Rw4G033720
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009489
Physical Location & Seq
Reverse (-)
60858844 .. 60861594
2751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033720.1

Sequence Viewer

Length: 1215 bp
ATGGCATACCCAGAAGAAGAACATCCTCGGCTTCTTCTCCACAACTTCCTTTCTGTCCAGGAATGCAAGGAACTGGAATATATACACAAAAGCAATTGCACTGTGGGTTACAGACCCAATGTCTTCTCAACTACTCTTTCACATCTCATAGCCACCAACTGTGCCCATCTCATCATGCCCTTCGTCCCCATCAGAGAGAGGTTGAAGGAGAAGGTAGAGGAATTCTTTGGCTGCGAGTATGAACTCTTTGTTGAATTCACCGGTTTGATCAGACTACACTGTGATGATACTTTTCAATTTTCGTCGTATATCAGCTGGAGTAGAGGATCAACCATTGGGTGGCATAGTGATGACAACAGGCCCTATCTCAAACAGCGTGACTTTGCGGCGGTTTGTTATTTGAACAATTACGGGGATGATTTCAAAGGTGGACTTTTTCACTTCCAGGATGGGGATCTAACAACTATTGTTCCATCAAGTGGGGATGTTGTGATATACACAGCTGACAGCCGCAACACTCATTCTGTTGATGAGATTACTGATGGGGAAAGGGTCACAATGGCATTATGGTTCAGTCGTGATGCAGCCCATGATGAAGATGCTAAACTTATTTCGCTTCTCTCACAGACTCCATTACGAGATAATGGTCCTGAATTATGGCTACCTTTGCCAGGATCGAGTAATATGTACTGGTTTTCACCAGAACAAGCTTCGTCCCATGATCGGTTGGGATTTGATATATGCTGTGCAAGACTGCATGTTCTTGGATATGATGTAGTATACTCTGAAGACAAGATCTCCTGTTCGAATATTTCTGAGTTACAGATGGAGAAGTTGCGATTAGCAAGGGGAGATGAGTTGTTTGACTATGAGTTTGTCAACATCTTGCACGCACTTCAGGTAGTGCAATTCTACTGTTGGAAAGCTTCTGATCTGAAATCTACTAAAGTAGAAGATAGCAATAAGGTGGTACTCTTATTGCAATCACAGCGGGAGAAATCTCTCTGCCTCAAGTCTTTATTTCTGAAGGATCTTCATCTAGCAGAGTCTGTATTTTCTGCTGAGAGTGCAAAACGTCCCAATTGGGCCCATCTTACAGTTGCAGTTGCTATGTGGGAACATTATGCTCACAGGTTACATAAAGAACTGGTAATGTGCTTACCTCACTGGAAAACACATCAATCTATATTTCATGTATCATTTGATGACAATTGA

Protein Analysis

404

Amino Acids

46.66

Weight (kDa)

5.51

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 99 - 191 3.6e-12 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 339, 479
AccI GTMKAC 1 cut(s) 780
AciI CCGC 4 cut(s) 386, 389, 511, 991
AclWI GGATC 4 cut(s) 334, 462, 682, 1038
AcsI RAATTY 2 cut(s) 221, 254
AcuI CTGAAG 3 cut(s) 807, 881, 1046
AfaI GTAC 2 cut(s) 689, 972
AfiI CCNNNNNNNGG 5 cut(s) 339, 451, 479, 671, 723
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 5 cut(s) 205, 254, 296, 403, 424
AhdI GACNNNNNGTC 1 cut(s) 119
AjnI CCWGG 3 cut(s) 57, 444, 670
AluBI AGCT 4 cut(s) 315, 503, 710, 926
AluI AGCT 4 cut(s) 315, 503, 710, 926
AlwI GGATC 4 cut(s) 334, 462, 682, 1038
AlwNI CAGNNNCTG 1 cut(s) 1049
AoxI GGCC 2 cut(s) 359, 1086
ApaI GGGCCC 1 cut(s) 1090
ApeKI GCWGC 2 cut(s) 231, 584
ApoI RAATTY 2 cut(s) 221, 254
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
AsiGI ACCGGT 1 cut(s) 260
AspS9I GGNCC 4 cut(s) 360, 647, 1086, 1087
AsuHPI GGTGA 2 cut(s) 250, 690
AsuII TTCGAA 1 cut(s) 806
AvaII GGWCC 1 cut(s) 647
BaeGI GKGCMC 2 cut(s) 166, 1090
BanII GRGCYC 1 cut(s) 1090
BbsI GAAGAC 2 cut(s) 115, 795
BbvI GCAGC 2 cut(s) 218, 596
BccI CCATC 7 cut(s) 174, 197, 443, 481, 536, 820, 1098
BciT130I CCWGG 3 cut(s) 59, 446, 672
BclI TGATCA 1 cut(s) 267
BfaI CTAG 1 cut(s) 1040
BglII AGATCT 1 cut(s) 795
BisI GCNGC 4 cut(s) 232, 387, 511, 585
BlsI GCNGC 4 cut(s) 233, 388, 512, 586
Bme1390I CCNGG 3 cut(s) 59, 446, 672
Bme18I GGWCC 1 cut(s) 647
BmeRI GACNNNNNGTC 1 cut(s) 119
BmgT120I GGNCC 4 cut(s) 360, 647, 1086, 1087
BmiI GGNNCC 1 cut(s) 1088
BmrFI CCNGG 3 cut(s) 59, 446, 672
BmsI GCATC 2 cut(s) 571, 589
BpiI GAAGAC 2 cut(s) 115, 795
BpmI CTGGAG 1 cut(s) 337
Bpu14I TTCGAA 1 cut(s) 806
BpuEI CTTGAG 1 cut(s) 995
BsaBI GATNNNNATC 2 cut(s) 453, 1035
BsaJI CCNNGG 1 cut(s) 26
BsaWI WCCGGW 1 cut(s) 260
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 5 cut(s) 339, 451, 479, 671, 723
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 4 cut(s) 78, 695, 1152, 1172
Bse8I GATNNNNATC 2 cut(s) 453, 1035
BseBI CCWGG 3 cut(s) 59, 446, 672
BseDI CCNNGG 1 cut(s) 26
BseGI GGATG 4 cut(s) 22, 421, 454, 490
BseJI GATNNNNATC 2 cut(s) 453, 1035
BseLI CCNNNNNNNGG 5 cut(s) 339, 451, 479, 671, 723
BseMII CTCAG 2 cut(s) 807, 1053
BseNI ACTGG 4 cut(s) 78, 695, 1152, 1172
BseSI GKGCMC 2 cut(s) 166, 1090
BseXI GCAGC 2 cut(s) 218, 596
BshFI GGCC 2 cut(s) 361, 1088
BshTI ACCGGT 1 cut(s) 260
BsiSI CCGG 1 cut(s) 261
BslFI GGGAC 3 cut(s) 170, 700, 1062
BslI CCNNNNNNNGG 5 cut(s) 339, 451, 479, 671, 723
BsmFI GGGAC 3 cut(s) 170, 700, 1062
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 2 cut(s) 361, 1088
Bsp119I TTCGAA 1 cut(s) 806
Bsp120I GGGCCC 1 cut(s) 1086
Bsp1286I GDGCHC 2 cut(s) 166, 1090
Bsp143I GATC 8 cut(s) 267, 326, 454, 674, 721, 795, 931, 1030
BspACI CCGC 4 cut(s) 386, 389, 511, 991
BspANI GGCC 2 cut(s) 361, 1088
BspCNI CTCAG 2 cut(s) 808, 1054
BspLI GGNNCC 1 cut(s) 1088
BspPI GGATC 4 cut(s) 334, 462, 682, 1038
BspT104I TTCGAA 1 cut(s) 806
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 4 cut(s) 78, 695, 1152, 1172
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 26
BssMI GATC 8 cut(s) 267, 326, 454, 674, 721, 795, 931, 1030
BssNAI GTATAC 1 cut(s) 781
Bst1107I GTATAC 1 cut(s) 781
Bst2UI CCWGG 3 cut(s) 59, 446, 672
Bst4CI ACNGT 5 cut(s) 103, 161, 281, 917, 1099
BstBI TTCGAA 1 cut(s) 806
BstC8I GCNNGC 1 cut(s) 891
BstDEI CTNAG 2 cut(s) 816, 1062
BstENI CCTNNNNNAGG 1 cut(s) 669
BstF5I GGATG 4 cut(s) 22, 421, 454, 490
BstKTI GATC 8 cut(s) 270, 329, 457, 677, 724, 798, 934, 1033
BstMBI GATC 8 cut(s) 267, 326, 454, 674, 721, 795, 931, 1030
BstMWI GCNNNNNNNGC 3 cut(s) 667, 988, 1067
BstNI CCWGG 3 cut(s) 59, 446, 672
BstNSI RCATGY 1 cut(s) 761
BstSCI CCNGG 3 cut(s) 57, 444, 670
BstSLI GKGCMC 2 cut(s) 166, 1090
BstV1I GCAGC 2 cut(s) 218, 596
BstV2I GAAGAC 2 cut(s) 115, 795
BstX2I RGATCY 3 cut(s) 454, 795, 1030
BstYI RGATCY 3 cut(s) 454, 795, 1030
BstZ17I GTATAC 1 cut(s) 781
BsuRI GGCC 2 cut(s) 361, 1088
BtsCI GGATG 4 cut(s) 22, 421, 454, 490
BtsIMutI CAGTG 3 cut(s) 99, 277, 1165
Cac8I GCNNGC 1 cut(s) 891
CaiI CAGNNNCTG 1 cut(s) 1049
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 4 cut(s) 360, 647, 1086, 1087
Csp6I GTAC 2 cut(s) 688, 971
CspAI ACCGGT 1 cut(s) 260
CviAII CATG 5 cut(s) 175, 590, 719, 758, 1193
CviQI GTAC 2 cut(s) 688, 971
DdeI CTNAG 2 cut(s) 816, 1062
DpnI GATC 8 cut(s) 269, 328, 456, 676, 723, 797, 933, 1032
DpnII GATC 8 cut(s) 267, 326, 454, 674, 721, 795, 931, 1030
DriI GACNNNNNGTC 1 cut(s) 119
Eam1105I GACNNNNNGTC 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 1090
Eco47I GGWCC 1 cut(s) 647
Eco57I CTGAAG 3 cut(s) 807, 881, 1046
EcoNI CCTNNNNNAGG 1 cut(s) 669
EcoO109I RGGNCCY 1 cut(s) 360
EcoRI GAATTC 2 cut(s) 221, 254
EcoRII CCWGG 3 cut(s) 57, 444, 670
EcoT38I GRGCYC 1 cut(s) 1090
FaeI CATG 5 cut(s) 178, 593, 722, 761, 1196
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 3 cut(s) 170, 700, 1062
FatI CATG 5 cut(s) 174, 589, 718, 757, 1192
FauI CCCGC 1 cut(s) 984
FbaI TGATCA 1 cut(s) 267
FblI GTMKAC 1 cut(s) 780
Fnu4HI GCNGC 4 cut(s) 232, 387, 511, 585
FokI GGATG 4 cut(s) 9, 428, 461, 497
FriOI GRGCYC 1 cut(s) 1090
Fsp4HI GCNGC 4 cut(s) 232, 387, 511, 585
FspBI CTAG 1 cut(s) 1040
GluI GCNGC 4 cut(s) 232, 387, 511, 585
GsuI CTGGAG 1 cut(s) 337
HaeIII GGCC 2 cut(s) 361, 1088
HapII CCGG 1 cut(s) 261
Hin1II CATG 5 cut(s) 178, 593, 722, 761, 1196
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 2 cut(s) 708, 924
HinfI GANTC 2 cut(s) 628, 1046
HpaII CCGG 1 cut(s) 261
HphI GGTGA 2 cut(s) 250, 690
Hpy166II GTNNAC 3 cut(s) 431, 781, 880
Hpy188I TCNGA 7 cut(s) 194, 272, 787, 817, 931, 936, 1026
Hpy188III TCNNGA 2 cut(s) 578, 650
Hpy8I GTNNAC 3 cut(s) 431, 781, 880
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 4 cut(s) 190, 199, 205, 1021
HpyCH4III ACNGT 5 cut(s) 103, 161, 281, 917, 1099
HpyCH4IV ACGT 1 cut(s) 1075
HpyF10VI GCNNNNNNNGC 3 cut(s) 667, 988, 1067
HpyF3I CTNAG 2 cut(s) 816, 1062
HpySE526I ACGT 1 cut(s) 1075
Hsp92II CATG 5 cut(s) 178, 593, 722, 761, 1196
Ksp22I TGATCA 1 cut(s) 267
Kzo9I GATC 8 cut(s) 267, 326, 454, 674, 721, 795, 931, 1030
Lsp1109I GCAGC 2 cut(s) 218, 596
LweI GCATC 2 cut(s) 571, 589
MaeI CTAG 1 cut(s) 1040
MaeII ACGT 1 cut(s) 1075
MaeIII GTNAC 5 cut(s) 107, 377, 553, 819, 1134
MalI GATC 8 cut(s) 269, 328, 456, 676, 723, 797, 933, 1032
MboI GATC 8 cut(s) 267, 326, 454, 674, 721, 795, 931, 1030
MboII GAAGA 8 cut(s) 26, 26, 29, 115, 608, 800, 965, 1025
MfeI CAATTG 3 cut(s) 94, 1081, 1210
MflI RGATCY 3 cut(s) 454, 795, 1030
MhlI GDGCHC 2 cut(s) 166, 1090
MluCI AATT 9 cut(s) 94, 221, 254, 296, 406, 653, 908, 1081, 1210
MlyI GAGTC 2 cut(s) 622, 1055
MmeI TCCRAC 1 cut(s) 899
MnlI CCTC 6 cut(s) 36, 192, 211, 317, 1019, 1173
MslI CAYNNNNRTG 2 cut(s) 282, 348
MspA1I CMGCKG 3 cut(s) 315, 503, 991
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 3 cut(s) 59, 446, 672
MunI CAATTG 3 cut(s) 94, 1081, 1210
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 3 cut(s) 59, 446, 672
MwoI GCNNNNNNNGC 3 cut(s) 667, 988, 1067
NdeII GATC 8 cut(s) 267, 326, 454, 674, 721, 795, 931, 1030
NlaIII CATG 5 cut(s) 178, 593, 722, 761, 1196
NlaIV GGNNCC 1 cut(s) 1088
NmeAIII GCCGAG 1 cut(s) 7
NmuCI GTSAC 2 cut(s) 377, 553
NspI RCATGY 1 cut(s) 761
NspV TTCGAA 1 cut(s) 806
PctI GAATGC 1 cut(s) 68
PflMI CCANNNNNTGG 2 cut(s) 339, 479
PfoI TCCNGGA 2 cut(s) 57, 444
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 4 cut(s) 233, 388, 512, 586
PleI GAGTC 2 cut(s) 622, 1054
PpsI GAGTC 2 cut(s) 622, 1054
Psp6I CCWGG 3 cut(s) 57, 444, 670
PspGI CCWGG 3 cut(s) 57, 444, 670
PspN4I GGNNCC 1 cut(s) 1088
PspOMI GGGCCC 1 cut(s) 1086
PspPI GGNCC 4 cut(s) 360, 647, 1086, 1087
PstNI CAGNNNCTG 1 cut(s) 1049
PsuI RGATCY 3 cut(s) 454, 795, 1030
PvuII CAGCTG 2 cut(s) 315, 503
RsaI GTAC 2 cut(s) 689, 972
RsaNI GTAC 2 cut(s) 688, 971
RseI CAYNNNNRTG 2 cut(s) 282, 348
SatI GCNGC 4 cut(s) 232, 387, 511, 585
Sau3AI GATC 8 cut(s) 267, 326, 454, 674, 721, 795, 931, 1030
Sau96I GGNCC 4 cut(s) 360, 647, 1086, 1087
SchI GAGTC 2 cut(s) 622, 1055
ScrFI CCNGG 3 cut(s) 59, 446, 672
SduI GDGCHC 2 cut(s) 166, 1090
SfaNI GCATC 2 cut(s) 571, 589
SfuI TTCGAA 1 cut(s) 806
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 2 cut(s) 282, 348
SmlI CTYRAG 1 cut(s) 1010
SmoI CTYRAG 1 cut(s) 1010
Sse9I AATT 9 cut(s) 94, 221, 254, 296, 406, 653, 908, 1081, 1210
SsiI CCGC 4 cut(s) 386, 389, 511, 991
SspI AATATT 1 cut(s) 811
SspMI CTAG 1 cut(s) 1040
StyD4I CCNGG 3 cut(s) 57, 444, 670
TaaI ACNGT 5 cut(s) 103, 161, 281, 917, 1099
TaiI ACGT 1 cut(s) 1078
TaqI TCGA 2 cut(s) 677, 806
TasI AATT 9 cut(s) 94, 221, 254, 296, 406, 653, 908, 1081, 1210
TatI WGTACW 1 cut(s) 687
TauI GCSGC 2 cut(s) 389, 513
TscAI CASTG 3 cut(s) 106, 284, 1172
TseFI GTSAC 2 cut(s) 377, 553
TseI GCWGC 2 cut(s) 231, 584
Tsp45I GTSAC 2 cut(s) 377, 553
TspDTI ATGAA 4 cut(s) 255, 609, 1025, 1181
TspRI CASTG 3 cut(s) 106, 284, 1172
Van91I CCANNNNNTGG 2 cut(s) 339, 479
VpaK11BI GGWCC 1 cut(s) 647
XagI CCTNNNNNAGG 1 cut(s) 669
XapI RAATTY 2 cut(s) 221, 254
XceI RCATGY 1 cut(s) 761
XmiI GTMKAC 1 cut(s) 780
XspI CTAG 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.