Rw4G020560

Peroxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017649
Physical Location & Seq
Reverse (-)
44591062 .. 44592428
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020560.1

Sequence Viewer

Length: 990 bp
ATGGCAAACTTGTTCATCATCACCTGCTTCAGTTTGCTGATCTTGGTGGTTTTGGTCTCGTCACCGGTCGACGCCTCTGTGCCTTTGGAGGTTGGTTTCTACCGGAAATCATGCCCCTCGGCCGAGACGATTGTGAGAGATACATTGAAGAAGGCAGCTGCAACCGATCCTGCCATAGCTGCTGCCCTCATCAGACTCCATTTCCATGATTGCTTTGTGAGGGGTTGCGATGCATCAATTTTGCTGGATTCTAAACCGGGGAAGCCAGCTGCAGAAAAAGAAAGCATGGGAAACAAAGGCGTCCAAGGCTTTGAGGTCATAGACGAAGCCAAGGCCAGGATAGAAGCTCAGTGCCCCAACACTGTCTCCTGTGCAGACATAATCACCTTCGCTGCCCGAGACAGTGTTTTCACCGCCGGGGGAACTTACTATTCAGCCCCCGGAGGCCGCCGTGACGGAACGGTCTCGTTGGCTTCGGAAGTGACCAAAAACCTCCCTGATGCATTCTTCAACGCAACACAACTGAAGACCAACTTTGCAAGAAAAGGGTTGTCTCTAGAGGACATGGTGACACTCTCCGGAGCTCACTCCATTGGGGACTCACACTGCTCTTCCTTCTCAAAACGACTATACTCCTTTAACAAAACGCATGCACAAGACCCTGAGATAAATAAGGCTTATGGGGAGTACTTGAAATCCAAATGCCCTAATGTGAAAAATGGTGTTGACCCGGTTGTGGCTTTTGATCCTTTGACCCCAACTTTGCTTGATAACAACTACTATAAGAATTTGGTGAGTCACAAGGGGCTGTTGGCTTCGGACCAGGAGCTTTGGAGCAGTGACTTAACTAGGAAGATGGTAAAGTACCGCAGGAACCATCCGAGGAAATGGGGTTTGGAGTTTGCTGCTGCGATGGTGAAGATGGGTTCCATTGATGTGTTGGTTGGGAAACAAGGAGAGATTAGGAAGAACTGCAGGGCCGTGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

329

Amino Acids

35.83

Weight (kDa)

8.85

Isoelectric Point (pI)

33.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 45 - 289 1.6e-85 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017649)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g18000
prunus_persica Prupe.1G137100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0422201
rosa_laevigata RLG00000007628
rosa_multiflora Rmu_co8258485.1_g000001
rosa_roxburghii Rroxscaffold_5G00364320
rosa_rugosa Rorug04G0178500
rosa_samantha Rh4AG237600 Rh4BG240800 Rh4CG252700 Rh4DG236400
rosa_wichuraiana Rw4G020560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 32
AasI GACNNNNNNGTC 1 cut(s) 461
Acc36I ACCTGC 1 cut(s) 32
AccI GTMKAC 1 cut(s) 69
AccIII TCCGGA 1 cut(s) 578
AciI CCGC 3 cut(s) 414, 448, 868
AclWI GGATC 2 cut(s) 161, 740
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 1 cut(s) 787
AcuI CTGAAG 2 cut(s) 13, 545
AcyI GRCGYC 2 cut(s) 72, 300
AfaI GTAC 2 cut(s) 689, 866
AfiI CCNNNNNNNGG 2 cut(s) 336, 736
AgeI ACCGGT 1 cut(s) 64
AgsI TTSAA 3 cut(s) 148, 511, 694
AjnI CCWGG 2 cut(s) 335, 822
AjuI GAANNNNNNNTTGG 2 cut(s) 878, 910
AluBI AGCT 6 cut(s) 158, 179, 269, 347, 584, 829
AluI AGCT 6 cut(s) 158, 179, 269, 347, 584, 829
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 6 cut(s) 61, 119, 370, 393, 469, 558
AlwI GGATC 2 cut(s) 161, 740
Ama87I CYCGRG 1 cut(s) 396
Aor13HI TCCGGA 1 cut(s) 578
AoxI GGCC 4 cut(s) 120, 333, 445, 978
ApeKI GCWGC 8 cut(s) 155, 158, 179, 182, 269, 392, 905, 908
ApoI RAATTY 1 cut(s) 787
ArsI GACNNNNNNTTYG 2 cut(s) 745, 777
AsiGI ACCGGT 1 cut(s) 64
AspS9I GGNCC 2 cut(s) 820, 978
AsuC2I CCSGG 4 cut(s) 258, 418, 441, 731
AsuHPI GGTGA 7 cut(s) 13, 54, 376, 403, 580, 805, 928
AvaI CYCGRG 1 cut(s) 396
AvaII GGWCC 1 cut(s) 820
BaeGI GKGCMC 1 cut(s) 356
BanII GRGCYC 1 cut(s) 586
BbsI GAAGAC 1 cut(s) 533
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 8 cut(s) 145, 166, 167, 169, 256, 379, 892, 895
BccI CCATC 4 cut(s) 850, 885, 907, 916
BceAI ACGGC 2 cut(s) 435, 965
BciT130I CCWGG 2 cut(s) 337, 824
BcnI CCSGG 4 cut(s) 258, 418, 441, 731
BcoDI GTCTC 6 cut(s) 61, 119, 370, 393, 469, 558
BfaI CTAG 2 cut(s) 557, 849
BfmI CTRYAG 2 cut(s) 270, 973
BfuAI ACCTGC 1 cut(s) 32
BisI GCNGC 9 cut(s) 156, 159, 180, 183, 270, 393, 448, 906, 909
BlsI GCNGC 9 cut(s) 157, 160, 181, 184, 271, 394, 449, 907, 910
BmcAI AGTACT 1 cut(s) 689
Bme1390I CCNGG 6 cut(s) 258, 337, 418, 441, 731, 824
Bme18I GGWCC 1 cut(s) 820
BmeT110I CYCGRG 1 cut(s) 396
BmgT120I GGNCC 2 cut(s) 820, 978
BmiI GGNNCC 2 cut(s) 875, 928
BmrFI CCNGG 6 cut(s) 258, 337, 418, 441, 731, 824
BmsI GCATC 3 cut(s) 220, 242, 490
BpiI GAAGAC 1 cut(s) 533
BpuMI CCSGG 4 cut(s) 258, 418, 441, 731
BsaHI GRCGYC 2 cut(s) 72, 300
BsaI GGTCTC 2 cut(s) 61, 469
BsaJI CCNNGG 7 cut(s) 117, 257, 304, 330, 417, 439, 881
BsaWI WCCGGW 3 cut(s) 64, 102, 578
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 2 cut(s) 336, 736
Bse118I RCCGGY 1 cut(s) 64
BseAI TCCGGA 1 cut(s) 578
BseBI CCWGG 2 cut(s) 337, 824
BseDI CCNNGG 7 cut(s) 117, 257, 304, 330, 417, 439, 881
BseGI GGATG 1 cut(s) 877
BseLI CCNNNNNNNGG 2 cut(s) 336, 736
BseMII CTCAG 2 cut(s) 362, 654
BseSI GKGCMC 1 cut(s) 356
BseX3I CGGCCG 1 cut(s) 120
BseXI GCAGC 8 cut(s) 145, 166, 167, 169, 256, 379, 892, 895
BsgI GTGCAG 1 cut(s) 393
Bsh1285I CGRYCG 2 cut(s) 69, 123
BshFI GGCC 4 cut(s) 122, 335, 447, 980
BshTI ACCGGT 1 cut(s) 64
BsiEI CGRYCG 2 cut(s) 69, 123
BsiHKAI GWGCWC 1 cut(s) 586
BsiHKCI CYCGRG 1 cut(s) 396
BsiSI CCGG 7 cut(s) 65, 103, 257, 417, 441, 579, 731
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 2 cut(s) 336, 736
BsmAI GTCTC 6 cut(s) 61, 119, 370, 393, 469, 558
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 611
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 4 cut(s) 122, 335, 447, 980
Bso31I GGTCTC 2 cut(s) 61, 469
BsoBI CYCGRG 1 cut(s) 396
Bsp1286I GDGCHC 2 cut(s) 356, 586
Bsp13I TCCGGA 1 cut(s) 578
Bsp143I GATC 3 cut(s) 39, 166, 745
BspACI CCGC 3 cut(s) 414, 448, 868
BspANI GGCC 4 cut(s) 122, 335, 447, 980
BspCNI CTCAG 2 cut(s) 361, 655
BspEI TCCGGA 1 cut(s) 578
BspLI GGNNCC 2 cut(s) 875, 928
BspMAI CTGCAG 2 cut(s) 274, 977
BspMI ACCTGC 1 cut(s) 32
BspPI GGATC 2 cut(s) 161, 740
BspQI GCTCTTC 1 cut(s) 616
BspTNI GGTCTC 2 cut(s) 61, 469
BsrFI RCCGGY 1 cut(s) 64
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 7 cut(s) 117, 257, 304, 330, 417, 439, 881
BssMI GATC 3 cut(s) 39, 166, 745
BssNI GRCGYC 2 cut(s) 72, 300
BssT1I CCWWGG 2 cut(s) 304, 330
Bst2UI CCWGG 2 cut(s) 337, 824
Bst4CI ACNGT 3 cut(s) 364, 404, 463
Bst6I CTCTTC 1 cut(s) 616
BstACI GRCGYC 2 cut(s) 72, 300
BstC8I GCNNGC 2 cut(s) 267, 651
BstDEI CTNAG 2 cut(s) 348, 663
BstF5I GGATG 1 cut(s) 877
BstKTI GATC 3 cut(s) 42, 169, 748
BstMAI GTCTC 6 cut(s) 61, 119, 370, 393, 469, 558
BstMBI GATC 3 cut(s) 39, 166, 745
BstMCI CGRYCG 2 cut(s) 69, 123
BstMWI GCNNNNNNNGC 2 cut(s) 179, 306
BstNI CCWGG 2 cut(s) 337, 824
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 6 cut(s) 256, 335, 416, 439, 729, 822
BstSFI CTRYAG 2 cut(s) 270, 973
BstSLI GKGCMC 1 cut(s) 356
BstV1I GCAGC 8 cut(s) 145, 166, 167, 169, 256, 379, 892, 895
BstV2I GAAGAC 1 cut(s) 533
BstZI CGGCCG 1 cut(s) 120
BsuRI GGCC 4 cut(s) 122, 335, 447, 980
BtgZI GCGATG 2 cut(s) 243, 926
BtsCI GGATG 1 cut(s) 877
BtsI GCAGTG 2 cut(s) 604, 844
BtsIMutI CAGTG 5 cut(s) 356, 360, 409, 604, 844
BveI ACCTGC 1 cut(s) 32
Cac8I GCNNGC 2 cut(s) 267, 651
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 820, 978
CseI GACGC 2 cut(s) 80, 289
Csp6I GTAC 2 cut(s) 688, 865
CspAI ACCGGT 1 cut(s) 64
CviAII CATG 5 cut(s) 111, 206, 286, 565, 650
CviQI GTAC 2 cut(s) 688, 865
DdeI CTNAG 2 cut(s) 348, 663
DpnI GATC 3 cut(s) 41, 168, 747
DpnII GATC 3 cut(s) 39, 166, 745
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 1 cut(s) 120
EagI CGGCCG 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 616
EarI CTCTTC 1 cut(s) 616
Ecl136II GAGCTC 1 cut(s) 584
EclXI CGGCCG 1 cut(s) 120
Eco130I CCWWGG 2 cut(s) 304, 330
Eco24I GRGCYC 1 cut(s) 586
Eco31I GGTCTC 2 cut(s) 61, 469
Eco47I GGWCC 1 cut(s) 820
Eco52I CGGCCG 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 584
Eco57I CTGAAG 2 cut(s) 13, 545
Eco88I CYCGRG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 584
EcoRII CCWGG 2 cut(s) 335, 822
EcoT14I CCWWGG 2 cut(s) 304, 330
EcoT22I ATGCAT 2 cut(s) 235, 505
EcoT38I GRGCYC 1 cut(s) 586
ErhI CCWWGG 2 cut(s) 304, 330
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 5 cut(s) 114, 209, 289, 568, 653
FalI AAGNNNNNCTT 2 cut(s) 518, 550
FaqI GGGAC 1 cut(s) 611
FatI CATG 5 cut(s) 110, 205, 285, 564, 649
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 9 cut(s) 156, 159, 180, 183, 270, 393, 448, 906, 909
FokI GGATG 1 cut(s) 864
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 9 cut(s) 156, 159, 180, 183, 270, 393, 448, 906, 909
FspBI CTAG 2 cut(s) 557, 849
GluI GCNGC 9 cut(s) 156, 159, 180, 183, 270, 393, 448, 906, 909
HaeIII GGCC 4 cut(s) 122, 335, 447, 980
HapII CCGG 7 cut(s) 65, 103, 257, 417, 441, 579, 731
HgaI GACGC 2 cut(s) 80, 289
Hin1I GRCGYC 2 cut(s) 72, 300
Hin1II CATG 5 cut(s) 114, 209, 289, 568, 653
HincII GTYRAC 2 cut(s) 70, 727
HindII GTYRAC 2 cut(s) 70, 727
HinfI GANTC 4 cut(s) 195, 248, 599, 796
HpaII CCGG 7 cut(s) 65, 103, 257, 417, 441, 579, 731
HphI GGTGA 7 cut(s) 13, 54, 376, 403, 580, 805, 928
Hpy166II GTNNAC 2 cut(s) 70, 727
Hpy188I TCNGA 4 cut(s) 194, 478, 820, 882
Hpy188III TCNNGA 2 cut(s) 557, 579
Hpy8I GTNNAC 2 cut(s) 70, 727
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 3 cut(s) 145, 397, 625
HpyCH4III ACNGT 3 cut(s) 364, 404, 463
HpyCH4V TGCA 8 cut(s) 161, 233, 272, 374, 503, 539, 653, 975
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 306
HpyF3I CTNAG 2 cut(s) 348, 663
Hsp92I GRCGYC 2 cut(s) 72, 300
Hsp92II CATG 5 cut(s) 114, 209, 289, 568, 653
Kpn2I TCCGGA 1 cut(s) 578
Kzo9I GATC 3 cut(s) 39, 166, 745
LguI GCTCTTC 1 cut(s) 616
LmnI GCTCC 3 cut(s) 581, 826, 834
Lsp1109I GCAGC 8 cut(s) 145, 166, 167, 169, 256, 379, 892, 895
LweI GCATC 3 cut(s) 220, 242, 490
MaeI CTAG 2 cut(s) 557, 849
MaeIII GTNAC 6 cut(s) 60, 452, 481, 568, 797, 839
MalI GATC 3 cut(s) 41, 168, 747
MboI GATC 3 cut(s) 39, 166, 745
MboII GAAGA 7 cut(s) 160, 499, 538, 603, 865, 931, 979
MhlI GDGCHC 2 cut(s) 356, 586
MluCI AATT 3 cut(s) 237, 787, 985
MlyI GAGTC 3 cut(s) 189, 593, 805
Mph1103I ATGCAT 2 cut(s) 235, 505
MroI TCCGGA 1 cut(s) 578
MseI TTAA 3 cut(s) 639, 845, 988
MslI CAYNNNNRTG 2 cut(s) 204, 935
MspA1I CMGCKG 2 cut(s) 158, 269
MspI CCGG 7 cut(s) 65, 103, 257, 417, 441, 579, 731
MspR9I CCNGG 6 cut(s) 258, 337, 418, 441, 731, 824
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 2 cut(s) 337, 824
MwoI GCNNNNNNNGC 2 cut(s) 179, 306
NciI CCSGG 4 cut(s) 258, 418, 441, 731
NdeII GATC 3 cut(s) 39, 166, 745
NlaIII CATG 5 cut(s) 114, 209, 289, 568, 653
NlaIV GGNNCC 2 cut(s) 875, 928
NmeAIII GCCGAG 2 cut(s) 98, 148
NmuCI GTSAC 6 cut(s) 60, 452, 481, 568, 797, 839
NsiI ATGCAT 2 cut(s) 235, 505
NspI RCATGY 1 cut(s) 653
PaeI GCATGC 1 cut(s) 653
PaqCI CACCTGC 1 cut(s) 32
PciSI GCTCTTC 1 cut(s) 616
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 1 cut(s) 248
PinAI ACCGGT 1 cut(s) 64
PkrI GCNGC 9 cut(s) 157, 160, 181, 184, 271, 394, 449, 907, 910
PleI GAGTC 3 cut(s) 189, 593, 804
PpsI GAGTC 3 cut(s) 189, 593, 804
Psp124BI GAGCTC 1 cut(s) 586
Psp6I CCWGG 2 cut(s) 335, 822
PspGI CCWGG 2 cut(s) 335, 822
PspN4I GGNNCC 2 cut(s) 875, 928
PspPI GGNCC 2 cut(s) 820, 978
PstI CTGCAG 2 cut(s) 274, 977
PvuII CAGCTG 2 cut(s) 158, 269
RsaI GTAC 2 cut(s) 689, 866
RsaNI GTAC 2 cut(s) 688, 865
RseI CAYNNNNRTG 2 cut(s) 204, 935
SacI GAGCTC 1 cut(s) 586
SalI GTCGAC 1 cut(s) 68
SapI GCTCTTC 1 cut(s) 616
SaqAI TTAA 3 cut(s) 639, 845, 988
SatI GCNGC 9 cut(s) 156, 159, 180, 183, 270, 393, 448, 906, 909
Sau3AI GATC 3 cut(s) 39, 166, 745
Sau96I GGNCC 2 cut(s) 820, 978
ScaI AGTACT 1 cut(s) 689
SchI GAGTC 3 cut(s) 189, 593, 805
ScrFI CCNGG 6 cut(s) 258, 337, 418, 441, 731, 824
SduI GDGCHC 2 cut(s) 356, 586
SfaNI GCATC 3 cut(s) 220, 242, 490
SfcI CTRYAG 2 cut(s) 270, 973
SinI GGWCC 1 cut(s) 820
SmiMI CAYNNNNRTG 2 cut(s) 204, 935
SphI GCATGC 1 cut(s) 653
Sse9I AATT 3 cut(s) 237, 787, 985
SsiI CCGC 3 cut(s) 414, 448, 868
SspMI CTAG 2 cut(s) 557, 849
SstI GAGCTC 1 cut(s) 586
StyD4I CCNGG 6 cut(s) 256, 335, 416, 439, 729, 822
StyI CCWWGG 2 cut(s) 304, 330
TaaI ACNGT 3 cut(s) 364, 404, 463
TaqI TCGA 1 cut(s) 69
TasI AATT 3 cut(s) 237, 787, 985
TatI WGTACW 1 cut(s) 687
TauI GCSGC 1 cut(s) 450
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 3 cut(s) 639, 845, 988
Tru9I TTAA 3 cut(s) 639, 845, 988
TscAI CASTG 5 cut(s) 356, 367, 409, 611, 844
TseFI GTSAC 6 cut(s) 60, 452, 481, 568, 797, 839
TseI GCWGC 8 cut(s) 155, 158, 179, 182, 269, 392, 905, 908
Tsp45I GTSAC 6 cut(s) 60, 452, 481, 568, 797, 839
TspDTI ATGAA 1 cut(s) 4
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 5 cut(s) 356, 367, 409, 611, 844
VpaK11BI GGWCC 1 cut(s) 820
XapI RAATTY 1 cut(s) 787
XbaI TCTAGA 1 cut(s) 556
XceI RCATGY 1 cut(s) 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 2 cut(s) 557, 849
ZrmI AGTACT 1 cut(s) 689
Zsp2I ATGCAT 2 cut(s) 235, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.