MD16G1163200.v1.1

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008628
Physical Location & Seq
Reverse (-)
13388267 .. 13390575
2309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1163200.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGCCATTGCTGGAGGAATGTCTTGCCACGCCATTCTTGTCTTGTGCTTAGCTGCATTGGCCTCTCGAGCCACCGGCAGGGTTGCCATTCCCCCGGCTGTGTTCGATGTTTTCCGTGGTAGTAATTTGGGTGTCCATGACAAAGCTGATCCTGATGAGAAGATCTTTAACGTGGTAGATTTCGGCGCCAAGCCTGATGGGAACCATGATGCAAGCATGAATTTCATAAAAACATGGCTTGCAGCATGCCACAACACTGGGAAATCAAGGGTTCTGATCCCACCGGGGACATACAAGAGTGGGCCAGTGGTATTTCAGGGGCCATGCACCAGTTCCAAACCCGTAGTTGTTCAAGTTTTAGGGACCATAAAAGGCCTGAATGATCTGTCCTTATATGAAGAGCCATACTGGTTCTTGTTTGAGAATGTAGAAGGCTTGGTCATTATCGGCAATGGCATCTTTGACGGACAAGGTTCAAGTGCCTGGAAAGTCGCTGGTGACTGCGGCAGTTCCAGCGATGGTTGCAGTCCACTCCCATCTAACATCAAATTCAACGGCGTTAGAGACGGGGTCATTCGAGGCATTAGTTCCCTCAATAGCAAAGGAGTTCATCTTTTCATCACTGACAGCCAGAATGTTAGGGTTCGCAGAGTGAATATCTCTGCCCCTCGAACAAGCCCCAACACTGATGGCATCCACATCAGCAACTCCAACAATGTCAAGGTCGCCAGAACTCACATTGCCACCGGTGATGATTGCATTGGCATGATTCAGGGATCAACCAACATTGCCATCAACAATGTAATTTGTGGCCCCGGACACGGCATTAGTGTTGGTAGCCTTGGCAAGTATGAGCACGAAAATGACGTGACAGGAATCACCGTGAAGAAGACCACATTTTTGAACACAGACAATGGGATTAGAATCAAGTCATGGCCAGGATCGGGTCCTAGTCGGGCATCAGGAATGGTTTTCAAGGACATCATCATGCAGAATGTTAGGAACCCTATTATAATTGACCAAGAGTACTGCACTGGGGGATGCAACAAGAACCAGCCATCGCACGTGCAGATCAGCGATGTCCACTACATAAACATTAGAGGGACAACACCGTCAAATGTGGCAGTAGATTTCATTTGCAGCAGCCAGGTTCCTTGCCAAAACATTCAGCTTCACGACATCGATTTGAAGTATACTGGTCCAAATGCTGCAGCTCTCACCAACGTATGTAAAAATGTAAAAGCTGGTTTTCGTGGCATACAAAACCCACCAGCCTGCCACTGA

Protein Analysis

428

Amino Acids

45.39

Weight (kDa)

8.48

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 83 - 411 1.1e-84 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1013
AasI GACNNNNNNGTC 1 cut(s) 1111
AccB1I GGYRCC 1 cut(s) 185
AccI GTMKAC 1 cut(s) 1193
AciI CCGC 1 cut(s) 504
AclWI GGATC 4 cut(s) 143, 271, 784, 949
AcoI YGGCCR 2 cut(s) 3, 935
AcsI RAATTY 2 cut(s) 220, 548
AcvI CACGTG 1 cut(s) 1066
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 1028
AfiI CCNNNNNNNGG 3 cut(s) 78, 821, 944
AgeI ACCGGT 1 cut(s) 746
AgsI TTSAA 6 cut(s) 353, 477, 553, 904, 976, 1189
AjiI CACGTC 1 cut(s) 868
AjnI CCWGG 3 cut(s) 482, 937, 1146
AluBI AGCT 5 cut(s) 53, 146, 1171, 1214, 1244
AluI AGCT 5 cut(s) 53, 146, 1171, 1214, 1244
Alw21I GWGCWC 1 cut(s) 858
Alw26I GTCTC 1 cut(s) 558
AlwI GGATC 4 cut(s) 143, 271, 784, 949
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 7 cut(s) 3, 60, 302, 320, 373, 811, 935
ApeKI GCWGC 6 cut(s) 53, 242, 1140, 1143, 1208, 1211
ApoI RAATTY 2 cut(s) 220, 548
ArsI GACNNNNNNTTYG 2 cut(s) 883, 915
AsiGI ACCGGT 1 cut(s) 746
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
AsuC2I CCSGG 3 cut(s) 95, 285, 816
AsuHPI GGTGA 4 cut(s) 509, 761, 871, 1210
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 3 cut(s) 363, 947, 1199
BalI TGGCCA 2 cut(s) 5, 937
BanI GGYRCC 1 cut(s) 185
BbrPI CACGTG 1 cut(s) 1066
BbsI GAAGAC 1 cut(s) 896
Bbv12I GWGCWC 1 cut(s) 858
BbvI GCAGC 6 cut(s) 40, 254, 1152, 1155, 1195, 1223
BccI CCATC 6 cut(s) 191, 512, 544, 683, 800, 1066
BceAI ACGGC 2 cut(s) 571, 838
BciT130I CCWGG 3 cut(s) 484, 939, 1148
BcnI CCSGG 3 cut(s) 95, 285, 816
BcoDI GTCTC 1 cut(s) 558
BfaI CTAG 1 cut(s) 951
BfmI CTRYAG 1 cut(s) 1209
BfoI RGCGCY 1 cut(s) 189
BglII AGATCT 1 cut(s) 162
BisI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
BlpI GCTNAGC 1 cut(s) 49
BlsI GCNGC 7 cut(s) 55, 244, 506, 1142, 1145, 1210, 1213
BmcAI AGTACT 1 cut(s) 1028
Bme1390I CCNGG 6 cut(s) 95, 285, 484, 816, 939, 1148
Bme18I GGWCC 3 cut(s) 363, 947, 1199
BmeT110I CYCGRG 1 cut(s) 66
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
BmiI GGNNCC 8 cut(s) 187, 203, 321, 364, 814, 948, 1004, 1152
BmrFI CCNGG 6 cut(s) 95, 285, 484, 816, 939, 1148
BmrI ACTGGG 2 cut(s) 267, 1044
BmsI GCATC 5 cut(s) 199, 465, 702, 968, 1031
BmuI ACTGGG 2 cut(s) 267, 1044
BpiI GAAGAC 1 cut(s) 896
BpmI CTGGAG 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 49
BpuMI CCSGG 3 cut(s) 95, 285, 816
Bsa29I ATCGAT 1 cut(s) 1182
BsaAI YACGTR 1 cut(s) 1066
BsaBI GATNNNNATC 1 cut(s) 923
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 5 cut(s) 93, 115, 284, 814, 841
BsaWI WCCGGW 1 cut(s) 746
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 821, 944
Bse118I RCCGGY 2 cut(s) 74, 746
Bse1I ACTGG 6 cut(s) 262, 305, 330, 413, 1039, 1201
Bse3DI GCAATG 4 cut(s) 6, 457, 738, 786
Bse8I GATNNNNATC 1 cut(s) 923
BseBI CCWGG 3 cut(s) 484, 939, 1148
BseCI ATCGAT 1 cut(s) 1182
BseDI CCNNGG 5 cut(s) 93, 115, 284, 814, 841
BseGI GGATG 2 cut(s) 693, 1046
BseJI GATNNNNATC 1 cut(s) 923
BseLI CCNNNNNNNGG 3 cut(s) 78, 821, 944
BseMI GCAATG 4 cut(s) 6, 457, 738, 786
BseNI ACTGG 6 cut(s) 262, 305, 330, 413, 1039, 1201
BseXI GCAGC 6 cut(s) 40, 254, 1152, 1155, 1195, 1223
BsgI GTGCAG 2 cut(s) 1015, 1088
BshFI GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
BshNI GGYRCC 1 cut(s) 185
BshTI ACCGGT 1 cut(s) 746
BshVI ATCGAT 1 cut(s) 1182
BsiHKAI GWGCWC 1 cut(s) 858
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 5 cut(s) 75, 95, 284, 747, 816
BslFI GGGAC 3 cut(s) 301, 376, 1117
BslI CCNNNNNNNGG 3 cut(s) 78, 821, 944
BsmAI GTCTC 1 cut(s) 558
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 3 cut(s) 301, 376, 1117
BsnI GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 858
Bsp143I GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
Bsp1720I GCTNAGC 1 cut(s) 49
BspACI CCGC 1 cut(s) 504
BspANI GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
BspDI ATCGAT 1 cut(s) 1182
BspLI GGNNCC 8 cut(s) 187, 203, 321, 364, 814, 948, 1004, 1152
BspMAI CTGCAG 1 cut(s) 1213
BspPI GGATC 4 cut(s) 143, 271, 784, 949
BspQI GCTCTTC 1 cut(s) 393
BspT107I GGYRCC 1 cut(s) 185
BsrDI GCAATG 4 cut(s) 6, 457, 738, 786
BsrFI RCCGGY 2 cut(s) 74, 746
BsrI ACTGG 6 cut(s) 262, 305, 330, 413, 1039, 1201
BssAI RCCGGY 2 cut(s) 74, 746
BssECI CCNNGG 5 cut(s) 93, 115, 284, 814, 841
BssMI GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
BssNAI GTATAC 1 cut(s) 1194
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 841
Bst1107I GTATAC 1 cut(s) 1194
Bst2UI CCWGG 3 cut(s) 484, 939, 1148
Bst4CI ACNGT 2 cut(s) 883, 1113
Bst6I CTCTTC 1 cut(s) 393
BstACI GRCGYC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 1066
BstC8I GCNNGC 4 cut(s) 214, 240, 247, 1276
BstDEI CTNAG 1 cut(s) 49
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 2 cut(s) 693, 1046
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 7 cut(s) 151, 165, 279, 385, 779, 944, 1074
BstMAI GTCTC 1 cut(s) 558
BstMBI GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
BstMWI GCNNNNNNNGC 4 cut(s) 59, 68, 513, 522
BstNI CCWGG 3 cut(s) 484, 939, 1148
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 6 cut(s) 93, 283, 482, 814, 937, 1146
BstSFI CTRYAG 1 cut(s) 1209
BstV1I GCAGC 6 cut(s) 40, 254, 1152, 1155, 1195, 1223
BstV2I GAAGAC 1 cut(s) 896
BstX2I RGATCY 1 cut(s) 162
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 162
BstZ17I GTATAC 1 cut(s) 1194
Bsu15I ATCGAT 1 cut(s) 1182
BsuRI GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
BsuTUI ATCGAT 1 cut(s) 1182
BtgI CCRYGG 1 cut(s) 115
BtgZI GCGATG 3 cut(s) 531, 1044, 1092
BtrI CACGTC 1 cut(s) 868
BtsCI GGATG 2 cut(s) 693, 1046
BtsIMutI CAGTG 6 cut(s) 255, 312, 621, 684, 1032, 1279
Cac8I GCNNGC 4 cut(s) 214, 240, 247, 1276
CfoI GCGC 1 cut(s) 188
Cfr10I RCCGGY 2 cut(s) 74, 746
Cfr13I GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
ClaI ATCGAT 1 cut(s) 1182
Csp6I GTAC 1 cut(s) 1027
CspAI ACCGGT 1 cut(s) 746
CviAII CATG 9 cut(s) 137, 206, 217, 234, 246, 324, 766, 933, 988
CviQI GTAC 1 cut(s) 1027
DdeI CTNAG 1 cut(s) 49
DinI GGCGCC 1 cut(s) 187
DpnI GATC 7 cut(s) 150, 164, 278, 384, 778, 943, 1073
DpnII GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
DrdI GACNNNNNNGTC 1 cut(s) 1111
DseDI GACNNNNNNGTC 1 cut(s) 1111
EaeI YGGCCR 2 cut(s) 3, 935
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 841
Eco147I AGGCCT 1 cut(s) 375
Eco47I GGWCC 3 cut(s) 363, 947, 1199
Eco72I CACGTG 1 cut(s) 1066
Eco88I CYCGRG 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 947
EcoRII CCWGG 3 cut(s) 482, 937, 1146
EcoT14I CCWWGG 1 cut(s) 841
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 841
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 9 cut(s) 140, 209, 220, 237, 249, 327, 769, 936, 991
FaqI GGGAC 3 cut(s) 301, 376, 1117
FatI CATG 9 cut(s) 136, 205, 216, 233, 245, 323, 765, 932, 987
FblI GTMKAC 1 cut(s) 1193
Fnu4HI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
FokI GGATG 2 cut(s) 680, 1053
Fsp4HI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
FspBI CTAG 1 cut(s) 951
GlaI GCGC 1 cut(s) 187
GluI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
GsuI CTGGAG 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
HapII CCGG 5 cut(s) 75, 95, 284, 747, 816
HhaI GCGC 1 cut(s) 188
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 9 cut(s) 140, 209, 220, 237, 249, 327, 769, 936, 991
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HinfI GANTC 3 cut(s) 769, 876, 924
HpaII CCGG 5 cut(s) 75, 95, 284, 747, 816
HphI GGTGA 4 cut(s) 509, 761, 871, 1210
Hpy166II GTNNAC 3 cut(s) 530, 1084, 1194
Hpy188I TCNGA 1 cut(s) 276
Hpy188III TCNNGA 4 cut(s) 66, 152, 963, 1175
Hpy8I GTNNAC 3 cut(s) 530, 1084, 1194
HpyAV CCTTC 1 cut(s) 425
HpyCH4III ACNGT 2 cut(s) 883, 1113
HpyCH4IV ACGT 4 cut(s) 171, 867, 1065, 1224
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 68, 513, 522
HpyF3I CTNAG 1 cut(s) 49
HpySE526I ACGT 4 cut(s) 171, 867, 1065, 1224
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 9 cut(s) 140, 209, 220, 237, 249, 327, 769, 936, 991
HspAI GCGC 1 cut(s) 186
KasI GGCGCC 1 cut(s) 185
Kzo9I GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
LguI GCTCTTC 1 cut(s) 393
Lsp1109I GCAGC 6 cut(s) 40, 254, 1152, 1155, 1195, 1223
LweI GCATC 5 cut(s) 199, 465, 702, 968, 1031
MaeI CTAG 1 cut(s) 951
MaeII ACGT 4 cut(s) 171, 867, 1065, 1224
MaeIII GTNAC 2 cut(s) 497, 868
MalI GATC 7 cut(s) 150, 164, 278, 384, 778, 943, 1073
MboI GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
MboII GAAGA 4 cut(s) 172, 410, 898, 901
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 1 cut(s) 858
MlsI TGGCCA 2 cut(s) 5, 937
MluCI AATT 5 cut(s) 124, 220, 548, 804, 1014
MluNI TGGCCA 2 cut(s) 5, 937
Mly113I GGCGCC 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 735
MnlI CCTC 6 cut(s) 8, 73, 572, 602, 678, 1094
Mox20I TGGCCA 2 cut(s) 5, 937
MscI TGGCCA 2 cut(s) 5, 937
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 3 cut(s) 764, 861, 986
Msp20I TGGCCA 2 cut(s) 5, 937
MspI CCGG 5 cut(s) 75, 95, 284, 747, 816
MspR9I CCNGG 6 cut(s) 95, 285, 484, 816, 939, 1148
MvaI CCWGG 3 cut(s) 484, 939, 1148
MwoI GCNNNNNNNGC 4 cut(s) 59, 68, 513, 522
NarI GGCGCC 1 cut(s) 186
NciI CCSGG 3 cut(s) 95, 285, 816
NdeII GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
NlaIII CATG 9 cut(s) 140, 209, 220, 237, 249, 327, 769, 936, 991
NlaIV GGNNCC 8 cut(s) 187, 203, 321, 364, 814, 948, 1004, 1152
NmuCI GTSAC 2 cut(s) 497, 868
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PaeR7I CTCGAG 1 cut(s) 66
PceI AGGCCT 1 cut(s) 375
PciSI GCTCTTC 1 cut(s) 393
PcsI WCGNNNNNNNCGW 1 cut(s) 864
PfeI GAWTC 3 cut(s) 769, 876, 924
PflFI GACNNNGTC 1 cut(s) 569
PinAI ACCGGT 1 cut(s) 746
PkrI GCNGC 7 cut(s) 55, 244, 506, 1142, 1145, 1210, 1213
PluTI GGCGCC 1 cut(s) 189
PmaCI CACGTG 1 cut(s) 1066
PmlI CACGTG 1 cut(s) 1066
Ppu21I YACGTR 1 cut(s) 1066
PpuMI RGGWCCY 1 cut(s) 947
PsiI TTATAA 1 cut(s) 1013
Psp5II RGGWCCY 1 cut(s) 947
Psp6I CCWGG 3 cut(s) 482, 937, 1146
PspCI CACGTG 1 cut(s) 1066
PspGI CCWGG 3 cut(s) 482, 937, 1146
PspN4I GGNNCC 8 cut(s) 187, 203, 321, 364, 814, 948, 1004, 1152
PspPI GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
PspPPI RGGWCCY 1 cut(s) 947
PstI CTGCAG 1 cut(s) 1213
PsuI RGATCY 1 cut(s) 162
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 1 cut(s) 1028
RsaNI GTAC 1 cut(s) 1027
RseI CAYNNNNRTG 3 cut(s) 764, 861, 986
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
Sau3AI GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
Sau96I GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
ScaI AGTACT 1 cut(s) 1028
ScrFI CCNGG 6 cut(s) 95, 285, 484, 816, 939, 1148
SduI GDGCHC 1 cut(s) 858
SfaNI GCATC 5 cut(s) 199, 465, 702, 968, 1031
SfcI CTRYAG 1 cut(s) 1209
SfoI GGCGCC 1 cut(s) 187
Sfr274I CTCGAG 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 746
SinI GGWCC 3 cut(s) 363, 947, 1199
SlaI CTCGAG 1 cut(s) 66
SmiMI CAYNNNNRTG 3 cut(s) 764, 861, 986
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
SphI GCATGC 1 cut(s) 249
Sse9I AATT 5 cut(s) 124, 220, 548, 804, 1014
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 1 cut(s) 504
SspDI GGCGCC 1 cut(s) 185
SspMI CTAG 1 cut(s) 951
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 6 cut(s) 93, 283, 482, 814, 937, 1146
StyI CCWWGG 1 cut(s) 841
TaaI ACNGT 2 cut(s) 883, 1113
TaiI ACGT 4 cut(s) 174, 870, 1068, 1227
TaqI TCGA 5 cut(s) 67, 105, 577, 670, 1182
TasI AATT 5 cut(s) 124, 220, 548, 804, 1014
TatI WGTACW 1 cut(s) 1026
TauI GCSGC 1 cut(s) 507
TfiI GAWTC 3 cut(s) 769, 876, 924
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 5 cut(s) 262, 312, 628, 691, 1039
TseFI GTSAC 2 cut(s) 497, 868
TseI GCWGC 6 cut(s) 53, 242, 1140, 1143, 1208, 1211
Tsp45I GTSAC 2 cut(s) 497, 868
TspDTI ATGAA 6 cut(s) 214, 233, 411, 599, 607, 1123
TspGWI ACGGA 2 cut(s) 104, 480
TspRI CASTG 5 cut(s) 262, 312, 628, 691, 1039
Tth111I GACNNNGTC 1 cut(s) 569
VpaK11BI GGWCC 3 cut(s) 363, 947, 1199
XapI RAATTY 2 cut(s) 220, 548
XceI RCATGY 1 cut(s) 249
XhoI CTCGAG 1 cut(s) 66
XmiI GTMKAC 1 cut(s) 1193
XspI CTAG 1 cut(s) 951
ZrmI AGTACT 1 cut(s) 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.