Rw5G004920

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0019013
Physical Location & Seq
Forward (+)
5100618 .. 5104802
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G004920.1

Sequence Viewer

Length: 948 bp
ATGGAAACCGACGAAGAAGAACAACGCGTCACTCCATTCCAGCTCCAGTTCGAGAAGCCAGTCGCTTTGCAGGTTAAGATCGCAGGGTGGAATCCCGAGAAGGATTTACTCGCCATGAGGCTCTGGACAATCTCCCCAGGGAAGAGCATAACGTCTTTGTGTTGGAGACCTGATGGTAAAGCCGTCGCCGTCGGGCTCGAAGATGGAACAGTCTCATTGCATGATGTTGAATTTTCAAACTTCTTCAATTGGCTCTTGAAGTGTATAAAGTTACTCAATTCAGAACCCAGTGATCACCATCTTCTACTGTACAATAGTGAACTTGTTATATTCTTGAAGTTTCTATATGGCCAAGATCCTGTTAAGCAGTTGCTGGAGGTGTCGGAAGCTGATGAGAGTATCGAAGTTGGATTGGAAACAGTGCAGAGAGTTAGAGAATTAGTTCAGTTTGGGGGAGTTTCAGATTGTGAGCACCTGCAGAGGACGTTAGCCAAGGAATTTCAGCAGATGGAGTCTAGTTTTAAGGAGGCTTTTCAGATGCCCTTTACTACAATTTCAAGAAAGATAGCTGTGAAGATTCATTGCCACTTTGCCCACTTCCACCTTTGTCAGCATCTTTGTCTACCAGTATTCCTATGTCAGTATCATATTATGAGGATGCCTCCCATTCCGTCCCTTACTCGTCAACATATGTTTCTTGAAAGTCAGATTGTTTTATTATTAAATGAAACAACTACCACTTCTGAAAGCTCTGGTGATGCCTGTATGATGATTGTGCAAGCAAATGACCTTCCATATGTATCTATCACAAGATCCACTAGTCTAAACTATTGGAACTTGCATCAACTGAAGGACTCTGTTGTTCACTTGCAAATGAAGAATGAGAAGGTTCGCAGTATACCCCATTCTGTTATTGCTCCTCTCGCAGTTAGCTGTAAGTCGCTCTAA

Protein Analysis

315

Amino Acids

36.04

Weight (kDa)

5.95

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC4_WD40 PF12894 40 - 79 7.6e-08 Anaphase-promoting complex subunit 4 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019013)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0006431
rosa_laevigata RLG00000031360
rosa_multiflora Rmu_co8360215.1_g000001 Rmu_sc0008991.1_g000006 Rmu_sc0036442.1_g000001
rosa_roxburghii Rroxscaffold_1G00069550
rosa_rugosa Rorug05G0252100
rosa_samantha Rh5CG363500
rosa_wichuraiana Rw5G004920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 483
Acc36I ACCTGC 2 cut(s) 61, 483
AccI GTMKAC 2 cut(s) 622, 898
AccII CGCG 1 cut(s) 27
AclWI GGATC 2 cut(s) 350, 807
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 2 cut(s) 230, 497
AcuI CTGAAG 1 cut(s) 869
AfaI GTAC 1 cut(s) 311
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 7 cut(s) 230, 237, 247, 259, 337, 558, 701
AhlI ACTAGT 1 cut(s) 818
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 5 cut(s) 43, 389, 569, 750, 933
AluI AGCT 5 cut(s) 43, 389, 569, 750, 933
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 2 cut(s) 160, 217
AlwI GGATC 2 cut(s) 350, 807
AlwNI CAGNNNCTG 1 cut(s) 373
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 1 cut(s) 349
ApoI RAATTY 2 cut(s) 230, 497
Asp700I GAANNNNTTC 1 cut(s) 441
AsuHPI GGTGA 2 cut(s) 287, 767
AvaI CYCGRG 1 cut(s) 95
BalI TGGCCA 1 cut(s) 351
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 474
BccI CCATC 4 cut(s) 167, 197, 306, 502
BceAI ACGGC 2 cut(s) 167, 173
BciT130I CCWGG 1 cut(s) 138
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 160, 217
BcuI ACTAGT 1 cut(s) 818
BfaI CTAG 2 cut(s) 516, 819
BfmI CTRYAG 1 cut(s) 476
BfuAI ACCTGC 2 cut(s) 61, 483
Bme1390I CCNGG 1 cut(s) 138
BmeT110I CYCGRG 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 138
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 5 cut(s) 528, 622, 648, 748, 850
BmuI ACTGGG 1 cut(s) 282
BplI GAGNNNNNCTC 2 cut(s) 646, 678
BpmI CTGGAG 2 cut(s) 29, 395
BsaBI GATNNNNATC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 160
BsaJI CCNNGG 3 cut(s) 136, 137, 492
Bse1I ACTGG 4 cut(s) 46, 59, 288, 626
Bse3DI GCAATG 2 cut(s) 215, 580
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 136, 137, 492
BseGI GGATG 1 cut(s) 663
BseJI GATNNNNATC 1 cut(s) 297
BseMI GCAATG 2 cut(s) 215, 580
BseNI ACTGG 4 cut(s) 46, 59, 288, 626
BseRI GAGGAG 1 cut(s) 909
BsgI GTGCAG 1 cut(s) 443
Bsh1236I CGCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 95
BslFI GGGAC 1 cut(s) 658
BsmAI GTCTC 2 cut(s) 160, 217
BsmFI GGGAC 1 cut(s) 658
BsnI GGCC 1 cut(s) 351
Bso31I GGTCTC 1 cut(s) 160
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 198, 474
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 4 cut(s) 78, 292, 355, 812
BspANI GGCC 1 cut(s) 351
BspFNI CGCG 1 cut(s) 27
BspMAI CTGCAG 1 cut(s) 480
BspMI ACCTGC 2 cut(s) 61, 483
BspPI GGATC 2 cut(s) 350, 807
BspQI GCTCTTC 1 cut(s) 137
BspTNI GGTCTC 1 cut(s) 160
BsrDI GCAATG 2 cut(s) 215, 580
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 4 cut(s) 46, 59, 288, 626
BssECI CCNNGG 3 cut(s) 136, 137, 492
BssMI GATC 4 cut(s) 78, 292, 355, 812
BssNAI GTATAC 1 cut(s) 899
BssT1I CCWWGG 1 cut(s) 492
Bst1107I GTATAC 1 cut(s) 899
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 3 cut(s) 211, 309, 421
Bst6I CTCTTC 1 cut(s) 137
BstAUI TGTACA 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 780
BstF5I GGATG 1 cut(s) 663
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 4 cut(s) 81, 295, 358, 815
BstMAI GTCTC 2 cut(s) 160, 217
BstMBI GATC 4 cut(s) 78, 292, 355, 812
BstMWI GCNNNNNNNGC 1 cut(s) 923
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 1 cut(s) 476
BstUI CGCG 1 cut(s) 27
BstX2I RGATCY 2 cut(s) 355, 812
BstYI RGATCY 2 cut(s) 355, 812
BstZ17I GTATAC 1 cut(s) 899
BsuRI GGCC 1 cut(s) 351
BtsCI GGATG 1 cut(s) 663
BtsIMutI CAGTG 2 cut(s) 295, 426
BveI ACCTGC 2 cut(s) 61, 483
Cac8I GCNNGC 1 cut(s) 780
CaiI CAGNNNCTG 1 cut(s) 373
CseI GACGC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 310
CviAII CATG 2 cut(s) 115, 221
CviQI GTAC 1 cut(s) 310
DpnI GATC 4 cut(s) 80, 294, 357, 814
DpnII GATC 4 cut(s) 78, 292, 355, 812
EaeI YGGCCR 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 869
Eco88I CYCGRG 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 492
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 2 cut(s) 118, 224
FaqI GGGAC 1 cut(s) 658
FatI CATG 2 cut(s) 114, 220
FauNDI CATATG 2 cut(s) 690, 796
FbaI TGATCA 1 cut(s) 292
FblI GTMKAC 2 cut(s) 622, 898
FokI GGATG 1 cut(s) 670
FriOI GRGCYC 1 cut(s) 198
FspBI CTAG 2 cut(s) 516, 819
GsuI CTGGAG 2 cut(s) 29, 395
HaeIII GGCC 1 cut(s) 351
HgaI GACGC 1 cut(s) 16
Hin1II CATG 2 cut(s) 118, 224
HincII GTYRAC 1 cut(s) 686
HindII GTYRAC 1 cut(s) 686
HinfI GANTC 4 cut(s) 91, 512, 577, 854
HphI GGTGA 2 cut(s) 287, 767
Hpy166II GTNNAC 5 cut(s) 320, 623, 686, 865, 899
Hpy188I TCNGA 6 cut(s) 283, 385, 463, 537, 708, 745
Hpy188III TCNNGA 7 cut(s) 52, 95, 124, 256, 334, 558, 698
Hpy8I GTNNAC 5 cut(s) 320, 623, 686, 865, 899
Hpy99I CGWCG 3 cut(s) 14, 188, 194
HpyAV CCTTC 4 cut(s) 94, 800, 844, 880
HpyCH4III ACNGT 3 cut(s) 211, 309, 421
HpyCH4IV ACGT 2 cut(s) 152, 485
HpyCH4V TGCA 7 cut(s) 70, 220, 424, 478, 778, 841, 871
HpyF10VI GCNNNNNNNGC 1 cut(s) 923
HpySE526I ACGT 2 cut(s) 152, 485
Hsp92II CATG 2 cut(s) 118, 224
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 4 cut(s) 78, 292, 355, 812
LguI GCTCTTC 1 cut(s) 137
LmnI GCTCC 2 cut(s) 48, 922
LweI GCATC 5 cut(s) 528, 622, 648, 748, 850
MaeI CTAG 2 cut(s) 516, 819
MaeII ACGT 2 cut(s) 152, 485
MaeIII GTNAC 2 cut(s) 28, 270
MalI GATC 4 cut(s) 80, 294, 357, 814
MboI GATC 4 cut(s) 78, 292, 355, 812
MboII GAAGA 8 cut(s) 26, 29, 154, 212, 235, 293, 586, 889
MfeI CAATTG 1 cut(s) 247
MflI RGATCY 2 cut(s) 355, 812
MhlI GDGCHC 2 cut(s) 198, 474
MlsI TGGCCA 1 cut(s) 351
MluCI AATT 6 cut(s) 230, 247, 277, 437, 497, 552
MluI ACGCGT 1 cut(s) 25
MluNI TGGCCA 1 cut(s) 351
MlyI GAGTC 2 cut(s) 521, 848
MmeI TCCRAC 3 cut(s) 143, 363, 388
MnlI CCTC 7 cut(s) 111, 370, 474, 520, 648, 672, 930
Mox20I TGGCCA 1 cut(s) 351
MroXI GAANNNNTTC 1 cut(s) 441
MscI TGGCCA 1 cut(s) 351
MseI TTAA 4 cut(s) 75, 363, 522, 722
Msp20I TGGCCA 1 cut(s) 351
MspR9I CCNGG 1 cut(s) 138
MunI CAATTG 1 cut(s) 247
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 923
NdeI CATATG 2 cut(s) 690, 796
NdeII GATC 4 cut(s) 78, 292, 355, 812
NlaIII CATG 2 cut(s) 118, 224
NmuCI GTSAC 1 cut(s) 28
PaqCI CACCTGC 1 cut(s) 483
PasI CCCWGGG 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 137
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 2 cut(s) 91, 577
PleI GAGTC 2 cut(s) 520, 848
PpsI GAGTC 2 cut(s) 520, 848
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PstI CTGCAG 1 cut(s) 480
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 2 cut(s) 355, 812
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 137
SaqAI TTAA 4 cut(s) 75, 363, 522, 722
Sau3AI GATC 4 cut(s) 78, 292, 355, 812
SchI GAGTC 2 cut(s) 521, 848
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 2 cut(s) 198, 474
SfaNI GCATC 5 cut(s) 528, 622, 648, 748, 850
SfcI CTRYAG 1 cut(s) 476
SpeI ACTAGT 1 cut(s) 818
Sse9I AATT 6 cut(s) 230, 247, 277, 437, 497, 552
SspMI CTAG 2 cut(s) 516, 819
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 3 cut(s) 211, 309, 421
TaiI ACGT 2 cut(s) 155, 488
TaqI TCGA 3 cut(s) 51, 198, 402
TasI AATT 6 cut(s) 230, 247, 277, 437, 497, 552
TatI WGTACW 1 cut(s) 309
TfiI GAWTC 2 cut(s) 91, 577
Tru1I TTAA 4 cut(s) 75, 363, 522, 722
Tru9I TTAA 4 cut(s) 75, 363, 522, 722
TscAI CASTG 2 cut(s) 295, 426
TseFI GTSAC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 3 cut(s) 569, 741, 890
TspGWI ACGGA 1 cut(s) 660
TspRI CASTG 2 cut(s) 295, 426
XapI RAATTY 2 cut(s) 230, 497
XmiI GTMKAC 2 cut(s) 622, 898
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 2 cut(s) 516, 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.