Rw6G035290

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0018390
Physical Location & Seq
Reverse (-)
59857901 .. 59859271
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035290.1

Sequence Viewer

Length: 1080 bp
ATGGCAGAGGGTGAGGGCGATGACCTTGTAGATGATGTTGTAGAAATAATCATACTCAAGTTGCCCTTGAAATCTATACTGAAATTTCGATGCGTCTCAAAAAAATGGAGCTGTAGAATCTCTGACTATAGATTCTCCCGAAGGCACTATAAATTAGCGCGTTGCCAGAAAACTGTGAATCACAGGCTCATCATCATCTCCTCATCTCAAATGGAATCCTTGGACCTCCAGGCGAAGTCATCATCAATTAGAAAGCTTACCTGCCCATTCTTGGAACTCGAGCAATGGCGTAGTGGTTTGGTATGTGCAAGTTTCGATCCTCATAAAAACTTCATCTTATGGAATCCATCAACCACTTTCTTCAGGAAATTACCCGACCCAGATCTTGGTGGTTATTTTTCAGAGGAAGAAGCAGAAGAAGTTGAGTTTAGTGTTTATCACTACCGCTTTGGTTGTGTGTCGGCTACTGACGACTATAAACTTTTGATAGGGTTTCGGATTAACTCTGATTTCGAGGAGCTCTTAATATTCTCATGGAAGAATCAAATTTGGAGAGCCATTTCCGGTGCGCCAAAGTGGATCAACCGCATAACCCAAAGTCAGGGGGCTCTTTCAAATGAAAAACTTCACTGGCACTTCGATCACAATAATGGGCTCTATGAATCACATGAGGAGGAAGATTGTGTCCTTACTTTCTATGCTAATCTTTTGGGGGTCTCCTGTGAAGGATGCCTATACGTGTTGCATCATATGGAGGACTGGTTCTCTAGTATGACATCTGCTAGTATTTGGTACATGAAAGAATATGGGGTGACTGACTCGTGGAGTAAGCTCTTTGATTTAAAGATTTCCCATGAGCCTCTTGAGCCGGTAACTTTTCTCGGTCCAATCTTTTTCATGGAGAAATCATCAGTCTATGGCTATGGGAACAAGTTCACAGAGATCAAGTATCAGCATCAGCCTCAGCTTCAGCCTCAGCCTCACATAGATTTGCTGAAGCGAGAAAACCTACTTGACATGATTGTATGTGAAGAGACTCTGGTCTGTCTCGATGATGCTGATGAAGAAGAGGTTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

359

Amino Acids

41.94

Weight (kDa)

5.23

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 8 - 45 2.7e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g40801
rosa_chinensis RchiOBHm_Chr6g0299781
rosa_laevigata RLG00000011388 RLG00000011389
rosa_multiflora Rmu_ssc0000158.1_g000002 Rmu_ssc0000158.1_g000003
rosa_roxburghii Rroxscaffold_7G00168380
rosa_rugosa Rorug06G0292200
rosa_samantha Rh6CG417800
rosa_wichuraiana Rw6G035290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 386
AccII CGCG 1 cut(s) 160
AciI CCGC 2 cut(s) 445, 586
AclWI GGATC 2 cut(s) 311, 587
AcsI RAATTY 2 cut(s) 83, 546
AcuI CTGAAG 3 cut(s) 346, 953, 1016
AfaI GTAC 1 cut(s) 794
AfiI CCNNNNNNNGG 3 cut(s) 271, 386, 601
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 2 cut(s) 70, 615
AjnI CCWGG 1 cut(s) 228
AloI GAACNNNNNNTCC 2 cut(s) 746, 778
AluBI AGCT 5 cut(s) 111, 256, 520, 832, 967
AluI AGCT 5 cut(s) 111, 256, 520, 832, 967
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 4 cut(s) 100, 721, 1028, 1052
AlwI GGATC 2 cut(s) 311, 587
Ama87I CYCGRG 1 cut(s) 278
ApoI RAATTY 2 cut(s) 83, 546
Asp700I GAANNNNTTC 2 cut(s) 624, 932
AspLEI GCGC 2 cut(s) 160, 571
AspS9I GGNCC 2 cut(s) 223, 884
AsuHPI GGTGA 2 cut(s) 23, 823
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 2 cut(s) 223, 884
BanII GRGCYC 3 cut(s) 522, 610, 657
BauI CACGAG 1 cut(s) 820
Bbv12I GWGCWC 1 cut(s) 522
BbvCI CCTCAGC 2 cut(s) 963, 975
BccI CCATC 1 cut(s) 355
BciT130I CCWGG 1 cut(s) 230
BcoDI GTCTC 4 cut(s) 100, 721, 1028, 1052
BfaI CTAG 2 cut(s) 768, 783
BfmI CTRYAG 2 cut(s) 112, 127
BfuAI ACCTGC 1 cut(s) 269
BglII AGATCT 1 cut(s) 382
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 2 cut(s) 223, 884
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 2 cut(s) 223, 884
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 5 cut(s) 80, 719, 754, 964, 1045
BoxI GACNNNNGTC 1 cut(s) 1040
BpmI CTGGAG 1 cut(s) 212
Bpu10I CCTNAGC 2 cut(s) 963, 975
BpuEI CTTGAG 2 cut(s) 41, 884
BsaAI YACGTR 1 cut(s) 739
BsaI GGTCTC 1 cut(s) 721
BsaJI CCNNGG 1 cut(s) 219
BsaWI WCCGGW 1 cut(s) 563
BsaXI ACNNNNNCTCC 2 cut(s) 746, 776
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 386, 601
Bse118I RCCGGY 1 cut(s) 868
Bse1I ACTGG 2 cut(s) 635, 764
Bse3DI GCAATG 1 cut(s) 290
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 1 cut(s) 734
BseLI CCNNNNNNNGG 3 cut(s) 271, 386, 601
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 2 cut(s) 977, 989
BseNI ACTGG 2 cut(s) 635, 764
BseRI GAGGAG 3 cut(s) 190, 530, 686
Bsh1236I CGCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 522
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 2 cut(s) 564, 869
BslI CCNNNNNNNGG 3 cut(s) 271, 386, 601
BsmAI GTCTC 4 cut(s) 100, 721, 1028, 1052
BsmBI CGTCTC 1 cut(s) 100
Bso31I GGTCTC 1 cut(s) 721
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 3 cut(s) 522, 610, 657
Bsp143I GATC 5 cut(s) 316, 382, 579, 640, 942
BspACI CCGC 2 cut(s) 445, 586
BspCNI CTCAG 2 cut(s) 976, 988
BspFNI CGCG 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 2 cut(s) 311, 587
BspTNI GGTCTC 1 cut(s) 721
BsrDI GCAATG 1 cut(s) 290
BsrFI RCCGGY 1 cut(s) 868
BsrI ACTGG 2 cut(s) 635, 764
BssAI RCCGGY 1 cut(s) 868
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 5 cut(s) 316, 382, 579, 640, 942
BssSI CACGAG 1 cut(s) 820
BssT1I CCWWGG 1 cut(s) 219
Bst2BI CACGAG 1 cut(s) 820
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 175
Bst6I CTCTTC 2 cut(s) 1026, 1062
BstBAI YACGTR 1 cut(s) 739
BstDEI CTNAG 2 cut(s) 963, 975
BstF5I GGATG 1 cut(s) 734
BstFNI CGCG 1 cut(s) 160
BstHHI GCGC 2 cut(s) 160, 571
BstKTI GATC 5 cut(s) 319, 385, 582, 643, 945
BstMAI GTCTC 4 cut(s) 100, 721, 1028, 1052
BstMBI GATC 5 cut(s) 316, 382, 579, 640, 942
BstMWI GCNNNNNNNGC 1 cut(s) 865
BstNI CCWGG 1 cut(s) 230
BstPAI GACNNNNGTC 1 cut(s) 1040
BstSCI CCNGG 1 cut(s) 228
BstSFI CTRYAG 2 cut(s) 112, 127
BstUI CGCG 1 cut(s) 160
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BtgZI GCGATG 1 cut(s) 33
BtsCI GGATG 1 cut(s) 734
BtsIMutI CAGTG 1 cut(s) 628
BveI ACCTGC 1 cut(s) 269
CfoI GCGC 2 cut(s) 160, 571
Cfr10I RCCGGY 1 cut(s) 868
Cfr13I GGNCC 2 cut(s) 223, 884
CseI GACGC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 793
CviAII CATG 6 cut(s) 534, 668, 796, 854, 898, 1018
CviQI GTAC 1 cut(s) 793
DdeI CTNAG 2 cut(s) 963, 975
DpnI GATC 5 cut(s) 318, 384, 581, 642, 944
DpnII GATC 5 cut(s) 316, 382, 579, 640, 942
DraI TTTAAA 1 cut(s) 843
Eam1104I CTCTTC 2 cut(s) 1026, 1062
EarI CTCTTC 2 cut(s) 1026, 1062
Ecl136II GAGCTC 1 cut(s) 520
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 3 cut(s) 522, 610, 657
Eco31I GGTCTC 1 cut(s) 721
Eco47I GGWCC 2 cut(s) 223, 884
Eco53kI GAGCTC 1 cut(s) 520
Eco57I CTGAAG 3 cut(s) 346, 953, 1016
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 520
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 3 cut(s) 522, 610, 657
ErhI CCWWGG 1 cut(s) 219
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 6 cut(s) 537, 671, 799, 857, 901, 1021
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FatI CATG 6 cut(s) 533, 667, 795, 853, 897, 1017
FauNDI CATATG 1 cut(s) 750
FokI GGATG 1 cut(s) 741
FriOI GRGCYC 3 cut(s) 522, 610, 657
FspBI CTAG 2 cut(s) 768, 783
GlaI GCGC 2 cut(s) 159, 570
GsuI CTGGAG 1 cut(s) 212
HapII CCGG 2 cut(s) 564, 869
HgaI GACGC 1 cut(s) 82
HhaI GCGC 2 cut(s) 160, 571
Hin1II CATG 6 cut(s) 537, 671, 799, 857, 901, 1021
Hin6I GCGC 2 cut(s) 158, 569
HinP1I GCGC 2 cut(s) 158, 569
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 9 cut(s) 117, 132, 178, 215, 343, 541, 662, 818, 1036
HpaI GTTAAC 1 cut(s) 1075
HpaII CCGG 2 cut(s) 564, 869
HphI GGTGA 2 cut(s) 23, 823
Hpy166II GTNNAC 2 cut(s) 936, 1075
Hpy188I TCNGA 4 cut(s) 124, 403, 498, 508
Hpy188III TCNNGA 4 cut(s) 138, 364, 863, 1049
Hpy8I GTNNAC 2 cut(s) 936, 1075
HpyAV CCTTC 2 cut(s) 135, 719
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 1 cut(s) 738
HpyCH4V TGCA 2 cut(s) 308, 745
HpyF10VI GCNNNNNNNGC 1 cut(s) 865
HpyF3I CTNAG 2 cut(s) 963, 975
HpySE526I ACGT 1 cut(s) 738
Hsp92II CATG 6 cut(s) 537, 671, 799, 857, 901, 1021
HspAI GCGC 2 cut(s) 158, 569
KspAI GTTAAC 1 cut(s) 1075
Kzo9I GATC 5 cut(s) 316, 382, 579, 640, 942
LmnI GCTCC 2 cut(s) 108, 517
LweI GCATC 5 cut(s) 80, 719, 754, 964, 1045
MaeI CTAG 2 cut(s) 768, 783
MaeII ACGT 1 cut(s) 738
MaeIII GTNAC 2 cut(s) 811, 871
MalI GATC 5 cut(s) 318, 384, 581, 642, 944
MboI GATC 5 cut(s) 316, 382, 579, 640, 942
MboII GAAGA 8 cut(s) 352, 419, 428, 550, 689, 1043, 1076, 1079
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 3 cut(s) 522, 610, 657
MluCI AATT 5 cut(s) 83, 152, 246, 368, 546
MlyI GAGTC 2 cut(s) 812, 1030
MroXI GAANNNNTTC 2 cut(s) 624, 932
MseI TTAA 4 cut(s) 501, 524, 842, 1074
MslI CAYNNNNRTG 1 cut(s) 648
MspI CCGG 2 cut(s) 564, 869
MspR9I CCNGG 1 cut(s) 230
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 1 cut(s) 865
NdeI CATATG 1 cut(s) 750
NdeII GATC 5 cut(s) 316, 382, 579, 640, 942
NlaIII CATG 6 cut(s) 537, 671, 799, 857, 901, 1021
NmuCI GTSAC 1 cut(s) 811
PaeR7I CTCGAG 1 cut(s) 278
PdmI GAANNNNTTC 2 cut(s) 624, 932
PfeI GAWTC 7 cut(s) 117, 132, 178, 215, 343, 541, 662
PflMI CCANNNNNTGG 1 cut(s) 386
PleI GAGTC 2 cut(s) 812, 1030
PpsI GAGTC 2 cut(s) 812, 1030
Ppu21I YACGTR 1 cut(s) 739
PshAI GACNNNNGTC 1 cut(s) 1040
Psp124BI GAGCTC 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspPI GGNCC 2 cut(s) 223, 884
PspXI VCTCGAGB 1 cut(s) 278
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 1 cut(s) 794
RsaNI GTAC 1 cut(s) 793
RseI CAYNNNNRTG 1 cut(s) 648
SacI GAGCTC 1 cut(s) 522
SaqAI TTAA 4 cut(s) 501, 524, 842, 1074
Sau3AI GATC 5 cut(s) 316, 382, 579, 640, 942
Sau96I GGNCC 2 cut(s) 223, 884
SchI GAGTC 2 cut(s) 812, 1030
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 3 cut(s) 522, 610, 657
SfaNI GCATC 5 cut(s) 80, 719, 754, 964, 1045
SfcI CTRYAG 2 cut(s) 112, 127
Sfr274I CTCGAG 1 cut(s) 278
SinI GGWCC 2 cut(s) 223, 884
SlaI CTCGAG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 648
SmlI CTYRAG 3 cut(s) 56, 278, 863
SmoI CTYRAG 3 cut(s) 56, 278, 863
Sse9I AATT 5 cut(s) 83, 152, 246, 368, 546
SsiI CCGC 2 cut(s) 445, 586
SspI AATATT 1 cut(s) 528
SspMI CTAG 2 cut(s) 768, 783
SstI GAGCTC 1 cut(s) 522
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 1 cut(s) 741
TaqI TCGA 6 cut(s) 88, 279, 315, 513, 639, 1050
TaqII GACCGA 1 cut(s) 872
TasI AATT 5 cut(s) 83, 152, 246, 368, 546
TfiI GAWTC 7 cut(s) 117, 132, 178, 215, 343, 541, 662
Tru1I TTAA 4 cut(s) 501, 524, 842, 1074
Tru9I TTAA 4 cut(s) 501, 524, 842, 1074
TscAI CASTG 1 cut(s) 635
TseFI GTSAC 1 cut(s) 811
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 6 cut(s) 322, 633, 675, 812, 886, 1077
TspRI CASTG 1 cut(s) 635
Van91I CCANNNNNTGG 1 cut(s) 386
VpaK11BI GGWCC 2 cut(s) 223, 884
XapI RAATTY 2 cut(s) 83, 546
XhoI CTCGAG 1 cut(s) 278
XmnI GAANNNNTTC 2 cut(s) 624, 932
XspI CTAG 2 cut(s) 768, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.