Rw4G033080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016107
Physical Location & Seq
Reverse (-)
60209917 .. 60211011
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033080.1

Sequence Viewer

Length: 1095 bp
ATGACTTGTTCAATCGCCTCCTCCGTCCTTGTTGTTTTCATTTTGCTGAGCTCAATCCCGATCCAAACCCAAGCAAGCAAGCCTCACGTCATCAATTTCCGATCCCCAAATCTCTACCCCGAAGGCCTAGCCTACGACCCCTCCGCCCAGCACTTCATCGTCGGGGCCATCCGCCAACGCACCATCTACTCCGTCTCCGACGCCGGCGTAACCGACCTTCTCATCTCCGACCCCACTCTCCCCGAAAACGTCACCTTCCTCGGCCTCGCCGTCGACACCGTCAACAACCGCCTCCTCGCCGCCGTCCAAGCCGCGGAGCCGCTCCCTCACTTCAGCGCCCTCGCCGCCTACGACCTCCGCACGCGCCGCCGCCTCTTCCTCACGCCCCTCCCCTCCGCCGAAGGCGACGCCTCCCGCCAGATCGCCAACGACGTCGCCGTGGATTTCAAGGGCAACGCCTACGTCACCAACTCGGCGGCGAACTTCATCTGGAAAGTCAACTCCGACGGCGCGGCCTCCGTCCTCTCCAAGTCCCCGCTATTCTCGGCGCATCCTGTGGACCCCGACTGGCCCTATAGCTTCTGCGGCCTCAACGGCCTTGCTTACGTCAGCAAGGGGTATCTACTGGTGGTGCAATCCAACACCGGCAAGCTGTTCAAGGTGGACGCGGAGGACGGAGCCGCCAGGGTGGTGCTGCTGCCGGAGGACTTGCCTCTGGCCGACGGGATTGCTGTCCGACGAGACGGGACCGTTGTGGTGGTTTCCATGAATAAAATGTGGTTTCTTAAGAGCCAAGACAGCTGGGGAGAGGGTGTGGTTTATGACAAGACGGAGCTTGAAGTGGAGAGGTTTCCGACCTCGGTGGCCGTGGCAGCGGAGGACAGGGTGTACGTGATATACGGGCATGCGCTTGAGGGAATGACGGGGAATTCGGAGAGGGAGATGTTTAGTATAGCGGAAGTGAGGTCGGAGAAGGAGAGCAAGGAAGAGGGTGTTTGGATTTTTGTTTTGGTTGGGTTGGGTCTGGCTTATTTCATGTTCTGGAGGTTTCAGATGCGCCATCTTGTTGGCAGCATGAACAAAAAGACCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SGL

Protein Analysis

364

Amino Acids

39.92

Weight (kDa)

5.46

Isoelectric Point (pI)

27.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01410
fragaria_vesca FvH4_4g32390
malus_domestica MD16G1028500.v1.1
prunus_persica Prupe.1G324200_v2.0.a1
pyrus_communis pycom16g02440
rosa_laevigata RLG00000006110
rosa_multiflora Rmu_co8419081.1_g000001
rosa_roxburghii Rroxscaffold_5G00382130
rosa_rugosa Rorug04G0331600 Rorug07G0286600
rosa_samantha Rh4AG383400 Rh4BG397500 Rh4CG412700 Rh4DG390200
rosa_wichuraiana Rw4G033080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 435
AccBSI CCGCTC 1 cut(s) 322
AccI GTMKAC 1 cut(s) 273
AccII CGCG 4 cut(s) 314, 364, 512, 668
AclWI GGATC 2 cut(s) 55, 96
AcoI YGGCCR 2 cut(s) 717, 864
AcsI RAATTY 1 cut(s) 928
AcuI CTGAAG 1 cut(s) 316
AcyI GRCGYC 3 cut(s) 201, 408, 432
AfaI GTAC 1 cut(s) 890
AfiI CCNNNNNNNGG 1 cut(s) 313
AflII CTTAAG 1 cut(s) 785
AgsI TTSAA 4 cut(s) 12, 448, 658, 839
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 1 cut(s) 683
AluBI AGCT 5 cut(s) 51, 579, 652, 801, 835
AluI AGCT 5 cut(s) 51, 579, 652, 801, 835
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 2 cut(s) 199, 735
AlwI GGATC 2 cut(s) 55, 96
AoxI GGCC 9 cut(s) 124, 165, 262, 513, 569, 586, 595, 717, 864
ApeKI GCWGC 4 cut(s) 694, 697, 872, 1071
ApoI RAATTY 1 cut(s) 928
ArsI GACNNNNNNTTYG 2 cut(s) 913, 945
AspLEI GCGC 6 cut(s) 338, 366, 512, 550, 910, 1059
AspS9I GGNCC 4 cut(s) 165, 559, 570, 747
AsuHPI GGTGA 2 cut(s) 244, 457
AvaII GGWCC 2 cut(s) 559, 747
BanII GRGCYC 1 cut(s) 53
BarI GAAGNNNNNNTAC 2 cut(s) 201, 233
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 4 cut(s) 681, 684, 884, 1083
BccI CCATC 3 cut(s) 176, 191, 1068
BceAI ACGGC 6 cut(s) 254, 287, 422, 523, 610, 851
BcgI CGANNNNNNTGC 2 cut(s) 710, 744
BciT130I CCWGG 1 cut(s) 685
BcoDI GTCTC 2 cut(s) 199, 735
BfaI CTAG 2 cut(s) 128, 1093
BfmI CTRYAG 1 cut(s) 574
BfoI RGCGCY 1 cut(s) 339
BfrI CTTAAG 1 cut(s) 785
BglI GCCNNNNNGGC 1 cut(s) 594
BlpI GCTNAGC 1 cut(s) 47
Bme1390I CCNGG 1 cut(s) 685
Bme18I GGWCC 2 cut(s) 559, 747
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 4 cut(s) 165, 559, 570, 747
BmiI GGNNCC 5 cut(s) 166, 318, 561, 679, 748
BmrFI CCNGG 1 cut(s) 685
BmsI GCATC 2 cut(s) 559, 1044
BpmI CTGGAG 1 cut(s) 1063
Bpu1102I GCTNAGC 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 932
BsaAI YACGTR 1 cut(s) 892
BsaHI GRCGYC 3 cut(s) 201, 408, 432
BsaJI CCNNGG 6 cut(s) 259, 312, 438, 684, 858, 867
BsaXI ACNNNNNCTCC 6 cut(s) 125, 155, 179, 209, 798, 828
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse118I RCCGGY 2 cut(s) 203, 644
Bse1I ACTGG 2 cut(s) 572, 630
BseBI CCWGG 1 cut(s) 685
BseDI CCNNGG 6 cut(s) 259, 312, 438, 684, 858, 867
BseGI GGATG 2 cut(s) 168, 550
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 2 cut(s) 572, 630
BseRI GAGGAG 2 cut(s) 10, 284
BseXI GCAGC 4 cut(s) 681, 684, 884, 1083
BseYI CCCAGC 2 cut(s) 147, 801
Bsh1236I CGCG 4 cut(s) 314, 364, 512, 668
BshFI GGCC 9 cut(s) 126, 167, 264, 515, 571, 588, 597, 719, 866
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 3 cut(s) 204, 645, 701
BslFI GGGAC 2 cut(s) 517, 760
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 2 cut(s) 199, 735
BsmBI CGTCTC 2 cut(s) 199, 735
BsmFI GGGAC 2 cut(s) 517, 760
BsnI GGCC 9 cut(s) 126, 167, 264, 515, 571, 588, 597, 719, 866
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 3 cut(s) 60, 101, 420
Bsp1720I GCTNAGC 1 cut(s) 47
BspANI GGCC 9 cut(s) 126, 167, 264, 515, 571, 588, 597, 719, 866
BspCNI CTCAG 1 cut(s) 39
BspFNI CGCG 4 cut(s) 314, 364, 512, 668
BspLI GGNNCC 5 cut(s) 166, 318, 561, 679, 748
BspPI GGATC 2 cut(s) 55, 96
BspTI CTTAAG 1 cut(s) 785
BsrBI CCGCTC 1 cut(s) 322
BsrFI RCCGGY 2 cut(s) 203, 644
BsrI ACTGG 2 cut(s) 572, 630
BssAI RCCGGY 2 cut(s) 203, 644
BssECI CCNNGG 6 cut(s) 259, 312, 438, 684, 858, 867
BssMI GATC 3 cut(s) 60, 101, 420
BssNI GRCGYC 3 cut(s) 201, 408, 432
Bst2UI CCWGG 1 cut(s) 685
Bst4CI ACNGT 2 cut(s) 280, 751
Bst6I CTCTTC 2 cut(s) 380, 981
BstACI GRCGYC 3 cut(s) 201, 408, 432
BstAFI CTTAAG 1 cut(s) 785
BstBAI YACGTR 1 cut(s) 892
BstC8I GCNNGC 6 cut(s) 76, 80, 205, 362, 650, 906
BstDEI CTNAG 1 cut(s) 47
BstDSI CCRYGG 3 cut(s) 312, 438, 867
BstF5I GGATG 2 cut(s) 168, 550
BstFNI CGCG 4 cut(s) 314, 364, 512, 668
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 6 cut(s) 338, 366, 512, 550, 910, 1059
BstKTI GATC 3 cut(s) 63, 104, 423
BstMAI GTCTC 2 cut(s) 199, 735
BstMBI GATC 3 cut(s) 60, 101, 420
BstMWI GCNNNNNNNGC 7 cut(s) 308, 344, 366, 585, 594, 798, 872
BstNI CCWGG 1 cut(s) 685
BstNSI RCATGY 1 cut(s) 908
BstSCI CCNGG 1 cut(s) 683
BstSFI CTRYAG 1 cut(s) 574
BstUI CGCG 4 cut(s) 314, 364, 512, 668
BstV1I GCAGC 4 cut(s) 681, 684, 884, 1083
BstXI CCANNNNNNTGG 1 cut(s) 1067
BsuRI GGCC 9 cut(s) 126, 167, 264, 515, 571, 588, 597, 719, 866
BtgI CCRYGG 3 cut(s) 312, 438, 867
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 2 cut(s) 168, 550
Cac8I GCNNGC 6 cut(s) 76, 80, 205, 362, 650, 906
CfoI GCGC 6 cut(s) 338, 366, 512, 550, 910, 1059
Cfr10I RCCGGY 2 cut(s) 203, 644
Cfr13I GGNCC 4 cut(s) 165, 559, 570, 747
Cfr42I CCGCGG 1 cut(s) 315
CseI GACGC 3 cut(s) 209, 416, 674
Csp6I GTAC 1 cut(s) 889
CviAII CATG 4 cut(s) 766, 905, 1036, 1075
CviQI GTAC 1 cut(s) 889
DdeI CTNAG 1 cut(s) 47
DpnI GATC 3 cut(s) 62, 103, 422
DpnII GATC 3 cut(s) 60, 101, 420
EaeI YGGCCR 2 cut(s) 717, 864
Eam1104I CTCTTC 2 cut(s) 380, 981
EarI CTCTTC 2 cut(s) 380, 981
EciI GGCGGA 3 cut(s) 133, 161, 385
Ecl136II GAGCTC 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 53
Eco47I GGWCC 2 cut(s) 559, 747
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 316
EcoICRI GAGCTC 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 928
EcoRII CCWGG 1 cut(s) 683
EcoT38I GRGCYC 1 cut(s) 53
Esp3I CGTCTC 2 cut(s) 199, 735
FaeI CATG 4 cut(s) 769, 908, 1039, 1078
FaiI YATR 8 cut(s) 576, 767, 822, 898, 906, 953, 1037, 1076
FaqI GGGAC 2 cut(s) 517, 760
FatI CATG 4 cut(s) 765, 904, 1035, 1074
FauI CCCGC 2 cut(s) 422, 543
FblI GTMKAC 1 cut(s) 273
FokI GGATG 2 cut(s) 155, 537
FriOI GRGCYC 1 cut(s) 53
FspBI CTAG 2 cut(s) 128, 1093
GlaI GCGC 6 cut(s) 337, 365, 511, 549, 909, 1058
GsaI CCCAGC 2 cut(s) 151, 805
GsuI CTGGAG 1 cut(s) 1063
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 9 cut(s) 126, 167, 264, 515, 571, 588, 597, 719, 866
HapII CCGG 3 cut(s) 204, 645, 701
HgaI GACGC 3 cut(s) 209, 416, 674
HhaI GCGC 6 cut(s) 338, 366, 512, 550, 910, 1059
Hin1I GRCGYC 3 cut(s) 201, 408, 432
Hin1II CATG 4 cut(s) 769, 908, 1039, 1078
Hin6I GCGC 6 cut(s) 336, 364, 510, 548, 908, 1057
HinP1I GCGC 6 cut(s) 336, 364, 510, 548, 908, 1057
HincII GTYRAC 3 cut(s) 274, 283, 499
HindII GTYRAC 3 cut(s) 274, 283, 499
HpaII CCGG 3 cut(s) 204, 645, 701
HphI GGTGA 2 cut(s) 244, 457
Hpy166II GTNNAC 6 cut(s) 274, 283, 499, 559, 664, 889
Hpy188I TCNGA 9 cut(s) 101, 199, 229, 505, 737, 855, 934, 970, 1053
Hpy188III TCNNGA 3 cut(s) 58, 490, 1042
Hpy8I GTNNAC 6 cut(s) 274, 283, 499, 559, 664, 889
Hpy99I CGWCG 9 cut(s) 164, 203, 275, 410, 434, 437, 509, 725, 741
HpyAV CCTTC 5 cut(s) 116, 227, 265, 395, 967
HpyCH4III ACNGT 2 cut(s) 280, 751
HpyCH4IV ACGT 6 cut(s) 87, 249, 432, 462, 606, 891
HpyCH4V TGCA 1 cut(s) 634
HpyF10VI GCNNNNNNNGC 7 cut(s) 308, 344, 366, 585, 594, 798, 872
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 6 cut(s) 87, 249, 432, 462, 606, 891
Hsp92I GRCGYC 3 cut(s) 201, 408, 432
Hsp92II CATG 4 cut(s) 769, 908, 1039, 1078
HspAI GCGC 6 cut(s) 336, 364, 510, 548, 908, 1057
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
KspI CCGCGG 1 cut(s) 315
Kzo9I GATC 3 cut(s) 60, 101, 420
LmnI GCTCC 4 cut(s) 316, 327, 677, 832
Lsp1109I GCAGC 4 cut(s) 681, 684, 884, 1083
LweI GCATC 2 cut(s) 559, 1044
MaeI CTAG 2 cut(s) 128, 1093
MaeII ACGT 6 cut(s) 87, 249, 432, 462, 606, 891
MaeIII GTNAC 3 cut(s) 208, 250, 463
MalI GATC 3 cut(s) 62, 103, 422
MbiI CCGCTC 1 cut(s) 322
MboI GATC 3 cut(s) 60, 101, 420
MboII GAAGA 2 cut(s) 367, 998
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 2 cut(s) 94, 928
MmeI TCCRAC 7 cut(s) 222, 252, 528, 663, 760, 878, 948
MreI CGCCGGCG 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 203
MseI TTAA 1 cut(s) 786
MspA1I CMGCKG 3 cut(s) 314, 801, 875
MspCI CTTAAG 1 cut(s) 785
MspI CCGG 3 cut(s) 204, 645, 701
MspR9I CCNGG 1 cut(s) 685
MvaI CCWGG 1 cut(s) 685
MvnI CGCG 4 cut(s) 314, 364, 512, 668
MwoI GCNNNNNNNGC 7 cut(s) 308, 344, 366, 585, 594, 798, 872
NaeI GCCGGC 1 cut(s) 205
NdeII GATC 3 cut(s) 60, 101, 420
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 4 cut(s) 769, 908, 1039, 1078
NlaIV GGNNCC 5 cut(s) 166, 318, 561, 679, 748
NmeAIII GCCGAG 3 cut(s) 240, 452, 524
NmuCI GTSAC 2 cut(s) 250, 463
NspI RCATGY 1 cut(s) 908
PaeI GCATGC 1 cut(s) 908
PceI AGGCCT 1 cut(s) 126
PcsI WCGNNNNNNNCGW 5 cut(s) 21, 267, 348, 429, 435
PdiI GCCGGC 1 cut(s) 205
PflFI GACNNNGTC 1 cut(s) 278
Ppu21I YACGTR 1 cut(s) 892
Psp124BI GAGCTC 1 cut(s) 53
Psp6I CCWGG 1 cut(s) 683
PspFI CCCAGC 2 cut(s) 147, 801
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 5 cut(s) 166, 318, 561, 679, 748
PspPI GGNCC 4 cut(s) 165, 559, 570, 747
PsyI GACNNNGTC 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 801
RsaI GTAC 1 cut(s) 890
RsaNI GTAC 1 cut(s) 889
SacI GAGCTC 1 cut(s) 53
SacII CCGCGG 1 cut(s) 315
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 1 cut(s) 786
Sau3AI GATC 3 cut(s) 60, 101, 420
Sau96I GGNCC 4 cut(s) 165, 559, 570, 747
ScrFI CCNGG 1 cut(s) 685
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 2 cut(s) 559, 1044
SfcI CTRYAG 1 cut(s) 574
SfiI GGCCNNNNNGGCC 1 cut(s) 594
Sfr303I CCGCGG 1 cut(s) 315
SgrAI CRCCGGYG 1 cut(s) 203
SgrBI CCGCGG 1 cut(s) 315
SinI GGWCC 2 cut(s) 559, 747
SmlI CTYRAG 2 cut(s) 785, 911
SmoI CTYRAG 2 cut(s) 785, 911
SphI GCATGC 1 cut(s) 908
Sse9I AATT 2 cut(s) 94, 928
SseBI AGGCCT 1 cut(s) 126
SspMI CTAG 2 cut(s) 128, 1093
SstI GAGCTC 1 cut(s) 53
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 683
TaaI ACNGT 2 cut(s) 280, 751
TaiI ACGT 6 cut(s) 90, 252, 435, 465, 609, 894
TaqI TCGA 1 cut(s) 273
TasI AATT 2 cut(s) 94, 928
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TseFI GTSAC 2 cut(s) 250, 463
TseI GCWGC 4 cut(s) 694, 697, 872, 1071
Tsp45I GTSAC 2 cut(s) 250, 463
TspDTI ATGAA 6 cut(s) 28, 145, 475, 782, 1024, 1091
TspGWI ACGGA 5 cut(s) 13, 181, 508, 690, 845
Tth111I GACNNNGTC 1 cut(s) 278
Vha464I CTTAAG 1 cut(s) 785
VpaK11BI GGWCC 2 cut(s) 559, 747
XapI RAATTY 1 cut(s) 928
XceI RCATGY 1 cut(s) 908
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 2 cut(s) 128, 1093
ZraI GACGTC 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.