Rh7AG255300

Ubiquitin-2 like Rad60 SUMO-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0015785
Physical Location & Seq
Forward (+)
25840927 .. 25842159
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG255300.1

Sequence Viewer

Length: 465 bp
ATGCAGATATTCGTGAAAACCCTAACCGGCAAGACCATCACCCTCGAGGTCGAGAGCTCCGACACCATCGACAACGTCAAGGCCAAGATCCAAGACAAGGAGGGCATCCCTCCTGATCAGCAGAGGCTCATCTTCGCCGGAAAGCAGCTCGAGGACGGCCGTACCCTCGCCGACTACAACATCCAGAAGGAATCGACTCTTCATCTTGTGCTCAGGCTTCGTGGGGGTATGCAGGTTTTTGTGAAGACTTTGACAGGCAAGACAATCACTCTGGAGGTTGAGAGCTCCGATACCATTGACAATGTCAAAGCAAAGATTCAAGACAAAGAAGGAATCCCACCGGACCAGCAGAGGTTGATCTTTGCCGGAAAGCAACTGGAGGATGGCCGCACTTTGGCTGATTATAACATTCAGAAGGAGTCTACACTCCACCTTGTCCTTCGACTCCGTGGTGGTGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

154

Amino Acids

17.25

Weight (kDa)

6.14

Isoelectric Point (pI)

28.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 1.4e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 1.8e-32 Ubiquitin family
Rad60-SLD PF11976 77 - 147 2.6e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 3.4e-32 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05050 AT4G05320
fragaria_vesca FvH4_6g15632
malus_domestica MD03G1210400.v1.1
pyrus_communis pycom12g11960
rosa_chinensis RchiOBHm_Chr7g0210981 RchiOBHm_Chr7g0213511
rosa_roxburghii Rroxscaffold_3G00245820
rosa_samantha Rh7AG255300 Rh7AG275800 Rh7CG271900 Rh7CG294700
rosa_wichuraiana Rw0G023470 Rw7G021840 Rw7G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
AbsI CCTCGAGG 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 422
AciI CCGC 1 cut(s) 388
AclWI GGATC 1 cut(s) 82
AcoI YGGCCR 2 cut(s) 157, 385
AfaI GTAC 1 cut(s) 163
AfiI CCNNNNNNNGG 2 cut(s) 97, 394
AgsI TTSAA 1 cut(s) 320
AluBI AGCT 3 cut(s) 57, 148, 285
AluI AGCT 3 cut(s) 57, 148, 285
Alw21I GWGCWC 3 cut(s) 59, 213, 287
AlwI GGATC 1 cut(s) 82
Ama87I CYCGRG 2 cut(s) 44, 149
AoxI GGCC 3 cut(s) 81, 157, 385
ApeKI GCWGC 1 cut(s) 145
AspS9I GGNCC 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 31
AvaI CYCGRG 2 cut(s) 44, 149
AvaII GGWCC 1 cut(s) 343
BaeI ACNNNNGTAYC 2 cut(s) 145, 178
BanII GRGCYC 2 cut(s) 59, 287
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 3 cut(s) 59, 213, 287
BbvI GCAGC 1 cut(s) 157
BccI CCATC 3 cut(s) 44, 74, 377
BceAI ACGGC 2 cut(s) 144, 172
BclI TGATCA 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 2 cut(s) 146, 388
BlsI GCNGC 2 cut(s) 147, 389
Bme18I GGWCC 1 cut(s) 343
BmeT110I CYCGRG 2 cut(s) 44, 149
BmgT120I GGNCC 1 cut(s) 343
BmsI GCATC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 251
BpmI CTGGAG 2 cut(s) 293, 398
Bpu10I CCTNAGC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 448
BsaWI WCCGGW 1 cut(s) 340
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 394
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 448
BseGI GGATG 3 cut(s) 105, 180, 388
BseLI CCNNNNNNNGG 2 cut(s) 97, 394
BseMII CTCAG 1 cut(s) 226
BseNI ACTGG 1 cut(s) 381
BseX3I CGGCCG 1 cut(s) 157
BseXI GCAGC 1 cut(s) 157
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 3 cut(s) 83, 159, 387
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 3 cut(s) 59, 213, 287
BsiHKCI CYCGRG 2 cut(s) 44, 149
BsiSI CCGG 4 cut(s) 27, 138, 341, 366
BslI CCNNNNNNNGG 2 cut(s) 97, 394
BsnI GGCC 3 cut(s) 83, 159, 387
BsoBI CYCGRG 2 cut(s) 44, 149
Bsp1286I GDGCHC 3 cut(s) 59, 213, 287
Bsp143I GATC 3 cut(s) 87, 115, 357
BspACI CCGC 1 cut(s) 388
BspANI GGCC 3 cut(s) 83, 159, 387
BspCNI CTCAG 1 cut(s) 225
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 26
BsrI ACTGG 1 cut(s) 381
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 448
BssMI GATC 3 cut(s) 87, 115, 357
Bst6I CTCTTC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 212
BstDSI CCRYGG 1 cut(s) 448
BstF5I GGATG 3 cut(s) 105, 180, 388
BstKTI GATC 3 cut(s) 90, 118, 360
BstMBI GATC 3 cut(s) 87, 115, 357
BstMCI CGRYCG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BstZI CGGCCG 1 cut(s) 157
BsuRI GGCC 3 cut(s) 83, 159, 387
BtgI CCRYGG 1 cut(s) 448
BtsCI GGATG 3 cut(s) 105, 180, 388
BveI ACCTGC 1 cut(s) 223
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 343
Csp6I GTAC 1 cut(s) 162
CviJI RGCY 9 cut(s) 57, 83, 127, 148, 159, 217, 285, 387, 398
CviKI_1 RGCY 9 cut(s) 57, 83, 127, 148, 159, 217, 285, 387, 398
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 1 cut(s) 212
DpnI GATC 3 cut(s) 89, 117, 359
DpnII GATC 3 cut(s) 87, 115, 357
EaeI YGGCCR 2 cut(s) 157, 385
EagI CGGCCG 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Ecl136II GAGCTC 2 cut(s) 57, 285
EclXI CGGCCG 1 cut(s) 157
Eco24I GRGCYC 2 cut(s) 59, 287
Eco47I GGWCC 1 cut(s) 343
Eco52I CGGCCG 1 cut(s) 157
Eco53kI GAGCTC 2 cut(s) 57, 285
Eco88I CYCGRG 2 cut(s) 44, 149
EcoICRI GAGCTC 2 cut(s) 57, 285
EcoT38I GRGCYC 2 cut(s) 59, 287
FaiI YATR 2 cut(s) 230, 405
FbaI TGATCA 1 cut(s) 115
FblI GTMKAC 1 cut(s) 422
Fnu4HI GCNGC 2 cut(s) 146, 388
FokI GGATG 3 cut(s) 92, 167, 395
FriOI GRGCYC 2 cut(s) 59, 287
Fsp4HI GCNGC 2 cut(s) 146, 388
GluI GCNGC 2 cut(s) 146, 388
GsuI CTGGAG 2 cut(s) 293, 398
HaeIII GGCC 3 cut(s) 83, 159, 387
HapII CCGG 4 cut(s) 27, 138, 341, 366
HinfI GANTC 6 cut(s) 191, 196, 316, 333, 419, 444
HpaII CCGG 4 cut(s) 27, 138, 341, 366
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 423
Hpy188I TCNGA 3 cut(s) 61, 289, 414
Hpy188III TCNNGA 6 cut(s) 13, 52, 113, 184, 272, 320
Hpy8I GTNNAC 1 cut(s) 423
HpyAV CCTTC 4 cut(s) 181, 323, 409, 449
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 2 cut(s) 4, 232
HpyF3I CTNAG 1 cut(s) 212
HpySE526I ACGT 1 cut(s) 75
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 3 cut(s) 87, 115, 357
LmnI GCTCC 2 cut(s) 62, 290
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 1 cut(s) 114
MaeII ACGT 1 cut(s) 75
MalI GATC 3 cut(s) 89, 117, 359
MboI GATC 3 cut(s) 87, 115, 357
MboII GAAGA 3 cut(s) 124, 191, 256
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 3 cut(s) 59, 213, 287
MlyI GAGTC 3 cut(s) 190, 428, 438
MmeI TCCRAC 1 cut(s) 84
MspI CCGG 4 cut(s) 27, 138, 341, 366
NdeII GATC 3 cut(s) 87, 115, 357
PaeR7I CTCGAG 2 cut(s) 44, 149
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 3 cut(s) 191, 316, 333
PflFI GACNNNGTC 2 cut(s) 74, 302
PkrI GCNGC 2 cut(s) 147, 389
PleI GAGTC 3 cut(s) 190, 427, 438
PpsI GAGTC 3 cut(s) 190, 427, 438
PsiI TTATAA 1 cut(s) 405
Psp124BI GAGCTC 2 cut(s) 59, 287
PspPI GGNCC 1 cut(s) 343
PspXI VCTCGAGB 2 cut(s) 44, 149
PsuI RGATCY 1 cut(s) 87
PsyI GACNNNGTC 2 cut(s) 74, 302
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SacI GAGCTC 2 cut(s) 59, 287
SatI GCNGC 2 cut(s) 146, 388
Sau3AI GATC 3 cut(s) 87, 115, 357
Sau96I GGNCC 1 cut(s) 343
SchI GAGTC 3 cut(s) 190, 428, 438
SduI GDGCHC 3 cut(s) 59, 213, 287
SetI ASST 9 cut(s) 51, 59, 78, 150, 237, 279, 287, 356, 435
SfaNI GCATC 1 cut(s) 114
Sfr274I CTCGAG 2 cut(s) 44, 149
SinI GGWCC 1 cut(s) 343
SlaI CTCGAG 2 cut(s) 44, 149
SmlI CTYRAG 2 cut(s) 44, 149
SmoI CTYRAG 2 cut(s) 44, 149
SsiI CCGC 1 cut(s) 388
SstI GAGCTC 2 cut(s) 59, 287
TaiI ACGT 1 cut(s) 78
TaqI TCGA 6 cut(s) 45, 51, 69, 150, 194, 442
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 3 cut(s) 191, 316, 333
TseI GCWGC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 191
TspGWI ACGGA 1 cut(s) 437
Tth111I GACNNNGTC 2 cut(s) 74, 302
VpaK11BI GGWCC 1 cut(s) 343
XhoI CTCGAG 2 cut(s) 44, 149
XmiI GTMKAC 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.