Rw4G019960

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013656
Physical Location & Seq
Reverse (-)
43629411 .. 43632423
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019960.1

Sequence Viewer

Length: 1557 bp
ATGGAATCGGTTGAAGAAAACCGCCAAGTGGATTTCAATGAGGATGAATATGGTGAGCCCTCATTGTCTCTAGAAGCTTCTGCTATATGTGACGTCTTTAGTGATCCTCAGGTACTCCCTAGAGTTGGAAATGAATATCAGGTTGAAATTCCCCCTCTATCAGCTGCATCTTACTGTTCTGGGCTTATAAACCATCCAACTAATGCAAAAATCACTGCTGATGATCCCTTTGAGTTTCTAATGGGGTTAACCATTCCAGTAATGAGGATATGTGAGGAAGTTAAGAGTAAAAAGCATGATTCAGAGGTAGCTTTTGATGTTGCAGTTGGTGTAGCCAACAAAAATGGTTCTTTAAAATCTAAATGCGTCAAACAGATAGACAGTGACAACTCAAAACTCAGTGCTGAGCCAATGGATATTAAGTCTGCTCATGGGATGATATCAAGTAAGTCAGTGAAATTGGGTTTGAAACAAGAAATGATGAGTGAAATGCATGAAAAGCGTAAGGGAAGACAGTACAGTCTGGTTCCTGGTTCCTCAGTTGACCACTGGAGTGACATAGAAAAAGATAGTTTTCTTCTTGCTTTATATATATTTGGGAAGAACCTCCTTCAGGTGAAGAAATTTATTGGGAGTAAACAGATGGGCGATATATTGTCATTCTACTATGGAAAATTTTATAGGTCTTGCAGCTACATAAGATGGACAGAACGTAAAAGAAAGAAGAGCAAAAGAGGTAAACTAGGAGAAAATTTATTTACAGGATTAAGGCAAGAAGTGTTGTTATCTCGTTTGCTTCCCCACTTGTCAGAAGAAGACCAAACTACTTTACTTGAGGTATCGAAGACATATCAAGAAGAAAAAATGTTACTAGAAGCATATGTATTCATTGTAAAGGCTAAAGTCGGACTGAGTGCTTTTGTACAGGCAGTAGGAATCGGTAAAGGGAAGCAAGATCTAACAGTGCTTCGGAAAAGACCGGCTCCTTTAGCTCACGGTACCCCTTTGGCAAACTGTGGTTTAGTTTCCCCAGAGTTGAAGGCTGCCATGACTCCCAGTAAAAGATCAAAGAAGGCCAAGAAGCGAGGAGCTGATGAAGTCCCCCAAGCTGAACTGAGAAGAGATAAAAGGGTCTTGTTCGACCAATTTCATGCCTATGGAGATGTCATTGGGGAGTTGCAGAAGGAGAGCATTACAAGAGCCGAGCTGGAGAAGGAACGGGCCAAAGTGAAAGATCTGGAGACACAGCTTGGTTTTCTCAAAAGAGACTTAGAGAAGACAAAGACTAAGGTGGATGAGCTGAATGCTGAGCTCATCTGTGGCTCAGGGGGTGCAGAGGAGAGGTATAAGGCTGCCGTAGAGCACTTCAAGAAGTCCAAGGAGTTTACCCAGATGAAAAATGACCTGATTAACCATGGGATCATTCGGTGTATGAATATCATGAATGCCTACTTCCCCAACCTGGAATGGAAATGCTTGGATGGTCCACCTCCTGATGATAAAGATTTGGAGGCGAAAGAGGATGATCGAACACGAAGGAAGCAGTCAGCAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

518

Amino Acids

58.37

Weight (kDa)

8.67

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7952 PF25826 184 - 314 1.4e-54 Domain of unknown function (DUF7952)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AasI GACNNNNNNGTC 1 cut(s) 519
AatII GACGTC 1 cut(s) 96
Acc65I GGTACC 1 cut(s) 998
AccB1I GGYRCC 1 cut(s) 998
AciI CCGC 1 cut(s) 22
AclWI GGATC 3 cut(s) 98, 218, 1427
AcsI RAATTY 4 cut(s) 147, 623, 674, 751
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 4 cut(s) 114, 518, 924, 1000
AfiI CCNNNNNNNGG 4 cut(s) 28, 125, 613, 1462
AgsI TTSAA 6 cut(s) 14, 37, 146, 469, 1039, 1369
AjnI CCWGG 2 cut(s) 529, 1461
AleI CACNNNNGTG 1 cut(s) 552
Alw21I GWGCWC 2 cut(s) 1314, 1365
Alw26I GTCTC 3 cut(s) 72, 1235, 1260
AlwI GGATC 3 cut(s) 98, 218, 1427
AoxI GGCC 2 cut(s) 1074, 1221
ApeKI GCWGC 4 cut(s) 164, 690, 1043, 1352
ApoI RAATTY 4 cut(s) 147, 623, 674, 751
Asp718I GGTACC 1 cut(s) 998
AspS9I GGNCC 2 cut(s) 1221, 1484
AsuHPI GGTGA 2 cut(s) 65, 628
AvaII GGWCC 1 cut(s) 1484
AxyI CCTNAGG 1 cut(s) 108
BanI GGYRCC 1 cut(s) 998
BanII GRGCYC 2 cut(s) 60, 1314
BbsI GAAGAC 4 cut(s) 517, 822, 851, 1283
Bbv12I GWGCWC 2 cut(s) 1314, 1365
BbvI GCAGC 4 cut(s) 151, 702, 1030, 1339
BccI CCATC 4 cut(s) 201, 637, 696, 1475
BceAI ACGGC 1 cut(s) 1340
BciT130I CCWGG 2 cut(s) 531, 1463
BcoDI GTCTC 3 cut(s) 72, 1235, 1260
BfaI CTAG 4 cut(s) 71, 120, 743, 872
BglII AGATCT 2 cut(s) 955, 1234
BisI GCNGC 4 cut(s) 165, 691, 1044, 1353
BlpI GCTNAGC 2 cut(s) 405, 1308
BlsI GCNGC 4 cut(s) 166, 692, 1045, 1354
Bme1390I CCNGG 2 cut(s) 531, 1463
Bme18I GGWCC 1 cut(s) 1484
BmgT120I GGNCC 2 cut(s) 1221, 1484
BmiI GGNNCC 4 cut(s) 528, 535, 984, 1000
BmrFI CCNGG 2 cut(s) 531, 1463
BmrI ACTGGG 1 cut(s) 1050
BmsI GCATC 1 cut(s) 176
BmuI ACTGGG 1 cut(s) 1050
BpiI GAAGAC 4 cut(s) 517, 822, 851, 1283
BpmI CTGGAG 3 cut(s) 571, 1229, 1259
Bpu10I CCTNAGC 1 cut(s) 1324
Bpu1102I GCTNAGC 2 cut(s) 405, 1308
BpuEI CTTGAG 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 1377, 1414
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 125, 613, 1462
Bse118I RCCGGY 1 cut(s) 979
Bse1I ACTGG 3 cut(s) 257, 554, 1056
Bse21I CCTNAGG 1 cut(s) 108
BseBI CCWGG 2 cut(s) 531, 1463
BseDI CCNNGG 2 cut(s) 1377, 1414
BseGI GGATG 6 cut(s) 49, 193, 441, 1300, 1486, 1528
BseLI CCNNNNNNNGG 4 cut(s) 28, 125, 613, 1462
BseMII CTCAG 8 cut(s) 122, 396, 412, 552, 902, 1106, 1299, 1338
BseNI ACTGG 3 cut(s) 257, 554, 1056
BseRI GAGGAG 2 cut(s) 1101, 1352
BseXI GCAGC 4 cut(s) 151, 702, 1030, 1339
BsgI GTGCAG 1 cut(s) 1353
BshFI GGCC 2 cut(s) 1076, 1223
BshNI GGYRCC 1 cut(s) 998
BsiHKAI GWGCWC 2 cut(s) 1314, 1365
BsiSI CCGG 1 cut(s) 980
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 4 cut(s) 28, 125, 613, 1462
BsmAI GTCTC 3 cut(s) 72, 1235, 1260
BsmFI GGGAC 1 cut(s) 1085
BsmI GAATGC 2 cut(s) 1309, 1450
BsnI GGCC 2 cut(s) 1076, 1223
Bsp1286I GDGCHC 3 cut(s) 60, 1314, 1365
Bsp1407I TGTACA 1 cut(s) 922
Bsp143I GATC 7 cut(s) 103, 223, 955, 1064, 1234, 1419, 1525
Bsp1720I GCTNAGC 2 cut(s) 405, 1308
Bsp19I CCATGG 1 cut(s) 1414
BspACI CCGC 1 cut(s) 22
BspANI GGCC 2 cut(s) 1076, 1223
BspCNI CTCAG 8 cut(s) 121, 397, 411, 551, 903, 1107, 1300, 1337
BspHI TCATGA 1 cut(s) 1440
BspLI GGNNCC 4 cut(s) 528, 535, 984, 1000
BspPI GGATC 3 cut(s) 98, 218, 1427
BspQI GCTCTTC 1 cut(s) 719
BspT107I GGYRCC 1 cut(s) 998
BsrFI RCCGGY 1 cut(s) 979
BsrGI TGTACA 1 cut(s) 922
BsrI ACTGG 3 cut(s) 257, 554, 1056
BssAI RCCGGY 1 cut(s) 979
BssECI CCNNGG 2 cut(s) 1377, 1414
BssMI GATC 7 cut(s) 103, 223, 955, 1064, 1234, 1419, 1525
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 1377, 1414
Bst2UI CCWGG 2 cut(s) 531, 1463
Bst4CI ACNGT 7 cut(s) 176, 383, 516, 521, 964, 998, 1016
Bst6I CTCTTC 2 cut(s) 719, 1114
BstACI GRCGYC 1 cut(s) 93
BstAUI TGTACA 1 cut(s) 922
BstDSI CCRYGG 1 cut(s) 1414
BstENI CCTNNNNNAGG 1 cut(s) 611
BstF5I GGATG 6 cut(s) 49, 193, 441, 1300, 1486, 1528
BstKTI GATC 7 cut(s) 106, 226, 958, 1067, 1237, 1422, 1528
BstMAI GTCTC 3 cut(s) 72, 1235, 1260
BstMBI GATC 7 cut(s) 103, 223, 955, 1064, 1234, 1419, 1525
BstMWI GCNNNNNNNGC 2 cut(s) 499, 989
BstNI CCWGG 2 cut(s) 531, 1463
BstSCI CCNGG 2 cut(s) 529, 1461
BstV1I GCAGC 4 cut(s) 151, 702, 1030, 1339
BstV2I GAAGAC 4 cut(s) 517, 822, 851, 1283
BstX2I RGATCY 2 cut(s) 955, 1234
BstYI RGATCY 2 cut(s) 955, 1234
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 1076, 1223
BtgI CCRYGG 1 cut(s) 1414
BtsCI GGATG 6 cut(s) 49, 193, 441, 1300, 1486, 1528
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 6 cut(s) 213, 388, 406, 459, 547, 969
CciI TCATGA 1 cut(s) 1440
Cfr10I RCCGGY 1 cut(s) 979
Cfr13I GGNCC 2 cut(s) 1221, 1484
CseI GACGC 1 cut(s) 355
Csp6I GTAC 4 cut(s) 113, 517, 923, 999
CviAII CATG 7 cut(s) 296, 431, 494, 1048, 1151, 1415, 1441
CviQI GTAC 4 cut(s) 113, 517, 923, 999
DpnI GATC 7 cut(s) 105, 225, 957, 1066, 1236, 1421, 1527
DpnII GATC 7 cut(s) 103, 223, 955, 1064, 1234, 1419, 1525
DraI TTTAAA 1 cut(s) 354
DrdI GACNNNNNNGTC 1 cut(s) 519
DseDI GACNNNNNNGTC 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 719, 1114
EarI CTCTTC 2 cut(s) 719, 1114
Ecl136II GAGCTC 1 cut(s) 1312
Eco130I CCWWGG 2 cut(s) 1377, 1414
Eco24I GRGCYC 2 cut(s) 60, 1314
Eco32I GATATC 1 cut(s) 441
Eco47I GGWCC 1 cut(s) 1484
Eco53kI GAGCTC 1 cut(s) 1312
Eco57I CTGAAG 1 cut(s) 596
Eco81I CCTNAGG 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 1312
EcoNI CCTNNNNNAGG 1 cut(s) 611
EcoRII CCWGG 2 cut(s) 529, 1461
EcoRV GATATC 1 cut(s) 441
EcoT14I CCWWGG 2 cut(s) 1377, 1414
EcoT22I ATGCAT 1 cut(s) 495
EcoT38I GRGCYC 2 cut(s) 60, 1314
ErhI CCWWGG 2 cut(s) 1377, 1414
FaeI CATG 7 cut(s) 299, 434, 497, 1051, 1154, 1418, 1444
FaqI GGGAC 1 cut(s) 1085
FatI CATG 7 cut(s) 295, 430, 493, 1047, 1150, 1414, 1440
FauNDI CATATG 1 cut(s) 880
Fnu4HI GCNGC 4 cut(s) 165, 691, 1044, 1353
FokI GGATG 6 cut(s) 56, 180, 448, 1307, 1493, 1535
FriOI GRGCYC 2 cut(s) 60, 1314
Fsp4HI GCNGC 4 cut(s) 165, 691, 1044, 1353
FspBI CTAG 4 cut(s) 71, 120, 743, 872
GluI GCNGC 4 cut(s) 165, 691, 1044, 1353
GsuI CTGGAG 3 cut(s) 571, 1229, 1259
HaeIII GGCC 2 cut(s) 1076, 1223
HapII CCGG 1 cut(s) 980
HgaI GACGC 1 cut(s) 355
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 7 cut(s) 299, 434, 497, 1051, 1154, 1418, 1444
HincII GTYRAC 2 cut(s) 249, 544
HindII GTYRAC 2 cut(s) 249, 544
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 4 cut(s) 5, 299, 936, 1051
HpaI GTTAAC 1 cut(s) 249
HpaII CCGG 1 cut(s) 980
HphI GGTGA 2 cut(s) 65, 628
Hpy166II GTNNAC 6 cut(s) 249, 544, 638, 740, 1386, 1487
Hpy188I TCNGA 4 cut(s) 304, 811, 908, 972
Hpy188III TCNNGA 6 cut(s) 71, 854, 1238, 1369, 1441, 1493
Hpy8I GTNNAC 6 cut(s) 249, 544, 638, 740, 1386, 1487
HpyAV CCTTC 6 cut(s) 620, 1033, 1066, 1177, 1207, 1530
HpyCH4III ACNGT 7 cut(s) 176, 383, 516, 521, 964, 998, 1016
HpyCH4IV ACGT 2 cut(s) 93, 712
HpyCH4V TGCA 7 cut(s) 167, 206, 323, 493, 690, 1180, 1334
HpyF10VI GCNNNNNNNGC 2 cut(s) 499, 989
HpySE526I ACGT 2 cut(s) 93, 712
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 7 cut(s) 299, 434, 497, 1051, 1154, 1418, 1444
KpnI GGTACC 1 cut(s) 1002
KspAI GTTAAC 1 cut(s) 249
Kzo9I GATC 7 cut(s) 103, 223, 955, 1064, 1234, 1419, 1525
LguI GCTCTTC 1 cut(s) 719
LmnI GCTCC 2 cut(s) 988, 1088
Lsp1109I GCAGC 4 cut(s) 151, 702, 1030, 1339
LweI GCATC 1 cut(s) 176
MaeI CTAG 4 cut(s) 71, 120, 743, 872
MaeII ACGT 2 cut(s) 93, 712
MaeIII GTNAC 4 cut(s) 89, 383, 554, 867
MalI GATC 7 cut(s) 105, 225, 957, 1066, 1236, 1421, 1527
MboI GATC 7 cut(s) 103, 223, 955, 1064, 1234, 1419, 1525
MflI RGATCY 2 cut(s) 955, 1234
MhlI GDGCHC 3 cut(s) 60, 1314, 1365
MluCI AATT 6 cut(s) 147, 458, 623, 674, 751, 1145
MlyI GAGTC 1 cut(s) 1045
MmeI TCCRAC 3 cut(s) 106, 221, 886
Mph1103I ATGCAT 1 cut(s) 495
MseI TTAA 6 cut(s) 248, 282, 353, 420, 767, 1410
MslI CAYNNNNRTG 2 cut(s) 552, 1155
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 1 cut(s) 980
MspR9I CCNGG 2 cut(s) 531, 1463
Mva1269I GAATGC 2 cut(s) 1309, 1450
MvaI CCWGG 2 cut(s) 531, 1463
MwoI GCNNNNNNNGC 2 cut(s) 499, 989
NcoI CCATGG 1 cut(s) 1414
NdeI CATATG 1 cut(s) 880
NdeII GATC 7 cut(s) 103, 223, 955, 1064, 1234, 1419, 1525
NlaIII CATG 7 cut(s) 299, 434, 497, 1051, 1154, 1418, 1444
NlaIV GGNNCC 4 cut(s) 528, 535, 984, 1000
NmeAIII GCCGAG 1 cut(s) 1228
NmuCI GTSAC 3 cut(s) 89, 383, 554
NsiI ATGCAT 1 cut(s) 495
OliI CACNNNNGTG 1 cut(s) 552
PagI TCATGA 1 cut(s) 1440
PciSI GCTCTTC 1 cut(s) 719
PctI GAATGC 2 cut(s) 1309, 1450
PfeI GAWTC 3 cut(s) 5, 299, 936
PkrI GCNGC 4 cut(s) 166, 692, 1045, 1354
PleI GAGTC 1 cut(s) 1045
PpsI GAGTC 1 cut(s) 1045
PsiI TTATAA 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 1314
Psp6I CCWGG 2 cut(s) 529, 1461
PspGI CCWGG 2 cut(s) 529, 1461
PspN4I GGNNCC 4 cut(s) 528, 535, 984, 1000
PspPI GGNCC 2 cut(s) 1221, 1484
PsuI RGATCY 2 cut(s) 955, 1234
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 4 cut(s) 114, 518, 924, 1000
RsaNI GTAC 4 cut(s) 113, 517, 923, 999
RseI CAYNNNNRTG 2 cut(s) 552, 1155
SacI GAGCTC 1 cut(s) 1314
SapI GCTCTTC 1 cut(s) 719
SaqAI TTAA 6 cut(s) 248, 282, 353, 420, 767, 1410
SatI GCNGC 4 cut(s) 165, 691, 1044, 1353
Sau3AI GATC 7 cut(s) 103, 223, 955, 1064, 1234, 1419, 1525
Sau96I GGNCC 2 cut(s) 1221, 1484
SchI GAGTC 1 cut(s) 1045
ScrFI CCNGG 2 cut(s) 531, 1463
SduI GDGCHC 3 cut(s) 60, 1314, 1365
SfaNI GCATC 1 cut(s) 176
SinI GGWCC 1 cut(s) 1484
SmiMI CAYNNNNRTG 2 cut(s) 552, 1155
SmlI CTYRAG 1 cut(s) 833
SmoI CTYRAG 1 cut(s) 833
Sse9I AATT 6 cut(s) 147, 458, 623, 674, 751, 1145
SsiI CCGC 1 cut(s) 22
SspMI CTAG 4 cut(s) 71, 120, 743, 872
SstI GAGCTC 1 cut(s) 1314
StyD4I CCNGG 2 cut(s) 529, 1461
StyI CCWWGG 2 cut(s) 1377, 1414
TaaI ACNGT 7 cut(s) 176, 383, 516, 521, 964, 998, 1016
TaiI ACGT 2 cut(s) 96, 715
TaqI TCGA 3 cut(s) 842, 1140, 1528
TasI AATT 6 cut(s) 147, 458, 623, 674, 751, 1145
TatI WGTACW 2 cut(s) 516, 922
TfiI GAWTC 3 cut(s) 5, 299, 936
Tru1I TTAA 6 cut(s) 248, 282, 353, 420, 767, 1410
Tru9I TTAA 6 cut(s) 248, 282, 353, 420, 767, 1410
TscAI CASTG 6 cut(s) 220, 388, 406, 459, 554, 969
TseFI GTSAC 3 cut(s) 89, 383, 554
TseI GCWGC 4 cut(s) 164, 690, 1043, 1352
Tsp45I GTSAC 3 cut(s) 89, 383, 554
TspDTI ATGAA 9 cut(s) 60, 147, 510, 877, 1110, 1139, 1409, 1448, 1457
TspRI CASTG 6 cut(s) 220, 388, 406, 459, 554, 969
VpaK11BI GGWCC 1 cut(s) 1484
XagI CCTNNNNNAGG 1 cut(s) 611
XapI RAATTY 4 cut(s) 147, 623, 674, 751
XbaI TCTAGA 1 cut(s) 70
XspI CTAG 4 cut(s) 71, 120, 743, 872
ZraI GACGTC 1 cut(s) 94
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.