253
HapII CCGG 1 cut(s) 185
Hin1II CATG 3 cut(s) 152, 173, 216
HinfI GANTC 2 cut(s) 110, 188
HpaII CCGG 1 cut(s) 185
HphI GGTGA 2 cut(s) 170, 322
Hpy166II GTNNAC 1 cut(s) 182
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 3 cut(s) 149, 220, 379
Hpy8I GTNNAC 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4V TGCA 5 cut(s) 58, 135, 182, 196, 231
HpyF3I CTNAG 2 cut(s) 96, 380
Hsp92II CATG 3 cut(s) 152, 173, 216
Kzo9I GATC 1 cut(s) 69
LpnPI CCDG 8 cut(s) 3, 44, 120, 198, 258, 267, 392, 401
Lsp1109I GCAGC 1 cut(s) 79
MaeIII GTNAC 3 cut(s) 158, 310, 398
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 4 cut(s) 36, 75, 109, 349
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 4 cut(s) 225, 341, 365, 373
MnlI CCTC 3 cut(s) 33, 227, 377
MseI TTAA 1 cut(s) 336
MspI CCGG 1 cut(s) 185
NdeII GATC 1 cut(s) 69
NlaIII CATG 3 cut(s) 152, 173, 216
NmuCI GTSAC 3 cut(s) 158, 310, 398
PagI TCATGA 1 cut(s) 148
PfeI GAWTC 2 cut(s) 110, 188
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 1 cut(s) 94
PspPI GGNCC 1 cut(s) 166
PstI CTGCAG 1 cut(s) 60
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 2 cut(s) 77, 278
RsaNI GTAC 2 cut(s) 76, 277
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 1 cut(s) 166
SduI GDGCHC 1 cut(s) 184
SetI ASST 4 cut(s) 55, 160, 312, 388
SfcI CTRYAG 1 cut(s) 56
SinI GGWCC 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 4 cut(s) 225, 341, 365, 373
TaaI ACNGT 1 cut(s) 316
TaqI TCGA 1 cut(s) 72
TasI AATT 4 cut(s) 225, 341, 365, 373
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 2 cut(s) 110, 188
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TseFI GTSAC 3 cut(s) 158, 310, 398
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 3 cut(s) 158, 310, 398
TspDTI ATGAA 3 cut(s) 17, 137, 186
TspGWI ACGGA 3 cut(s) 76, 103, 306
Tth111I GACNNNGTC 1 cut(s) 164
VneI GTGCAC 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 166
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 4 cut(s) 225, 341, 365, 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.