Rw6G032500

N-glycosylase DNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015250
Physical Location & Seq
Reverse (-)
56650120 .. 56652212
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032500.1

Sequence Viewer

Length: 768 bp
ATGGGTATTTCCCTGGCCGGAATTTGGGAGGTTTTCTCGGCTTCCGATTCCAGGTTTGCCGAGCTCGCCACCCATTTGGGCGGCGCTAGAGTTCTCAGGCAAGACCCACTTGAGTGTTTGATTCAGTTCCTGTGTTCTTCAAATAACAACATTCAGAGAATTACCAAAATGGTGGACTTTGTGTCTTCACTGGGGAACCATTTGGGGTCTGCAGGAGGTTTTGAGTTCCATGAATTCCCATCCTTGGATCGCTTGGCAATGGTGTCGGAACAAGAGCTCAGAGAGGCCGGTTTCGGTTATAGGGCTAAGTACTTAACTGGCACTGTGAAGGCTTTGCAGTCGAAACCTGGTGGAGGTGAAGAGTGGCTTTTGTCTCTTCGGAAACTTGAACTCGAAGAGGTGATTGACGCTCTTACCACATTGCCTGGAGTTGGTCCCAAGGTGGCAGCATGTATAGCTCTTTTTTCGCTTGATCAACACAATGCCATTCCTGTTGATACACACGTGTGGAAGATTGCTACAAGATACCTCATACCTGAGCTAGCAGGGGCTCGTCTGACACCGAAGCTCTGCGGCCGTGTGGCGGAGGCATTTGTGAGTAAATATGGGAAATATGCTGGATGGGCTCAAACTCTGCTGTTTGTTGCTGAATTGCCTTCACAAAAAGCCCTCCTACCACCACATTTTTCAAATGCCAAAGAGAGTAAATCTGCAAAAGGAAAGGGCCGTGGAGTGCAGACTGTAGCGGATGCCTCAAACAGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000702 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0006139 GO:0006220 GO:0006244 GO:0006259 GO:0006281 GO:0006282 GO:0006284 GO:0006285 GO:0006289 GO:0006304 GO:0006355 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0008534 GO:0009056 GO:0009058 GO:0009117 GO:0009166 GO:0009219 GO:0009223 GO:0009262 GO:0009264 GO:0009314 GO:0009394 GO:0009628 GO:0009889 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010605 GO:0016363 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016798 GO:0016799 GO:0018130 GO:0019104 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0019637 GO:0019692 GO:0031323 GO:0031324 GO:0031326 GO:0031974 GO:0031981 GO:0032356 GO:0032357 GO:0032991 GO:0033554 GO:0033683 GO:0034399 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045007 GO:0045008 GO:0045738 GO:0045934 GO:0046386 GO:0046434 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051252 GO:0051716 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0072527 GO:0072529 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901135 GO:1901136 GO:1901291 GO:1901292 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1903506 GO:2000112 GO:2000779 GO:2000780 GO:2001020 GO:2001021 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
HhH-GPD OGG_N

Protein Analysis

255

Amino Acids

27.62

Weight (kDa)

7.7

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGG_N PF07934 3 - 40 1e-05 8-oxoguanine DNA glycosylase, N-terminal domain
HhH-GPD PF00730 42 - 178 6.3e-10 HhH-GPD superfamily base excision DNA repair protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21710
fragaria_vesca FvH4_2g27490
prunus_persica Prupe.1G492700_v2.0.a1
pyrus_communis pycom15g30910
rosa_chinensis RchiOBHm_Chr6g0296321
rosa_laevigata RLG00000011706
rosa_multiflora Rmu_co8447813.1_g000001 Rmu_sc0001478.1_g000002
rosa_roxburghii Rroxscaffold_7G00171630 Rroxscaffold_7G00178450
rosa_rugosa Rorug06G0261100
rosa_samantha Rh6AG372800 Rh6BG380900 Rh6CG386100 Rh6DG373600
rosa_wichuraiana Rw6G032500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 81, 573, 584, 746
AclWI GGATC 1 cut(s) 255
AcoI YGGCCR 2 cut(s) 15, 574
AcsI RAATTY 2 cut(s) 21, 233
AcvI CACGTG 1 cut(s) 505
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 6 cut(s) 24, 51, 244, 353, 431, 583
AflIII ACRYGT 2 cut(s) 502, 504
AgsI TTSAA 3 cut(s) 141, 389, 690
AhdI GACNNNNNGTC 1 cut(s) 181
AjnI CCWGG 4 cut(s) 12, 50, 346, 424
AleI CACNNNNGTG 2 cut(s) 112, 505
AluBI AGCT 5 cut(s) 64, 277, 458, 541, 568
AluI AGCT 5 cut(s) 64, 277, 458, 541, 568
Alw21I GWGCWC 2 cut(s) 66, 279
Alw26I GTCTC 1 cut(s) 378
AlwI GGATC 1 cut(s) 255
AlwNI CAGNNNCTG 1 cut(s) 130
AoxI GGCC 4 cut(s) 15, 285, 574, 724
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 2 cut(s) 21, 233
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 434, 724
AsuHPI GGTGA 2 cut(s) 368, 412
AsuNHI GCTAGC 1 cut(s) 541
AvaII GGWCC 1 cut(s) 434
BaeI ACNNNNGTAYC 2 cut(s) 489, 522
BanII GRGCYC 4 cut(s) 66, 279, 553, 628
BbrPI CACGTG 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 2 cut(s) 66, 279
BbvI GCAGC 1 cut(s) 458
BccI CCATC 2 cut(s) 247, 615
BceAI ACGGC 2 cut(s) 561, 711
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BciT130I CCWGG 4 cut(s) 14, 52, 348, 426
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 378
BfaI CTAG 2 cut(s) 87, 542
BfmI CTRYAG 2 cut(s) 210, 741
BfoI RGCGCY 1 cut(s) 87
BisI GCNGC 3 cut(s) 82, 447, 574
BlsI GCNGC 3 cut(s) 83, 448, 575
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 4 cut(s) 14, 52, 348, 426
Bme18I GGWCC 1 cut(s) 434
BmeRI GACNNNNNGTC 1 cut(s) 181
BmgT120I GGNCC 2 cut(s) 434, 724
BmiI GGNNCC 2 cut(s) 197, 436
BmrFI CCNGG 4 cut(s) 14, 52, 348, 426
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 739
BmtI GCTAGC 1 cut(s) 545
BmuI ACTGGG 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 177
BplI GAGNNNNNCTC 2 cut(s) 20, 52
BpmI CTGGAG 1 cut(s) 447
Bpu10I CCTNAGC 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 505
BsaJI CCNNGG 4 cut(s) 12, 243, 438, 727
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 51, 244, 353, 431, 583
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 195, 322
Bse3DI GCAATG 2 cut(s) 264, 419
BseBI CCWGG 4 cut(s) 14, 52, 348, 426
BseDI CCNNGG 4 cut(s) 12, 243, 438, 727
BseGI GGATG 3 cut(s) 239, 626, 754
BseLI CCNNNNNNNGG 6 cut(s) 24, 51, 244, 353, 431, 583
BseMI GCAATG 2 cut(s) 264, 419
BseMII CTCAG 3 cut(s) 109, 292, 528
BseNI ACTGG 2 cut(s) 195, 322
BseX3I CGGCCG 1 cut(s) 574
BseXI GCAGC 1 cut(s) 458
BsgI GTGCAG 1 cut(s) 755
Bsh1285I CGRYCG 1 cut(s) 577
BshFI GGCC 4 cut(s) 17, 287, 576, 726
BsiEI CGRYCG 1 cut(s) 577
BsiHKAI GWGCWC 2 cut(s) 66, 279
BsiSI CCGG 2 cut(s) 18, 288
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 6 cut(s) 24, 51, 244, 353, 431, 583
BsmAI GTCTC 1 cut(s) 378
BsmFI GGGAC 1 cut(s) 420
BsnI GGCC 4 cut(s) 17, 287, 576, 726
Bsp1286I GDGCHC 4 cut(s) 66, 279, 553, 628
Bsp143I GATC 2 cut(s) 247, 472
BspACI CCGC 4 cut(s) 81, 573, 584, 746
BspANI GGCC 4 cut(s) 17, 287, 576, 726
BspCNI CTCAG 3 cut(s) 108, 291, 529
BspLI GGNNCC 2 cut(s) 197, 436
BspMAI CTGCAG 1 cut(s) 214
BspOI GCTAGC 1 cut(s) 545
BspPI GGATC 1 cut(s) 255
BsrDI GCAATG 2 cut(s) 264, 419
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 2 cut(s) 195, 322
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 4 cut(s) 12, 243, 438, 727
BssMI GATC 2 cut(s) 247, 472
BssT1I CCWWGG 2 cut(s) 243, 438
Bst2UI CCWGG 4 cut(s) 14, 52, 348, 426
Bst4CI ACNGT 3 cut(s) 325, 742, 761
Bst6I CTCTTC 3 cut(s) 354, 381, 390
BstBAI YACGTR 1 cut(s) 505
BstC8I GCNNGC 2 cut(s) 66, 543
BstDEI CTNAG 4 cut(s) 95, 278, 306, 537
BstDSI CCRYGG 1 cut(s) 727
BstENI CCTNNNNNAGG 1 cut(s) 351
BstF5I GGATG 3 cut(s) 239, 626, 754
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 2 cut(s) 250, 475
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 2 cut(s) 247, 472
BstMCI CGRYCG 1 cut(s) 577
BstMWI GCNNNNNNNGC 3 cut(s) 65, 455, 623
BstNI CCWGG 4 cut(s) 14, 52, 348, 426
BstNSI RCATGY 1 cut(s) 453
BstSCI CCNGG 4 cut(s) 12, 50, 346, 424
BstSFI CTRYAG 2 cut(s) 210, 741
BstV1I GCAGC 1 cut(s) 458
BstV2I GAAGAC 1 cut(s) 177
BstXI CCANNNNNNTGG 2 cut(s) 76, 172
BstZI CGGCCG 1 cut(s) 574
BsuRI GGCC 4 cut(s) 17, 287, 576, 726
BtgI CCRYGG 1 cut(s) 727
BtsCI GGATG 3 cut(s) 239, 626, 754
BtsIMutI CAGTG 3 cut(s) 188, 321, 766
Cac8I GCNNGC 2 cut(s) 66, 543
CaiI CAGNNNCTG 1 cut(s) 130
CfoI GCGC 1 cut(s) 86
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 434, 724
CseI GACGC 1 cut(s) 416
CsiI ACCWGGT 1 cut(s) 346
Csp6I GTAC 1 cut(s) 310
CviAII CATG 2 cut(s) 230, 450
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 4 cut(s) 95, 278, 306, 537
DpnI GATC 2 cut(s) 249, 474
DpnII GATC 2 cut(s) 247, 472
DriI GACNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 2 cut(s) 15, 574
EagI CGGCCG 1 cut(s) 574
Eam1104I CTCTTC 3 cut(s) 354, 381, 390
Eam1105I GACNNNNNGTC 1 cut(s) 181
EarI CTCTTC 3 cut(s) 354, 381, 390
EciI GGCGGA 1 cut(s) 599
Ecl136II GAGCTC 2 cut(s) 64, 277
EclXI CGGCCG 1 cut(s) 574
Eco130I CCWWGG 2 cut(s) 243, 438
Eco24I GRGCYC 4 cut(s) 66, 279, 553, 628
Eco47I GGWCC 1 cut(s) 434
Eco52I CGGCCG 1 cut(s) 574
Eco53kI GAGCTC 2 cut(s) 64, 277
Eco72I CACGTG 1 cut(s) 505
EcoICRI GAGCTC 2 cut(s) 64, 277
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 4 cut(s) 12, 50, 346, 424
EcoT14I CCWWGG 2 cut(s) 243, 438
EcoT38I GRGCYC 4 cut(s) 66, 279, 553, 628
ErhI CCWWGG 2 cut(s) 243, 438
FaeI CATG 2 cut(s) 233, 453
FaiI YATR 7 cut(s) 231, 300, 451, 455, 533, 606, 615
FalI AAGNNNNNCTT 4 cut(s) 93, 125, 351, 383
FaqI GGGAC 1 cut(s) 420
FatI CATG 2 cut(s) 229, 449
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 3 cut(s) 82, 447, 574
FokI GGATG 3 cut(s) 226, 633, 761
FriOI GRGCYC 4 cut(s) 66, 279, 553, 628
Fsp4HI GCNGC 3 cut(s) 82, 447, 574
FspBI CTAG 2 cut(s) 87, 542
GlaI GCGC 1 cut(s) 85
GluI GCNGC 3 cut(s) 82, 447, 574
GsuI CTGGAG 1 cut(s) 447
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 4 cut(s) 17, 287, 576, 726
HapII CCGG 2 cut(s) 18, 288
HgaI GACGC 1 cut(s) 416
HhaI GCGC 1 cut(s) 86
Hin1II CATG 2 cut(s) 233, 453
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HinfI GANTC 2 cut(s) 47, 121
HpaII CCGG 2 cut(s) 18, 288
HphI GGTGA 2 cut(s) 368, 412
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 6 cut(s) 46, 156, 268, 281, 381, 558
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 2 cut(s) 322, 666
HpyCH4III ACNGT 3 cut(s) 325, 742, 761
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 4 cut(s) 212, 337, 713, 736
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 455, 623
HpyF3I CTNAG 4 cut(s) 95, 278, 306, 537
HpySE526I ACGT 1 cut(s) 504
Hsp92II CATG 2 cut(s) 233, 453
HspAI GCGC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 2 cut(s) 247, 472
Lsp1109I GCAGC 1 cut(s) 458
LweI GCATC 1 cut(s) 739
MabI ACCWGGT 1 cut(s) 346
MaeI CTAG 2 cut(s) 87, 542
MaeII ACGT 1 cut(s) 504
MalI GATC 2 cut(s) 249, 474
MboI GATC 2 cut(s) 247, 472
MboII GAAGA 6 cut(s) 129, 177, 368, 371, 407, 523
MhlI GDGCHC 4 cut(s) 66, 279, 553, 628
MluCI AATT 4 cut(s) 21, 159, 233, 650
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 9 cut(s) 22, 209, 277, 347, 391, 539, 580, 680, 763
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 2 cut(s) 112, 505
MspI CCGG 2 cut(s) 18, 288
MspR9I CCNGG 4 cut(s) 14, 52, 348, 426
MvaI CCWGG 4 cut(s) 14, 52, 348, 426
MwoI GCNNNNNNNGC 3 cut(s) 65, 455, 623
NdeII GATC 2 cut(s) 247, 472
NheI GCTAGC 1 cut(s) 541
NlaIII CATG 2 cut(s) 233, 453
NlaIV GGNNCC 2 cut(s) 197, 436
NmeAIII GCCGAG 2 cut(s) 17, 85
NspI RCATGY 1 cut(s) 453
OliI CACNNNNGTG 2 cut(s) 112, 505
PfeI GAWTC 2 cut(s) 47, 121
PkrI GCNGC 3 cut(s) 83, 448, 575
PmaCI CACGTG 1 cut(s) 505
PmlI CACGTG 1 cut(s) 505
Ppu21I YACGTR 1 cut(s) 505
Psp124BI GAGCTC 2 cut(s) 66, 279
Psp6I CCWGG 4 cut(s) 12, 50, 346, 424
PspCI CACGTG 1 cut(s) 505
PspGI CCWGG 4 cut(s) 12, 50, 346, 424
PspN4I GGNNCC 2 cut(s) 197, 436
PspPI GGNCC 2 cut(s) 434, 724
PstI CTGCAG 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 130
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 2 cut(s) 112, 505
SacI GAGCTC 2 cut(s) 66, 279
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 3 cut(s) 82, 447, 574
Sau3AI GATC 2 cut(s) 247, 472
Sau96I GGNCC 2 cut(s) 434, 724
ScaI AGTACT 1 cut(s) 311
ScrFI CCNGG 4 cut(s) 14, 52, 348, 426
SduI GDGCHC 4 cut(s) 66, 279, 553, 628
SexAI ACCWGGT 1 cut(s) 346
SfaNI GCATC 1 cut(s) 739
SfcI CTRYAG 2 cut(s) 210, 741
SinI GGWCC 1 cut(s) 434
SmiMI CAYNNNNRTG 2 cut(s) 112, 505
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 4 cut(s) 21, 159, 233, 650
SsiI CCGC 4 cut(s) 81, 573, 584, 746
SspMI CTAG 2 cut(s) 87, 542
SstI GAGCTC 2 cut(s) 66, 279
StyD4I CCNGG 4 cut(s) 12, 50, 346, 424
StyI CCWWGG 2 cut(s) 243, 438
TaaI ACNGT 3 cut(s) 325, 742, 761
TaiI ACGT 1 cut(s) 507
TaqI TCGA 2 cut(s) 341, 393
TasI AATT 4 cut(s) 21, 159, 233, 650
TatI WGTACW 1 cut(s) 309
TauI GCSGC 2 cut(s) 84, 576
TfiI GAWTC 2 cut(s) 47, 121
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 3 cut(s) 195, 328, 766
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 3 cut(s) 195, 328, 766
VpaK11BI GGWCC 1 cut(s) 434
XagI CCTNNNNNAGG 1 cut(s) 351
XapI RAATTY 2 cut(s) 21, 233
XceI RCATGY 1 cut(s) 453
XspI CTAG 2 cut(s) 87, 542
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.