Rw2G016880

Lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0018968
Physical Location & Seq
Forward (+)
20400601 .. 20402177
1577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G016880.1

Sequence Viewer

Length: 642 bp
ATGCCGAGAAGGTGCCACTCCGGGACCCTCAGACTAAGAAACTCACTGACTTCACAACCCATTTCTCATTCATTCTCGACAAGGGAAGGTCTTGCCACATACATTACATTTTCTCTAGCTCCTGTTGGTTTCCAAATCCCACCAAATTCAGCTGGTGGCTTCCTTAGCCTTTTCAACACCACAACCAGTGACTTCTCTTGGAACAAGATTGTTCTAGTTGAGTTCGATTCCTTTGTAAACACCGAATGGGATCCCCCATATCTGCATGCTGGTGAAGGAGGGTCTGGTTCTGTTTACTGGGGCCACCTAGTTGAATCAGATACAGCAGTTGCAGTGAAGAAAATCTCAAGCAGGTCGGGGCAGGAAAAAAAGGAGTACATAACGGAGACAACTGGATTGGCTGGAACACGAGGTTACTTGGCTCCAGAGTATCTAAGCACAGGTAAGGCTAGTAAAGAGTCAGATGTCCATAGCTTTGGGGTGGTTGCAGAGATTGCTGCTGGGAAAAAGTCATTGGTTCTTATCACAAATGATTCGGAAATGGGATTGGTGGAGTGGGTTTGGGACCGTTATGGAAAAGAAAAGCTTCTTTTGCAAATGTCTGAGATAGTCAGTGGAATGTCTAGAGGCTTTGGCATTTGA

Protein Analysis

213

Amino Acids

23.25

Weight (kDa)

6.21

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 32 - 91 4.2e-09 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018968)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0110281 RchiOBHm_Chr2g0110291
rosa_laevigata RLG00000023392 RLG00000028036
rosa_multiflora Rmu_co8372751.1_g000001
rosa_roxburghii Rroxscaffold_2G00133610 Rroxscaffold_2G00133620
rosa_samantha Rh2DG224900
rosa_wichuraiana Rw2G016880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 342
AccB1I GGYRCC 1 cut(s) 12
AclWI GGATC 2 cut(s) 245, 258
AcsI RAATTY 1 cut(s) 145
AfaI GTAC 1 cut(s) 377
AgsI TTSAA 2 cut(s) 175, 314
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 4 cut(s) 119, 152, 474, 586
AluI AGCT 4 cut(s) 119, 152, 474, 586
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 2 cut(s) 245, 258
AoxI GGCC 1 cut(s) 301
ApeKI GCWGC 1 cut(s) 497
ApoI RAATTY 1 cut(s) 145
Asp700I GAANNNNTTC 1 cut(s) 585
AspS9I GGNCC 3 cut(s) 24, 301, 565
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 2 cut(s) 24, 565
BaeI ACNNNNGTAYC 2 cut(s) 312, 345
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 12
BauI CACGAG 1 cut(s) 408
BbvI GCAGC 1 cut(s) 484
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 5 cut(s) 116, 215, 308, 450, 624
BfuAI ACCTGC 1 cut(s) 342
BisI GCNGC 1 cut(s) 498
BlsI GCNGC 1 cut(s) 499
Bme1390I CCNGG 1 cut(s) 22
Bme18I GGWCC 2 cut(s) 24, 565
BmgT120I GGNCC 3 cut(s) 24, 301, 565
BmiI GGNNCC 7 cut(s) 14, 25, 26, 252, 302, 423, 566
BmrFI CCNGG 1 cut(s) 22
BmrI ACTGGG 1 cut(s) 307
BmuI ACTGGG 1 cut(s) 307
BpmI CTGGAG 1 cut(s) 408
Bpu10I CCTNAGC 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 331
BpuMI CCSGG 1 cut(s) 22
Bse1I ACTGG 3 cut(s) 186, 302, 397
BseMII CTCAG 2 cut(s) 43, 594
BseNI ACTGG 3 cut(s) 186, 302, 397
BseXI GCAGC 1 cut(s) 484
BseYI CCCAGC 1 cut(s) 500
BshFI GGCC 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 12
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 37, 578
BsmAI GTCTC 1 cut(s) 380
BsmFI GGGAC 2 cut(s) 37, 578
BsnI GGCC 1 cut(s) 303
Bsp143I GATC 1 cut(s) 250
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 2 cut(s) 42, 595
BspLI GGNNCC 7 cut(s) 14, 25, 26, 252, 302, 423, 566
BspMI ACCTGC 1 cut(s) 342
BspPI GGATC 2 cut(s) 245, 258
BspT107I GGYRCC 1 cut(s) 12
BsrI ACTGG 3 cut(s) 186, 302, 397
BssMI GATC 1 cut(s) 250
BssSI CACGAG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 569
BstAPI GCANNNNNTGC 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 5 cut(s) 29, 35, 164, 434, 603
BstKTI GATC 1 cut(s) 253
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 1 cut(s) 250
BstMWI GCNNNNNNNGC 3 cut(s) 165, 494, 592
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 20
BstV1I GCAGC 1 cut(s) 484
BstX2I RGATCY 1 cut(s) 250
BstXI CCANNNNNNTGG 1 cut(s) 476
BstYI RGATCY 1 cut(s) 250
BsuRI GGCC 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 4 cut(s) 44, 193, 339, 619
BveI ACCTGC 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 267
Cfr13I GGNCC 3 cut(s) 24, 301, 565
Csp6I GTAC 1 cut(s) 376
CviAII CATG 1 cut(s) 266
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 5 cut(s) 29, 35, 164, 434, 603
DpnI GATC 1 cut(s) 252
DpnII GATC 1 cut(s) 250
Eco47I GGWCC 2 cut(s) 24, 565
EcoO109I RGGNCCY 1 cut(s) 24
FaeI CATG 1 cut(s) 269
FaiI YATR 6 cut(s) 100, 259, 267, 380, 471, 573
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 2 cut(s) 37, 578
FatI CATG 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 498
Fsp4HI GCNGC 1 cut(s) 498
FspBI CTAG 5 cut(s) 116, 215, 308, 450, 624
GluI GCNGC 1 cut(s) 498
GsaI CCCAGC 1 cut(s) 504
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 1 cut(s) 303
HapII CCGG 1 cut(s) 21
Hin1II CATG 1 cut(s) 269
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 4 cut(s) 227, 314, 458, 533
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 2 cut(s) 238, 295
Hpy188I TCNGA 5 cut(s) 32, 319, 463, 538, 604
Hpy188III TCNNGA 3 cut(s) 76, 425, 624
Hpy8I GTNNAC 2 cut(s) 238, 295
HpyAV CCTTC 3 cut(s) 3, 80, 269
HpyCH4III ACNGT 1 cut(s) 569
HpyCH4V TGCA 4 cut(s) 265, 332, 488, 595
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 494, 592
HpyF3I CTNAG 5 cut(s) 29, 35, 164, 434, 603
Hsp92II CATG 1 cut(s) 269
KflI GGGWCCC 1 cut(s) 24
Kzo9I GATC 1 cut(s) 250
LmnI GCTCC 2 cut(s) 124, 427
Lsp1109I GCAGC 1 cut(s) 484
MaeI CTAG 5 cut(s) 116, 215, 308, 450, 624
MaeIII GTNAC 2 cut(s) 188, 413
MalI GATC 1 cut(s) 252
MboI GATC 1 cut(s) 250
MboII GAAGA 1 cut(s) 349
MflI RGATCY 1 cut(s) 250
MluCI AATT 1 cut(s) 145
MlyI GAGTC 1 cut(s) 467
MnlI CCTC 4 cut(s) 38, 272, 404, 620
MroXI GAANNNNTTC 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 1 cut(s) 22
MwoI GCNNNNNNNGC 3 cut(s) 165, 494, 592
NciI CCSGG 1 cut(s) 22
NdeII GATC 1 cut(s) 250
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 7 cut(s) 14, 25, 26, 252, 302, 423, 566
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 1 cut(s) 269
PaeI GCATGC 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 3 cut(s) 227, 314, 533
PfoI TCCNGGA 1 cut(s) 20
PkrI GCNGC 1 cut(s) 499
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PpuMI RGGWCCY 1 cut(s) 24
Psp5II RGGWCCY 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 500
PspN4I GGNNCC 7 cut(s) 14, 25, 26, 252, 302, 423, 566
PspPI GGNCC 3 cut(s) 24, 301, 565
PspPPI RGGWCCY 1 cut(s) 24
PsuI RGATCY 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 270
SatI GCNGC 1 cut(s) 498
Sau3AI GATC 1 cut(s) 250
Sau96I GGNCC 3 cut(s) 24, 301, 565
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 1 cut(s) 22
SinI GGWCC 2 cut(s) 24, 565
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
SphI GCATGC 1 cut(s) 269
Sse9I AATT 1 cut(s) 145
SspMI CTAG 5 cut(s) 116, 215, 308, 450, 624
StyD4I CCNGG 1 cut(s) 20
TaaI ACNGT 1 cut(s) 569
TaqI TCGA 2 cut(s) 77, 225
TasI AATT 1 cut(s) 145
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 3 cut(s) 227, 314, 533
TscAI CASTG 4 cut(s) 51, 193, 339, 619
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 1 cut(s) 497
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 60
TspGWI ACGGA 1 cut(s) 398
TspRI CASTG 4 cut(s) 51, 193, 339, 619
VpaK11BI GGWCC 2 cut(s) 24, 565
XapI RAATTY 1 cut(s) 145
XbaI TCTAGA 1 cut(s) 623
XceI RCATGY 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 5 cut(s) 116, 215, 308, 450, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.