Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0007341

Physical Location & Seq

Genomic Coordinates Forward (+)

66889738 .. 66891162

Gene Structure 1425 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG517700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGACATGCAGTCCCAAAACCACCAAAACCCTCCTCATCCCATTCCCGAGTCATCGCCACAACCCAGTTTTCCGGCCAAACTTGCCCACCAGCTGAAATTCAATCTCATTTTCAGGTCCAAATGGGCCGAGTTGAACGGTGCAATGGGCGACTTAGGCACCTACATCCCAATCGTAGTGGCCTTAACACTAGCCAGAGACTTGAACTTGGGCACCACCTTGATCTTCACCGGAATCTACAACATCATCACCGGAGCCATCTACGGCGTTCCCATGCCGGTCCAACCTATGAAGGCCATTGCCGCCACAGCCCTGGCCAACCCGGATTTCGGCATCCCCGAAATCATGGCCGCCGGAATCCTAACCGGTAGCACCCTCATCGTTTTGGGTGCCACCGGTTTGATGCAACTGGTCTATAAGTTGATCCCTTTGTGTGTGGTCAGGGGAGTTCAACTGGCACAGGGATTGTCATTTGCGTTAACCGCGGTCAAGTACATTAAGAAGGTGCAAAACTTGCCCAAATCCAAAGCCATGGGGGAGAGGCACTGGTTTGGGTTGGATGGTTTGGTGTTGGCTATTGTTTGTCTTTGTTTCATAGTCATTGTTAATGGTGCAGGTGAGGAATATCATCCAGTGAGGGAAGGTGAAGTTGAGGTTGAAGAAGAAAGACCTAGCAGAAAAAAGAGGTGGAGGAAAATCATAGCTTCACTCCCTTCGGCTTTTTTAATTTTTGTGTTGGGTGTGATTCTGGCTTTTATAAGAAAGCCAAAAGTTGTGCATGAAATTAAATTCGGGCCATCTCCTATGGAGGTTGTGAAGATTAGTAGGCATGCATGGAAAGATGGGTTCATCAAAGGGACAATTCCACAACTGCCCCTGTCAATATTGAACTCAGTGATTGCTGTGTGCAAATTCTCCTCTGATCTTTTCCCAGATAAAGATTTCTCTCCCACATCAATTTCGATCACGGTTGGGCTGATGAATGTGGTGGGTAGTTGGTTCGGAGCCATGCCCAGTTGTCACGGGGCCGGCGGGTTGGCCGGCCAGTACAAGTTTGGGGGGCGGAGTGGGGGGTGTGTGGCACTACTAGGATCAGCCAAATTGGTGTTGGGTTTGGCTTTAGGGACTTCTCTGGTGACCATTTTGAACCAATTCCCAGTTGGGGTTTTGGGGGTGTTGCTCTTGTTTGCTGGGATTGAACTGGCTATGTGTGCTAGGGACATGCATACTAAAGAAGAGTCCTTTGTGATGCTTATTTGCACTGCTGTTTCACTAGTGGGTTCGAGTGCAGCACTAGGATTTGTGGTGGGGATGGGTGTGTATGTTCTACTTTGGCTGAGAAAGTTGGGCTGCAAAGACAAACCTAATTGGATGATATGGAAGCATGGTCGAACCATAGTAGAAAATTATGATCACAACCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005773 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008509 GO:0015075 GO:0015098 GO:0015103 GO:0015318 GO:0015689 GO:0015698 GO:0016020 GO:0022857 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098656

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_MOT1

Protein Analysis

474

Amino Acids

51.2

Weight (kDa)

9.29

Isoelectric Point (pI)

33.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_MOT1	PF16983	43 - 158	2.3e-26	Molybdate transporter of MFS superfamily
MFS_MOT1	PF16983	283 - 401	4e-35	Molybdate transporter of MFS superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007341)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25680
fragaria_vesca	FvH4_2g30230
malus_domestica	MD08G1135400.v1.1
prunus_persica	Prupe.1G465500_v2.0.a1
pyrus_communis	pycom08g11440
rosa_chinensis	RchiOBHm_Chr4g0397011 RchiOBHm_Chr6g0312651
rosa_laevigata	RLG00000009458 RLG00000009459 RLG00000010307
rosa_multiflora	Rmu_sc0002859.1_g000003 Rmu_sc0002963.1_g000005 Rmu_ssc0000412.1_g000007
rosa_roxburghii	Rroxscaffold_5G00342030
rosa_rugosa	Rorug03G0345500 Rorug06G0398100
rosa_samantha	Rh4AG076100 Rh4BG072600 Rh4CG080800 Rh4DG070100 Rh6AG515400 Rh6BG526700 Rh6DG517700
rosa_wichuraiana	Rw4G006110 Rw6G044780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	758
AarI	CACCTGC	1 cut(s)	605
Acc36I	ACCTGC	1 cut(s)	605
AccB1I	GGYRCC	3 cut(s)	158, 212, 389
AccII	CGCG	1 cut(s)	485
AciI	CCGC	6 cut(s)	303, 351, 483, 485, 1032, 1063
AclWI	GGATC	2 cut(s)	418, 1099
AcoI	YGGCCR	5 cut(s)	75, 315, 348, 1038, 1042
AcsI	RAATTY	3 cut(s)	98, 788, 911
AfaI	GTAC	2 cut(s)	494, 1049
AfiI	CCNNNNNNNGG	2 cut(s)	329, 1162
AgeI	ACCGGT	2 cut(s)	365, 395
AgsI	TTSAA	8 cut(s)	103, 136, 205, 452, 659, 889, 1147, 1199
AhdI	GACNNNNNGTC	1 cut(s)	10
AhlI	ACTAGT	1 cut(s)	1273
AjnI	CCWGG	1 cut(s)	312
AjuI	GAANNNNNNNTTGG	2 cut(s)	311, 343
AluBI	AGCT	2 cut(s)	94, 704
AluI	AGCT	2 cut(s)	94, 704
Alw26I	GTCTC	1 cut(s)	192
AlwI	GGATC	2 cut(s)	418, 1099
Ama87I	CYCGRG	1 cut(s)	47
ApeKI	GCWGC	2 cut(s)	1289, 1350
ApoI	RAATTY	3 cut(s)	98, 788, 911
AsiGI	ACCGGT	2 cut(s)	365, 395
Asp700I	GAANNNNTTC	1 cut(s)	1151
AspS9I	GGNCC	5 cut(s)	117, 126, 280, 794, 1026
AsuC2I	CCSGG	1 cut(s)	323
AsuHPI	GGTGA	5 cut(s)	220, 241, 629, 656, 1147
AvaI	CYCGRG	1 cut(s)	47
AvaII	GGWCC	2 cut(s)	117, 280
BaeGI	GKGCMC	1 cut(s)	215
BalI	TGGCCA	1 cut(s)	317
BanI	GGYRCC	3 cut(s)	158, 212, 389
BbvI	GCAGC	2 cut(s)	1301, 1337
BccI	CCATC	5 cut(s)	266, 554, 805, 836, 1306
BceAI	ACGGC	1 cut(s)	280
BcgI	CGANNNNNNTGC	2 cut(s)	361, 395
BciT130I	CCWGG	1 cut(s)	314
BclI	TGATCA	1 cut(s)	1411
BcnI	CCSGG	1 cut(s)	323
BcoDI	GTCTC	1 cut(s)	192
BcuI	ACTAGT	1 cut(s)	1273
BfaI	CTAG	6 cut(s)	191, 672, 1088, 1215, 1274, 1295
BfuAI	ACCTGC	1 cut(s)	605
BisI	GCNGC	4 cut(s)	303, 351, 1290, 1351
BlsI	GCNGC	4 cut(s)	304, 352, 1291, 1352
Bme1390I	CCNGG	2 cut(s)	314, 323
Bme18I	GGWCC	2 cut(s)	117, 280
BmeRI	GACNNNNNGTC	1 cut(s)	10
BmeT110I	CYCGRG	1 cut(s)	47
BmgT120I	GGNCC	5 cut(s)	117, 126, 280, 794, 1026
BmiI	GGNNCC	6 cut(s)	160, 214, 256, 391, 1006, 1027
BmrFI	CCNGG	2 cut(s)	314, 323
BmrI	ACTGGG	3 cut(s)	60, 1008, 1151
BmsI	GCATC	3 cut(s)	342, 393, 1239
BmuI	ACTGGG	3 cut(s)	60, 1008, 1151
BpuMI	CCSGG	1 cut(s)	323
BsaJI	CCNNGG	3 cut(s)	312, 483, 531
BsaWI	WCCGGW	4 cut(s)	230, 251, 365, 395
Bsc4I	CCNNNNNNNGG	2 cut(s)	329, 1162
Bse118I	RCCGGY	5 cut(s)	277, 365, 395, 1028, 1040
Bse1I	ACTGG	9 cut(s)	66, 414, 459, 551, 632, 1014, 1045, 1157, 1206
Bse3DI	GCAATG	2 cut(s)	150, 297
BseBI	CCWGG	1 cut(s)	314
BseDI	CCNNGG	3 cut(s)	312, 483, 531
BseGI	GGATG	7 cut(s)	37, 165, 333, 565, 628, 1317, 1377
BseLI	CCNNNNNNNGG	2 cut(s)	329, 1162
BseMI	GCAATG	2 cut(s)	150, 297
BseMII	CTCAG	2 cut(s)	906, 1328
BseNI	ACTGG	9 cut(s)	66, 414, 459, 551, 632, 1014, 1045, 1157, 1206
BseRI	GAGGAG	2 cut(s)	24, 907
BseSI	GKGCMC	1 cut(s)	215
BseXI	GCAGC	2 cut(s)	1301, 1337
BseYI	CCCAGC	1 cut(s)	1190
BsgI	GTGCAG	2 cut(s)	633, 1308
Bsh1236I	CGCG	1 cut(s)	485
BshNI	GGYRCC	3 cut(s)	158, 212, 389
BshTI	ACCGGT	2 cut(s)	365, 395
BsiHKCI	CYCGRG	1 cut(s)	47
BslFI	GGGAC	3 cut(s)	871, 1138, 1232
BslI	CCNNNNNNNGG	2 cut(s)	329, 1162
BsmAI	GTCTC	1 cut(s)	192
BsmFI	GGGAC	3 cut(s)	871, 1138, 1232
BsoBI	CYCGRG	1 cut(s)	47
Bsp1286I	GDGCHC	1 cut(s)	215
Bsp143I	GATC	6 cut(s)	222, 423, 922, 963, 1091, 1411
Bsp19I	CCATGG	1 cut(s)	531
BspACI	CCGC	6 cut(s)	303, 351, 483, 485, 1032, 1063
BspCNI	CTCAG	2 cut(s)	905, 1329
BspFNI	CGCG	1 cut(s)	485
BspLI	GGNNCC	6 cut(s)	160, 214, 256, 391, 1006, 1027
BspMI	ACCTGC	1 cut(s)	605
BspPI	GGATC	2 cut(s)	418, 1099
BspT107I	GGYRCC	3 cut(s)	158, 212, 389
BsrDI	GCAATG	2 cut(s)	150, 297
BsrFI	RCCGGY	5 cut(s)	277, 365, 395, 1028, 1040
BsrI	ACTGG	9 cut(s)	66, 414, 459, 551, 632, 1014, 1045, 1157, 1206
BssAI	RCCGGY	5 cut(s)	277, 365, 395, 1028, 1040
BssECI	CCNNGG	3 cut(s)	312, 483, 531
BssMI	GATC	6 cut(s)	222, 423, 922, 963, 1091, 1411
BssT1I	CCWWGG	1 cut(s)	531
Bst2UI	CCWGG	1 cut(s)	314
Bst4CI	ACNGT	2 cut(s)	140, 970
Bst6I	CTCTTC	1 cut(s)	1230
BstAPI	GCANNNNNTGC	1 cut(s)	514
BstC8I	GCNNGC	3 cut(s)	831, 1030, 1042
BstDEI	CTNAG	3 cut(s)	154, 892, 1337
BstDSI	CCRYGG	2 cut(s)	483, 531
BstEII	GGTNACC	1 cut(s)	1135
BstF5I	GGATG	7 cut(s)	37, 165, 333, 565, 628, 1317, 1377
BstFNI	CGCG	1 cut(s)	485
BstKTI	GATC	6 cut(s)	225, 426, 925, 966, 1094, 1414
BstMAI	GTCTC	1 cut(s)	192
BstMBI	GATC	6 cut(s)	222, 423, 922, 963, 1091, 1411
BstMWI	GCNNNNNNNGC	7 cut(s)	83, 156, 302, 308, 482, 514, 1211
BstNI	CCWGG	1 cut(s)	314
BstNSI	RCATGY	3 cut(s)	10, 833, 1225
BstPI	GGTNACC	1 cut(s)	1135
BstSCI	CCNGG	2 cut(s)	312, 321
BstSLI	GKGCMC	1 cut(s)	215
BstUI	CGCG	1 cut(s)	485
BstV1I	GCAGC	2 cut(s)	1301, 1337
BstXI	CCANNNNNNTGG	2 cut(s)	313, 532
BtgI	CCRYGG	2 cut(s)	483, 531
BtgZI	GCGATG	1 cut(s)	39
BtsCI	GGATG	7 cut(s)	37, 165, 333, 565, 628, 1317, 1377
BtsI	GCAGTG	1 cut(s)	1260
BtsIMutI	CAGTG	4 cut(s)	544, 639, 900, 1260
BveI	ACCTGC	1 cut(s)	605
Cac8I	GCNNGC	3 cut(s)	831, 1030, 1042
Cfr10I	RCCGGY	5 cut(s)	277, 365, 395, 1028, 1040
Cfr13I	GGNCC	5 cut(s)	117, 126, 280, 794, 1026
Cfr42I	CCGCGG	1 cut(s)	486
Csp6I	GTAC	2 cut(s)	493, 1048
CspAI	ACCGGT	2 cut(s)	365, 395
CspCI	CAANNNNNGTGG	2 cut(s)	159, 194
CviQI	GTAC	2 cut(s)	493, 1048
DdeI	CTNAG	3 cut(s)	154, 892, 1337
DpnI	GATC	6 cut(s)	224, 425, 924, 965, 1093, 1413
DpnII	GATC	6 cut(s)	222, 423, 922, 963, 1091, 1411
DriI	GACNNNNNGTC	1 cut(s)	10
EaeI	YGGCCR	5 cut(s)	75, 315, 348, 1038, 1042
Eam1104I	CTCTTC	1 cut(s)	1230
Eam1105I	GACNNNNNGTC	1 cut(s)	10
EarI	CTCTTC	1 cut(s)	1230
EciI	GGCGGA	1 cut(s)	1078
Eco130I	CCWWGG	1 cut(s)	531
Eco47I	GGWCC	2 cut(s)	117, 280
Eco88I	CYCGRG	1 cut(s)	47
Eco91I	GGTNACC	1 cut(s)	1135
EcoO65I	GGTNACC	1 cut(s)	1135
EcoRII	CCWGG	1 cut(s)	312
EcoT14I	CCWWGG	1 cut(s)	531
EcoT22I	ATGCAT	2 cut(s)	835, 1227
ErhI	CCWWGG	1 cut(s)	531
FaqI	GGGAC	3 cut(s)	871, 1138, 1232
FauI	CCCGC	1 cut(s)	1025
FbaI	TGATCA	1 cut(s)	1411
Fnu4HI	GCNGC	4 cut(s)	303, 351, 1290, 1351
FokI	GGATG	7 cut(s)	24, 152, 320, 572, 615, 1324, 1384
FseI	GGCCGGCC	1 cut(s)	1044
Fsp4HI	GCNGC	4 cut(s)	303, 351, 1290, 1351
FspBI	CTAG	6 cut(s)	191, 672, 1088, 1215, 1274, 1295
GluI	GCNGC	4 cut(s)	303, 351, 1290, 1351
GsaI	CCCAGC	1 cut(s)	1194
HincII	GTYRAC	1 cut(s)	480
HindII	GTYRAC	1 cut(s)	480
HinfI	GANTC	5 cut(s)	50, 234, 357, 745, 1238
HpaI	GTTAAC	1 cut(s)	480
HphI	GGTGA	5 cut(s)	220, 241, 629, 656, 1147
Hpy166II	GTNNAC	1 cut(s)	480
Hpy188I	TCNGA	2 cut(s)	922, 1004
Hpy188III	TCNNGA	1 cut(s)	47
Hpy8I	GTNNAC	1 cut(s)	480
HpyAV	CCTTC	4 cut(s)	286, 496, 635, 723
HpyCH4III	ACNGT	2 cut(s)	140, 970
HpyF10VI	GCNNNNNNNGC	7 cut(s)	83, 156, 302, 308, 482, 514, 1211
HpyF3I	CTNAG	3 cut(s)	154, 892, 1337
KroI	GCCGGC	2 cut(s)	1028, 1040
KroNI	GCCGGC	2 cut(s)	1030, 1042
Ksp22I	TGATCA	1 cut(s)	1411
KspAI	GTTAAC	1 cut(s)	480
KspI	CCGCGG	1 cut(s)	486
Kzo9I	GATC	6 cut(s)	222, 423, 922, 963, 1091, 1411
LmnI	GCTCC	2 cut(s)	254, 1004
Lsp1109I	GCAGC	2 cut(s)	1301, 1337
LweI	GCATC	3 cut(s)	342, 393, 1239
MaeI	CTAG	6 cut(s)	191, 672, 1088, 1215, 1274, 1295
MaeIII	GTNAC	2 cut(s)	1019, 1135
MalI	GATC	6 cut(s)	224, 425, 924, 965, 1093, 1413
MboI	GATC	6 cut(s)	222, 423, 922, 963, 1091, 1411
MboII	GAAGA	5 cut(s)	217, 671, 674, 829, 1247
MhlI	GDGCHC	1 cut(s)	215
MlsI	TGGCCA	1 cut(s)	317
MluNI	TGGCCA	1 cut(s)	317
MlyI	GAGTC	2 cut(s)	59, 1247
MmeI	TCCRAC	2 cut(s)	307, 537
Mox20I	TGGCCA	1 cut(s)	317
Mph1103I	ATGCAT	2 cut(s)	835, 1227
MroNI	GCCGGC	2 cut(s)	1028, 1040
MroXI	GAANNNNTTC	1 cut(s)	1151
MscI	TGGCCA	1 cut(s)	317
MseI	TTAA	6 cut(s)	185, 479, 498, 606, 725, 786
Msp20I	TGGCCA	1 cut(s)	317
MspA1I	CMGCKG	2 cut(s)	94, 485
MspR9I	CCNGG	2 cut(s)	314, 323
MvaI	CCWGG	1 cut(s)	314
MvnI	CGCG	1 cut(s)	485
MwoI	GCNNNNNNNGC	7 cut(s)	83, 156, 302, 308, 482, 514, 1211
NaeI	GCCGGC	2 cut(s)	1030, 1042
NciI	CCSGG	1 cut(s)	323
NcoI	CCATGG	1 cut(s)	531
NdeII	GATC	6 cut(s)	222, 423, 922, 963, 1091, 1411
NgoMIV	GCCGGC	2 cut(s)	1028, 1040
NlaIV	GGNNCC	6 cut(s)	160, 214, 256, 391, 1006, 1027
NmeAIII	GCCGAG	1 cut(s)	154
NmuCI	GTSAC	2 cut(s)	1019, 1135
NsiI	ATGCAT	2 cut(s)	835, 1227
NspI	RCATGY	3 cut(s)	10, 833, 1225
PaeI	GCATGC	1 cut(s)	833
PaqCI	CACCTGC	1 cut(s)	605
PcsI	WCGNNNNNNNCGW	1 cut(s)	336
PdiI	GCCGGC	2 cut(s)	1030, 1042
PdmI	GAANNNNTTC	1 cut(s)	1151
PfeI	GAWTC	3 cut(s)	234, 357, 745
PinAI	ACCGGT	2 cut(s)	365, 395
PkrI	GCNGC	4 cut(s)	304, 352, 1291, 1352
PleI	GAGTC	2 cut(s)	58, 1246
PpsI	GAGTC	2 cut(s)	58, 1246
PsiI	TTATAA	1 cut(s)	758
Psp6I	CCWGG	1 cut(s)	312
PspEI	GGTNACC	1 cut(s)	1135
PspFI	CCCAGC	1 cut(s)	1190
PspGI	CCWGG	1 cut(s)	312
PspN4I	GGNNCC	6 cut(s)	160, 214, 256, 391, 1006, 1027
PspPI	GGNCC	5 cut(s)	117, 126, 280, 794, 1026
PvuII	CAGCTG	1 cut(s)	94
RigI	GGCCGGCC	1 cut(s)	1044
RsaI	GTAC	2 cut(s)	494, 1049
RsaNI	GTAC	2 cut(s)	493, 1048
SacII	CCGCGG	1 cut(s)	486
SaqAI	TTAA	6 cut(s)	185, 479, 498, 606, 725, 786
SatI	GCNGC	4 cut(s)	303, 351, 1290, 1351
Sau3AI	GATC	6 cut(s)	222, 423, 922, 963, 1091, 1411
Sau96I	GGNCC	5 cut(s)	117, 126, 280, 794, 1026
SchI	GAGTC	2 cut(s)	59, 1247
ScrFI	CCNGG	2 cut(s)	314, 323
SduI	GDGCHC	1 cut(s)	215
SfaNI	GCATC	3 cut(s)	342, 393, 1239
Sfr303I	CCGCGG	1 cut(s)	486
SgrBI	CCGCGG	1 cut(s)	486
SinI	GGWCC	2 cut(s)	117, 280
SpeI	ACTAGT	1 cut(s)	1273
SphI	GCATGC	1 cut(s)	833
SsiI	CCGC	6 cut(s)	303, 351, 483, 485, 1032, 1063
SspI	AATATT	1 cut(s)	885
SspMI	CTAG	6 cut(s)	191, 672, 1088, 1215, 1274, 1295
StyD4I	CCNGG	2 cut(s)	312, 321
StyI	CCWWGG	1 cut(s)	531
TaaI	ACNGT	2 cut(s)	140, 970
TaqI	TCGA	3 cut(s)	962, 1283, 1390
TatI	WGTACW	2 cut(s)	492, 1047
TauI	GCSGC	2 cut(s)	305, 353
TfiI	GAWTC	3 cut(s)	234, 357, 745
Tru1I	TTAA	6 cut(s)	185, 479, 498, 606, 725, 786
Tru9I	TTAA	6 cut(s)	185, 479, 498, 606, 725, 786
TscAI	CASTG	4 cut(s)	551, 639, 900, 1267
TseFI	GTSAC	2 cut(s)	1019, 1135
TseI	GCWGC	2 cut(s)	1289, 1350
Tsp45I	GTSAC	2 cut(s)	1019, 1135
TspDTI	ATGAA	5 cut(s)	305, 583, 795, 838, 995
TspRI	CASTG	4 cut(s)	551, 639, 900, 1267
VpaK11BI	GGWCC	2 cut(s)	117, 280
XapI	RAATTY	3 cut(s)	98, 788, 911
XceI	RCATGY	3 cut(s)	10, 833, 1225
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1052, 1105, 1157
XmnI	GAANNNNTTC	1 cut(s)	1151
XspI	CTAG	6 cut(s)	191, 672, 1088, 1215, 1274, 1295
Zsp2I	ATGCAT	2 cut(s)	835, 1227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.