Rw0G006630

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00245
OG0015114
Physical Location & Seq
Forward (+)
252896 .. 254570
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G006630.1

Sequence Viewer

Length: 1485 bp
ATGATACTGGGACAAAGAAGGAGCCTCACAAAACCCCTGCAAAACCTCGTCCGCTTCCTCACAACCGGGTCAACCCCAGTGAACCAGGCCCACCTCCTCCGCGTCTGCACCATCCTCTACCAGCAGCAGAACTCGCCGCCCGGGTCACGCCTCCACTCCCATCTCAACTCCCTCAGCTTCAATCTCACCCACGAGTTCTTCCTCCAGGTCTGCAACAACTTCCCGCTCTCATGGCGACCCGTCTATCTCTTCTACCACTACACCCAGACCAACCCCAGTTTCACCCACACCGCCGTATCACTGAACAAAATGCTCGACGTCGTCGGAAAGTCCCGGAACATCGACCTGTTCTGGGACTTGGTTAATGAGATGGGACGGCGTCGTTTGGTGAATGACCGGACTTTTGTTATTGCGTTGAGGACTCTGGCCAAGGCCAGAGAGTTGAACAAGTGTGTGGAGTTTTTTCATGTGATGGGTCGGTGTGGGATTGACTGTAATGTGGAGACTTTGAACAAGGTGATCAAGACTCTTTGTGGGTTGAAACTTGTTGAAGAGGCCAAGTTTGTGGTGTTTAAGTTGAAGGAGAGGATTAGGCCTGATGGGGTCACTTTTAAGTCTTTGATTCAAGGGTTTTGTGATGTGGGGGATTTGGTTGAGGCTGGGAAGATTTGGAATTTGATGGTGGATCAAGGGTTCGATCCTTGCGTCGGTGCTGTTGAGAAGATGATGGAGACTCTTTTCAAGACCAATCGGGATGCTGAGGCCATGAAGCTGTTCCAGATGATGAGGTTGAAGAGGATGGAGGAGTTGGGGCTTTCCAGTTATAGGCTTGTGATTGAGTGGATGTGTAAGAAGGGGAAGGTGGAGAATGCACATGTGGTTTATGATGAAATGCGCAAGAGAGGGATCAAAGCCGATAACTTGACATGGGGGTCGCTGGTTTATGGTCTTCTGGCGAGAAGGAGAGTAAATGAAGCTTATGGGATTGTGGAAGGGATTGAGAAGCCGGATATCAGTGTGTACCATGGCTTGATGAAGGGGCTGTTGAGGTTAAAGAGGCCGGGGGAAGCGACACAAGTGTTTAGGGAGATGATCAAGAGGGGGTGTGAGCCTAACATGCACACATATGTCATGTTATTGCAAGGGCATTTGGGAAAGAGAGGGAGGAAGGGAAAGGATCCGCTTGTGAATTTCGACACCATTTTTGTTGGGGGTTTGGTTAAGGCAGGGAAGTCATTGGAAGCCACCAAGTATGTGGAGAGAGCGACTAAAAGAGGGCTTGAAGTGCCTAGATTCGATTATAACAAGTTTTTGCATTACTACTCGAACGAGGAAGGGGTGGCAATGTTTGAGGAGGTGGGGAAGAAATTAAGGGAGGCCGGAATGGTTGATTTGGCGGATATTTTCCAGAGATATGGGGAGAAAATGGCGACGAGAGATAGAAGAAGAGATAGAGCTGTCGAGCCATTAGTAGAGCGTTCATAA

Protein Analysis

494

Amino Acids

56.92

Weight (kDa)

9.6

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 274 - 317 4.5e-10 PPR repeat family
PPR_2 PF13041 336 - 383 2.6e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26500
fragaria_vesca FvH4_4g30220
malus_domestica MD09G1267500.v1.1
prunus_persica Prupe.1G285700_v2.0.a1
pyrus_communis pycom09g18040
rosa_chinensis RchiOBHm_Chr4g0438541
rosa_laevigata RLG00000006353
rosa_multiflora Rmu_sc0002327.1_g000006
rosa_roxburghii Rroxscaffold_5G00379540
rosa_rugosa Rorug04G0311200
rosa_samantha Rh4AG361700 Rh4BG373000 Rh4CG387000 Rh4DG367700
rosa_wichuraiana Rw0G006630 Rw0G016580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1302
AasI GACNNNNNNGTC 1 cut(s) 931
AatII GACGTC 1 cut(s) 321
Acc16I TGCGCA 1 cut(s) 896
AccBSI CCGCTC 1 cut(s) 226
AccII CGCG 1 cut(s) 102
AciI CCGC 7 cut(s) 52, 100, 137, 224, 291, 1181, 1397
AclWI GGATC 5 cut(s) 692, 693, 914, 1172, 1185
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 2 cut(s) 673, 1189
AcyI GRCGYC 2 cut(s) 318, 379
AfaI GTAC 1 cut(s) 1022
AfiI CCNNNNNNNGG 3 cut(s) 352, 707, 825
AflIII ACRYGT 1 cut(s) 874
AjnI CCWGG 2 cut(s) 84, 204
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AloI GAACNNNNNNTCC 2 cut(s) 677, 709
AluBI AGCT 4 cut(s) 177, 772, 977, 1457
AluI AGCT 4 cut(s) 177, 772, 977, 1457
Alw26I GTCTC 2 cut(s) 497, 725
AlwI GGATC 5 cut(s) 692, 693, 914, 1172, 1185
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 8 cut(s) 87, 426, 432, 555, 593, 762, 1058, 1377
ApeKI GCWGC 1 cut(s) 124
ApoI RAATTY 2 cut(s) 673, 1189
ArsI GACNNNNNNTTYG 4 cut(s) 386, 418, 1187, 1219
Asp700I GAANNNNTTC 1 cut(s) 773
AspLEI GCGC 1 cut(s) 897
AspS9I GGNCC 1 cut(s) 88
AsuC2I CCSGG 5 cut(s) 67, 141, 142, 334, 1062
AsuHPI GGTGA 4 cut(s) 178, 274, 400, 529
AvaI CYCGRG 1 cut(s) 140
BaeI ACNNNNGTAYC 2 cut(s) 279, 312
BalI TGGCCA 1 cut(s) 428
BamHI GGATCC 1 cut(s) 1177
BauI CACGAG 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 941
BbvCI CCTCAGC 2 cut(s) 173, 759
BbvI GCAGC 1 cut(s) 136
BccI CCATC 8 cut(s) 119, 168, 364, 466, 593, 673, 721, 793
BceAI ACGGC 2 cut(s) 278, 392
BciT130I CCWGG 2 cut(s) 86, 206
BclI TGATCA 2 cut(s) 519, 1092
BcnI CCSGG 5 cut(s) 67, 141, 142, 334, 1062
BcoDI GTCTC 2 cut(s) 497, 725
BfaI CTAG 1 cut(s) 1290
BisI GCNGC 2 cut(s) 125, 137
BlsI GCNGC 2 cut(s) 126, 138
Bme1390I CCNGG 7 cut(s) 67, 86, 141, 142, 206, 334, 1062
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 2 cut(s) 23, 1179
BmrFI CCNGG 7 cut(s) 67, 86, 141, 142, 206, 334, 1062
BmrI ACTGGG 3 cut(s) 17, 71, 270
BmsI GCATC 1 cut(s) 745
BmuI ACTGGG 3 cut(s) 17, 71, 270
BpiI GAAGAC 1 cut(s) 941
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 2 cut(s) 173, 759
BpuMI CCSGG 5 cut(s) 67, 141, 142, 334, 1062
BsaHI GRCGYC 2 cut(s) 318, 379
BsaJI CCNNGG 4 cut(s) 140, 429, 1024, 1061
BsaWI WCCGGW 1 cut(s) 396
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 707, 825
Bse1I ACTGG 4 cut(s) 12, 77, 276, 819
Bse3DI GCAATG 1 cut(s) 1350
BseBI CCWGG 2 cut(s) 86, 206
BseDI CCNNGG 4 cut(s) 140, 429, 1024, 1061
BseGI GGATG 4 cut(s) 111, 760, 804, 849
BseLI CCNNNNNNNGG 3 cut(s) 352, 707, 825
BseMI GCAATG 1 cut(s) 1350
BseMII CTCAG 2 cut(s) 187, 750
BseNI ACTGG 4 cut(s) 12, 77, 276, 819
BseRI GAGGAG 3 cut(s) 86, 818, 1367
BseXI GCAGC 1 cut(s) 136
BseYI CCCAGC 1 cut(s) 659
BsgI GTGCAG 1 cut(s) 91
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 8 cut(s) 89, 428, 434, 557, 595, 764, 1060, 1379
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 7 cut(s) 66, 141, 334, 397, 1007, 1061, 1380
BslFI GGGAC 4 cut(s) 24, 316, 368, 387
BslI CCNNNNNNNGG 3 cut(s) 352, 707, 825
BsmAI GTCTC 2 cut(s) 497, 725
BsmFI GGGAC 4 cut(s) 24, 316, 368, 387
BsmI GAATGC 1 cut(s) 874
BsnI GGCC 8 cut(s) 89, 428, 434, 557, 595, 764, 1060, 1379
BsoBI CYCGRG 1 cut(s) 140
Bsp143I GATC 6 cut(s) 519, 685, 697, 906, 1092, 1177
Bsp19I CCATGG 1 cut(s) 1024
BspACI CCGC 7 cut(s) 52, 100, 137, 224, 291, 1181, 1397
BspANI GGCC 8 cut(s) 89, 428, 434, 557, 595, 764, 1060, 1379
BspCNI CTCAG 2 cut(s) 186, 751
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 23, 1179
BspPI GGATC 5 cut(s) 692, 693, 914, 1172, 1185
BsrBI CCGCTC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 1350
BsrI ACTGG 4 cut(s) 12, 77, 276, 819
BssECI CCNNGG 4 cut(s) 140, 429, 1024, 1061
BssMI GATC 6 cut(s) 519, 685, 697, 906, 1092, 1177
BssNI GRCGYC 2 cut(s) 318, 379
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 2 cut(s) 429, 1024
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 2 cut(s) 86, 206
Bst4CI ACNGT 1 cut(s) 494
Bst6I CTCTTC 4 cut(s) 254, 546, 788, 1441
BstACI GRCGYC 2 cut(s) 318, 379
BstDEI CTNAG 2 cut(s) 173, 759
BstDSI CCRYGG 1 cut(s) 1024
BstF5I GGATG 4 cut(s) 111, 760, 804, 849
BstFNI CGCG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 897
BstKTI GATC 6 cut(s) 522, 688, 700, 909, 1095, 1180
BstMAI GTCTC 2 cut(s) 497, 725
BstMBI GATC 6 cut(s) 519, 685, 697, 906, 1092, 1177
BstMWI GCNNNNNNNGC 4 cut(s) 133, 232, 1117, 1285
BstNI CCWGG 2 cut(s) 86, 206
BstNSI RCATGY 2 cut(s) 878, 1120
BstSCI CCNGG 7 cut(s) 65, 84, 139, 140, 204, 332, 1060
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 1 cut(s) 136
BstV2I GAAGAC 1 cut(s) 941
BstX2I RGATCY 1 cut(s) 1177
BstXI CCANNNNNNTGG 3 cut(s) 565, 1255, 1415
BstYI RGATCY 1 cut(s) 1177
BsuRI GGCC 8 cut(s) 89, 428, 434, 557, 595, 764, 1060, 1379
BtgI CCRYGG 1 cut(s) 1024
BtsCI GGATG 4 cut(s) 111, 760, 804, 849
BtsIMutI CAGTG 3 cut(s) 84, 299, 1021
CfoI GCGC 1 cut(s) 897
Cfr13I GGNCC 1 cut(s) 88
Cfr9I CCCGGG 1 cut(s) 140
CseI GACGC 3 cut(s) 91, 368, 694
Csp6I GTAC 1 cut(s) 1021
CviAII CATG 8 cut(s) 231, 467, 766, 875, 927, 1025, 1117, 1132
CviQI GTAC 1 cut(s) 1021
DdeI CTNAG 2 cut(s) 173, 759
DpnI GATC 6 cut(s) 521, 687, 699, 908, 1094, 1179
DpnII GATC 6 cut(s) 519, 685, 697, 906, 1092, 1177
DrdI GACNNNNNNGTC 1 cut(s) 931
DseDI GACNNNNNNGTC 1 cut(s) 931
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 4 cut(s) 254, 546, 788, 1441
EarI CTCTTC 4 cut(s) 254, 546, 788, 1441
EciI GGCGGA 1 cut(s) 1412
Eco130I CCWWGG 2 cut(s) 429, 1024
Eco147I AGGCCT 1 cut(s) 595
Eco32I GATATC 1 cut(s) 1012
Eco88I CYCGRG 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 84, 204
EcoRV GATATC 1 cut(s) 1012
EcoT14I CCWWGG 2 cut(s) 429, 1024
ErhI CCWWGG 2 cut(s) 429, 1024
FaeI CATG 8 cut(s) 234, 470, 769, 878, 930, 1028, 1120, 1135
FaqI GGGAC 4 cut(s) 24, 316, 368, 387
FatI CATG 8 cut(s) 230, 466, 765, 874, 926, 1024, 1116, 1131
FauI CCCGC 1 cut(s) 231
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 2 cut(s) 519, 1092
Fnu4HI GCNGC 2 cut(s) 125, 137
FokI GGATG 4 cut(s) 98, 767, 811, 856
Fsp4HI GCNGC 2 cut(s) 125, 137
FspBI CTAG 1 cut(s) 1290
FspI TGCGCA 1 cut(s) 896
GlaI GCGC 1 cut(s) 896
GluI GCNGC 2 cut(s) 125, 137
GsaI CCCAGC 1 cut(s) 663
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 8 cut(s) 89, 428, 434, 557, 595, 764, 1060, 1379
HapII CCGG 7 cut(s) 66, 141, 334, 397, 1007, 1061, 1380
HgaI GACGC 3 cut(s) 91, 368, 694
HhaI GCGC 1 cut(s) 897
Hin1I GRCGYC 2 cut(s) 318, 379
Hin1II CATG 8 cut(s) 234, 470, 769, 878, 930, 1028, 1120, 1135
Hin6I GCGC 1 cut(s) 895
HinP1I GCGC 1 cut(s) 895
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HindIII AAGCTT 1 cut(s) 975
HinfI GANTC 5 cut(s) 421, 526, 622, 733, 1293
HpaII CCGG 7 cut(s) 66, 141, 334, 397, 1007, 1061, 1380
HphI GGTGA 4 cut(s) 178, 274, 400, 529
Hpy166II GTNNAC 3 cut(s) 72, 82, 1021
Hpy188I TCNGA 1 cut(s) 326
Hpy188III TCNNGA 6 cut(s) 523, 742, 752, 778, 1096, 1408
Hpy8I GTNNAC 3 cut(s) 72, 82, 1021
Hpy99I CGWCG 6 cut(s) 320, 323, 326, 384, 710, 1435
HpyAV CCTTC 9 cut(s) 12, 574, 847, 853, 954, 986, 1030, 1162, 1328
HpyCH4III ACNGT 1 cut(s) 494
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 7 cut(s) 40, 108, 213, 872, 1120, 1141, 1315
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 232, 1117, 1285
HpyF3I CTNAG 2 cut(s) 173, 759
HpySE526I ACGT 1 cut(s) 318
Hsp92I GRCGYC 2 cut(s) 318, 379
Hsp92II CATG 8 cut(s) 234, 470, 769, 878, 930, 1028, 1120, 1135
HspAI GCGC 1 cut(s) 895
Ksp22I TGATCA 2 cut(s) 519, 1092
Kzo9I GATC 6 cut(s) 519, 685, 697, 906, 1092, 1177
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 1 cut(s) 136
LweI GCATC 1 cut(s) 745
MaeI CTAG 1 cut(s) 1290
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 2 cut(s) 144, 604
MalI GATC 6 cut(s) 521, 687, 699, 908, 1094, 1179
MbiI CCGCTC 1 cut(s) 226
MboI GATC 6 cut(s) 519, 685, 697, 906, 1092, 1177
MflI RGATCY 1 cut(s) 1177
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 3 cut(s) 673, 1189, 1367
MluNI TGGCCA 1 cut(s) 428
MlyI GAGTC 3 cut(s) 415, 520, 727
MmeI TCCRAC 1 cut(s) 304
Mox20I TGGCCA 1 cut(s) 428
MroXI GAANNNNTTC 1 cut(s) 773
MscI TGGCCA 1 cut(s) 428
MseI TTAA 6 cut(s) 363, 573, 612, 1052, 1221, 1370
MslI CAYNNNNRTG 1 cut(s) 1125
Msp20I TGGCCA 1 cut(s) 428
MspI CCGG 7 cut(s) 66, 141, 334, 397, 1007, 1061, 1380
MspR9I CCNGG 7 cut(s) 67, 86, 141, 142, 206, 334, 1062
Mva1269I GAATGC 1 cut(s) 874
MvaI CCWGG 2 cut(s) 86, 206
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 4 cut(s) 133, 232, 1117, 1285
NciI CCSGG 5 cut(s) 67, 141, 142, 334, 1062
NcoI CCATGG 1 cut(s) 1024
NdeI CATATG 1 cut(s) 1126
NdeII GATC 6 cut(s) 519, 685, 697, 906, 1092, 1177
NlaIII CATG 8 cut(s) 234, 470, 769, 878, 930, 1028, 1120, 1135
NlaIV GGNNCC 2 cut(s) 23, 1179
NmuCI GTSAC 2 cut(s) 144, 604
NsbI TGCGCA 1 cut(s) 896
NspI RCATGY 2 cut(s) 878, 1120
PceI AGGCCT 1 cut(s) 595
PciI ACATGT 1 cut(s) 874
PcsI WCGNNNNNNNCGW 1 cut(s) 702
PctI GAATGC 1 cut(s) 874
PdmI GAANNNNTTC 1 cut(s) 773
PfeI GAWTC 2 cut(s) 622, 1293
PflFI GACNNNGTC 2 cut(s) 320, 378
PfoI TCCNGGA 1 cut(s) 332
PkrI GCNGC 2 cut(s) 126, 138
PleI GAGTC 3 cut(s) 415, 520, 727
PpsI GAGTC 3 cut(s) 415, 520, 727
PscI ACATGT 1 cut(s) 874
PsiI TTATAA 1 cut(s) 1302
Psp6I CCWGG 2 cut(s) 84, 204
PspFI CCCAGC 1 cut(s) 659
PspGI CCWGG 2 cut(s) 84, 204
PspN4I GGNNCC 2 cut(s) 23, 1179
PspPI GGNCC 1 cut(s) 88
PsuI RGATCY 1 cut(s) 1177
PsyI GACNNNGTC 2 cut(s) 320, 378
RsaI GTAC 1 cut(s) 1022
RsaNI GTAC 1 cut(s) 1021
RseI CAYNNNNRTG 1 cut(s) 1125
SaqAI TTAA 6 cut(s) 363, 573, 612, 1052, 1221, 1370
SatI GCNGC 2 cut(s) 125, 137
Sau3AI GATC 6 cut(s) 519, 685, 697, 906, 1092, 1177
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 3 cut(s) 415, 520, 727
ScrFI CCNGG 7 cut(s) 67, 86, 141, 142, 206, 334, 1062
SfaNI GCATC 1 cut(s) 745
SmaI CCCGGG 1 cut(s) 142
SmiMI CAYNNNNRTG 1 cut(s) 1125
Sse9I AATT 3 cut(s) 673, 1189, 1367
SseBI AGGCCT 1 cut(s) 595
SsiI CCGC 7 cut(s) 52, 100, 137, 224, 291, 1181, 1397
SspMI CTAG 1 cut(s) 1290
StuI AGGCCT 1 cut(s) 595
StyD4I CCNGG 7 cut(s) 65, 84, 139, 140, 204, 332, 1060
StyI CCWWGG 2 cut(s) 429, 1024
TaaI ACNGT 1 cut(s) 494
TaiI ACGT 1 cut(s) 321
TaqI TCGA 7 cut(s) 315, 342, 696, 1194, 1296, 1325, 1461
TasI AATT 3 cut(s) 673, 1189, 1367
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 2 cut(s) 622, 1293
Tru1I TTAA 6 cut(s) 363, 573, 612, 1052, 1221, 1370
Tru9I TTAA 6 cut(s) 363, 573, 612, 1052, 1221, 1370
TscAI CASTG 3 cut(s) 84, 306, 1021
TseFI GTSAC 2 cut(s) 144, 604
TseI GCWGC 1 cut(s) 124
Tsp45I GTSAC 2 cut(s) 144, 604
TspDTI ATGAA 6 cut(s) 455, 782, 903, 987, 1049, 1470
TspMI CCCGGG 1 cut(s) 140
TspRI CASTG 3 cut(s) 84, 306, 1021
Tth111I GACNNNGTC 2 cut(s) 320, 378
XapI RAATTY 2 cut(s) 673, 1189
XceI RCATGY 2 cut(s) 878, 1120
XcmI CCANNNNNNNNNTGG 1 cut(s) 1252
XmaI CCCGGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 773
XspI CTAG 1 cut(s) 1290
ZraI GACGTC 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.