Rh7BG232000

A Receptor for Ubiquitination Targets

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0019046
Physical Location & Seq
Forward (+)
20302900 .. 20303982
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG232000.1

Sequence Viewer

Length: 1083 bp
ATGAAAGTCATGAAACCACTTGAACCTTACATTCTCCAAATGCCAAGCGACATAATACTAGACATCCTTTGTAGACTCCCAGCCAAAACACTCCTTCAGTGCAAGTGTGTATGCAAGTCATGGCGTGGTTATCTCTCAGACCCTCAGTTCACCAAAGTCCTATTCTCACGTGCACCACCAACCACTTGCTTTTTGATCCGCGGCTTCACTCTGGATGACTTGCTTCCGTACTCAATCTGCAGACCCAGAAGAAACTTCTTGGTCGATATTGACATTAATAGCTCAAGCAGCAGCAGCTACAACTTGGCAGCAGTCAAGCTTCCCCAAGATGGAATCGCAGACTCATACGGCATGTCATATGATTTTGTAAAGATTGTAGGTTCATACAACGGCCTCGTATGTCTAAAGGACTCATCGCGCTACAGTCCCCCTTTTCACATATGCAATCCCATGACTGGTGAGTCTTTGCACTTGTCTCCTCCTCCAATGCGTACCCCCATAGATGAGAGGAAGCCGTTTGTTTCTGGTTTTGGGTTTAGTCCCATCAGCCGTGTCTATAAGGTCCTAGTTATGCACTGCAACGAATCAAAGGCCAAGGTTTTGACAGTTGGGTCTAAGATTTGGAGAAGCATTAATGCAAATAATTACGAGTTGTTTGCTCCTCATAACTCACGCATGGTTTATATGAATGGATATCTTAATTCCATTGTAGTTCATGATCGTCTTCGAAGCACTTGTCCAGTTTCTATATGTGCCTTTGATGTTGAAAGCGAGCAATTCCAGCAAATATCACCGCCTTTTATTTTAGACGATGATGCCTATTATTACAAGTACTGTAACCTTGTAATAGTGAGAGGTTGCCTTTCTTTCGTTGCCTCCAGGACTAACAAAATTAATGTCTGGGTGATGGAAAATTATGGTGTGAACGAGTCTTGGACCCATGAATACGAATTTATAGGCACATTTAAACCATACGATGTGCGAATTAAAATATGGGTACAGCTACTGAAAATAAAAGACGAAGGGCTAGTATTGCTGTTACGTGAGGACGGATTCTATGCTTCTACGTACTTGTACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_3

Protein Analysis

360

Amino Acids

41.25

Weight (kDa)

8.57

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 11 - 51 2e-10 F-box domain
F-box-like PF12937 12 - 50 5.2e-09 F-box-like
FBA_3 PF08268 119 - 317 5.2e-26 F-box associated beta propeller domain
FBA_1 PF07734 119 - 317 2.7e-12 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019046)

Species Orthologous Gene IDs
rosa_laevigata RLG00000003237
rosa_multiflora Rmu_sc0000841.1_g000026
rosa_roxburghii Rroxscaffold_3G00250670
rosa_samantha Rh7AG236500 Rh7BG232000 Rh7BG232100 Rh7CG252200 Rh7CG252300 Rh7DG242500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 460, 610
AccI GTMKAC 1 cut(s) 73
AccII CGCG 2 cut(s) 201, 418
AciI CCGC 3 cut(s) 199, 201, 794
AclWI GGATC 1 cut(s) 190
AcsI RAATTY 1 cut(s) 950
AcuI CTGAAG 1 cut(s) 80
AcvI CACGTG 1 cut(s) 170
AfaI GTAC 6 cut(s) 230, 493, 833, 999, 1070, 1076
AfiI CCNNNNNNNGG 2 cut(s) 329, 455
AgsI TTSAA 2 cut(s) 23, 767
AjnI CCWGG 1 cut(s) 878
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 4 cut(s) 282, 297, 319, 1003
AluI AGCT 4 cut(s) 282, 297, 319, 1003
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 1 cut(s) 480
Alw44I GTGCAC 1 cut(s) 171
AlwI GGATC 1 cut(s) 190
AlwNI CAGNNNCTG 1 cut(s) 1006
AoxI GGCC 2 cut(s) 391, 591
ApaLI GTGCAC 1 cut(s) 171
ApeKI GCWGC 4 cut(s) 288, 291, 294, 308
ApoI RAATTY 1 cut(s) 950
ArsI GACNNNNNNTTYG 4 cut(s) 721, 753, 882, 914
AseI ATTAAT 3 cut(s) 276, 633, 894
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 2 cut(s) 562, 936
AsuHPI GGTGA 4 cut(s) 142, 470, 783, 916
AsuII TTCGAA 1 cut(s) 727
AvaII GGWCC 2 cut(s) 562, 936
BaeGI GKGCMC 1 cut(s) 175
BbrPI CACGTG 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 716
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 4 cut(s) 300, 303, 306, 320
BccI CCATC 3 cut(s) 323, 551, 901
BceAI ACGGC 4 cut(s) 364, 406, 499, 534
BcgI CGANNNNNNTGC 2 cut(s) 638, 672
BciT130I CCWGG 1 cut(s) 880
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 3 cut(s) 59, 566, 1028
BfmI CTRYAG 2 cut(s) 238, 421
BisI GCNGC 5 cut(s) 202, 289, 292, 295, 309
BlsI GCNGC 5 cut(s) 203, 290, 293, 296, 310
BmcAI AGTACT 1 cut(s) 833
Bme1390I CCNGG 1 cut(s) 880
Bme18I GGWCC 2 cut(s) 562, 936
BmgT120I GGNCC 2 cut(s) 562, 936
BmiI GGNNCC 1 cut(s) 938
BmrFI CCNGG 1 cut(s) 880
BmsI GCATC 1 cut(s) 805
BpiI GAAGAC 1 cut(s) 716
BpmI CTGGAG 1 cut(s) 862
Bpu14I TTCGAA 1 cut(s) 727
BpuEI CTTGAG 1 cut(s) 268
BsaAI YACGTR 3 cut(s) 170, 1043, 1068
BsaJI CCNNGG 2 cut(s) 199, 594
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 455
Bse1I ACTGG 2 cut(s) 460, 740
BseBI CCWGG 1 cut(s) 880
BseDI CCNNGG 2 cut(s) 199, 594
BseGI GGATG 2 cut(s) 63, 220
BseLI CCNNNNNNNGG 2 cut(s) 329, 455
BseMII CTCAG 2 cut(s) 150, 158
BseNI ACTGG 2 cut(s) 460, 740
BseRI GAGGAG 3 cut(s) 468, 471, 651
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 4 cut(s) 300, 303, 306, 320
BseYI CCCAGC 1 cut(s) 79
Bsh1236I CGCG 2 cut(s) 201, 418
BshFI GGCC 2 cut(s) 393, 593
BsiHKAI GWGCWC 1 cut(s) 175
BslFI GGGAC 2 cut(s) 411, 525
BslI CCNNNNNNNGG 2 cut(s) 329, 455
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 2 cut(s) 411, 525
BsnI GGCC 2 cut(s) 393, 593
Bsp119I TTCGAA 1 cut(s) 727
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 2 cut(s) 195, 718
BspACI CCGC 3 cut(s) 199, 201, 794
BspANI GGCC 2 cut(s) 393, 593
BspCNI CTCAG 2 cut(s) 149, 157
BspFNI CGCG 2 cut(s) 201, 418
BspHI TCATGA 2 cut(s) 9, 715
BspLI GGNNCC 1 cut(s) 938
BspMAI CTGCAG 1 cut(s) 242
BspPI GGATC 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 727
BsrI ACTGG 2 cut(s) 460, 740
BssECI CCNNGG 2 cut(s) 199, 594
BssMI GATC 2 cut(s) 195, 718
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 880
Bst4CI ACNGT 3 cut(s) 425, 607, 836
BstBAI YACGTR 3 cut(s) 170, 1043, 1068
BstBI TTCGAA 1 cut(s) 727
BstC8I GCNNGC 1 cut(s) 773
BstDEI CTNAG 4 cut(s) 136, 144, 615, 1080
BstDSI CCRYGG 1 cut(s) 199
BstF5I GGATG 2 cut(s) 63, 220
BstFNI CGCG 2 cut(s) 201, 418
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 2 cut(s) 198, 721
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 2 cut(s) 195, 718
BstMWI GCNNNNNNNGC 4 cut(s) 288, 294, 781, 1033
BstNI CCWGG 1 cut(s) 880
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 1 cut(s) 878
BstSFI CTRYAG 2 cut(s) 238, 421
BstSLI GKGCMC 1 cut(s) 175
BstSNI TACGTA 1 cut(s) 1068
BstUI CGCG 2 cut(s) 201, 418
BstV1I GCAGC 4 cut(s) 300, 303, 306, 320
BstV2I GAAGAC 1 cut(s) 716
BsuRI GGCC 2 cut(s) 393, 593
BtgI CCRYGG 1 cut(s) 199
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 2 cut(s) 63, 220
BtsI GCAGTG 1 cut(s) 574
BtsIMutI CAGTG 2 cut(s) 104, 574
Cac8I GCNNGC 1 cut(s) 773
CaiI CAGNNNCTG 1 cut(s) 1006
CciI TCATGA 2 cut(s) 9, 715
CfoI GCGC 1 cut(s) 420
Cfr13I GGNCC 2 cut(s) 562, 936
Cfr42I CCGCGG 1 cut(s) 202
Csp6I GTAC 6 cut(s) 229, 492, 832, 998, 1069, 1075
CviAII CATG 7 cut(s) 10, 120, 352, 451, 676, 716, 941
CviQI GTAC 6 cut(s) 229, 492, 832, 998, 1069, 1075
DdeI CTNAG 4 cut(s) 136, 144, 615, 1080
DpnI GATC 2 cut(s) 197, 720
DpnII GATC 2 cut(s) 195, 718
DraI TTTAAA 1 cut(s) 967
DrdI GACNNNNNNGTC 2 cut(s) 460, 610
DseDI GACNNNNNNGTC 2 cut(s) 460, 610
Eco105I TACGTA 1 cut(s) 1068
Eco130I CCWWGG 1 cut(s) 594
Eco32I GATATC 1 cut(s) 695
Eco47I GGWCC 2 cut(s) 562, 936
Eco57I CTGAAG 1 cut(s) 80
Eco72I CACGTG 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 1 cut(s) 878
EcoRV GATATC 1 cut(s) 695
EcoT14I CCWWGG 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 7 cut(s) 13, 123, 355, 454, 679, 719, 944
FaqI GGGAC 2 cut(s) 411, 525
FatI CATG 7 cut(s) 9, 119, 351, 450, 675, 715, 940
FauNDI CATATG 2 cut(s) 358, 440
FblI GTMKAC 1 cut(s) 73
Fnu4HI GCNGC 5 cut(s) 202, 289, 292, 295, 309
FokI GGATG 2 cut(s) 50, 227
Fsp4HI GCNGC 5 cut(s) 202, 289, 292, 295, 309
FspBI CTAG 3 cut(s) 59, 566, 1028
GlaI GCGC 1 cut(s) 419
GluI GCNGC 5 cut(s) 202, 289, 292, 295, 309
GsaI CCCAGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 862
HaeIII GGCC 2 cut(s) 393, 593
HhaI GCGC 1 cut(s) 420
Hin1II CATG 7 cut(s) 13, 123, 355, 454, 679, 719, 944
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 8 cut(s) 75, 333, 341, 410, 461, 584, 929, 1053
HphI GGTGA 4 cut(s) 142, 470, 783, 916
Hpy166II GTNNAC 4 cut(s) 74, 150, 173, 925
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 3 cut(s) 10, 212, 716
Hpy8I GTNNAC 4 cut(s) 74, 150, 173, 925
HpyAV CCTTC 2 cut(s) 104, 1016
HpyCH4III ACNGT 3 cut(s) 425, 607, 836
HpyCH4IV ACGT 3 cut(s) 169, 1042, 1067
HpyCH4V TGCA 9 cut(s) 102, 114, 173, 240, 444, 469, 574, 579, 638
HpyF10VI GCNNNNNNNGC 4 cut(s) 288, 294, 781, 1033
HpyF3I CTNAG 4 cut(s) 136, 144, 615, 1080
HpySE526I ACGT 3 cut(s) 169, 1042, 1067
Hsp92II CATG 7 cut(s) 13, 123, 355, 454, 679, 719, 944
HspAI GCGC 1 cut(s) 418
KspI CCGCGG 1 cut(s) 202
Kzo9I GATC 2 cut(s) 195, 718
LmnI GCTCC 1 cut(s) 664
Lsp1109I GCAGC 4 cut(s) 300, 303, 306, 320
LweI GCATC 1 cut(s) 805
MaeI CTAG 3 cut(s) 59, 566, 1028
MaeII ACGT 3 cut(s) 169, 1042, 1067
MaeIII GTNAC 2 cut(s) 836, 1038
MalI GATC 2 cut(s) 197, 720
MboI GATC 2 cut(s) 195, 718
MboII GAAGA 2 cut(s) 261, 716
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 7 cut(s) 643, 700, 776, 891, 913, 950, 984
MlyI GAGTC 5 cut(s) 69, 335, 404, 470, 938
MnlI CCTC 9 cut(s) 153, 404, 489, 492, 501, 672, 848, 886, 1039
MseI TTAA 6 cut(s) 276, 633, 699, 894, 966, 987
MspA1I CMGCKG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 880
MvaI CCWGG 1 cut(s) 880
MvnI CGCG 2 cut(s) 201, 418
MwoI GCNNNNNNNGC 4 cut(s) 288, 294, 781, 1033
NdeI CATATG 2 cut(s) 358, 440
NdeII GATC 2 cut(s) 195, 718
NlaIII CATG 7 cut(s) 13, 123, 355, 454, 679, 719, 944
NlaIV GGNNCC 1 cut(s) 938
NspI RCATGY 1 cut(s) 355
NspV TTCGAA 1 cut(s) 727
PagI TCATGA 2 cut(s) 9, 715
PfeI GAWTC 3 cut(s) 333, 584, 1053
PfoI TCCNGGA 1 cut(s) 878
PkrI GCNGC 5 cut(s) 203, 290, 293, 296, 310
PleI GAGTC 5 cut(s) 69, 335, 404, 469, 937
PmaCI CACGTG 1 cut(s) 170
PmlI CACGTG 1 cut(s) 170
PpsI GAGTC 5 cut(s) 69, 335, 404, 469, 937
Ppu21I YACGTR 3 cut(s) 170, 1043, 1068
PpuMI RGGWCCY 1 cut(s) 562
PshBI ATTAAT 3 cut(s) 276, 633, 894
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 878
PspCI CACGTG 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 1 cut(s) 878
PspN4I GGNNCC 1 cut(s) 938
PspPI GGNCC 2 cut(s) 562, 936
PspPPI RGGWCCY 1 cut(s) 562
PstI CTGCAG 1 cut(s) 242
PstNI CAGNNNCTG 1 cut(s) 1006
RsaI GTAC 6 cut(s) 230, 493, 833, 999, 1070, 1076
RsaNI GTAC 6 cut(s) 229, 492, 832, 998, 1069, 1075
SacII CCGCGG 1 cut(s) 202
SaqAI TTAA 6 cut(s) 276, 633, 699, 894, 966, 987
SatI GCNGC 5 cut(s) 202, 289, 292, 295, 309
Sau3AI GATC 2 cut(s) 195, 718
Sau96I GGNCC 2 cut(s) 562, 936
ScaI AGTACT 1 cut(s) 833
SchI GAGTC 5 cut(s) 69, 335, 404, 470, 938
ScrFI CCNGG 1 cut(s) 880
SduI GDGCHC 1 cut(s) 175
SfaNI GCATC 1 cut(s) 805
SfcI CTRYAG 2 cut(s) 238, 421
Sfr303I CCGCGG 1 cut(s) 202
SfuI TTCGAA 1 cut(s) 727
SgrBI CCGCGG 1 cut(s) 202
SinI GGWCC 2 cut(s) 562, 936
SmlI CTYRAG 1 cut(s) 283
SmoI CTYRAG 1 cut(s) 283
SnaBI TACGTA 1 cut(s) 1068
Sse9I AATT 7 cut(s) 643, 700, 776, 891, 913, 950, 984
SsiI CCGC 3 cut(s) 199, 201, 794
SspMI CTAG 3 cut(s) 59, 566, 1028
StyD4I CCNGG 1 cut(s) 878
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 3 cut(s) 425, 607, 836
TaiI ACGT 3 cut(s) 172, 1045, 1070
TaqI TCGA 2 cut(s) 264, 727
TasI AATT 7 cut(s) 643, 700, 776, 891, 913, 950, 984
TatI WGTACW 2 cut(s) 831, 1074
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 3 cut(s) 333, 584, 1053
Tru1I TTAA 6 cut(s) 276, 633, 699, 894, 966, 987
Tru9I TTAA 6 cut(s) 276, 633, 699, 894, 966, 987
TscAI CASTG 2 cut(s) 104, 581
TseI GCWGC 4 cut(s) 288, 291, 294, 308
TspDTI ATGAA 6 cut(s) 17, 26, 372, 701, 704, 957
TspGWI ACGGA 2 cut(s) 216, 1065
TspRI CASTG 2 cut(s) 104, 581
VneI GTGCAC 1 cut(s) 171
VpaK11BI GGWCC 2 cut(s) 562, 936
VspI ATTAAT 3 cut(s) 276, 633, 894
XapI RAATTY 1 cut(s) 950
XceI RCATGY 1 cut(s) 355
XmiI GTMKAC 1 cut(s) 73
XspI CTAG 3 cut(s) 59, 566, 1028
ZrmI AGTACT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.