RchiOBHm_Chr4g0423541

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008628
Physical Location & Seq
Forward (+)
49163370 .. 49164488
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39290

Sequence Viewer

Length: 750 bp
ATGCAGAACCTAAAATTTAACGGAGTCACAAACGGGCTCATCCGCGGCATCAATTCGATAAACCCTAAGGGAGTGCACATTTTCATCACGGACAGCCAGAACATTAGGGTGCGAGCAGTCTATATCGAAGCGCCCGGAACTAGCCCTAACACCGATGGCATACACATAAGTGACTCGAACAACGTGAGGATCGCCAGAACTCACATTTCTACCGGGGATGACTGCGTCGGTATGATTCAGGGATCCACCAACATTGCCATCAACAAAGTCTTTTGCGGACCTGGACATGGAATCAGCGTTGGTAGCCTTGGCAAATACGAGACTGAAAATGATGTGAGAGGGATCATTGTCAAGAACTGCACTTTTGCCAACACGGACAACGGTATCAGAATCAAGTCGTGGCCCGGATCGCAGCCAAGTCAAGCTACCGGCATGATTTTCCAGGACCTAATCATGCAAAATGTTAGGAACCCCATCATAATCGACCAGGAGTATGGTTGTAACGGCAAATGCAACAAAGCGCCATCGAAAGTGAAGATCAGTAATGTGCATTATATCAACGTTAAGGGAACCACATCATCGGATATTGCAGTGGACCTGATTTGCAGCAGCCAGTCTCCTTGTGAAAACATTGAGCTTCGTGACATTGATTTGAAGTATGTGGGTCCGAAGAAAGTTCGGTCTACCTCTCTGTGCAAGCATGCGAAAATCGGTTATGGTGGCATTCAAAACCCACCAGCTTGCCGTTGA

Protein Analysis

249

Amino Acids

26.9

Weight (kDa)

9.05

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 4 - 233 1.7e-69 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 683
AccII CGCG 1 cut(s) 45
AciI CCGC 3 cut(s) 43, 45, 276
AclI AACGTT 1 cut(s) 561
AclWI GGATC 5 cut(s) 197, 237, 250, 350, 415
AcsI RAATTY 1 cut(s) 14
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 2 cut(s) 655, 728
AjnI CCWGG 3 cut(s) 280, 441, 486
AleI CACNNNNGTG 1 cut(s) 168
AluBI AGCT 3 cut(s) 425, 637, 740
AluI AGCT 3 cut(s) 425, 637, 740
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 2 cut(s) 314, 621
Alw44I GTGCAC 1 cut(s) 74
AlwI GGATC 5 cut(s) 197, 237, 250, 350, 415
AoxI GGCC 1 cut(s) 401
ApaLI GTGCAC 1 cut(s) 74
ApeKI GCWGC 3 cut(s) 412, 606, 609
ApoI RAATTY 1 cut(s) 14
AspLEI GCGC 2 cut(s) 133, 523
AspS9I GGNCC 5 cut(s) 278, 402, 445, 595, 665
AsuC2I CCSGG 3 cut(s) 135, 214, 405
AvaII GGWCC 4 cut(s) 278, 445, 595, 665
AxyI CCTNAGG 1 cut(s) 66
BaeGI GKGCMC 1 cut(s) 78
BaeI ACNNNNGTAYC 2 cut(s) 367, 400
BamHI GGATCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 424, 618, 621
BccI CCATC 4 cut(s) 149, 266, 482, 532
BceAI ACGGC 2 cut(s) 520, 729
BciT130I CCWGG 3 cut(s) 282, 443, 488
BcnI CCSGG 3 cut(s) 135, 214, 405
BcoDI GTCTC 2 cut(s) 314, 621
BfaI CTAG 1 cut(s) 141
BfoI RGCGCY 2 cut(s) 134, 524
BisI GCNGC 4 cut(s) 46, 413, 607, 610
BlsI GCNGC 4 cut(s) 47, 414, 608, 611
Bme1390I CCNGG 6 cut(s) 135, 214, 282, 405, 443, 488
Bme18I GGWCC 4 cut(s) 278, 445, 595, 665
BmgT120I GGNCC 5 cut(s) 278, 402, 445, 595, 665
BmiI GGNNCC 4 cut(s) 244, 470, 571, 666
BmrFI CCNGG 6 cut(s) 135, 214, 282, 405, 443, 488
BmsI GCATC 1 cut(s) 57
BpuMI CCSGG 3 cut(s) 135, 214, 405
BsaJI CCNNGG 3 cut(s) 43, 213, 307
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse118I RCCGGY 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 613
Bse21I CCTNAGG 1 cut(s) 66
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 3 cut(s) 282, 443, 488
BseDI CCNNGG 3 cut(s) 43, 213, 307
BseGI GGATG 2 cut(s) 39, 223
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 613
BseSI GKGCMC 1 cut(s) 78
BseXI GCAGC 3 cut(s) 424, 618, 621
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 4 cut(s) 135, 213, 405, 429
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 2 cut(s) 314, 621
BsmI GAATGC 1 cut(s) 723
BsnI GGCC 1 cut(s) 403
Bsp1286I GDGCHC 2 cut(s) 39, 78
Bsp143I GATC 5 cut(s) 189, 242, 342, 407, 537
BspACI CCGC 3 cut(s) 43, 45, 276
BspANI GGCC 1 cut(s) 403
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 4 cut(s) 244, 470, 571, 666
BspPI GGATC 5 cut(s) 197, 237, 250, 350, 415
BsrDI GCAATG 1 cut(s) 252
BsrFI RCCGGY 1 cut(s) 428
BsrI ACTGG 1 cut(s) 613
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 3 cut(s) 43, 213, 307
BssMI GATC 5 cut(s) 189, 242, 342, 407, 537
BssT1I CCWWGG 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 282, 443, 488
Bst4CI ACNGT 1 cut(s) 383
BstC8I GCNNGC 4 cut(s) 114, 698, 702, 742
BstDEI CTNAG 1 cut(s) 66
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 2 cut(s) 39, 223
BstFNI CGCG 1 cut(s) 45
BstH2I RGCGCY 2 cut(s) 134, 524
BstHHI GCGC 2 cut(s) 133, 523
BstKTI GATC 5 cut(s) 192, 245, 345, 410, 540
BstMAI GTCTC 2 cut(s) 314, 621
BstMBI GATC 5 cut(s) 189, 242, 342, 407, 537
BstMWI GCNNNNNNNGC 2 cut(s) 303, 409
BstNI CCWGG 3 cut(s) 282, 443, 488
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 6 cut(s) 133, 212, 280, 403, 441, 486
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 3 cut(s) 424, 618, 621
BstX2I RGATCY 1 cut(s) 242
BstXI CCANNNNNNTGG 1 cut(s) 494
BstYI RGATCY 1 cut(s) 242
Bsu36I CCTNAGG 1 cut(s) 66
BsuRI GGCC 1 cut(s) 403
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 39, 223
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 1 cut(s) 597
Cac8I GCNNGC 4 cut(s) 114, 698, 702, 742
CfoI GCGC 2 cut(s) 133, 523
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 5 cut(s) 278, 402, 445, 595, 665
Cfr42I CCGCGG 1 cut(s) 46
CseI GACGC 1 cut(s) 214
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 4 cut(s) 287, 433, 454, 701
DdeI CTNAG 1 cut(s) 66
DpnI GATC 5 cut(s) 191, 244, 344, 409, 539
DpnII GATC 5 cut(s) 189, 242, 342, 407, 537
Eco130I CCWWGG 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 4 cut(s) 278, 445, 595, 665
Eco81I CCTNAGG 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 445
EcoRII CCWGG 3 cut(s) 280, 441, 486
EcoT14I CCWWGG 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 307
FaeI CATG 4 cut(s) 290, 436, 457, 704
FatI CATG 4 cut(s) 286, 432, 453, 700
FblI GTMKAC 1 cut(s) 683
Fnu4HI GCNGC 4 cut(s) 46, 413, 607, 610
FokI GGATG 2 cut(s) 26, 230
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 4 cut(s) 46, 413, 607, 610
FspBI CTAG 1 cut(s) 141
GlaI GCGC 2 cut(s) 132, 522
GluI GCNGC 4 cut(s) 46, 413, 607, 610
HaeII RGCGCY 2 cut(s) 134, 524
HaeIII GGCC 1 cut(s) 403
HapII CCGG 4 cut(s) 135, 213, 405, 429
HgaI GACGC 1 cut(s) 214
HhaI GCGC 2 cut(s) 133, 523
Hin1II CATG 4 cut(s) 290, 436, 457, 704
Hin6I GCGC 2 cut(s) 131, 521
HinP1I GCGC 2 cut(s) 131, 521
HinfI GANTC 5 cut(s) 24, 173, 235, 291, 390
HpaII CCGG 4 cut(s) 135, 213, 405, 429
Hpy166II GTNNAC 3 cut(s) 76, 595, 684
Hpy188I TCNGA 3 cut(s) 389, 583, 669
Hpy188III TCNNGA 2 cut(s) 352, 641
Hpy8I GTNNAC 3 cut(s) 76, 595, 684
Hpy99I CGWCG 1 cut(s) 230
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4IV ACGT 2 cut(s) 183, 561
HpyCH4V TGCA 9 cut(s) 4, 76, 360, 457, 513, 550, 590, 606, 696
HpyF10VI GCNNNNNNNGC 2 cut(s) 303, 409
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 2 cut(s) 183, 561
Hsp92II CATG 4 cut(s) 290, 436, 457, 704
HspAI GCGC 2 cut(s) 131, 521
KspI CCGCGG 1 cut(s) 46
Kzo9I GATC 5 cut(s) 189, 242, 342, 407, 537
Lsp1109I GCAGC 3 cut(s) 424, 618, 621
LweI GCATC 1 cut(s) 57
MaeI CTAG 1 cut(s) 141
MaeII ACGT 2 cut(s) 183, 561
MaeIII GTNAC 4 cut(s) 25, 170, 500, 641
MalI GATC 5 cut(s) 191, 244, 344, 409, 539
MboI GATC 5 cut(s) 189, 242, 342, 407, 537
MboII GAAGA 2 cut(s) 547, 682
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 2 cut(s) 39, 78
MluCI AATT 2 cut(s) 14, 52
MlyI GAGTC 2 cut(s) 33, 167
MnlI CCTC 3 cut(s) 180, 332, 697
MseI TTAA 2 cut(s) 18, 564
MslI CAYNNNNRTG 2 cut(s) 107, 168
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 4 cut(s) 135, 213, 405, 429
MspR9I CCNGG 6 cut(s) 135, 214, 282, 405, 443, 488
Mva1269I GAATGC 1 cut(s) 723
MvaI CCWGG 3 cut(s) 282, 443, 488
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 303, 409
NciI CCSGG 3 cut(s) 135, 214, 405
NdeII GATC 5 cut(s) 189, 242, 342, 407, 537
NlaIII CATG 4 cut(s) 290, 436, 457, 704
NlaIV GGNNCC 4 cut(s) 244, 470, 571, 666
NmuCI GTSAC 3 cut(s) 25, 170, 641
NspI RCATGY 1 cut(s) 704
OliI CACNNNNGTG 1 cut(s) 168
PaeI GCATGC 1 cut(s) 704
PctI GAATGC 1 cut(s) 723
PfeI GAWTC 3 cut(s) 235, 291, 390
PflFI GACNNNGTC 1 cut(s) 224
PfoI TCCNGGA 1 cut(s) 441
PkrI GCNGC 4 cut(s) 47, 414, 608, 611
PleI GAGTC 2 cut(s) 32, 167
PpsI GAGTC 2 cut(s) 32, 167
PpuMI RGGWCCY 1 cut(s) 445
Psp1406I AACGTT 1 cut(s) 561
Psp5II RGGWCCY 1 cut(s) 445
Psp6I CCWGG 3 cut(s) 280, 441, 486
PspGI CCWGG 3 cut(s) 280, 441, 486
PspN4I GGNNCC 4 cut(s) 244, 470, 571, 666
PspPI GGNCC 5 cut(s) 278, 402, 445, 595, 665
PspPPI RGGWCCY 1 cut(s) 445
PsuI RGATCY 1 cut(s) 242
PsyI GACNNNGTC 1 cut(s) 224
RseI CAYNNNNRTG 2 cut(s) 107, 168
SacII CCGCGG 1 cut(s) 46
SaqAI TTAA 2 cut(s) 18, 564
SatI GCNGC 4 cut(s) 46, 413, 607, 610
Sau3AI GATC 5 cut(s) 189, 242, 342, 407, 537
Sau96I GGNCC 5 cut(s) 278, 402, 445, 595, 665
SchI GAGTC 2 cut(s) 33, 167
ScrFI CCNGG 6 cut(s) 135, 214, 282, 405, 443, 488
SduI GDGCHC 2 cut(s) 39, 78
SfaNI GCATC 1 cut(s) 57
Sfr303I CCGCGG 1 cut(s) 46
SgrBI CCGCGG 1 cut(s) 46
SinI GGWCC 4 cut(s) 278, 445, 595, 665
SmiMI CAYNNNNRTG 2 cut(s) 107, 168
SphI GCATGC 1 cut(s) 704
Sse9I AATT 2 cut(s) 14, 52
SsiI CCGC 3 cut(s) 43, 45, 276
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 6 cut(s) 133, 212, 280, 403, 441, 486
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 1 cut(s) 383
TaiI ACGT 2 cut(s) 186, 564
TaqI TCGA 5 cut(s) 56, 126, 176, 483, 527
TaqII GACCGA 1 cut(s) 669
TasI AATT 2 cut(s) 14, 52
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 3 cut(s) 235, 291, 390
Tru1I TTAA 2 cut(s) 18, 564
Tru9I TTAA 2 cut(s) 18, 564
TscAI CASTG 1 cut(s) 597
TseFI GTSAC 3 cut(s) 25, 170, 641
TseI GCWGC 3 cut(s) 412, 606, 609
Tsp45I GTSAC 3 cut(s) 25, 170, 641
TspDTI ATGAA 1 cut(s) 73
TspGWI ACGGA 3 cut(s) 36, 104, 389
TspRI CASTG 1 cut(s) 597
Tth111I GACNNNGTC 1 cut(s) 224
VneI GTGCAC 1 cut(s) 74
VpaK11BI GGWCC 4 cut(s) 278, 445, 595, 665
XapI RAATTY 1 cut(s) 14
XceI RCATGY 1 cut(s) 704
XmiI GTMKAC 1 cut(s) 683
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.