Rh7DG092300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0003274
Physical Location & Seq
Reverse (-)
7743678 .. 7747874
4197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG092300.1

Sequence Viewer

Length: 1680 bp
ATGGGAAGAGAAACGCCATTCACTTCAAGTTCCACAGACCCAGTAGATGCTGGAATTTTCTCTTCTTCAGCAGTAAATTCTGTGGCGACTTCATATGAGTTTCCGATGTCAGTTATGTACTCTGCATCCATGCTTCAGAGTTATTCTCAACCTGAGCTAACGTATACCACACCAGCTGCAAGTTGGAACCGTAGTAATTTGACAATGACTTTTGGTGAGGTTGGTTTGACAAAATATGATCCTTGTGCGGCTAATTCTACAGCTTCCCAGTCACCAAGGTACGATGCTTTTCTGGATCTGGGGCTTGGTTCATCAGATACCAGAATAACTTTTTCTCCCTTGAATCCCATTTTGTCAAAAAATGCTGAGGTTACAGGCAGCTTTGCAGTGAAATGCAATGATAATTCTTCTAAATACAGTCCTCCTGACATAATGGATCTTCATCAGCTGCTGTATGGTGAAGGCAAAAAGACTGACCATGATAAAAGTCCGAGTGATAAAGGAAACGAACGGAAATGTGGAAAAACTGTATCTAGTGAGGGTTCAGACCCGTTACTAACAGATAAGTCTGATCCACAAATCACTTTAGAGAAGCTTTATGATAAATCTAGTTCGGAACATCAGGATGCTGAGGCAGCTGTTTCTCTTTCAACAAAGTTAGATGGGAACGACTCTGATGTGGACTCACCTTGTTGGAGAGGAAGCCTGGCTTCTAGACAAACTCCACTTGGAGTTTCAAGGTCTTTGAGTTCACACTCTATTGAAAATGTACAAGAAGCAAGTTATAGTCTGAATCCGCTGGCGCCTCATTTTTTTCCTCGGCCTTCTAAAGCAATTGTTAATTGCTATGCAAATGAATGTGATGCGGATGATTTCTCATCTTTCAAAAACAGTGAATCTGGAGCTGTAGGGGCTGTTAGTTCATTCTCCAAGGAGAATACATCAGTTGATAAAGCAGGATCAAAGTCGTCATTATCGCTTAATGGAATGGGAACTCAAACTTCTAATGACATCCATGAACCAAAAAGAGAATATGCCCTGCTTGATAAATCAGGAAGTTACTCTGCGCTCAGCGAGGGTATATCCAAGTTATTGTCTACAGATTCAAAAACAGATGTTTCAACAGTGCTAAATATGATGCATGATCTGTCAAGTTTTCTTGTACAAAATTGTTCAAATGACGTTTTATTGAATGATCATGACTTGATCCAGCATATAATCAATAACCTATGTATGTGCTTCCAACATAGGGCTGGAGGAAAGACTCCAATGCCTGATTTTACTGTTTCAGGCACTCCATACTTTCCTAATAAGTCAACGGAGATCATCGAGGCGGGGTGCTCGAAGATGGGGTTTCAAGTAACAAAGACAGGGGCTCTTGCTGTTCCACATGAACTTGATCATCAGAATGTGGTTGGTTGTCTTGATATTACTGAGAGAATGTTGAATTCCTTTCCTTCATATAGCAACGTTACTGGGAAGAGCAAAGACATTATTCAGATGCAGGTCATGGGAAATGCTGTGAGAGACAGTTATTCAACCAAGGACGAGATAGACCCACAAGCTTTAGTATACAAAAAACTGTGGCTCGAGGCTGAAGCAACATTGCGTGCCATGAAGTATGAAAGATGTGTGATGCATGAAATTGGAAATGGGATGGCGCAATCTAAACAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

559

Amino Acids

60.69

Weight (kDa)

5.2

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1495
AccB1I GGYRCC 1 cut(s) 802
AccI GTMKAC 3 cut(s) 164, 1097, 1572
AciI CCGC 4 cut(s) 248, 797, 866, 1334
AclI AACGTT 1 cut(s) 1470
AclWI GGATC 6 cut(s) 233, 303, 444, 566, 967, 1201
AcsI RAATTY 3 cut(s) 54, 76, 1447
AcuI CTGAAG 3 cut(s) 51, 119, 1617
AcyI GRCGYC 1 cut(s) 803
AfaI GTAC 4 cut(s) 119, 281, 771, 1164
AfiI CCNNNNNNNGG 1 cut(s) 1249
AjnI CCWGG 1 cut(s) 705
AluBI AGCT 9 cut(s) 157, 176, 263, 381, 448, 595, 638, 905, 1565
AluI AGCT 9 cut(s) 157, 176, 263, 381, 448, 595, 638, 905, 1565
Alw21I GWGCWC 1 cut(s) 1343
Alw26I GTCTC 1 cut(s) 1521
AlwI GGATC 6 cut(s) 233, 303, 444, 566, 967, 1201
AlwNI CAGNNNCTG 1 cut(s) 451
Ama87I CYCGRG 1 cut(s) 1589
AoxI GGCC 1 cut(s) 821
ApeKI GCWGC 4 cut(s) 176, 378, 448, 635
ApoI RAATTY 3 cut(s) 54, 76, 1447
AspLEI GCGC 3 cut(s) 805, 1069, 1663
AsuHPI GGTGA 4 cut(s) 227, 264, 470, 678
AvaI CYCGRG 1 cut(s) 1589
BanI GGYRCC 1 cut(s) 802
BanII GRGCYC 1 cut(s) 1378
Bbv12I GWGCWC 1 cut(s) 1343
BbvCI CCTCAGC 2 cut(s) 366, 630
BbvI GCAGC 4 cut(s) 163, 390, 435, 647
BccI CCATC 3 cut(s) 656, 1342, 1651
BciT130I CCWGG 1 cut(s) 707
BclI TGATCA 2 cut(s) 1195, 1399
BcoDI GTCTC 1 cut(s) 1521
BfaI CTAG 3 cut(s) 534, 609, 714
BfmI CTRYAG 3 cut(s) 258, 906, 1098
BfoI RGCGCY 1 cut(s) 806
BfuAI ACCTGC 1 cut(s) 1495
BisI GCNGC 5 cut(s) 177, 249, 379, 449, 636
BlpI GCTNAGC 1 cut(s) 1070
BlsI GCNGC 5 cut(s) 178, 250, 380, 450, 637
Bme1390I CCNGG 1 cut(s) 707
BmeT110I CYCGRG 1 cut(s) 1589
BmiI GGNNCC 2 cut(s) 188, 804
BmrFI CCNGG 1 cut(s) 707
BmrI ACTGGG 3 cut(s) 35, 262, 1485
BmsI GCATC 8 cut(s) 37, 134, 274, 616, 853, 1128, 1491, 1626
BmuI ACTGGG 3 cut(s) 35, 262, 1485
BplI GAGNNNNNCTC 2 cut(s) 130, 162
BpmI CTGGAG 2 cut(s) 921, 1275
Bpu10I CCTNAGC 3 cut(s) 153, 366, 630
Bpu1102I GCTNAGC 1 cut(s) 1070
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 803
BsaJI CCNNGG 4 cut(s) 275, 818, 930, 1542
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 1 cut(s) 1249
Bse1I ACTGG 3 cut(s) 41, 268, 1480
Bse3DI GCAATG 2 cut(s) 403, 1604
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 4 cut(s) 275, 818, 930, 1542
BseGI GGATG 5 cut(s) 125, 631, 874, 1011, 1662
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 1 cut(s) 1249
BseMI GCAATG 2 cut(s) 403, 1604
BseMII CTCAG 5 cut(s) 144, 357, 621, 1084, 1425
BseNI ACTGG 3 cut(s) 41, 268, 1480
BseXI GCAGC 4 cut(s) 163, 390, 435, 647
BshFI GGCC 1 cut(s) 823
BshNI GGYRCC 1 cut(s) 802
BsiHKAI GWGCWC 1 cut(s) 1343
BsiHKCI CYCGRG 1 cut(s) 1589
BslI CCNNNNNNNGG 1 cut(s) 1249
BsmAI GTCTC 1 cut(s) 1521
BsnI GGCC 1 cut(s) 823
BsoBI CYCGRG 1 cut(s) 1589
Bsp1286I GDGCHC 2 cut(s) 1343, 1378
Bsp1407I TGTACA 2 cut(s) 769, 1162
Bsp1720I GCTNAGC 1 cut(s) 1070
BspACI CCGC 4 cut(s) 248, 797, 866, 1334
BspANI GGCC 1 cut(s) 823
BspCNI CTCAG 5 cut(s) 145, 358, 622, 1083, 1426
BspHI TCATGA 1 cut(s) 1198
BspLI GGNNCC 2 cut(s) 188, 804
BspMI ACCTGC 1 cut(s) 1495
BspPI GGATC 6 cut(s) 233, 303, 444, 566, 967, 1201
BspQI GCTCTTC 1 cut(s) 1475
BspT107I GGYRCC 1 cut(s) 802
BsrDI GCAATG 2 cut(s) 403, 1604
BsrGI TGTACA 2 cut(s) 769, 1162
BsrI ACTGG 3 cut(s) 41, 268, 1480
BssECI CCNNGG 4 cut(s) 275, 818, 930, 1542
BssNAI GTATAC 2 cut(s) 165, 1573
BssNI GRCGYC 1 cut(s) 803
BssT1I CCWWGG 3 cut(s) 275, 930, 1542
Bst1107I GTATAC 2 cut(s) 165, 1573
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 8 cut(s) 191, 419, 529, 893, 1126, 1285, 1532, 1584
Bst6I CTCTTC 2 cut(s) 67, 1475
BstACI GRCGYC 1 cut(s) 803
BstAUI TGTACA 2 cut(s) 769, 1162
BstC8I GCNNGC 2 cut(s) 801, 1611
BstDEI CTNAG 5 cut(s) 153, 366, 630, 1070, 1434
BstF5I GGATG 5 cut(s) 125, 631, 874, 1011, 1662
BstH2I RGCGCY 1 cut(s) 806
BstHHI GCGC 3 cut(s) 805, 1069, 1663
BstMAI GTCTC 1 cut(s) 1521
BstMWI GCNNNNNNNGC 2 cut(s) 635, 911
BstNI CCWGG 1 cut(s) 707
BstSCI CCNGG 1 cut(s) 705
BstSFI CTRYAG 3 cut(s) 258, 906, 1098
BstV1I GCAGC 4 cut(s) 163, 390, 435, 647
BstX2I RGATCY 2 cut(s) 295, 436
BstYI RGATCY 2 cut(s) 295, 436
BstZ17I GTATAC 2 cut(s) 165, 1573
BsuRI GGCC 1 cut(s) 823
BtsCI GGATG 5 cut(s) 125, 631, 874, 1011, 1662
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 3 cut(s) 393, 898, 1131
BveI ACCTGC 1 cut(s) 1495
Cac8I GCNNGC 2 cut(s) 801, 1611
CaiI CAGNNNCTG 1 cut(s) 451
CciI TCATGA 1 cut(s) 1198
CfoI GCGC 3 cut(s) 805, 1069, 1663
Csp6I GTAC 4 cut(s) 118, 280, 770, 1163
CviAII CATG 9 cut(s) 130, 479, 1016, 1142, 1199, 1391, 1510, 1615, 1640
CviQI GTAC 4 cut(s) 118, 280, 770, 1163
DdeI CTNAG 5 cut(s) 153, 366, 630, 1070, 1434
DinI GGCGCC 1 cut(s) 804
Eam1104I CTCTTC 2 cut(s) 67, 1475
EarI CTCTTC 2 cut(s) 67, 1475
Eco130I CCWWGG 3 cut(s) 275, 930, 1542
Eco24I GRGCYC 1 cut(s) 1378
Eco57I CTGAAG 3 cut(s) 51, 119, 1617
Eco88I CYCGRG 1 cut(s) 1589
EcoRI GAATTC 1 cut(s) 1447
EcoRII CCWGG 1 cut(s) 705
EcoT14I CCWWGG 3 cut(s) 275, 930, 1542
EcoT22I ATGCAT 2 cut(s) 1143, 1641
EcoT38I GRGCYC 1 cut(s) 1378
EgeI GGCGCC 1 cut(s) 804
EheI GGCGCC 1 cut(s) 804
ErhI CCWWGG 3 cut(s) 275, 930, 1542
FaeI CATG 9 cut(s) 133, 482, 1019, 1145, 1202, 1394, 1513, 1618, 1643
FalI AAGNNNNNCTT 2 cut(s) 694, 726
FatI CATG 9 cut(s) 129, 478, 1015, 1141, 1198, 1390, 1509, 1614, 1639
FauI CCCGC 1 cut(s) 1327
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 2 cut(s) 1195, 1399
FblI GTMKAC 3 cut(s) 164, 1097, 1572
Fnu4HI GCNGC 5 cut(s) 177, 249, 379, 449, 636
FokI GGATG 5 cut(s) 112, 638, 881, 998, 1669
FriOI GRGCYC 1 cut(s) 1378
Fsp4HI GCNGC 5 cut(s) 177, 249, 379, 449, 636
FspBI CTAG 3 cut(s) 534, 609, 714
GlaI GCGC 3 cut(s) 804, 1068, 1662
GluI GCNGC 5 cut(s) 177, 249, 379, 449, 636
GsuI CTGGAG 2 cut(s) 921, 1275
HaeII RGCGCY 1 cut(s) 806
HaeIII GGCC 1 cut(s) 823
HhaI GCGC 3 cut(s) 805, 1069, 1663
Hin1I GRCGYC 1 cut(s) 803
Hin1II CATG 9 cut(s) 133, 482, 1019, 1145, 1202, 1394, 1513, 1618, 1643
Hin6I GCGC 3 cut(s) 803, 1067, 1661
HinP1I GCGC 3 cut(s) 803, 1067, 1661
HincII GTYRAC 1 cut(s) 1317
HindII GTYRAC 1 cut(s) 1317
HindIII AAGCTT 2 cut(s) 593, 1563
HinfI GANTC 7 cut(s) 343, 671, 683, 793, 896, 1103, 1264
HphI GGTGA 4 cut(s) 227, 264, 470, 678
Hpy166II GTNNAC 6 cut(s) 165, 682, 752, 1098, 1317, 1573
Hpy188III TCNNGA 8 cut(s) 293, 425, 623, 714, 900, 1053, 1199, 1424
Hpy8I GTNNAC 6 cut(s) 165, 682, 752, 1098, 1317, 1573
HpyAV CCTTC 3 cut(s) 455, 834, 1467
HpyCH4III ACNGT 8 cut(s) 191, 419, 529, 893, 1126, 1285, 1532, 1584
HpyCH4IV ACGT 3 cut(s) 161, 1182, 1470
HpyCH4V TGCA 8 cut(s) 125, 179, 386, 396, 851, 1141, 1504, 1639
HpyF10VI GCNNNNNNNGC 2 cut(s) 635, 911
HpyF3I CTNAG 5 cut(s) 153, 366, 630, 1070, 1434
HpySE526I ACGT 3 cut(s) 161, 1182, 1470
Hsp92I GRCGYC 1 cut(s) 803
Hsp92II CATG 9 cut(s) 133, 482, 1019, 1145, 1202, 1394, 1513, 1618, 1643
HspAI GCGC 3 cut(s) 803, 1067, 1661
KasI GGCGCC 1 cut(s) 802
Ksp22I TGATCA 2 cut(s) 1195, 1399
LguI GCTCTTC 1 cut(s) 1475
LmnI GCTCC 1 cut(s) 902
Lsp1109I GCAGC 4 cut(s) 163, 390, 435, 647
LweI GCATC 8 cut(s) 37, 134, 274, 616, 853, 1128, 1491, 1626
MaeI CTAG 3 cut(s) 534, 609, 714
MaeII ACGT 3 cut(s) 161, 1182, 1470
MaeIII GTNAC 6 cut(s) 270, 370, 552, 1058, 1360, 1471
MboII GAAGA 7 cut(s) 18, 54, 57, 399, 431, 1357, 1492
MfeI CAATTG 1 cut(s) 834
MflI RGATCY 2 cut(s) 295, 436
MhlI GDGCHC 2 cut(s) 1343, 1378
Mly113I GGCGCC 1 cut(s) 803
MlyI GAGTC 3 cut(s) 665, 677, 1258
MmeI TCCRAC 3 cut(s) 164, 674, 1267
Mph1103I ATGCAT 2 cut(s) 1143, 1641
MseI TTAA 2 cut(s) 840, 981
MslI CAYNNNNRTG 2 cut(s) 624, 1407
MspA1I CMGCKG 4 cut(s) 176, 448, 638, 799
MspR9I CCNGG 1 cut(s) 707
MunI CAATTG 1 cut(s) 834
MvaI CCWGG 1 cut(s) 707
MwoI GCNNNNNNNGC 2 cut(s) 635, 911
NarI GGCGCC 1 cut(s) 803
NdeI CATATG 1 cut(s) 94
NlaIII CATG 9 cut(s) 133, 482, 1019, 1145, 1202, 1394, 1513, 1618, 1643
NlaIV GGNNCC 2 cut(s) 188, 804
NmeAIII GCCGAG 1 cut(s) 799
NmuCI GTSAC 1 cut(s) 270
NsiI ATGCAT 2 cut(s) 1143, 1641
PaeR7I CTCGAG 1 cut(s) 1589
PagI TCATGA 1 cut(s) 1198
PciSI GCTCTTC 1 cut(s) 1475
PfeI GAWTC 4 cut(s) 343, 793, 896, 1103
PkrI GCNGC 5 cut(s) 178, 250, 380, 450, 637
PleI GAGTC 3 cut(s) 665, 677, 1258
PluTI GGCGCC 1 cut(s) 806
PpsI GAGTC 3 cut(s) 665, 677, 1258
Psp1406I AACGTT 1 cut(s) 1470
Psp6I CCWGG 1 cut(s) 705
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 2 cut(s) 188, 804
PspXI VCTCGAGB 1 cut(s) 1589
PstNI CAGNNNCTG 1 cut(s) 451
PsuI RGATCY 2 cut(s) 295, 436
PvuII CAGCTG 3 cut(s) 176, 448, 638
RsaI GTAC 4 cut(s) 119, 281, 771, 1164
RsaNI GTAC 4 cut(s) 118, 280, 770, 1163
RseI CAYNNNNRTG 2 cut(s) 624, 1407
SapI GCTCTTC 1 cut(s) 1475
SaqAI TTAA 2 cut(s) 840, 981
SatI GCNGC 5 cut(s) 177, 249, 379, 449, 636
SchI GAGTC 3 cut(s) 665, 677, 1258
ScrFI CCNGG 1 cut(s) 707
SduI GDGCHC 2 cut(s) 1343, 1378
SfaNI GCATC 8 cut(s) 37, 134, 274, 616, 853, 1128, 1491, 1626
SfcI CTRYAG 3 cut(s) 258, 906, 1098
SfoI GGCGCC 1 cut(s) 804
Sfr274I CTCGAG 1 cut(s) 1589
SlaI CTCGAG 1 cut(s) 1589
SmiMI CAYNNNNRTG 2 cut(s) 624, 1407
SmlI CTYRAG 1 cut(s) 1589
SmoI CTYRAG 1 cut(s) 1589
SsiI CCGC 4 cut(s) 248, 797, 866, 1334
SspDI GGCGCC 1 cut(s) 802
SspMI CTAG 3 cut(s) 534, 609, 714
StyD4I CCNGG 1 cut(s) 705
StyI CCWWGG 3 cut(s) 275, 930, 1542
TaaI ACNGT 8 cut(s) 191, 419, 529, 893, 1126, 1285, 1532, 1584
TaiI ACGT 3 cut(s) 164, 1185, 1473
TaqI TCGA 3 cut(s) 1329, 1343, 1590
TatI WGTACW 3 cut(s) 117, 769, 1162
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 4 cut(s) 343, 793, 896, 1103
Tru1I TTAA 2 cut(s) 840, 981
Tru9I TTAA 2 cut(s) 840, 981
TscAI CASTG 3 cut(s) 393, 898, 1131
TseFI GTSAC 1 cut(s) 270
TseI GCWGC 4 cut(s) 176, 378, 448, 635
Tsp45I GTSAC 1 cut(s) 270
TspGWI ACGGA 2 cut(s) 526, 1334
TspRI CASTG 3 cut(s) 393, 898, 1131
XapI RAATTY 3 cut(s) 54, 76, 1447
XbaI TCTAGA 1 cut(s) 713
XcmI CCANNNNNNNNNTGG 2 cut(s) 180, 1250
XhoI CTCGAG 1 cut(s) 1589
XmiI GTMKAC 3 cut(s) 164, 1097, 1572
XspI CTAG 3 cut(s) 534, 609, 714
Zsp2I ATGCAT 2 cut(s) 1143, 1641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.