RchiOBHm_Chr4g0423531

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008628
Physical Location & Seq
Forward (+)
49162549 .. 49163318
770 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39289

Sequence Viewer

Length: 585 bp
ATGGCTATCAATGCAGGAATGTCTTGGCGGAGCCACGCCTTTCTTATCGTGTGCTTAGTTCCACTAGCCCATCTTGCGACCGCCCGTGAAAGACAGATAATTCCTCCGGTATTACCCGATGTTTTCCGCGGCAACAATTTGGCTCGCCATGGTAGAGCTGATCCCACTGAGAAAATCTTCAACGTTCTAGATTTTGGCGCCGTGGCCGATGGAAAGAAAGATTCAGCCATTTATTTCATACGAACATGGATTGCGGCGTGCCGGGGTACTCACACTGGGAAATCAAGGGTTGTGATTCCGGAAGGGACGTTCAAAGCAGGGCCAGTTATATTTCAGGGTCCATGCACCTGCGCGAAACCTATAATTGTTGAAATTAAAGGGACCGTATTAGGAGCCAATGACTTATCCTTATATGAAGAACCATACTGGATTTTATTTGAAAAGGTGGACGGAGTGATAGTTACAGGCAAGGGTACTCTTAATGGCCAAGGTTCAAGTGTCTGGAAAAACGTTGAGAGCTGCAGCGGTAGCGACTGCAGTCCACTCCCATCGGTAGGTATTGTTGAGAATTCTAAGCATTGCTAG

Protein Analysis

194

Amino Acids

20.89

Weight (kDa)

8.48

Isoelectric Point (pI)

24.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 91 - 185 1.5e-09 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 197
AccII CGCG 2 cut(s) 129, 353
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 6 cut(s) 28, 81, 127, 129, 254, 525
AclI AACGTT 2 cut(s) 183, 510
AclWI GGATC 1 cut(s) 155
AcoI YGGCCR 2 cut(s) 204, 484
AcsI RAATTY 1 cut(s) 568
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 2 cut(s) 268, 475
AfiI CCNNNNNNNGG 1 cut(s) 554
AgsI TTSAA 5 cut(s) 181, 313, 371, 440, 495
AloI GAACNNNNNNTCC 2 cut(s) 293, 325
AluBI AGCT 2 cut(s) 158, 519
AluI AGCT 2 cut(s) 158, 519
AlwI GGATC 1 cut(s) 155
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 3 cut(s) 204, 320, 484
ApeKI GCWGC 2 cut(s) 519, 522
ApoI RAATTY 1 cut(s) 568
Asp700I GAANNNNTTC 1 cut(s) 176
AspLEI GCGC 2 cut(s) 200, 353
AspS9I GGNCC 3 cut(s) 320, 338, 381
AsuC2I CCSGG 1 cut(s) 263
AvaII GGWCC 2 cut(s) 338, 381
BalI TGGCCA 1 cut(s) 486
BanI GGYRCC 1 cut(s) 197
BbvI GCAGC 2 cut(s) 506, 534
BccI CCATC 3 cut(s) 78, 203, 556
BceAI ACGGC 1 cut(s) 185
BcnI CCSGG 1 cut(s) 263
BfaI CTAG 3 cut(s) 65, 188, 583
BfmI CTRYAG 2 cut(s) 520, 535
BfoI RGCGCY 1 cut(s) 201
BfuAI ACCTGC 1 cut(s) 356
BisI GCNGC 4 cut(s) 130, 255, 520, 523
BlsI GCNGC 4 cut(s) 131, 256, 521, 524
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 2 cut(s) 338, 381
BmgT120I GGNCC 3 cut(s) 320, 338, 381
BmiI GGNNCC 5 cut(s) 32, 199, 339, 382, 394
BmrFI CCNGG 1 cut(s) 263
BmrI ACTGGG 1 cut(s) 285
BmuI ACTGGG 1 cut(s) 285
BoxI GACNNNNGTC 1 cut(s) 537
BpuMI CCSGG 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 5 cut(s) 127, 148, 201, 262, 487
BsaWI WCCGGW 2 cut(s) 106, 298
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 554
Bse1I ACTGG 3 cut(s) 280, 323, 431
Bse3DI GCAATG 1 cut(s) 577
BseAI TCCGGA 1 cut(s) 298
BseDI CCNNGG 5 cut(s) 127, 148, 201, 262, 487
BseLI CCNNNNNNNGG 1 cut(s) 554
BseMI GCAATG 1 cut(s) 577
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 3 cut(s) 280, 323, 431
BseXI GCAGC 2 cut(s) 506, 534
Bsh1236I CGCG 2 cut(s) 129, 353
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 3 cut(s) 206, 322, 486
BshNI GGYRCC 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 81
BsiSI CCGG 3 cut(s) 107, 262, 299
BslFI GGGAC 2 cut(s) 319, 394
BslI CCNNNNNNNGG 1 cut(s) 554
BsmFI GGGAC 2 cut(s) 319, 394
BsnI GGCC 3 cut(s) 206, 322, 486
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 1 cut(s) 160
Bsp19I CCATGG 1 cut(s) 148
BspACI CCGC 6 cut(s) 28, 81, 127, 129, 254, 525
BspANI GGCC 3 cut(s) 206, 322, 486
BspCNI CTCAG 1 cut(s) 160
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 2 cut(s) 129, 353
BspLI GGNNCC 5 cut(s) 32, 199, 339, 382, 394
BspMAI CTGCAG 2 cut(s) 524, 539
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 577
BsrI ACTGG 3 cut(s) 280, 323, 431
BssECI CCNNGG 5 cut(s) 127, 148, 201, 262, 487
BssMI GATC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 148, 487
Bst4CI ACNGT 1 cut(s) 385
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 145, 259
BstDEI CTNAG 3 cut(s) 55, 168, 573
BstDSI CCRYGG 3 cut(s) 127, 148, 201
BstFNI CGCG 2 cut(s) 129, 353
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 2 cut(s) 200, 353
BstKTI GATC 1 cut(s) 163
BstMBI GATC 1 cut(s) 160
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 3 cut(s) 11, 74, 528
BstPAI GACNNNNGTC 1 cut(s) 537
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 2 cut(s) 520, 535
BstUI CGCG 2 cut(s) 129, 353
BstV1I GCAGC 2 cut(s) 506, 534
BsuRI GGCC 3 cut(s) 206, 322, 486
BtgI CCRYGG 3 cut(s) 127, 148, 201
BtsIMutI CAGTG 2 cut(s) 165, 273
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 2 cut(s) 145, 259
CfoI GCGC 2 cut(s) 200, 353
Cfr13I GGNCC 3 cut(s) 320, 338, 381
Cfr42I CCGCGG 1 cut(s) 130
Csp6I GTAC 2 cut(s) 267, 474
CviAII CATG 3 cut(s) 149, 246, 342
CviQI GTAC 2 cut(s) 267, 474
DdeI CTNAG 3 cut(s) 55, 168, 573
DinI GGCGCC 1 cut(s) 199
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
EaeI YGGCCR 2 cut(s) 204, 484
EciI GGCGGA 1 cut(s) 43
Eco130I CCWWGG 2 cut(s) 148, 487
Eco47I GGWCC 2 cut(s) 338, 381
EcoRI GAATTC 1 cut(s) 568
EcoT14I CCWWGG 2 cut(s) 148, 487
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 2 cut(s) 148, 487
FaeI CATG 3 cut(s) 152, 249, 345
FaiI YATR 9 cut(s) 150, 239, 247, 329, 343, 362, 412, 414, 424
FaqI GGGAC 2 cut(s) 319, 394
FatI CATG 3 cut(s) 148, 245, 341
Fnu4HI GCNGC 4 cut(s) 130, 255, 520, 523
Fsp4HI GCNGC 4 cut(s) 130, 255, 520, 523
FspBI CTAG 3 cut(s) 65, 188, 583
GlaI GCGC 2 cut(s) 199, 352
GluI GCNGC 4 cut(s) 130, 255, 520, 523
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 3 cut(s) 206, 322, 486
HapII CCGG 3 cut(s) 107, 262, 299
HhaI GCGC 2 cut(s) 200, 353
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 3 cut(s) 152, 249, 345
Hin6I GCGC 2 cut(s) 198, 351
HinP1I GCGC 2 cut(s) 198, 351
HinfI GANTC 2 cut(s) 221, 295
HpaII CCGG 3 cut(s) 107, 262, 299
Hpy166II GTNNAC 2 cut(s) 448, 542
Hpy188III TCNNGA 3 cut(s) 188, 299, 502
Hpy8I GTNNAC 2 cut(s) 448, 542
HpyAV CCTTC 1 cut(s) 296
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 3 cut(s) 183, 308, 510
HpyCH4V TGCA 4 cut(s) 14, 345, 522, 537
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 74, 528
HpyF3I CTNAG 3 cut(s) 55, 168, 573
HpySE526I ACGT 3 cut(s) 183, 308, 510
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 3 cut(s) 152, 249, 345
HspAI GCGC 2 cut(s) 198, 351
KasI GGCGCC 1 cut(s) 197
Kpn2I TCCGGA 1 cut(s) 298
KspI CCGCGG 1 cut(s) 130
Kzo9I GATC 1 cut(s) 160
LmnI GCTCC 2 cut(s) 30, 392
Lsp1109I GCAGC 2 cut(s) 506, 534
MaeI CTAG 3 cut(s) 65, 188, 583
MaeII ACGT 3 cut(s) 183, 308, 510
MaeIII GTNAC 1 cut(s) 460
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MboII GAAGA 2 cut(s) 169, 428
MlsI TGGCCA 1 cut(s) 486
MluCI AATT 5 cut(s) 99, 136, 363, 372, 568
MluNI TGGCCA 1 cut(s) 486
Mly113I GGCGCC 1 cut(s) 198
MnlI CCTC 1 cut(s) 114
Mox20I TGGCCA 1 cut(s) 486
MroI TCCGGA 1 cut(s) 298
MroXI GAANNNNTTC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 486
MseI TTAA 2 cut(s) 375, 480
Msp20I TGGCCA 1 cut(s) 486
MspA1I CMGCKG 2 cut(s) 129, 525
MspI CCGG 3 cut(s) 107, 262, 299
MspR9I CCNGG 1 cut(s) 263
MvnI CGCG 2 cut(s) 129, 353
MwoI GCNNNNNNNGC 3 cut(s) 11, 74, 528
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 1 cut(s) 263
NcoI CCATGG 1 cut(s) 148
NdeII GATC 1 cut(s) 160
NlaIII CATG 3 cut(s) 152, 249, 345
NlaIV GGNNCC 5 cut(s) 32, 199, 339, 382, 394
PaqCI CACCTGC 1 cut(s) 356
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 221, 295
PkrI GCNGC 4 cut(s) 131, 256, 521, 524
PluTI GGCGCC 1 cut(s) 201
PshAI GACNNNNGTC 1 cut(s) 537
Psp1406I AACGTT 2 cut(s) 183, 510
PspN4I GGNNCC 5 cut(s) 32, 199, 339, 382, 394
PspPI GGNCC 3 cut(s) 320, 338, 381
PstI CTGCAG 2 cut(s) 524, 539
RsaI GTAC 2 cut(s) 268, 475
RsaNI GTAC 2 cut(s) 267, 474
SacII CCGCGG 1 cut(s) 130
SaqAI TTAA 2 cut(s) 375, 480
SatI GCNGC 4 cut(s) 130, 255, 520, 523
Sau3AI GATC 1 cut(s) 160
Sau96I GGNCC 3 cut(s) 320, 338, 381
ScrFI CCNGG 1 cut(s) 263
SfcI CTRYAG 2 cut(s) 520, 535
SfoI GGCGCC 1 cut(s) 199
Sfr303I CCGCGG 1 cut(s) 130
SgrBI CCGCGG 1 cut(s) 130
SinI GGWCC 2 cut(s) 338, 381
Sse9I AATT 5 cut(s) 99, 136, 363, 372, 568
SsiI CCGC 6 cut(s) 28, 81, 127, 129, 254, 525
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 3 cut(s) 65, 188, 583
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 2 cut(s) 148, 487
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 3 cut(s) 186, 311, 513
TasI AATT 5 cut(s) 99, 136, 363, 372, 568
TauI GCSGC 2 cut(s) 132, 257
TfiI GAWTC 2 cut(s) 221, 295
Tru1I TTAA 2 cut(s) 375, 480
Tru9I TTAA 2 cut(s) 375, 480
TscAI CASTG 2 cut(s) 172, 280
TseI GCWGC 2 cut(s) 519, 522
TspDTI ATGAA 2 cut(s) 226, 429
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 2 cut(s) 172, 280
VpaK11BI GGWCC 2 cut(s) 338, 381
XapI RAATTY 1 cut(s) 568
XbaI TCTAGA 1 cut(s) 187
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 3 cut(s) 65, 188, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.