Rh6CG119900

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0018771
Physical Location & Seq
Forward (+)
13987934 .. 13988230
297 bp
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UTR
Exon/CDS
Intron
Rh6CG119900.1

Sequence Viewer

Length: 297 bp
ATGAGGAGAGCAAGACATATTCGGCATAAGCTTGAGCAGCTCTTGGAGAAGATCGGCATAGAGCTCACCTCAAATCCAGTTGATTTGGAGCCTATAGAGAAGGCTATTACCACCGGTTTCTTACCTCATTCTGCAAAGCAGCAAATGAATGGATCTTATAGAACAGTCAAAGAGCCAGATTCACCAACTATCCGTATACATCCCAACTCGGGGATGGCACAAGTGCTCCCGACGTGGGTTGTGTATCATGATTTGGTGCCCACAACCGGAGAATATATGAGACAGGTAACACTGTAA

Protein Analysis

98

Amino Acids

11.21

Weight (kDa)

9.16

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_NTP_bind PF07717 32 - 97 1.7e-13 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018771)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06762
rosa_laevigata RLG00000016199
rosa_multiflora Rmu_sc0002270.1_g000026
rosa_rugosa Rorug02G0015300
rosa_samantha Rh2BG073600 Rh2DG060200 Rh2DG071700 Rh6BG121500 Rh6CG119900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 256
AccI GTMKAC 1 cut(s) 196
AclWI GGATC 1 cut(s) 160
AfiI CCNNNNNNNGG 4 cut(s) 209, 210, 235, 266
AgeI ACCGGT 1 cut(s) 113
AjiI CACGTC 1 cut(s) 234
AluBI AGCT 3 cut(s) 31, 40, 64
AluI AGCT 3 cut(s) 31, 40, 64
Alw21I GWGCWC 2 cut(s) 66, 228
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 1 cut(s) 160
Ama87I CYCGRG 1 cut(s) 208
ApeKI GCWGC 2 cut(s) 37, 139
AsiGI ACCGGT 1 cut(s) 113
AsuHPI GGTGA 2 cut(s) 58, 174
AvaI CYCGRG 1 cut(s) 208
BaeGI GKGCMC 1 cut(s) 261
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 2 cut(s) 66, 228
BbvI GCAGC 2 cut(s) 49, 151
BccI CCATC 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 274
BfmI CTRYAG 1 cut(s) 93
BisI GCNGC 2 cut(s) 38, 140
BlsI GCNGC 2 cut(s) 39, 141
BmeT110I CYCGRG 1 cut(s) 208
BmgBI CACGTC 1 cut(s) 234
BmiI GGNNCC 2 cut(s) 90, 258
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpuEI CTTGAG 1 cut(s) 53
BsaWI WCCGGW 2 cut(s) 113, 266
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 210, 235, 266
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 77
BseGI GGATG 2 cut(s) 199, 219
BseLI CCNNNNNNNGG 4 cut(s) 209, 210, 235, 266
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 2 cut(s) 49, 151
BshNI GGYRCC 1 cut(s) 256
BshTI ACCGGT 1 cut(s) 113
BsiHKAI GWGCWC 2 cut(s) 66, 228
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 2 cut(s) 114, 267
BslI CCNNNNNNNGG 4 cut(s) 209, 210, 235, 266
BsmAI GTCTC 1 cut(s) 274
BsoBI CYCGRG 1 cut(s) 208
Bsp1286I GDGCHC 3 cut(s) 66, 228, 261
Bsp143I GATC 2 cut(s) 51, 152
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 90, 258
BspPI GGATC 1 cut(s) 160
BspT107I GGYRCC 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 1 cut(s) 77
BssAI RCCGGY 1 cut(s) 113
BssMI GATC 2 cut(s) 51, 152
BssNAI GTATAC 1 cut(s) 197
Bst1107I GTATAC 1 cut(s) 197
Bst4CI ACNGT 2 cut(s) 166, 294
BstF5I GGATG 2 cut(s) 199, 219
BstKTI GATC 2 cut(s) 54, 155
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 2 cut(s) 51, 152
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 261
BstV1I GCAGC 2 cut(s) 49, 151
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BstZ17I GTATAC 1 cut(s) 197
BtrI CACGTC 1 cut(s) 234
BtsCI GGATG 2 cut(s) 199, 219
BtsIMutI CAGTG 1 cut(s) 290
CciI TCATGA 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 113
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 1 cut(s) 248
CviJI RGCY 6 cut(s) 31, 40, 64, 91, 104, 175
CviKI_1 RGCY 6 cut(s) 31, 40, 64, 91, 104, 175
DpnI GATC 2 cut(s) 53, 154
DpnII GATC 2 cut(s) 51, 152
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 1 cut(s) 251
FaiI YATR 9 cut(s) 18, 27, 59, 95, 159, 197, 249, 276, 278
FatI CATG 1 cut(s) 247
FblI GTMKAC 1 cut(s) 196
Fnu4HI GCNGC 2 cut(s) 38, 140
FokI GGATG 2 cut(s) 186, 226
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 2 cut(s) 38, 140
GluI GCNGC 2 cut(s) 38, 140
HapII CCGG 2 cut(s) 114, 267
Hin1II CATG 1 cut(s) 251
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 1 cut(s) 179
HpaII CCGG 2 cut(s) 114, 267
HphI GGTGA 2 cut(s) 58, 174
Hpy166II GTNNAC 1 cut(s) 197
Hpy188III TCNNGA 2 cut(s) 229, 248
Hpy8I GTNNAC 1 cut(s) 197
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 166, 294
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpySE526I ACGT 1 cut(s) 233
Hsp92II CATG 1 cut(s) 251
Kzo9I GATC 2 cut(s) 51, 152
LmnI GCTCC 2 cut(s) 88, 231
LpnPI CCDG 5 cut(s) 90, 127, 189, 269, 280
Lsp1109I GCAGC 2 cut(s) 49, 151
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 1 cut(s) 286
MalI GATC 2 cut(s) 53, 154
MboI GATC 2 cut(s) 51, 152
MboII GAAGA 1 cut(s) 61
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 3 cut(s) 66, 228, 261
MnlI CCTC 2 cut(s) 79, 135
MspI CCGG 2 cut(s) 114, 267
MwoI GCNNNNNNNGC 1 cut(s) 37
NdeII GATC 2 cut(s) 51, 152
NlaIII CATG 1 cut(s) 251
NlaIV GGNNCC 2 cut(s) 90, 258
PagI TCATGA 1 cut(s) 247
PfeI GAWTC 1 cut(s) 179
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 2 cut(s) 39, 141
Psp124BI GAGCTC 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 90, 258
PsuI RGATCY 1 cut(s) 152
SacI GAGCTC 1 cut(s) 66
SatI GCNGC 2 cut(s) 38, 140
Sau3AI GATC 2 cut(s) 51, 152
SduI GDGCHC 3 cut(s) 66, 228, 261
SetI ASST 7 cut(s) 33, 42, 66, 71, 127, 236, 288
SfcI CTRYAG 1 cut(s) 93
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SstI GAGCTC 1 cut(s) 66
TaaI ACNGT 2 cut(s) 166, 294
TaiI ACGT 1 cut(s) 236
TfiI GAWTC 1 cut(s) 179
TscAI CASTG 1 cut(s) 297
TseI GCWGC 2 cut(s) 37, 139
TspDTI ATGAA 1 cut(s) 161
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 1 cut(s) 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.