Rw1G010270

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018459
Physical Location & Seq
Reverse (-)
22982854 .. 22984440
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010270.1

Sequence Viewer

Length: 486 bp
ATGCTTGCCACCTTGAACAACTTCAATGCCATACAGCCTCACCTGATCACCCTTTTAATAGATTCGCATGAGTTGAGTATGCATGAAGGGAATAAAGAGAGCTTGAGTGTTGCCATGGAAAAAGGGATCAACTTGCGGGAACAAATATTGAAATTGTACAGAGATTATTACCATGGTGAATTGATGAAGCTAGTTGTAATAGGTGGAGAATCTCTTGATGTACTCGAGAATTGGGTTTTGGAATTGTTTGGTAACGTCAAAAAAGGTCCCCCAGTAAATCTGGAATTCAAGGCTGAAGGTCCAATTTGGAAAGCTGGAAAACTTTACAGGCTAGAGGCTGTTAAAGATGTTCATATACTCCACTTAACATGGACATTGCCATGCCTTCGTCAAGACTATTTGAAGAAATCAGAAGATTATTTGTCTCATCTCCTTGGGCATGAGAACAGGGGAAGTTTGCATTTCTATTTCAAAGTTAAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

161

Amino Acids

18.56

Weight (kDa)

6.75

Isoelectric Point (pI)

23.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_C PF05193 46 - 153 1.8e-13 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018459)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g23070
rosa_chinensis RchiOBHm_Chr2g0132461 RchiOBHm_Chr5g0044431 RchiOBHm_Chr6g0278561
rosa_laevigata RLG00000018508
rosa_multiflora Rmu_sc0000159.1_g000019 Rmu_sc0000723.1_g000010 Rmu_sc0004935.1_g000033
rosa_rugosa Rorug05G0214000
rosa_wichuraiana Rw1G010270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 136
AclWI GGATC 1 cut(s) 134
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 2 cut(s) 158, 222
AgsI TTSAA 6 cut(s) 16, 25, 151, 289, 403, 472
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 3 cut(s) 102, 190, 314
AluI AGCT 3 cut(s) 102, 190, 314
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 1 cut(s) 134
Ama87I CYCGRG 1 cut(s) 224
ApoI RAATTY 1 cut(s) 284
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 2 cut(s) 266, 299
AsuHPI GGTGA 3 cut(s) 32, 40, 188
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 2 cut(s) 266, 299
BclI TGATCA 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 2 cut(s) 191, 332
Bme18I GGWCC 2 cut(s) 266, 299
BmeT110I CYCGRG 1 cut(s) 224
BmgT120I GGNCC 2 cut(s) 266, 299
BmiI GGNNCC 1 cut(s) 268
BmrI ACTGGG 1 cut(s) 266
BmuI ACTGGG 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 124
BsaJI CCNNGG 3 cut(s) 114, 172, 433
Bse1I ACTGG 1 cut(s) 272
Bse3DI GCAATG 1 cut(s) 374
BseDI CCNNGG 3 cut(s) 114, 172, 433
BseMI GCAATG 1 cut(s) 374
BseNI ACTGG 1 cut(s) 272
BsiHKCI CYCGRG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 252
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 252
BsoBI CYCGRG 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 2 cut(s) 45, 126
Bsp19I CCATGG 2 cut(s) 114, 172
BspACI CCGC 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 268
BspPI GGATC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 374
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 1 cut(s) 272
BssECI CCNNGG 3 cut(s) 114, 172, 433
BssMI GATC 2 cut(s) 45, 126
BssT1I CCWWGG 3 cut(s) 114, 172, 433
BstAUI TGTACA 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 6
BstDSI CCRYGG 2 cut(s) 114, 172
BstKTI GATC 2 cut(s) 48, 129
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 2 cut(s) 45, 126
BtgI CCRYGG 2 cut(s) 114, 172
Cac8I GCNNGC 1 cut(s) 6
Cfr13I GGNCC 2 cut(s) 266, 299
Csp6I GTAC 2 cut(s) 157, 221
CviAII CATG 7 cut(s) 68, 83, 115, 173, 369, 381, 440
CviJI RGCY 7 cut(s) 37, 102, 190, 293, 314, 331, 338
CviKI_1 RGCY 7 cut(s) 37, 102, 190, 293, 314, 331, 338
CviQI GTAC 2 cut(s) 157, 221
DpnI GATC 2 cut(s) 47, 128
DpnII GATC 2 cut(s) 45, 126
Eco130I CCWWGG 3 cut(s) 114, 172, 433
Eco47I GGWCC 2 cut(s) 266, 299
Eco57I CTGAAG 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 266
EcoRI GAATTC 1 cut(s) 284
EcoT14I CCWWGG 3 cut(s) 114, 172, 433
EcoT22I ATGCAT 1 cut(s) 84
ErhI CCWWGG 3 cut(s) 114, 172, 433
FaeI CATG 7 cut(s) 71, 86, 118, 176, 372, 384, 443
FaqI GGGAC 1 cut(s) 252
FatI CATG 7 cut(s) 67, 82, 114, 172, 368, 380, 439
FauI CCCGC 1 cut(s) 129
FbaI TGATCA 1 cut(s) 45
FspBI CTAG 2 cut(s) 191, 332
Hin1II CATG 7 cut(s) 71, 86, 118, 176, 372, 384, 443
HinfI GANTC 2 cut(s) 62, 209
HphI GGTGA 3 cut(s) 32, 40, 188
Hpy188I TCNGA 1 cut(s) 412
Hpy188III TCNNGA 4 cut(s) 215, 226, 281, 392
HpyAV CCTTC 3 cut(s) 80, 290, 395
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 2 cut(s) 82, 460
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 7 cut(s) 71, 86, 118, 176, 372, 384, 443
Ksp22I TGATCA 1 cut(s) 45
Kzo9I GATC 2 cut(s) 45, 126
LpnPI CCDG 6 cut(s) 56, 266, 285, 300, 313, 433
MaeI CTAG 2 cut(s) 191, 332
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 251
MalI GATC 2 cut(s) 47, 128
MboI GATC 2 cut(s) 45, 126
MboII GAAGA 2 cut(s) 415, 425
MluCI AATT 6 cut(s) 152, 179, 229, 242, 284, 303
MnlI CCTC 2 cut(s) 48, 328
Mph1103I ATGCAT 1 cut(s) 84
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 4 cut(s) 56, 342, 365, 477
MslI CAYNNNNRTG 1 cut(s) 379
NcoI CCATGG 2 cut(s) 114, 172
NdeII GATC 2 cut(s) 45, 126
NlaIII CATG 7 cut(s) 71, 86, 118, 176, 372, 384, 443
NlaIV GGNNCC 1 cut(s) 268
NsiI ATGCAT 1 cut(s) 84
PaeR7I CTCGAG 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 2 cut(s) 62, 209
PpuMI RGGWCCY 1 cut(s) 266
Psp5II RGGWCCY 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 2 cut(s) 266, 299
PspPPI RGGWCCY 1 cut(s) 266
RsaI GTAC 2 cut(s) 158, 222
RsaNI GTAC 2 cut(s) 157, 221
RseI CAYNNNNRTG 1 cut(s) 379
SaqAI TTAA 4 cut(s) 56, 342, 365, 477
Sau3AI GATC 2 cut(s) 45, 126
Sau96I GGNCC 2 cut(s) 266, 299
SetI ASST 9 cut(s) 14, 45, 104, 192, 205, 258, 268, 301, 316
Sfr274I CTCGAG 1 cut(s) 224
SinI GGWCC 2 cut(s) 266, 299
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 379
SmlI CTYRAG 2 cut(s) 103, 224
SmoI CTYRAG 2 cut(s) 103, 224
Sse9I AATT 6 cut(s) 152, 179, 229, 242, 284, 303
SsiI CCGC 1 cut(s) 136
SspI AATATT 1 cut(s) 147
SspMI CTAG 2 cut(s) 191, 332
StyI CCWWGG 3 cut(s) 114, 172, 433
TaiI ACGT 1 cut(s) 258
TaqI TCGA 1 cut(s) 225
TasI AATT 6 cut(s) 152, 179, 229, 242, 284, 303
TatI WGTACW 2 cut(s) 156, 220
TfiI GAWTC 2 cut(s) 62, 209
Tru1I TTAA 4 cut(s) 56, 342, 365, 477
Tru9I TTAA 4 cut(s) 56, 342, 365, 477
TspDTI ATGAA 3 cut(s) 99, 200, 341
VpaK11BI GGWCC 2 cut(s) 266, 299
XapI RAATTY 1 cut(s) 284
XhoI CTCGAG 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 2 cut(s) 191, 332
Zsp2I ATGCAT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.