Rw4G025220

receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016827
Physical Location & Seq
Forward (+)
51254451 .. 51256989
2539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025220.1

Sequence Viewer

Length: 705 bp
ATGAATTTCTATACATGGCATCCTCATTTAAAACAAAAAAAAATGGTTTCTCGAGTTCTGCTTTGTTTTCTGTTCGCAGTTGCCACTGCATCTGTGGATTGTAACTTGGAAGGGGATATTCTTTATTCCTGGAAAACCATGTTGGTCGACCCTAACAATGTTCTTGCTAGCTGGAACCTGACATTACCCAATCCATGCTCTTGGTTCCATGTTACCTGCAATAACGAAAACATCGTTACAAGGCTGGACCTTGGCAATGCTGGATTATCAGGACCTATTATCCCTGCGCTTGCAAATTTGACTAACCTCCAATACTTGGAGCTATACCAGAACAAATTTAATGGATCTATTCCATCGGAACTTGGTCACTTAAAAGACCTGGTCAGCTTGGACTTGTACTGGAACCAACTCTCGGGTTCAATTCCAGAGACATTAGGTCACTTGAATTCATTGAGATTTTTGAGGGTCTTTCGCAATAATCTGACTGGACCTATTCCTTGGTCTCTTGGCAATTTGACAAGTTTACAAATCCTGAAATTAAACAAGAACCGTCTAACTGGTGCTTTACCAGTAGAGGTCATTGAACTCGTTCGTTTTGGCAACTTGGGCGTTTTGGATGTCTCAAACAATCTGTTGGCAGGAACCGTACACCCCACCAATTCAACAGTTACAAAAATCATACAAGATCCGAAAGCACGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

234

Amino Acids

26.1

Weight (kDa)

8.69

Isoelectric Point (pI)

23.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 74 5.5e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 91 - 208 3e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016827)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23091 FvH4_4g23092 FvH4_4g23092
malus_domestica MD13G1125600.v1.1
pyrus_communis pycom13g10960
rosa_chinensis RchiOBHm_Chr4g0429381
rosa_laevigata RLG00000007075
rosa_multiflora Rmu_sc0003708.1_g000022
rosa_roxburghii Rroxscaffold_5G00371020
rosa_rugosa Rorug04G0235400
rosa_samantha Rh4AG291300 Rh4CG312600 Rh4DG294100
rosa_wichuraiana Rw4G025220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 316
AccI GTMKAC 1 cut(s) 147
AclWI GGATC 2 cut(s) 352, 680
AcsI RAATTY 4 cut(s) 4, 295, 335, 445
AfaI GTAC 2 cut(s) 398, 648
AfiI CCNNNNNNNGG 2 cut(s) 316, 412
AgsI TTSAA 4 cut(s) 420, 445, 584, 663
AhdI GACNNNNNGTC 1 cut(s) 435
AjnI CCWGG 2 cut(s) 128, 378
AjuI GAANNNNNNNTTGG 2 cut(s) 125, 157
AluBI AGCT 3 cut(s) 171, 322, 387
AluI AGCT 3 cut(s) 171, 322, 387
Alw26I GTCTC 3 cut(s) 422, 507, 625
AlwI GGATC 2 cut(s) 352, 680
Ama87I CYCGRG 2 cut(s) 51, 412
ApoI RAATTY 4 cut(s) 4, 295, 335, 445
Asp700I GAANNNNTTC 1 cut(s) 588
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 3 cut(s) 247, 272, 488
AsuNHI GCTAGC 1 cut(s) 167
AvaI CYCGRG 2 cut(s) 51, 412
AvaII GGWCC 3 cut(s) 247, 272, 488
BccI CCATC 1 cut(s) 361
BciT130I CCWGG 2 cut(s) 130, 380
BcoDI GTCTC 3 cut(s) 422, 507, 625
BfaI CTAG 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 130, 380
Bme18I GGWCC 3 cut(s) 247, 272, 488
BmeRI GACNNNNNGTC 1 cut(s) 435
BmeT110I CYCGRG 2 cut(s) 51, 412
BmgT120I GGNCC 3 cut(s) 247, 272, 488
BmiI GGNNCC 4 cut(s) 176, 206, 404, 643
BmrFI CCNGG 2 cut(s) 130, 380
BmsI GCATC 2 cut(s) 28, 98
BmtI GCTAGC 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 698
BsaI GGTCTC 1 cut(s) 507
BsaJI CCNNGG 2 cut(s) 250, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 412
Bse1I ACTGG 4 cut(s) 404, 490, 562, 569
Bse3DI GCAATG 1 cut(s) 262
BseBI CCWGG 2 cut(s) 130, 380
BseDI CCNNGG 2 cut(s) 250, 497
BseGI GGATG 2 cut(s) 19, 622
BseLI CCNNNNNNNGG 2 cut(s) 316, 412
BseMI GCAATG 1 cut(s) 262
BseNI ACTGG 4 cut(s) 404, 490, 562, 569
BsiHKCI CYCGRG 2 cut(s) 51, 412
BslI CCNNNNNNNGG 2 cut(s) 316, 412
BsmAI GTCTC 3 cut(s) 422, 507, 625
Bso31I GGTCTC 1 cut(s) 507
BsoBI CYCGRG 2 cut(s) 51, 412
Bsp143I GATC 2 cut(s) 344, 685
BspLI GGNNCC 4 cut(s) 176, 206, 404, 643
BspMI ACCTGC 1 cut(s) 224
BspOI GCTAGC 1 cut(s) 171
BspPI GGATC 2 cut(s) 352, 680
BspTNI GGTCTC 1 cut(s) 507
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 4 cut(s) 404, 490, 562, 569
BssECI CCNNGG 2 cut(s) 250, 497
BssMI GATC 2 cut(s) 344, 685
BssT1I CCWWGG 2 cut(s) 250, 497
Bst2UI CCWGG 2 cut(s) 130, 380
Bst4CI ACNGT 3 cut(s) 551, 646, 667
BstBAI YACGTR 1 cut(s) 698
BstC8I GCNNGC 2 cut(s) 169, 291
BstF5I GGATG 2 cut(s) 19, 622
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 2 cut(s) 347, 688
BstMAI GTCTC 3 cut(s) 422, 507, 625
BstMBI GATC 2 cut(s) 344, 685
BstMWI GCNNNNNNNGC 1 cut(s) 606
BstNI CCWGG 2 cut(s) 130, 380
BstSCI CCNGG 2 cut(s) 128, 378
BstX2I RGATCY 2 cut(s) 344, 685
BstXI CCANNNNNNTGG 1 cut(s) 201
BstYI RGATCY 2 cut(s) 344, 685
BtsCI GGATG 2 cut(s) 19, 622
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 84
BveI ACCTGC 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 169, 291
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 3 cut(s) 247, 272, 488
CsiI ACCWGGT 1 cut(s) 378
Csp6I GTAC 2 cut(s) 397, 647
CviAII CATG 4 cut(s) 15, 139, 195, 209
CviJI RGCY 4 cut(s) 171, 244, 322, 387
CviKI_1 RGCY 4 cut(s) 171, 244, 322, 387
CviQI GTAC 2 cut(s) 397, 647
DpnI GATC 2 cut(s) 346, 687
DpnII GATC 2 cut(s) 344, 685
DraI TTTAAA 1 cut(s) 30
DriI GACNNNNNGTC 1 cut(s) 435
Eam1105I GACNNNNNGTC 1 cut(s) 435
Eco130I CCWWGG 2 cut(s) 250, 497
Eco31I GGTCTC 1 cut(s) 507
Eco47I GGWCC 3 cut(s) 247, 272, 488
Eco88I CYCGRG 2 cut(s) 51, 412
EcoO109I RGGNCCY 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 445
EcoRII CCWGG 2 cut(s) 128, 378
EcoT14I CCWWGG 2 cut(s) 250, 497
ErhI CCWWGG 2 cut(s) 250, 497
FaeI CATG 4 cut(s) 18, 142, 198, 212
FaiI YATR 7 cut(s) 12, 16, 140, 196, 210, 325, 680
FatI CATG 4 cut(s) 14, 138, 194, 208
FblI GTMKAC 1 cut(s) 147
FokI GGATG 2 cut(s) 6, 629
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 288
HhaI GCGC 1 cut(s) 289
Hin1II CATG 4 cut(s) 18, 142, 198, 212
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
Hpy166II GTNNAC 3 cut(s) 148, 524, 649
Hpy188I TCNGA 3 cut(s) 358, 483, 690
Hpy188III TCNNGA 4 cut(s) 51, 270, 425, 532
Hpy8I GTNNAC 3 cut(s) 148, 524, 649
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 3 cut(s) 551, 646, 667
HpyCH4IV ACGT 1 cut(s) 697
HpyCH4V TGCA 3 cut(s) 89, 219, 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 606
HpySE526I ACGT 1 cut(s) 697
Hsp92II CATG 4 cut(s) 18, 142, 198, 212
HspAI GCGC 1 cut(s) 287
Kzo9I GATC 2 cut(s) 344, 685
LmnI GCTCC 1 cut(s) 319
LweI GCATC 2 cut(s) 28, 98
MabI ACCWGGT 1 cut(s) 378
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 697
MaeIII GTNAC 7 cut(s) 101, 211, 235, 365, 437, 667, 698
MalI GATC 2 cut(s) 346, 687
MboI GATC 2 cut(s) 344, 685
MflI RGATCY 2 cut(s) 344, 685
MluCI AATT 8 cut(s) 4, 295, 335, 420, 445, 511, 536, 658
MnlI CCTC 4 cut(s) 33, 317, 456, 568
MroXI GAANNNNTTC 1 cut(s) 588
MseI TTAA 4 cut(s) 29, 339, 371, 539
MspR9I CCNGG 2 cut(s) 130, 380
MvaI CCWGG 2 cut(s) 130, 380
MwoI GCNNNNNNNGC 1 cut(s) 606
NdeII GATC 2 cut(s) 344, 685
NheI GCTAGC 1 cut(s) 167
NlaIII CATG 4 cut(s) 18, 142, 198, 212
NlaIV GGNNCC 4 cut(s) 176, 206, 404, 643
NmuCI GTSAC 2 cut(s) 365, 437
PaeR7I CTCGAG 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 588
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 316
PfoI TCCNGGA 1 cut(s) 128
Ppu21I YACGTR 1 cut(s) 698
PpuMI RGGWCCY 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 2 cut(s) 128, 378
PspGI CCWGG 2 cut(s) 128, 378
PspN4I GGNNCC 4 cut(s) 176, 206, 404, 643
PspPI GGNCC 3 cut(s) 247, 272, 488
PspPPI RGGWCCY 1 cut(s) 272
PsuI RGATCY 2 cut(s) 344, 685
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 2 cut(s) 398, 648
RsaNI GTAC 2 cut(s) 397, 647
SalI GTCGAC 1 cut(s) 146
SaqAI TTAA 4 cut(s) 29, 339, 371, 539
Sau3AI GATC 2 cut(s) 344, 685
Sau96I GGNCC 3 cut(s) 247, 272, 488
ScrFI CCNGG 2 cut(s) 130, 380
SexAI ACCWGGT 1 cut(s) 378
SfaNI GCATC 2 cut(s) 28, 98
Sfr274I CTCGAG 1 cut(s) 51
SinI GGWCC 3 cut(s) 247, 272, 488
SlaI CTCGAG 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 51
SmoI CTYRAG 1 cut(s) 51
Sse9I AATT 8 cut(s) 4, 295, 335, 420, 445, 511, 536, 658
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 128, 378
StyI CCWWGG 2 cut(s) 250, 497
TaaI ACNGT 3 cut(s) 551, 646, 667
TaiI ACGT 1 cut(s) 700
TaqI TCGA 2 cut(s) 52, 147
TasI AATT 8 cut(s) 4, 295, 335, 420, 445, 511, 536, 658
TatI WGTACW 1 cut(s) 396
Tru1I TTAA 4 cut(s) 29, 339, 371, 539
Tru9I TTAA 4 cut(s) 29, 339, 371, 539
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 2 cut(s) 365, 437
Tsp45I GTSAC 2 cut(s) 365, 437
TspDTI ATGAA 2 cut(s) 17, 438
TspRI CASTG 1 cut(s) 91
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 3 cut(s) 247, 272, 488
XapI RAATTY 4 cut(s) 4, 295, 335, 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 91
XhoI CTCGAG 1 cut(s) 51
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 588
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.