Rw2G031840

Epidermal growth factor-like domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017338
Physical Location & Seq
Reverse (-)
52229385 .. 52232615
3231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031840.1

Sequence Viewer

Length: 576 bp
ATGATAATGAGTTGTTTGTGTGGCATGGCAGATATTGTTTGTTTAGCTCCAGGGGTAAACTGTGCGCAAGGAAAATGCAAGTATTCTAATGACTCAAGTCACTCAATATATGTGCCTGTTCTCGGACGTGTGGGTTTTGACTGCGACTGTAACCCTGGTTGGAAAAAGATTCAGATCGGCCCCTTTACCTATCCATCTTGTCTCATTCCAGACTGCACTGTTAATGTTGGATGTGATTTATCTCCCCCACAACCTCCTCCTTTCCCTCCGCCACCGGTAATACCACAATGTGCACTTGTTTGGTGTGGAGACGGAACCTGCATAGCCAACGGGCCTAGTGGATACATATGCCAATGCAATGAAGGCACATTGAACATGTTGGATAATCCACAGTTAGCATGCTTGAAAACATGCGCTTTTGGAGGAGACTGCAAGAAGCTTGGCTTCCCATCCGATGGCAAATCAAATGCTCCAAGCTCTCAAACAATTGGTTCCACAAGTGTACTGAATTTCTCAAGGAATCTCTATGCAGTAACTATGATGCTGTTGACATCAATCTTTCTGACATGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.25

Weight (kDa)

5.61

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017338)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g30801 FvH4_6g30802
malus_domestica MD17G1198900.v1.1
prunus_persica Prupe.3G075500_v2.0.a1
pyrus_communis pycom06g09320
rosa_laevigata RLG00000019637
rosa_roxburghii Rroxscaffold_2G00107280
rosa_rugosa Rorug02G0339800
rosa_samantha Rh2AG388600 Rh2BG396300 Rh2CG376000 Rh2DG411400
rosa_wichuraiana Rw2G031840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 66
Acc36I ACCTGC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 455
AciI CCGC 1 cut(s) 269
AcsI RAATTY 1 cut(s) 508
AdeI CACNNNGTG 1 cut(s) 290
AfaI GTAC 1 cut(s) 504
AfiI CCNNNNNNNGG 2 cut(s) 122, 455
AflIII ACRYGT 2 cut(s) 127, 375
AgeI ACCGGT 1 cut(s) 274
AgsI TTSAA 2 cut(s) 373, 406
AjiI CACGTC 1 cut(s) 128
AjnI CCWGG 2 cut(s) 49, 154
AluBI AGCT 3 cut(s) 47, 439, 477
AluI AGCT 3 cut(s) 47, 439, 477
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 3 cut(s) 206, 303, 420
Alw44I GTGCAC 1 cut(s) 291
AoxI GGCC 2 cut(s) 178, 332
ApaLI GTGCAC 1 cut(s) 291
ApoI RAATTY 1 cut(s) 508
AsiGI ACCGGT 1 cut(s) 274
AspLEI GCGC 2 cut(s) 67, 416
AspS9I GGNCC 2 cut(s) 179, 332
BaeGI GKGCMC 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 295
BccI CCATC 3 cut(s) 202, 449, 457
BciT130I CCWGG 2 cut(s) 51, 156
BciVI GTATCC 1 cut(s) 335
BcoDI GTCTC 3 cut(s) 206, 303, 420
BfaI CTAG 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 326
BfuI GTATCC 1 cut(s) 335
Bme1390I CCNGG 2 cut(s) 51, 156
BmgBI CACGTC 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 179, 332
BmiI GGNNCC 3 cut(s) 181, 316, 493
BmrFI CCNGG 2 cut(s) 51, 156
BmsI GCATC 1 cut(s) 531
BoxI GACNNNNGTC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 79, 499
BsaBI GATNNNNATC 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 50, 154
BsaWI WCCGGW 1 cut(s) 274
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 455
Bse118I RCCGGY 1 cut(s) 274
Bse3DI GCAATG 1 cut(s) 364
Bse8I GATNNNNATC 1 cut(s) 173
BseBI CCWGG 2 cut(s) 51, 156
BseDI CCNNGG 2 cut(s) 50, 154
BseGI GGATG 2 cut(s) 236, 449
BseJI GATNNNNATC 1 cut(s) 173
BseLI CCNNNNNNNGG 2 cut(s) 122, 455
BseMI GCAATG 1 cut(s) 364
BseRI GAGGAG 2 cut(s) 246, 438
BseSI GKGCMC 1 cut(s) 295
BsgI GTGCAG 1 cut(s) 199
BshFI GGCC 2 cut(s) 180, 334
BshTI ACCGGT 1 cut(s) 274
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 275
BslI CCNNNNNNNGG 2 cut(s) 122, 455
BsmAI GTCTC 3 cut(s) 206, 303, 420
BsmBI CGTCTC 1 cut(s) 303
BsnI GGCC 2 cut(s) 180, 334
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 1 cut(s) 174
BspACI CCGC 1 cut(s) 269
BspANI GGCC 2 cut(s) 180, 334
BspLI GGNNCC 3 cut(s) 181, 316, 493
BspMI ACCTGC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 364
BsrFI RCCGGY 1 cut(s) 274
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 2 cut(s) 50, 154
BssMI GATC 1 cut(s) 174
Bst2UI CCWGG 2 cut(s) 51, 156
Bst4CI ACNGT 4 cut(s) 62, 149, 220, 393
BstC8I GCNNGC 1 cut(s) 400
BstF5I GGATG 2 cut(s) 236, 449
BstHHI GCGC 2 cut(s) 67, 416
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 3 cut(s) 206, 303, 420
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 1 cut(s) 363
BstNI CCWGG 2 cut(s) 51, 156
BstNSI RCATGY 3 cut(s) 379, 402, 414
BstPAI GACNNNNGTC 1 cut(s) 96
BstSCI CCNGG 2 cut(s) 49, 154
BstSLI GKGCMC 1 cut(s) 295
BsuI GTATCC 1 cut(s) 335
BsuRI GGCC 2 cut(s) 180, 334
BtrI CACGTC 1 cut(s) 128
BtsCI GGATG 2 cut(s) 236, 449
BtsIMutI CAGTG 1 cut(s) 216
BveI ACCTGC 1 cut(s) 326
Cac8I GCNNGC 1 cut(s) 400
CfoI GCGC 2 cut(s) 67, 416
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 179, 332
Csp6I GTAC 1 cut(s) 503
CspAI ACCGGT 1 cut(s) 274
CviAII CATG 5 cut(s) 25, 376, 399, 411, 567
CviJI RGCY 7 cut(s) 47, 180, 326, 334, 439, 444, 477
CviKI_1 RGCY 7 cut(s) 47, 180, 326, 334, 439, 444, 477
CviQI GTAC 1 cut(s) 503
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
DraIII CACNNNGTG 1 cut(s) 290
EciI GGCGGA 1 cut(s) 258
EcoRII CCWGG 2 cut(s) 49, 154
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 5 cut(s) 28, 379, 402, 414, 570
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FatI CATG 5 cut(s) 24, 375, 398, 410, 566
FauNDI CATATG 1 cut(s) 347
FokI GGATG 2 cut(s) 243, 436
FspBI CTAG 1 cut(s) 336
FspI TGCGCA 1 cut(s) 66
GlaI GCGC 2 cut(s) 66, 415
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 2 cut(s) 180, 334
HapII CCGG 1 cut(s) 275
HhaI GCGC 2 cut(s) 67, 416
Hin1II CATG 5 cut(s) 28, 379, 402, 414, 570
Hin6I GCGC 2 cut(s) 65, 414
HinP1I GCGC 2 cut(s) 65, 414
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 3 cut(s) 92, 169, 520
HpaII CCGG 1 cut(s) 275
Hpy166II GTNNAC 4 cut(s) 58, 293, 503, 549
Hpy188I TCNGA 4 cut(s) 125, 174, 454, 564
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 4 cut(s) 58, 293, 503, 549
HpyAV CCTTC 1 cut(s) 356
HpyCH4III ACNGT 4 cut(s) 62, 149, 220, 393
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 7 cut(s) 78, 216, 293, 321, 357, 432, 530
HpyF10VI GCNNNNNNNGC 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 5 cut(s) 28, 379, 402, 414, 570
HspAI GCGC 2 cut(s) 65, 414
Kzo9I GATC 1 cut(s) 174
LmnI GCTCC 2 cut(s) 52, 475
LpnPI CCDG 8 cut(s) 36, 63, 129, 141, 168, 222, 288, 331
LweI GCATC 1 cut(s) 531
MaeI CTAG 1 cut(s) 336
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 3 cut(s) 98, 149, 532
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MfeI CAATTG 1 cut(s) 486
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 2 cut(s) 486, 508
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 3 cut(s) 140, 208, 360
MnlI CCTC 4 cut(s) 264, 267, 276, 416
MseI TTAA 1 cut(s) 222
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 2 cut(s) 51, 156
MunI CAATTG 1 cut(s) 486
MvaI CCWGG 2 cut(s) 51, 156
MwoI GCNNNNNNNGC 1 cut(s) 363
NdeI CATATG 1 cut(s) 347
NdeII GATC 1 cut(s) 174
NlaIII CATG 5 cut(s) 28, 379, 402, 414, 570
NlaIV GGNNCC 3 cut(s) 181, 316, 493
NmuCI GTSAC 1 cut(s) 98
NsbI TGCGCA 1 cut(s) 66
NspI RCATGY 3 cut(s) 379, 402, 414
PaeI GCATGC 1 cut(s) 402
PciI ACATGT 1 cut(s) 375
PfeI GAWTC 2 cut(s) 169, 520
PflMI CCANNNNNTGG 1 cut(s) 455
PinAI ACCGGT 1 cut(s) 274
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PscI ACATGT 1 cut(s) 375
PshAI GACNNNNGTC 1 cut(s) 96
Psp6I CCWGG 2 cut(s) 49, 154
PspGI CCWGG 2 cut(s) 49, 154
PspN4I GGNNCC 3 cut(s) 181, 316, 493
PspPI GGNCC 2 cut(s) 179, 332
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
SaqAI TTAA 1 cut(s) 222
Sau3AI GATC 1 cut(s) 174
Sau96I GGNCC 2 cut(s) 179, 332
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 2 cut(s) 51, 156
SduI GDGCHC 1 cut(s) 295
SetI ASST 7 cut(s) 49, 130, 191, 256, 320, 441, 479
SfaNI GCATC 1 cut(s) 531
SmlI CTYRAG 2 cut(s) 94, 514
SmoI CTYRAG 2 cut(s) 94, 514
SphI GCATGC 1 cut(s) 402
Sse9I AATT 2 cut(s) 486, 508
SsiI CCGC 1 cut(s) 269
SspMI CTAG 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 49, 154
TaaI ACNGT 4 cut(s) 62, 149, 220, 393
TaiI ACGT 1 cut(s) 130
TasI AATT 2 cut(s) 486, 508
TatI WGTACW 1 cut(s) 502
TfiI GAWTC 2 cut(s) 169, 520
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 98
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 1 cut(s) 375
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 1 cut(s) 223
Van91I CCANNNNNTGG 1 cut(s) 455
VneI GTGCAC 1 cut(s) 291
XapI RAATTY 1 cut(s) 508
XceI RCATGY 3 cut(s) 379, 402, 414
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.