Rw0G023470

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01086
OG0015785
Physical Location & Seq
Reverse (-)
5890 .. 6353
464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G023470.1

Sequence Viewer

Length: 414 bp
ATGCAGATCTTTGTGAAAACCCTAACCGGCAAGACCATCACCCTTGAGGGGAGAGCTCTGACACCATCGACAACGCTCATCTTCGCCGGAAAACAACTGGAGGAAGGACGGACCCTCCCCGACTATATCCAGAAGGAGGTGACTCTCCATCTCGTGCTCAGGCTTCGCGGTGGCATGCAAATTTTTGTGAAGACTTTTACTGGCAAGACCATCACTCTGGAGGTGGAGAGCTCCGATACCATTGACAATGTCAAAGCTAAGATTCAGGATCAGGAGGGTATCCTGCCGGATCAGCAGAGGCTGATCTTTGCCGGAAGGCAACTGGAGGATGGTCGTACATTGGCCGATTATAATATCCAAAAAGAGTCTACTCTGCACCTTGTACTTTGTCTCCGCGGTGGTGCTGATTTCTGA

Protein Analysis

137

Amino Acids

15.32

Weight (kDa)

6.73

Isoelectric Point (pI)

29.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 24 - 56 1e-06 Ubiquitin family
Rad60-SLD PF11976 59 - 129 2.4e-15 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 61 - 129 4.1e-30 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05050 AT4G05320
fragaria_vesca FvH4_6g15632
malus_domestica MD03G1210400.v1.1
pyrus_communis pycom12g11960
rosa_chinensis RchiOBHm_Chr7g0210981 RchiOBHm_Chr7g0213511
rosa_roxburghii Rroxscaffold_3G00245820
rosa_samantha Rh7AG255300 Rh7AG275800 Rh7CG271900 Rh7CG294700
rosa_wichuraiana Rw0G023470 Rw7G021840 Rw7G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AccI GTMKAC 1 cut(s) 368
AccII CGCG 2 cut(s) 168, 396
AciI CCGC 3 cut(s) 168, 394, 396
AclWI GGATC 2 cut(s) 276, 297
AcoI YGGCCR 1 cut(s) 342
AcsI RAATTY 1 cut(s) 180
AfaI GTAC 2 cut(s) 337, 384
AfiI CCNNNNNNNGG 2 cut(s) 48, 136
AluBI AGCT 3 cut(s) 56, 231, 257
AluI AGCT 3 cut(s) 56, 231, 257
Alw21I GWGCWC 3 cut(s) 58, 159, 233
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 2 cut(s) 276, 297
AlwNI CAGNNNCTG 1 cut(s) 301
AoxI GGCC 1 cut(s) 342
ApoI RAATTY 1 cut(s) 180
AspS9I GGNCC 1 cut(s) 111
AsuHPI GGTGA 2 cut(s) 31, 151
AvaII GGWCC 1 cut(s) 111
BanII GRGCYC 2 cut(s) 58, 233
BauI CACGAG 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 3 cut(s) 58, 159, 233
BccI CCATC 5 cut(s) 44, 73, 156, 218, 323
BciVI GTATCC 1 cut(s) 290
BcoDI GTCTC 1 cut(s) 395
BfuI GTATCC 1 cut(s) 290
BglII AGATCT 1 cut(s) 6
Bme18I GGWCC 1 cut(s) 111
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 197
BpmI CTGGAG 3 cut(s) 119, 239, 344
Bpu10I CCTNAGC 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 394
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 375, 405
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 136
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 3 cut(s) 102, 205, 327
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 2 cut(s) 48, 136
BseMII CTCAG 1 cut(s) 172
BseNI ACTGG 3 cut(s) 102, 205, 327
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 2 cut(s) 168, 396
BshFI GGCC 1 cut(s) 344
BsiHKAI GWGCWC 3 cut(s) 58, 159, 233
BsiSI CCGG 4 cut(s) 27, 87, 287, 312
BslI CCNNNNNNNGG 2 cut(s) 48, 136
BsmAI GTCTC 1 cut(s) 395
BsnI GGCC 1 cut(s) 344
Bsp1286I GDGCHC 3 cut(s) 58, 159, 233
Bsp143I GATC 4 cut(s) 6, 268, 289, 303
BspACI CCGC 3 cut(s) 168, 394, 396
BspANI GGCC 1 cut(s) 344
BspCNI CTCAG 1 cut(s) 171
BspFNI CGCG 2 cut(s) 168, 396
BspLI GGNNCC 1 cut(s) 113
BspPI GGATC 2 cut(s) 276, 297
BsrFI RCCGGY 1 cut(s) 26
BsrI ACTGG 3 cut(s) 102, 205, 327
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 4 cut(s) 6, 268, 289, 303
BssSI CACGAG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 176
BstDEI CTNAG 2 cut(s) 158, 258
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 2 cut(s) 168, 396
BstKTI GATC 4 cut(s) 9, 271, 292, 306
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 4 cut(s) 6, 268, 289, 303
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstNSI RCATGY 1 cut(s) 178
BstUI CGCG 2 cut(s) 168, 396
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 6
BstXI CCANNNNNNTGG 1 cut(s) 217
BstYI RGATCY 1 cut(s) 6
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 1 cut(s) 344
BtgI CCRYGG 1 cut(s) 394
BtsCI GGATG 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 176
CaiI CAGNNNCTG 1 cut(s) 301
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 111
Cfr42I CCGCGG 1 cut(s) 397
Csp6I GTAC 2 cut(s) 336, 383
CviAII CATG 1 cut(s) 175
CviJI RGCY 6 cut(s) 56, 163, 231, 257, 301, 344
CviKI_1 RGCY 6 cut(s) 56, 163, 231, 257, 301, 344
CviQI GTAC 2 cut(s) 336, 383
DdeI CTNAG 2 cut(s) 158, 258
DpnI GATC 4 cut(s) 8, 270, 291, 305
DpnII GATC 4 cut(s) 6, 268, 289, 303
EaeI YGGCCR 1 cut(s) 342
Ecl136II GAGCTC 2 cut(s) 56, 231
Eco24I GRGCYC 2 cut(s) 58, 233
Eco47I GGWCC 1 cut(s) 111
Eco53kI GAGCTC 2 cut(s) 56, 231
EcoICRI GAGCTC 2 cut(s) 56, 231
EcoT38I GRGCYC 2 cut(s) 58, 233
FaeI CATG 1 cut(s) 178
FaiI YATR 3 cut(s) 126, 176, 351
FatI CATG 1 cut(s) 174
FblI GTMKAC 1 cut(s) 368
FokI GGATG 1 cut(s) 341
FriOI GRGCYC 2 cut(s) 58, 233
GsuI CTGGAG 3 cut(s) 119, 239, 344
HaeIII GGCC 1 cut(s) 344
HapII CCGG 4 cut(s) 27, 87, 287, 312
Hin1II CATG 1 cut(s) 178
HinfI GANTC 3 cut(s) 142, 262, 365
HpaII CCGG 4 cut(s) 27, 87, 287, 312
HphI GGTGA 2 cut(s) 31, 151
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 3 cut(s) 60, 235, 413
Hpy188III TCNNGA 4 cut(s) 130, 218, 266, 272
Hpy8I GTNNAC 1 cut(s) 369
HpyAV CCTTC 3 cut(s) 98, 127, 309
HpyCH4V TGCA 3 cut(s) 4, 178, 376
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpyF3I CTNAG 2 cut(s) 158, 258
Hsp92II CATG 1 cut(s) 178
KspI CCGCGG 1 cut(s) 397
Kzo9I GATC 4 cut(s) 6, 268, 289, 303
LmnI GCTCC 1 cut(s) 236
MaeIII GTNAC 1 cut(s) 139
MalI GATC 4 cut(s) 8, 270, 291, 305
MboI GATC 4 cut(s) 6, 268, 289, 303
MboII GAAGA 2 cut(s) 73, 202
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 3 cut(s) 58, 159, 233
MluCI AATT 1 cut(s) 180
MlyI GAGTC 2 cut(s) 136, 374
MnlI CCTC 8 cut(s) 40, 94, 125, 130, 214, 268, 291, 319
MspA1I CMGCKG 1 cut(s) 396
MspI CCGG 4 cut(s) 27, 87, 287, 312
MvnI CGCG 2 cut(s) 168, 396
MwoI GCNNNNNNNGC 1 cut(s) 292
NdeII GATC 4 cut(s) 6, 268, 289, 303
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 139
NspI RCATGY 1 cut(s) 178
PaeI GCATGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 262
PflFI GACNNNGTC 1 cut(s) 248
PleI GAGTC 2 cut(s) 136, 373
PpsI GAGTC 2 cut(s) 136, 373
PsiI TTATAA 1 cut(s) 351
Psp124BI GAGCTC 2 cut(s) 58, 233
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 301
PsuI RGATCY 1 cut(s) 6
PsyI GACNNNGTC 1 cut(s) 248
RsaI GTAC 2 cut(s) 337, 384
RsaNI GTAC 2 cut(s) 336, 383
SacI GAGCTC 2 cut(s) 58, 233
SacII CCGCGG 1 cut(s) 397
Sau3AI GATC 4 cut(s) 6, 268, 289, 303
Sau96I GGNCC 1 cut(s) 111
SchI GAGTC 2 cut(s) 136, 374
SduI GDGCHC 3 cut(s) 58, 159, 233
SetI ASST 6 cut(s) 58, 141, 225, 233, 259, 381
Sfr303I CCGCGG 1 cut(s) 397
SgrBI CCGCGG 1 cut(s) 397
SinI GGWCC 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SphI GCATGC 1 cut(s) 178
Sse9I AATT 1 cut(s) 180
SsiI CCGC 3 cut(s) 168, 394, 396
SstI GAGCTC 2 cut(s) 58, 233
TaqI TCGA 1 cut(s) 68
TasI AATT 1 cut(s) 180
TatI WGTACW 1 cut(s) 382
TfiI GAWTC 1 cut(s) 262
TseFI GTSAC 1 cut(s) 139
Tsp45I GTSAC 1 cut(s) 139
TspGWI ACGGA 1 cut(s) 124
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 111
XapI RAATTY 1 cut(s) 180
XceI RCATGY 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.