Rorug04G0188600

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008628
Physical Location & Seq
Forward (+)
32788346 .. 32793666
5321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0188600.1

Sequence Viewer

Length: 909 bp
ATGGCTCGTAATGAAGAGAAAGCGCAGTCCATGCTCAATCGCTACATCACTCTCAAAAACGAAGAGAAGAAGAAGCCCCGAGAGCGCCGCCCTTTCCTCGCCTCCGAATGCCGCGACCTCGCCGAGGCCTCCAAGTGGCGCCAGCAGATCATGGGAGAGATCGGCCGCAAAGTCGCCGAGATCCAAAACGAAGGCCTTGGCGAGCACCGCCTCCGCGACCTCAACGACGAGATCAACAAGCTCATCCGCGAGAAGACTCACTGGGAGCGCCGCATCGTTGAGCTCGGCGGCCCCAATTATGCCAAGCACGCCCCTAAGATGACCGATCTCGACGGCAACATCGTTGACGTTCCGAACCCTAGCGGCCGCGGCGCCGGTTACCGGTACTTTGGCGCGGCGAAGAAGCTGCCAGGTGTCAGGGAGCTTTTCGAGAAGCCGCCGGAGCTGAGGAAGCGGCGGACGAGGTATGACATTTACAAGAGGATTGATGCCAGCTATTATGGGTACAGGGACGACGAGGATGGGGTTTTGGAGAAGCTAGAGGTTGTGGCGGAGGAGAAGATGAGGGCGGAGGCGGTGGCAGAGTGGTGGAGGTTGGAGGAGATAAGGAGAGAGGCCAAGAGGGCCGTGAAGAGTGGTGAAGTGGTGACGGTGGCGAAGGCGAAGGAGATTCTGGTGGAGGAGGAGGAGGAGGTGGTGGAAGAAGAGAGGCAGAGAGAGAGGGAGTTATTGAAAGAGAAGAGTGAAAAAGAGATGGAGTTTGTGGCTCATGTGCCGCTGCCGGATGACAAGGAGATTGAGAAGATGGTGCTTGAGAAGAAGAAGAAGGACTTGCTGAATAGGTATGTCAGTGAGGAATTGATGCAGGACCAGACTGAGGCTAAGCAGATGCTTAACATTAAGCGGTAG

Protein Analysis

302

Amino Acids

35.69

Weight (kDa)

6.97

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isy1 PF06246 1 - 281 5e-91 Isy1-like splicing family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 138, 371
AccII CGCG 5 cut(s) 114, 216, 249, 369, 395
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 2 cut(s) 163, 364
AcyI GRCGYC 2 cut(s) 139, 372
AfaI GTAC 2 cut(s) 386, 506
AfiI CCNNNNNNNGG 4 cut(s) 124, 135, 381, 877
AgeI ACCGGT 1 cut(s) 381
AgsI TTSAA 1 cut(s) 733
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 7 cut(s) 241, 283, 406, 424, 445, 495, 538
AluI AGCT 7 cut(s) 241, 283, 406, 424, 445, 495, 538
Alw21I GWGCWC 2 cut(s) 207, 285
AlwI GGATC 1 cut(s) 175
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 7 cut(s) 126, 163, 193, 289, 364, 615, 624
ApeKI GCWGC 2 cut(s) 406, 778
AsiGI ACCGGT 1 cut(s) 381
AspLEI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
AspS9I GGNCC 3 cut(s) 290, 624, 868
AsuHPI GGTGA 2 cut(s) 650, 658
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 1 cut(s) 868
BanI GGYRCC 2 cut(s) 138, 371
BanII GRGCYC 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 2 cut(s) 207, 285
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 2 cut(s) 393, 765
BccI CCATC 3 cut(s) 515, 748, 799
BceAI ACGGC 2 cut(s) 349, 611
BcgI CGANNNNNNTGC 2 cut(s) 388, 422
BciT130I CCWGG 1 cut(s) 411
BfaI CTAG 2 cut(s) 360, 539
BfoI RGCGCY 4 cut(s) 88, 142, 271, 375
BglI GCCNNNNNGGC 1 cut(s) 623
BlpI GCTNAGC 1 cut(s) 882
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 1 cut(s) 868
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 3 cut(s) 290, 624, 868
BmiI GGNNCC 3 cut(s) 140, 292, 373
BmrFI CCNGG 1 cut(s) 411
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 4 cut(s) 282, 478, 852, 879
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 446
Bpu1102I GCTNAGC 1 cut(s) 882
BpuEI CTTGAG 1 cut(s) 833
BsaHI GRCGYC 2 cut(s) 139, 372
BsaJI CCNNGG 3 cut(s) 123, 196, 367
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 135, 381, 877
Bse118I RCCGGY 2 cut(s) 374, 381
Bse1I ACTGG 1 cut(s) 266
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 3 cut(s) 123, 196, 367
BseGI GGATG 3 cut(s) 243, 526, 790
BseLI CCNNNNNNNGG 4 cut(s) 124, 135, 381, 877
BseMII CTCAG 2 cut(s) 437, 867
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 6 cut(s) 569, 614, 695, 698, 701, 704
BseX3I CGGCCG 2 cut(s) 163, 364
BseXI GCAGC 2 cut(s) 393, 765
Bsh1236I CGCG 5 cut(s) 114, 216, 249, 369, 395
Bsh1285I CGRYCG 2 cut(s) 166, 367
BshFI GGCC 7 cut(s) 128, 165, 195, 291, 366, 617, 626
BshNI GGYRCC 2 cut(s) 138, 371
BshTI ACCGGT 1 cut(s) 381
BsiEI CGRYCG 2 cut(s) 166, 367
BsiHKAI GWGCWC 2 cut(s) 207, 285
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 4 cut(s) 375, 382, 440, 782
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 4 cut(s) 124, 135, 381, 877
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 7 cut(s) 128, 165, 195, 291, 366, 617, 626
BsoBI CYCGRG 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 207, 285
Bsp143I GATC 5 cut(s) 147, 159, 180, 231, 325
Bsp1720I GCTNAGC 1 cut(s) 882
BspANI GGCC 7 cut(s) 128, 165, 195, 291, 366, 617, 626
BspCNI CTCAG 2 cut(s) 438, 868
BspFNI CGCG 5 cut(s) 114, 216, 249, 369, 395
BspLI GGNNCC 3 cut(s) 140, 292, 373
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 2 cut(s) 138, 371
BsrFI RCCGGY 2 cut(s) 374, 381
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 2 cut(s) 374, 381
BssECI CCNNGG 3 cut(s) 123, 196, 367
BssMI GATC 5 cut(s) 147, 159, 180, 231, 325
BssNI GRCGYC 2 cut(s) 139, 372
BssT1I CCWWGG 1 cut(s) 196
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 1 cut(s) 652
Bst6I CTCTTC 5 cut(s) 9, 57, 626, 699, 734
BstACI GRCGYC 2 cut(s) 139, 372
BstAPI GCANNNNNTGC 1 cut(s) 31
BstC8I GCNNGC 4 cut(s) 143, 203, 309, 493
BstDEI CTNAG 4 cut(s) 315, 446, 876, 882
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 3 cut(s) 243, 526, 790
BstFNI CGCG 5 cut(s) 114, 216, 249, 369, 395
BstH2I RGCGCY 4 cut(s) 88, 142, 271, 375
BstHHI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
BstKTI GATC 5 cut(s) 150, 162, 183, 234, 328
BstMBI GATC 5 cut(s) 147, 159, 180, 231, 325
BstMCI CGRYCG 2 cut(s) 166, 367
BstMWI GCNNNNNNNGC 8 cut(s) 31, 82, 207, 308, 369, 442, 451, 623
BstNI CCWGG 1 cut(s) 411
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 409
BstUI CGCG 5 cut(s) 114, 216, 249, 369, 395
BstV1I GCAGC 2 cut(s) 393, 765
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BstZI CGGCCG 2 cut(s) 163, 364
BsuRI GGCC 7 cut(s) 128, 165, 195, 291, 366, 617, 626
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 243, 526, 790
BtsIMutI CAGTG 2 cut(s) 259, 856
Cac8I GCNNGC 4 cut(s) 143, 203, 309, 493
CciNI GCGGCCGC 1 cut(s) 364
CfoI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
Cfr10I RCCGGY 2 cut(s) 374, 381
Cfr13I GGNCC 3 cut(s) 290, 624, 868
Cfr42I CCGCGG 1 cut(s) 370
Csp6I GTAC 2 cut(s) 385, 505
CspAI ACCGGT 1 cut(s) 381
CviAII CATG 3 cut(s) 31, 151, 770
CviQI GTAC 2 cut(s) 385, 505
DdeI CTNAG 4 cut(s) 315, 446, 876, 882
DinI GGCGCC 2 cut(s) 140, 373
DpnI GATC 5 cut(s) 149, 161, 182, 233, 327
DpnII GATC 5 cut(s) 147, 159, 180, 231, 325
EaeI YGGCCR 2 cut(s) 163, 364
EagI CGGCCG 2 cut(s) 163, 364
Eam1104I CTCTTC 5 cut(s) 9, 57, 626, 699, 734
EarI CTCTTC 5 cut(s) 9, 57, 626, 699, 734
EciI GGCGGA 3 cut(s) 472, 566, 584
Ecl136II GAGCTC 1 cut(s) 283
EclXI CGGCCG 2 cut(s) 163, 364
Eco130I CCWWGG 1 cut(s) 196
Eco147I AGGCCT 2 cut(s) 128, 195
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 868
Eco52I CGGCCG 2 cut(s) 163, 364
Eco53kI GAGCTC 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO65I GGTNACC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 196
EcoT38I GRGCYC 1 cut(s) 285
EgeI GGCGCC 2 cut(s) 140, 373
EheI GGCGCC 2 cut(s) 140, 373
ErhI CCWWGG 1 cut(s) 196
FaeI CATG 3 cut(s) 34, 154, 773
FaiI YATR 7 cut(s) 32, 152, 300, 468, 501, 771, 846
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 1 cut(s) 524
FatI CATG 3 cut(s) 30, 150, 769
FokI GGATG 3 cut(s) 230, 533, 797
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 2 cut(s) 360, 539
GlaI GCGC 6 cut(s) 24, 86, 140, 269, 373, 394
HaeII RGCGCY 4 cut(s) 88, 142, 271, 375
HaeIII GGCC 7 cut(s) 128, 165, 195, 291, 366, 617, 626
HapII CCGG 4 cut(s) 375, 382, 440, 782
HhaI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
Hin1I GRCGYC 2 cut(s) 139, 372
Hin1II CATG 3 cut(s) 34, 154, 773
Hin6I GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
HinP1I GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 2 cut(s) 256, 670
HpaII CCGG 4 cut(s) 375, 382, 440, 782
HphI GGTGA 2 cut(s) 650, 658
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 2 cut(s) 106, 354
Hpy188III TCNNGA 2 cut(s) 329, 430
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 3 cut(s) 230, 335, 518
HpyAV CCTTC 4 cut(s) 185, 652, 658, 820
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 1 cut(s) 865
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 82, 207, 308, 369, 442, 451, 623
HpyF3I CTNAG 4 cut(s) 315, 446, 876, 882
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 2 cut(s) 139, 372
Hsp92II CATG 3 cut(s) 34, 154, 773
HspAI GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
KasI GGCGCC 2 cut(s) 138, 371
KspI CCGCGG 1 cut(s) 370
Kzo9I GATC 5 cut(s) 147, 159, 180, 231, 325
LmnI GCTCC 3 cut(s) 265, 421, 442
Lsp1109I GCAGC 2 cut(s) 393, 765
LweI GCATC 4 cut(s) 282, 478, 852, 879
MaeI CTAG 2 cut(s) 360, 539
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 377, 646
MalI GATC 5 cut(s) 149, 161, 182, 233, 327
MboI GATC 5 cut(s) 147, 159, 180, 231, 325
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 2 cut(s) 207, 285
MluCI AATT 2 cut(s) 295, 857
Mly113I GGCGCC 2 cut(s) 139, 372
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 1 cut(s) 576
MseI TTAA 2 cut(s) 894, 900
MspA1I CMGCKG 2 cut(s) 369, 778
MspI CCGG 4 cut(s) 375, 382, 440, 782
MspR9I CCNGG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 113
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 5 cut(s) 114, 216, 249, 369, 395
MwoI GCNNNNNNNGC 8 cut(s) 31, 82, 207, 308, 369, 442, 451, 623
NarI GGCGCC 2 cut(s) 139, 372
NdeII GATC 5 cut(s) 147, 159, 180, 231, 325
NlaIII CATG 3 cut(s) 34, 154, 773
NlaIV GGNNCC 3 cut(s) 140, 292, 373
NmeAIII GCCGAG 3 cut(s) 148, 202, 264
NmuCI GTSAC 1 cut(s) 646
NotI GCGGCCGC 1 cut(s) 364
PceI AGGCCT 2 cut(s) 128, 195
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 670
PinAI ACCGGT 1 cut(s) 381
PleI GAGTC 1 cut(s) 250
PluTI GGCGCC 2 cut(s) 142, 375
PpsI GAGTC 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 409
PspEI GGTNACC 1 cut(s) 377
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 3 cut(s) 140, 292, 373
PspPI GGNCC 3 cut(s) 290, 624, 868
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 2 cut(s) 386, 506
RsaNI GTAC 2 cut(s) 385, 505
SacI GAGCTC 1 cut(s) 285
SacII CCGCGG 1 cut(s) 370
SaqAI TTAA 2 cut(s) 894, 900
Sau3AI GATC 5 cut(s) 147, 159, 180, 231, 325
Sau96I GGNCC 3 cut(s) 290, 624, 868
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 2 cut(s) 207, 285
SfaNI GCATC 4 cut(s) 282, 478, 852, 879
SfiI GGCCNNNNNGGCC 1 cut(s) 623
SfoI GGCGCC 2 cut(s) 140, 373
Sfr303I CCGCGG 1 cut(s) 370
SgrBI CCGCGG 1 cut(s) 370
SinI GGWCC 1 cut(s) 868
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
Sse9I AATT 2 cut(s) 295, 857
SseBI AGGCCT 2 cut(s) 128, 195
SspDI GGCGCC 2 cut(s) 138, 371
SspMI CTAG 2 cut(s) 360, 539
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 2 cut(s) 128, 195
StyD4I CCNGG 1 cut(s) 409
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 1 cut(s) 652
TaiI ACGT 1 cut(s) 351
TaqI TCGA 2 cut(s) 330, 429
TaqII GACCGA 1 cut(s) 338
TasI AATT 2 cut(s) 295, 857
TfiI GAWTC 1 cut(s) 670
Tru1I TTAA 2 cut(s) 894, 900
Tru9I TTAA 2 cut(s) 894, 900
TscAI CASTG 2 cut(s) 266, 856
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 2 cut(s) 406, 778
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 1 cut(s) 27
TspRI CASTG 2 cut(s) 266, 856
VpaK11BI GGWCC 1 cut(s) 868
XagI CCTNNNNNAGG 1 cut(s) 122
XspI CTAG 2 cut(s) 360, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.