Rw4G021250

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014971
Physical Location & Seq
Forward (+)
45903108 .. 45904768
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G021250.1

Sequence Viewer

Length: 756 bp
ATGGCGTACTCCGCCGCCCGGCGCACATTAGCCTCCGCCCTCACGCGAACCCTAACCTCAACTTCCACTCGTTCAAACCCATCGTTCTCCTCCCGCTCTCGCTTCGCTTTGGCTCTTCTCAACAACCAACAACCCCAGCTCGTTCCCGACCCGGGTCACCTCCCGACCCGCTCCAAGCATTCCGGGTCGGGTTACTCGCCCCTCAACGACGCCACTCCCAACTGGAACAACCGCCCGCCCAAGGAGACCATCCTCCTCGACGGCTGCGACTACGAGCACTGGCTCATCATCCTCGAGTTCAACGACCCAAAGCCCTCGGAGGAGGAGATGATCAACAGCTACGTCAAAACCCTAGCTTCGGTCGTCGGAAGTGAGGAGGAGGCCAAGAAGAAGATATACTCGGTTTCGACGTCGACGTATACTGGATTTGGGGCTTTGATTTCTGAAGAGCTTTCTCTCAAGGTTAAAGAGCTGCCGGGTGTTCTCTGGGTTTTGCCGGATTCGTACCTTGATGTGCCCAACAAAGATTATGGAGGGGATCTATATATCGATGGGAAAGTCATCCCTAGGCCCCAGTATAGGTACACTGAGAGGCAAAACAACAGTCGAAACAGGCCTCGCCCACGACATGATAGGCGCCGGGAAACAATGCAGGTTGAAAGAAGACAGCCGGTGCAAAGACAGAACATGGTTCAGGATCAGAGAGGGCCTGTGCAGCAACCATCCCAAGAAGAGTTCAACAAGAGGGATGTTTAA

Protein Analysis

251

Amino Acids

28.69

Weight (kDa)

9.23

Isoelectric Point (pI)

61.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 91 - 180 3.9e-37 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014971)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06790 AT3G06790
fragaria_vesca FvH4_4g18760
malus_domestica MD16G1163100.v1.1
prunus_persica Prupe.1G130500_v2.0.a1
pyrus_communis pycom16g13820
rosa_chinensis RchiOBHm_Chr4g0423551
rosa_laevigata RLG00000007548
rosa_multiflora Rmu_co8017616.1_g000001
rosa_roxburghii Rroxscaffold_5G00365780
rosa_rugosa Rorug04G0188600
rosa_samantha Rh4AG248100 Rh4BG253300 Rh4CG261300 Rh4DG248600
rosa_wichuraiana Rw4G021250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 413
Acc36I ACCTGC 1 cut(s) 643
AccB1I GGYRCC 1 cut(s) 636
AccBSI CCGCTC 2 cut(s) 96, 171
AccI GTMKAC 2 cut(s) 413, 419
AccII CGCG 1 cut(s) 46
AciI CCGC 7 cut(s) 12, 15, 36, 94, 169, 232, 236
AclWI GGATC 2 cut(s) 546, 705
AcuI CTGAAG 1 cut(s) 465
AcyI GRCGYC 3 cut(s) 210, 410, 637
AfaI GTAC 3 cut(s) 8, 506, 584
AfiI CCNNNNNNNGG 5 cut(s) 18, 152, 241, 358, 579
AgsI TTSAA 4 cut(s) 75, 301, 659, 739
AluBI AGCT 5 cut(s) 139, 339, 356, 451, 472
AluI AGCT 5 cut(s) 139, 339, 356, 451, 472
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 2 cut(s) 546, 705
Ama87I CYCGRG 2 cut(s) 151, 293
AoxI GGCC 4 cut(s) 381, 569, 614, 707
ApeKI GCWGC 3 cut(s) 264, 472, 715
ArsI GACNNNNNNTTYG 2 cut(s) 589, 621
AspA2I CCTAGG 1 cut(s) 566
AspLEI GCGC 2 cut(s) 24, 639
AspS9I GGNCC 2 cut(s) 570, 707
AsuC2I CCSGG 6 cut(s) 19, 152, 153, 184, 477, 641
AsuHPI GGTGA 1 cut(s) 149
AvaI CYCGRG 2 cut(s) 151, 293
AvrII CCTAGG 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 519
BanI GGYRCC 1 cut(s) 636
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
BbsI GAAGAC 1 cut(s) 670
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 3 cut(s) 251, 459, 727
BccI CCATC 4 cut(s) 88, 257, 545, 730
BceAI ACGGC 1 cut(s) 277
BclI TGATCA 1 cut(s) 330
BcnI CCSGG 6 cut(s) 19, 152, 153, 184, 477, 641
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 2 cut(s) 353, 567
BfoI RGCGCY 1 cut(s) 640
BfuAI ACCTGC 1 cut(s) 643
BisI GCNGC 4 cut(s) 15, 265, 473, 716
BlnI CCTAGG 1 cut(s) 566
BlsI GCNGC 4 cut(s) 16, 266, 474, 717
Bme1390I CCNGG 6 cut(s) 19, 152, 153, 184, 477, 641
BmeT110I CYCGRG 2 cut(s) 151, 293
BmgT120I GGNCC 2 cut(s) 570, 707
BmiI GGNNCC 2 cut(s) 572, 638
BmrFI CCNGG 6 cut(s) 19, 152, 153, 184, 477, 641
BmrI ACTGGG 1 cut(s) 568
BmuI ACTGGG 1 cut(s) 568
BoxI GACNNNNGTC 1 cut(s) 153
BpiI GAAGAC 1 cut(s) 670
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 6 cut(s) 19, 152, 153, 184, 477, 641
Bsa29I ATCGAT 1 cut(s) 549
BsaHI GRCGYC 3 cut(s) 210, 410, 637
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 4 cut(s) 151, 240, 315, 566
Bsc4I CCNNNNNNNGG 5 cut(s) 18, 152, 241, 358, 579
Bse118I RCCGGY 1 cut(s) 670
Bse1I ACTGG 4 cut(s) 227, 284, 427, 574
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 4 cut(s) 151, 240, 315, 566
BseGI GGATG 5 cut(s) 249, 288, 561, 722, 754
BseLI CCNNNNNNNGG 5 cut(s) 18, 152, 241, 358, 579
BseMII CTCAG 1 cut(s) 579
BseNI ACTGG 4 cut(s) 227, 284, 427, 574
BseRI GAGGAG 6 cut(s) 79, 245, 335, 338, 389, 392
BseSI GKGCMC 1 cut(s) 519
BseXI GCAGC 3 cut(s) 251, 459, 727
BseYI CCCAGC 1 cut(s) 135
BsgI GTGCAG 1 cut(s) 734
Bsh1236I CGCG 1 cut(s) 46
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 4 cut(s) 383, 571, 616, 709
BshNI GGYRCC 1 cut(s) 636
BshVI ATCGAT 1 cut(s) 549
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 2 cut(s) 151, 293
BsiSI CCGG 7 cut(s) 19, 152, 183, 476, 497, 640, 671
BslI CCNNNNNNNGG 5 cut(s) 18, 152, 241, 358, 579
BsmAI GTCTC 1 cut(s) 239
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 4 cut(s) 383, 571, 616, 709
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 2 cut(s) 151, 293
Bsp1286I GDGCHC 2 cut(s) 279, 519
Bsp143I GATC 3 cut(s) 330, 538, 697
BspACI CCGC 7 cut(s) 12, 15, 36, 94, 169, 232, 236
BspANI GGCC 4 cut(s) 383, 571, 616, 709
BspCNI CTCAG 1 cut(s) 580
BspDI ATCGAT 1 cut(s) 549
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 572, 638
BspMI ACCTGC 1 cut(s) 643
BspPI GGATC 2 cut(s) 546, 705
BspQI GCTCTTC 2 cut(s) 120, 441
BspT107I GGYRCC 1 cut(s) 636
BspTNI GGTCTC 1 cut(s) 239
BsrBI CCGCTC 2 cut(s) 96, 171
BsrFI RCCGGY 1 cut(s) 670
BsrI ACTGG 4 cut(s) 227, 284, 427, 574
BssAI RCCGGY 1 cut(s) 670
BssECI CCNNGG 4 cut(s) 151, 240, 315, 566
BssMI GATC 3 cut(s) 330, 538, 697
BssNAI GTATAC 1 cut(s) 420
BssNI GRCGYC 3 cut(s) 210, 410, 637
BssT1I CCWWGG 2 cut(s) 240, 566
Bst1107I GTATAC 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 605
Bst6I CTCTTC 3 cut(s) 120, 441, 726
BstACI GRCGYC 3 cut(s) 210, 410, 637
BstC8I GCNNGC 1 cut(s) 236
BstDEI CTNAG 1 cut(s) 588
BstEII GGTNACC 1 cut(s) 155
BstF5I GGATG 5 cut(s) 249, 288, 561, 722, 754
BstFNI CGCG 1 cut(s) 46
BstH2I RGCGCY 1 cut(s) 640
BstHHI GCGC 2 cut(s) 24, 639
BstKTI GATC 3 cut(s) 333, 541, 700
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 3 cut(s) 330, 538, 697
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 2 cut(s) 11, 715
BstPAI GACNNNNGTC 1 cut(s) 153
BstPI GGTNACC 1 cut(s) 155
BstSCI CCNGG 6 cut(s) 17, 150, 151, 182, 475, 639
BstSLI GKGCMC 1 cut(s) 519
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 3 cut(s) 251, 459, 727
BstV2I GAAGAC 1 cut(s) 670
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
BstZ17I GTATAC 1 cut(s) 420
Bsu15I ATCGAT 1 cut(s) 549
BsuRI GGCC 4 cut(s) 383, 571, 616, 709
BsuTUI ATCGAT 1 cut(s) 549
BtsCI GGATG 5 cut(s) 249, 288, 561, 722, 754
BtsIMutI CAGTG 2 cut(s) 277, 585
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 1 cut(s) 236
CfoI GCGC 2 cut(s) 24, 639
Cfr10I RCCGGY 1 cut(s) 670
Cfr13I GGNCC 2 cut(s) 570, 707
Cfr9I CCCGGG 1 cut(s) 151
ClaI ATCGAT 1 cut(s) 549
CseI GACGC 1 cut(s) 218
Csp6I GTAC 3 cut(s) 7, 505, 583
CviAII CATG 2 cut(s) 629, 688
CviQI GTAC 3 cut(s) 7, 505, 583
DdeI CTNAG 1 cut(s) 588
DinI GGCGCC 1 cut(s) 638
DpnI GATC 3 cut(s) 332, 540, 699
DpnII GATC 3 cut(s) 330, 538, 697
Eam1104I CTCTTC 3 cut(s) 120, 441, 726
EarI CTCTTC 3 cut(s) 120, 441, 726
EciI GGCGGA 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 240, 566
Eco147I AGGCCT 1 cut(s) 616
Eco31I GGTCTC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 465
Eco88I CYCGRG 2 cut(s) 151, 293
Eco91I GGTNACC 1 cut(s) 155
EcoO109I RGGNCCY 2 cut(s) 570, 707
EcoO65I GGTNACC 1 cut(s) 155
EcoT14I CCWWGG 2 cut(s) 240, 566
EgeI GGCGCC 1 cut(s) 638
EheI GGCGCC 1 cut(s) 638
ErhI CCWWGG 2 cut(s) 240, 566
FaeI CATG 2 cut(s) 632, 691
FaiI YATR 8 cut(s) 397, 420, 531, 544, 546, 579, 630, 689
FatI CATG 2 cut(s) 628, 687
FauI CCCGC 3 cut(s) 101, 176, 243
FbaI TGATCA 1 cut(s) 330
FblI GTMKAC 2 cut(s) 413, 419
Fnu4HI GCNGC 4 cut(s) 15, 265, 473, 716
FokI GGATG 4 cut(s) 236, 275, 548, 709
Fsp4HI GCNGC 4 cut(s) 15, 265, 473, 716
FspBI CTAG 2 cut(s) 353, 567
GlaI GCGC 2 cut(s) 23, 638
GluI GCNGC 4 cut(s) 15, 265, 473, 716
GsaI CCCAGC 1 cut(s) 139
HaeII RGCGCY 1 cut(s) 640
HaeIII GGCC 4 cut(s) 383, 571, 616, 709
HapII CCGG 7 cut(s) 19, 152, 183, 476, 497, 640, 671
HgaI GACGC 1 cut(s) 218
HhaI GCGC 2 cut(s) 24, 639
Hin1I GRCGYC 3 cut(s) 210, 410, 637
Hin1II CATG 2 cut(s) 632, 691
Hin6I GCGC 2 cut(s) 22, 637
HinP1I GCGC 2 cut(s) 22, 637
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 1 cut(s) 500
HpaII CCGG 7 cut(s) 19, 152, 183, 476, 497, 640, 671
HphI GGTGA 1 cut(s) 149
Hpy166II GTNNAC 3 cut(s) 414, 420, 585
Hpy188I TCNGA 4 cut(s) 319, 368, 445, 702
Hpy188III TCNNGA 3 cut(s) 146, 163, 695
Hpy8I GTNNAC 3 cut(s) 414, 420, 585
Hpy99I CGWCG 6 cut(s) 212, 263, 368, 412, 415, 418
HpyCH4III ACNGT 1 cut(s) 605
HpyCH4IV ACGT 3 cut(s) 342, 410, 416
HpyCH4V TGCA 3 cut(s) 652, 676, 715
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 715
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 3 cut(s) 342, 410, 416
Hsp92I GRCGYC 3 cut(s) 210, 410, 637
Hsp92II CATG 2 cut(s) 632, 691
HspAI GCGC 2 cut(s) 22, 637
KasI GGCGCC 1 cut(s) 636
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 3 cut(s) 330, 538, 697
LguI GCTCTTC 2 cut(s) 120, 441
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 3 cut(s) 251, 459, 727
MaeI CTAG 2 cut(s) 353, 567
MaeII ACGT 3 cut(s) 342, 410, 416
MaeIII GTNAC 2 cut(s) 155, 191
MalI GATC 3 cut(s) 332, 540, 699
MbiI CCGCTC 2 cut(s) 96, 171
MboI GATC 3 cut(s) 330, 538, 697
MboII GAAGA 6 cut(s) 107, 400, 403, 458, 675, 743
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 2 cut(s) 279, 519
Mly113I GGCGCC 1 cut(s) 637
MmeI TCCRAC 1 cut(s) 346
MseI TTAA 2 cut(s) 465, 754
MspI CCGG 7 cut(s) 19, 152, 183, 476, 497, 640, 671
MspR9I CCNGG 6 cut(s) 19, 152, 153, 184, 477, 641
Mva1269I GAATGC 1 cut(s) 178
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 11, 715
NarI GGCGCC 1 cut(s) 637
NciI CCSGG 6 cut(s) 19, 152, 153, 184, 477, 641
NdeII GATC 3 cut(s) 330, 538, 697
NlaIII CATG 2 cut(s) 632, 691
NlaIV GGNNCC 2 cut(s) 572, 638
NmuCI GTSAC 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 293
PceI AGGCCT 1 cut(s) 616
PciSI GCTCTTC 2 cut(s) 120, 441
PcsI WCGNNNNNNNCGW 4 cut(s) 264, 300, 407, 413
PctI GAATGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 500
PkrI GCNGC 4 cut(s) 16, 266, 474, 717
PluTI GGCGCC 1 cut(s) 640
PshAI GACNNNNGTC 1 cut(s) 153
PspEI GGTNACC 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 572, 638
PspPI GGNCC 2 cut(s) 570, 707
PspXI VCTCGAGB 1 cut(s) 293
PsuI RGATCY 1 cut(s) 538
RsaI GTAC 3 cut(s) 8, 506, 584
RsaNI GTAC 3 cut(s) 7, 505, 583
SalI GTCGAC 1 cut(s) 412
SapI GCTCTTC 2 cut(s) 120, 441
SaqAI TTAA 2 cut(s) 465, 754
SatI GCNGC 4 cut(s) 15, 265, 473, 716
Sau3AI GATC 3 cut(s) 330, 538, 697
Sau96I GGNCC 2 cut(s) 570, 707
ScrFI CCNGG 6 cut(s) 19, 152, 153, 184, 477, 641
SduI GDGCHC 2 cut(s) 279, 519
SfoI GGCGCC 1 cut(s) 638
Sfr274I CTCGAG 1 cut(s) 293
SgrDI CGTCGACG 1 cut(s) 412
SlaI CTCGAG 1 cut(s) 293
SmaI CCCGGG 1 cut(s) 153
SmlI CTYRAG 2 cut(s) 293, 458
SmoI CTYRAG 2 cut(s) 293, 458
SseBI AGGCCT 1 cut(s) 616
SsiI CCGC 7 cut(s) 12, 15, 36, 94, 169, 232, 236
SspDI GGCGCC 1 cut(s) 636
SspMI CTAG 2 cut(s) 353, 567
StuI AGGCCT 1 cut(s) 616
StyD4I CCNGG 6 cut(s) 17, 150, 151, 182, 475, 639
StyI CCWWGG 2 cut(s) 240, 566
TaaI ACNGT 1 cut(s) 605
TaiI ACGT 3 cut(s) 345, 413, 419
TaqI TCGA 6 cut(s) 258, 294, 407, 413, 549, 607
TaqII GACCGA 1 cut(s) 349
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 2 cut(s) 465, 754
Tru9I TTAA 2 cut(s) 465, 754
TscAI CASTG 2 cut(s) 284, 592
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 3 cut(s) 264, 472, 715
Tsp45I GTSAC 1 cut(s) 155
TspMI CCCGGG 1 cut(s) 151
TspRI CASTG 2 cut(s) 284, 592
XhoI CTCGAG 1 cut(s) 293
XmaI CCCGGG 1 cut(s) 151
XmaJI CCTAGG 1 cut(s) 566
XmiI GTMKAC 2 cut(s) 413, 419
XspI CTAG 2 cut(s) 353, 567
ZraI GACGTC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.