Rw5G033230

NAD-dependent protein deacetylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013314
Physical Location & Seq
Forward (+)
56401752 .. 56409951
8200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033230.1

Sequence Viewer

Length: 1461 bp
ATGACGCGCTGCCTCAGCCATCAAGCTAGCCCTTACAAATCTGACCAGATCTCCATGCCCGGCCCACTGAGATTTCAAGTTTCCAGTGTGATCGACCGGAGATTTGAGTCGCCCTCTCTTCGGTCTGATTCGCACACCCGGATCCCAAGTATTGAACAGCTTGCCCTGCTGGTACAGAAGAGTAAGCATCTAGTGGTGTTTACAGGTGCAGGAATATCAACTTCTTGTGGTATACCTGATTTTCGAGGTCCAAAGGGAATTTGGACTCTTCAGCGTGAAGGCAAAGCCTTGCCAGAAGCATCATTGCCATTTCATCGTGCAATGCCGAGCTTGACTCATATGGCTTTGGTTGTGCTGGAGAGGGCTGGCATACTGAAGTTTGTTATTAGCCAGAATGTTGACGGCCTCCATCTTCGATCTGGAATACCAAGGAAGAAACTTGCGGAGTTGCATGGTAACTCCTTTATGGAAGTCTGCCCTTCTTGTGGCATCGAGTATATGCGGGATTTTGAGGTAGAAACTATTGGCCTAAAGGAGACTTCCAGAAGGTGCTCAGATAAAAAGTGTGGAGCTAGGCTTAAGGATACAGTTCTTGACTGGGAGGATGCATTGCCCTCAAAGGAGATGAATCAGGCTGAAAAGCACTGCAGAATGGCTGATGTTGTTTTATGTTTGGGAACAAGTTTGCAGATAACACCAGCATGCAACCTACCTCTGAGATCACTCCGTGGTGGGGGAAAGATTGTAATTGTTAATCTTCAGAAAACTCCAAAGGACAAGAAGGCAACTTTACTGCTCCATGGGCTTGTAGATAAGGTTATTTCAGGTGTCTTGGACAGTCTAAATCTTCAGATTCCTCCATTTGTTCGGATAGATCTTTTTCAGATCATTCTAACTCAAGCATTGAGTATAGATGAAAAGTATGTGAACTGGAACCTCCGGGTAGCAAGTGTACATGGACTTAAAGCTCCATTGCCATTCATCAAATCTATTGAGATATCCTTCGTAGACAATCAAGATTATAAAGCAGCTATTCTACAGAACGAGCCATTTCAATTGAAAAGGAGAACCTCACAGTCAAAATCAATTGAAATGGTTTTGAAATTCAACTTCATTGATGGTTGTGGCTGTCCATTCACAGAGATTAACGTGTCTCTCAATTGGGAGGTTTCAACAGACCATTCTAAGCTTGATAAAGATGCCATATTGCAAAAGCTCAGAGACACAGCTACTGATGAATCATGCTGCGGCAAGAATGCTGTTGTTGAGAGAAATTTCACCCCTAGCCCAAAAACTGAGGTTCTTACGTATGCCATTGTAACCAACGTTGTCACATATAAGAAGACCACTGAAGCTGTGCAAGCTGACACTCTAAGTAATGGGGTTAAAAGGCGGAAGAACGATGGTCCTGCAACATCTAAGAAACGATCTAAAGTGCAAAGGCGCAAATCTAGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 ko05230 map04714 map05230
Pfam Domains
Protein Families
SIR2

Protein Analysis

486

Amino Acids

54.2

Weight (kDa)

9.46

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SIR2 PF02146 104 - 233 7.9e-21 Sir2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013314)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55760
fragaria_vesca FvH4_3g29170 FvH4_3g29170
malus_domestica MD03G1179400.v1.1 MD11G1199100.v1.1
prunus_persica Prupe.4G243700_v2.0.a1 Prupe.4G243700_v2.0.a1
pyrus_communis pycom03g13540 pycom11g17170
rosa_chinensis RchiOBHm_Chr5g0053471
rosa_laevigata RLG00000034885
rosa_roxburghii Rroxscaffold_1G00026940
rosa_rugosa Rorug05G0280200
rosa_samantha Rh5AG351400 Rh5BG363600 Rh5DG378300 Rh7BG051900
rosa_wichuraiana Rw5G033230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
AccI GTMKAC 2 cut(s) 232, 1008
AccII CGCG 1 cut(s) 7
AciI CCGC 4 cut(s) 443, 502, 1248, 1393
AclI AACGTT 1 cut(s) 1326
AclWI GGATC 2 cut(s) 136, 149
AcsI RAATTY 3 cut(s) 258, 1103, 1273
AcuI CTGAAG 5 cut(s) 254, 395, 743, 833, 1371
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 2 cut(s) 174, 954
AfiI CCNNNNNNNGG 3 cut(s) 120, 485, 733
AflII CTTAAG 1 cut(s) 578
AflIII ACRYGT 1 cut(s) 1149
AgsI TTSAA 8 cut(s) 77, 155, 1055, 1060, 1091, 1102, 1108, 1173
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 3 cut(s) 530, 1158, 1215
AlwI GGATC 2 cut(s) 136, 149
AlwNI CAGNNNCTG 1 cut(s) 1232
AoxI GGCC 3 cut(s) 61, 403, 526
ApeKI GCWGC 3 cut(s) 9, 1028, 1245
ApoI RAATTY 3 cut(s) 258, 1103, 1273
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
AspLEI GCGC 2 cut(s) 9, 1446
AspS9I GGNCC 3 cut(s) 62, 248, 1406
AsuC2I CCSGG 3 cut(s) 60, 139, 941
AsuHPI GGTGA 1 cut(s) 1270
AsuNHI GCTAGC 1 cut(s) 26
AvaII GGWCC 2 cut(s) 248, 1406
BamHI GGATCC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 1349
Bbv12I GWGCWC 1 cut(s) 554
BbvCI CCTCAGC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 1040, 1232
BccI CCATC 4 cut(s) 27, 417, 1112, 1397
BceAI ACGGC 1 cut(s) 418
BcgI CGANNNNNNTGC 2 cut(s) 1391, 1425
BciVI GTATCC 1 cut(s) 577
BcnI CCSGG 3 cut(s) 60, 139, 941
BcoDI GTCTC 3 cut(s) 530, 1158, 1215
BfaI CTAG 5 cut(s) 27, 191, 573, 1284, 1452
BfmI CTRYAG 2 cut(s) 646, 1037
BfrI CTTAAG 1 cut(s) 578
BfuI GTATCC 1 cut(s) 577
BglII AGATCT 2 cut(s) 48, 874
BisI GCNGC 4 cut(s) 10, 1029, 1246, 1249
BlsI GCNGC 4 cut(s) 11, 1030, 1247, 1250
Bme1390I CCNGG 3 cut(s) 60, 139, 941
Bme18I GGWCC 2 cut(s) 248, 1406
BmgT120I GGNCC 3 cut(s) 62, 248, 1406
BmiI GGNNCC 2 cut(s) 143, 935
BmrFI CCNGG 3 cut(s) 60, 139, 941
BmrI ACTGGG 1 cut(s) 607
BmsI GCATC 5 cut(s) 196, 308, 498, 595, 1189
BmtI GCTAGC 1 cut(s) 30
BmuI ACTGGG 1 cut(s) 607
BpiI GAAGAC 1 cut(s) 1349
BplI GAGNNNNNCTC 4 cut(s) 98, 130, 319, 351
BpmI CTGGAG 1 cut(s) 377
Bpu10I CCTNAGC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 882
BpuMI CCSGG 3 cut(s) 60, 139, 941
BsaAI YACGTR 1 cut(s) 1308
BsaJI CCNNGG 3 cut(s) 428, 727, 799
BsaWI WCCGGW 1 cut(s) 96
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 485, 733
Bse1I ACTGG 3 cut(s) 84, 602, 935
Bse3DI GCAATG 4 cut(s) 302, 327, 608, 971
BseDI CCNNGG 3 cut(s) 428, 727, 799
BseGI GGATG 1 cut(s) 610
BseLI CCNNNNNNNGG 3 cut(s) 120, 485, 733
BseMI GCAATG 4 cut(s) 302, 327, 608, 971
BseMII CTCAG 6 cut(s) 28, 59, 567, 707, 1231, 1287
BseNI ACTGG 3 cut(s) 84, 602, 935
BseXI GCAGC 2 cut(s) 1040, 1232
BsgI GTGCAG 1 cut(s) 228
Bsh1236I CGCG 1 cut(s) 7
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 3 cut(s) 63, 405, 528
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 4 cut(s) 60, 97, 139, 940
BslI CCNNNNNNNGG 3 cut(s) 120, 485, 733
BsmAI GTCTC 3 cut(s) 530, 1158, 1215
BsmI GAATGC 1 cut(s) 1261
BsnI GGCC 3 cut(s) 63, 405, 528
Bsp1286I GDGCHC 1 cut(s) 554
Bsp1407I TGTACA 1 cut(s) 952
Bsp143I GATC 8 cut(s) 48, 90, 141, 416, 719, 874, 885, 1427
Bsp19I CCATGG 1 cut(s) 799
BspACI CCGC 4 cut(s) 443, 502, 1248, 1393
BspANI GGCC 3 cut(s) 63, 405, 528
BspCNI CTCAG 6 cut(s) 27, 60, 566, 708, 1230, 1288
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 143, 935
BspMAI CTGCAG 1 cut(s) 650
BspOI GCTAGC 1 cut(s) 30
BspPI GGATC 2 cut(s) 136, 149
BspTI CTTAAG 1 cut(s) 578
BsrDI GCAATG 4 cut(s) 302, 327, 608, 971
BsrGI TGTACA 1 cut(s) 952
BsrI ACTGG 3 cut(s) 84, 602, 935
BssECI CCNNGG 3 cut(s) 428, 727, 799
BssMI GATC 8 cut(s) 48, 90, 141, 416, 719, 874, 885, 1427
BssNAI GTATAC 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 428, 799
Bst1107I GTATAC 1 cut(s) 233
Bst4CI ACNGT 3 cut(s) 589, 839, 1077
Bst6I CTCTTC 3 cut(s) 123, 173, 273
BstAFI CTTAAG 1 cut(s) 578
BstAUI TGTACA 1 cut(s) 952
BstBAI YACGTR 1 cut(s) 1308
BstC8I GCNNGC 5 cut(s) 28, 162, 367, 703, 1362
BstDEI CTNAG 9 cut(s) 14, 68, 553, 716, 1185, 1217, 1296, 1373, 1419
BstDSI CCRYGG 2 cut(s) 727, 799
BstF5I GGATG 1 cut(s) 610
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 2 cut(s) 9, 1446
BstKTI GATC 8 cut(s) 51, 93, 144, 419, 722, 877, 888, 1430
BstMAI GTCTC 3 cut(s) 530, 1158, 1215
BstMBI GATC 8 cut(s) 48, 90, 141, 416, 719, 874, 885, 1427
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 4 cut(s) 15, 166, 802, 1361
BstNSI RCATGY 1 cut(s) 705
BstSCI CCNGG 3 cut(s) 58, 137, 939
BstSFI CTRYAG 2 cut(s) 646, 1037
BstSNI TACGTA 1 cut(s) 1308
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 2 cut(s) 1040, 1232
BstV2I GAAGAC 1 cut(s) 1349
BstX2I RGATCY 3 cut(s) 48, 141, 874
BstYI RGATCY 3 cut(s) 48, 141, 874
BstZ17I GTATAC 1 cut(s) 233
BsuI GTATCC 1 cut(s) 577
BsuRI GGCC 3 cut(s) 63, 405, 528
BtgI CCRYGG 2 cut(s) 727, 799
BtsCI GGATG 1 cut(s) 610
BtsI GCAGTG 1 cut(s) 643
BtsIMutI CAGTG 4 cut(s) 65, 91, 643, 1347
Cac8I GCNNGC 5 cut(s) 28, 162, 367, 703, 1362
CaiI CAGNNNCTG 1 cut(s) 1232
CfoI GCGC 2 cut(s) 9, 1446
Cfr13I GGNCC 3 cut(s) 62, 248, 1406
CseI GACGC 1 cut(s) 13
Csp6I GTAC 2 cut(s) 173, 953
CviAII CATG 6 cut(s) 55, 452, 702, 800, 956, 1242
CviQI GTAC 2 cut(s) 173, 953
DdeI CTNAG 9 cut(s) 14, 68, 553, 716, 1185, 1217, 1296, 1373, 1419
DpnI GATC 8 cut(s) 50, 92, 143, 418, 721, 876, 887, 1429
DpnII GATC 8 cut(s) 48, 90, 141, 416, 719, 874, 885, 1427
DraIII CACNNNGTG 1 cut(s) 728
Eam1104I CTCTTC 3 cut(s) 123, 173, 273
EarI CTCTTC 3 cut(s) 123, 173, 273
EciI GGCGGA 1 cut(s) 1408
Eco105I TACGTA 1 cut(s) 1308
Eco130I CCWWGG 2 cut(s) 428, 799
Eco32I GATATC 1 cut(s) 999
Eco47I GGWCC 2 cut(s) 248, 1406
Eco57I CTGAAG 5 cut(s) 254, 395, 743, 833, 1371
EcoRV GATATC 1 cut(s) 999
EcoT14I CCWWGG 2 cut(s) 428, 799
EcoT22I ATGCAT 1 cut(s) 610
ErhI CCWWGG 2 cut(s) 428, 799
FaeI CATG 6 cut(s) 58, 455, 705, 803, 959, 1245
FatI CATG 6 cut(s) 54, 451, 701, 799, 955, 1241
FauI CCCGC 1 cut(s) 495
FauNDI CATATG 1 cut(s) 339
FblI GTMKAC 2 cut(s) 232, 1008
Fnu4HI GCNGC 4 cut(s) 10, 1029, 1246, 1249
FokI GGATG 1 cut(s) 617
Fsp4HI GCNGC 4 cut(s) 10, 1029, 1246, 1249
FspBI CTAG 5 cut(s) 27, 191, 573, 1284, 1452
GlaI GCGC 2 cut(s) 8, 1445
GluI GCNGC 4 cut(s) 10, 1029, 1246, 1249
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 3 cut(s) 63, 405, 528
HapII CCGG 4 cut(s) 60, 97, 139, 940
HgaI GACGC 1 cut(s) 13
HhaI GCGC 2 cut(s) 9, 1446
Hin1II CATG 6 cut(s) 58, 455, 705, 803, 959, 1245
Hin6I GCGC 2 cut(s) 7, 1444
HinP1I GCGC 2 cut(s) 7, 1444
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 1187
HinfI GANTC 7 cut(s) 107, 128, 265, 334, 628, 853, 1238
HpaII CCGG 4 cut(s) 60, 97, 139, 940
HphI GGTGA 1 cut(s) 1270
Hpy166II GTNNAC 6 cut(s) 201, 233, 400, 928, 953, 1009
Hpy188I TCNGA 9 cut(s) 43, 127, 556, 717, 762, 852, 870, 885, 1220
Hpy188III TCNNGA 4 cut(s) 420, 543, 593, 1016
Hpy8I GTNNAC 6 cut(s) 201, 233, 400, 928, 953, 1009
HpyAV CCTTC 5 cut(s) 272, 489, 540, 775, 1012
HpyCH4III ACNGT 3 cut(s) 589, 839, 1077
HpyCH4IV ACGT 3 cut(s) 1149, 1307, 1326
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 166, 802, 1361
HpyF3I CTNAG 9 cut(s) 14, 68, 553, 716, 1185, 1217, 1296, 1373, 1419
HpySE526I ACGT 3 cut(s) 1149, 1307, 1326
Hsp92II CATG 6 cut(s) 58, 455, 705, 803, 959, 1245
HspAI GCGC 2 cut(s) 7, 1444
Kzo9I GATC 8 cut(s) 48, 90, 141, 416, 719, 874, 885, 1427
LmnI GCTCC 3 cut(s) 569, 801, 973
Lsp1109I GCAGC 2 cut(s) 1040, 1232
LweI GCATC 5 cut(s) 196, 308, 498, 595, 1189
MaeI CTAG 5 cut(s) 27, 191, 573, 1284, 1452
MaeII ACGT 3 cut(s) 1149, 1307, 1326
MaeIII GTNAC 3 cut(s) 455, 1318, 1330
MalI GATC 8 cut(s) 50, 92, 143, 418, 721, 876, 887, 1429
MboI GATC 8 cut(s) 48, 90, 141, 416, 719, 874, 885, 1427
MboII GAAGA 9 cut(s) 110, 190, 260, 404, 445, 749, 839, 1354, 1408
MfeI CAATTG 3 cut(s) 1055, 1086, 1159
MflI RGATCY 3 cut(s) 48, 141, 874
MhlI GDGCHC 1 cut(s) 554
MluCI AATT 7 cut(s) 258, 747, 1055, 1086, 1103, 1159, 1273
MlyI GAGTC 3 cut(s) 116, 259, 328
Mph1103I ATGCAT 1 cut(s) 610
MseI TTAA 5 cut(s) 579, 753, 963, 1146, 1386
MslI CAYNNNNRTG 1 cut(s) 700
MspCI CTTAAG 1 cut(s) 578
MspI CCGG 4 cut(s) 60, 97, 139, 940
MspR9I CCNGG 3 cut(s) 60, 139, 941
MunI CAATTG 3 cut(s) 1055, 1086, 1159
Mva1269I GAATGC 1 cut(s) 1261
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 4 cut(s) 15, 166, 802, 1361
NciI CCSGG 3 cut(s) 60, 139, 941
NcoI CCATGG 1 cut(s) 799
NdeI CATATG 1 cut(s) 339
NdeII GATC 8 cut(s) 48, 90, 141, 416, 719, 874, 885, 1427
NheI GCTAGC 1 cut(s) 26
NlaIII CATG 6 cut(s) 58, 455, 705, 803, 959, 1245
NlaIV GGNNCC 2 cut(s) 143, 935
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 1330
NsiI ATGCAT 1 cut(s) 610
NspI RCATGY 1 cut(s) 705
PaeI GCATGC 1 cut(s) 705
PctI GAATGC 1 cut(s) 1261
PfeI GAWTC 4 cut(s) 128, 628, 853, 1238
PkrI GCNGC 4 cut(s) 11, 1030, 1247, 1250
PleI GAGTC 3 cut(s) 115, 259, 328
PpsI GAGTC 3 cut(s) 115, 259, 328
Ppu21I YACGTR 1 cut(s) 1308
PsiI TTATAA 1 cut(s) 1023
Psp1406I AACGTT 1 cut(s) 1326
PspN4I GGNNCC 2 cut(s) 143, 935
PspPI GGNCC 3 cut(s) 62, 248, 1406
PstI CTGCAG 1 cut(s) 650
PstNI CAGNNNCTG 1 cut(s) 1232
PsuI RGATCY 3 cut(s) 48, 141, 874
RsaI GTAC 2 cut(s) 174, 954
RsaNI GTAC 2 cut(s) 173, 953
RseI CAYNNNNRTG 1 cut(s) 700
SaqAI TTAA 5 cut(s) 579, 753, 963, 1146, 1386
SatI GCNGC 4 cut(s) 10, 1029, 1246, 1249
Sau3AI GATC 8 cut(s) 48, 90, 141, 416, 719, 874, 885, 1427
Sau96I GGNCC 3 cut(s) 62, 248, 1406
SchI GAGTC 3 cut(s) 116, 259, 328
ScrFI CCNGG 3 cut(s) 60, 139, 941
SduI GDGCHC 1 cut(s) 554
SfaNI GCATC 5 cut(s) 196, 308, 498, 595, 1189
SfcI CTRYAG 2 cut(s) 646, 1037
SinI GGWCC 2 cut(s) 248, 1406
SmiMI CAYNNNNRTG 1 cut(s) 700
SmlI CTYRAG 2 cut(s) 578, 897
SmoI CTYRAG 2 cut(s) 578, 897
SnaBI TACGTA 1 cut(s) 1308
SphI GCATGC 1 cut(s) 705
Sse9I AATT 7 cut(s) 258, 747, 1055, 1086, 1103, 1159, 1273
SsiI CCGC 4 cut(s) 443, 502, 1248, 1393
SspMI CTAG 5 cut(s) 27, 191, 573, 1284, 1452
StyD4I CCNGG 3 cut(s) 58, 137, 939
StyI CCWWGG 2 cut(s) 428, 799
TaaI ACNGT 3 cut(s) 589, 839, 1077
TaiI ACGT 3 cut(s) 1152, 1310, 1329
TaqI TCGA 4 cut(s) 93, 244, 415, 492
TaqII GACCGA 1 cut(s) 111
TasI AATT 7 cut(s) 258, 747, 1055, 1086, 1103, 1159, 1273
TatI WGTACW 1 cut(s) 952
TauI GCSGC 1 cut(s) 1251
TfiI GAWTC 4 cut(s) 128, 628, 853, 1238
Tru1I TTAA 5 cut(s) 579, 753, 963, 1146, 1386
Tru9I TTAA 5 cut(s) 579, 753, 963, 1146, 1386
TscAI CASTG 4 cut(s) 72, 91, 650, 1354
TseFI GTSAC 1 cut(s) 1330
TseI GCWGC 3 cut(s) 9, 1028, 1245
Tsp45I GTSAC 1 cut(s) 1330
TspDTI ATGAA 6 cut(s) 302, 641, 930, 970, 1102, 1251
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 4 cut(s) 72, 91, 650, 1354
Vha464I CTTAAG 1 cut(s) 578
VpaK11BI GGWCC 2 cut(s) 248, 1406
XapI RAATTY 3 cut(s) 258, 1103, 1273
XceI RCATGY 1 cut(s) 705
XcmI CCANNNNNNNNNTGG 2 cut(s) 258, 416
XmiI GTMKAC 2 cut(s) 232, 1008
XspI CTAG 5 cut(s) 27, 191, 573, 1284, 1452
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.