Rw7G008170

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016933
Physical Location & Seq
Forward (+)
7481982 .. 7483851
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G008170.1

Sequence Viewer

Length: 1860 bp
ATGAGAAGTCCATATCACAATCATCCATATAGTTTCAATCTTGGCTACTTCAATTCCTATGTTACAATGCGAATCCCAACAACAAAATCAAAATGGCTAACTCTGTTACAAAATTGCACACCAGAGAAGCCCAACCAGTTCAACCAAATCATATCCCACGCCATTGTTTCCGGCGTGTTAGCAAACCACCCATTCATATCAAGCCAAATCCTACTCCACTCACTCCCTCACGGCCTCGAATTTTCCCGCGCTGTTTTCTCTCAAATCCAAAACCCCAATGTCTTCGCCTGCAACTTCATCTTCAAAGCCTACTCCAACAGCCCAACCCCTCGAGAAACCCTCTCGCTGTACAACCTCGTGCGACGTCGTTTCACACACGTCTTGCCGGACAAGTATTCCTTTCCTTTTCTGTTCAAGGCCTGCGGTCGGGTTCAGCTCACTCGTAAAGGCCAAGAGCTTCACGCTTTGAGTTTCGCTCTCGGAGTTGATGGGGATGTTTTCGTTCAAAATGGGCTTATTTCTATGTACTCTGCGTGTGGGGAGCTTCAATCTGCGTTCAAAGTCTTTGAATTGATTCCGGTTTTCGTTCGGGACGTGGTGTCATGGAACAGCATTGTTAGTGGGTGTTTACAGAGTCATCGGAATTGGGACGCACTCCAGGTGTTTGTGAAAATGCTTGCGGATGGCTCGGCCAGGCCTGATGCGGTGGCTTTCGTCAATGCTCTGAATGCTTGTGCAAGGCTTGGTTCTGTTGATTTGGGGAGGAAGATTCATGGGTTGGTGTTGAGAAATGGATTTGATCTGGATGTGTTCTTGGGTTCGTCTTTGGTTGATATGTATGCAAAGTGTGGTGACATGGATGGTGCTCGAAAAGTTTTTGATAGAATGGGGAGAAGGAATGTGGTGTCTTGGACTTGTATGATTGCTGGTTATGTGCAGTCACATTGGTTCAAGGAAGCGATTGAGTTGTTCAGGGAGATGCAGTTGGATGGAGTTAAAGCAGACTCGGCATTGGTTGCTTGTGTTGTATCTGCGTGTGGGAATGCGGGTGCATTGGATCATGGAAGGTGGGTGCATACCTACTGCGAGAGAAGTGGCATTGAGATGAACCTCTCGGTGAAGAATGCTTTGATCGACATGTATTCAAAGTGCGGCGACTTTGAAAAGGCTCTCGAAATTTTTTGTGACATGACCATAAGAGATGTTGTTACATGGACTGCCATGATTATTGGTCTTGCTATGAATGGGGAATCTGATAGAGCACTAGAAATGTTTTCAGAAATGGAAGGGACAGGTTATGTTAGGCCAAATGAGGTCACATTCCTGGGGGTATTGTCTGCGTGCAGTCATGGAGGGTTTGTAAACAAGGGTTTGGGCTATTTGGAAGCCATGTCTGAAACATATAAGCTTACTCCTCGTGTTGATCACTATGGCTGTGTGATTGATCTTCTTGGTCGCGCTAATCTGTTGAATGAAGCTGCAAGGTTCATTAGTGCTATGCCTATTCAACCTGACGTGGTTATATGGCGGTCTCTGCTTTTTGCTTGTAGGACATATCAGAACTTAGAGCTGGCAGAGTTTGTTGCAAGAAAGATTGAGGAATTGGAACCAAGAAAGGCCGAGTCGAGTGTTTTGTTATCCCATCTATATGCTTCAGCATCAAGGTGGCTTGATGTGAACAAGACGAGGAAAGGTATGCCTCTTCATGGAATCCAGAAGAAGCCTGGTTGCTCTCTCATAGAAGTAGATGGTCTTGTTCATGAATTCACTGTTGCTGATTGTTCGCATTGTGAAAGAATCTCTATATACGAGACACTTGGAGCAATTAATAGAGTTATACAATCTGAAAGATTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04114 ko04914 ko05100 ko05131 map04110 map04114 map04914 map05100 map05131
Pfam Domains
Protein Families
HORMA PPR PPR_2

Protein Analysis

619

Amino Acids

69.3

Weight (kDa)

7.81

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 299 - 336 1.2e-07 PPR repeat family
PPR PF01535 302 - 332 1.6e-07 PPR repeat
PPR PF01535 375 - 398 9.8e-06 PPR repeat
PPR_2 PF13041 400 - 449 2.7e-08 PPR repeat family
PPR PF01535 403 - 430 1.6e-06 PPR repeat
E_motif PF20431 519 - 581 1.3e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016933)

Species Orthologous Gene IDs
malus_domestica MD00G1076200.v1.1 MD14G1144800.v1.1
prunus_persica Prupe.5G139200_v2.0.a1
pyrus_communis pycom06g11950 pycom14g12030
rosa_chinensis RchiOBHm_Chr7g0188361
rosa_laevigata RLG00000004699 RLG00000004705
rosa_multiflora Rmu_co8471191.1_g000001
rosa_roxburghii Rroxscaffold_3G00266450
rosa_rugosa Rorug06G0490100
rosa_samantha Rh7BG096300 Rh7CG096800
rosa_wichuraiana Rw7G008170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 367
AccII CGCG 2 cut(s) 249, 1458
AciI CCGC 7 cut(s) 247, 423, 680, 704, 1046, 1152, 1528
AclWI GGATC 1 cut(s) 1065
AcoI YGGCCR 1 cut(s) 690
AcsI RAATTY 3 cut(s) 239, 1176, 1763
AcuI CTGAAG 1 cut(s) 1638
AcyI GRCGYC 1 cut(s) 364
AfaI GTAC 2 cut(s) 350, 527
AfiI CCNNNNNNNGG 2 cut(s) 426, 1706
AflIII ACRYGT 2 cut(s) 376, 1137
AhdI GACNNNNNGTC 1 cut(s) 598
AjiI CACGTC 3 cut(s) 379, 595, 1516
AjnI CCWGG 4 cut(s) 657, 692, 1323, 1723
AluBI AGCT 6 cut(s) 436, 457, 544, 1408, 1478, 1570
AluI AGCT 6 cut(s) 436, 457, 544, 1408, 1478, 1570
Alw21I GWGCWC 2 cut(s) 868, 1264
Alw26I GTCTC 2 cut(s) 1536, 1805
AlwI GGATC 1 cut(s) 1065
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 7 cut(s) 232, 417, 448, 690, 695, 1304, 1617
ApeKI GCWGC 1 cut(s) 1478
ApoI RAATTY 3 cut(s) 239, 1176, 1763
AseI ATTAAT 1 cut(s) 1827
Asp700I GAANNNNTTC 1 cut(s) 573
AspLEI GCGC 2 cut(s) 251, 1460
AsuHPI GGTGA 2 cut(s) 863, 1129
AvaI CYCGRG 1 cut(s) 330
BauI CACGAG 2 cut(s) 356, 1416
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 868, 1264
BbvI GCAGC 1 cut(s) 1465
BccI CCATC 6 cut(s) 482, 677, 854, 983, 1650, 1742
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 4 cut(s) 659, 694, 1325, 1725
BclI TGATCA 1 cut(s) 1423
BcoDI GTCTC 2 cut(s) 1536, 1805
BfaI CTAG 1 cut(s) 1265
BisI GCNGC 2 cut(s) 1153, 1479
BlsI GCNGC 2 cut(s) 1154, 1480
Bme1390I CCNGG 4 cut(s) 659, 694, 1325, 1725
BmeRI GACNNNNNGTC 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 330
BmgBI CACGTC 3 cut(s) 379, 595, 1516
BmiI GGNNCC 1 cut(s) 1608
BmrFI CCNGG 4 cut(s) 659, 694, 1325, 1725
BmsI GCATC 3 cut(s) 691, 969, 1667
BpiI GAAGAC 1 cut(s) 274
BplI GAGNNNNNCTC 4 cut(s) 324, 356, 460, 492
BpmI CTGGAG 1 cut(s) 641
BsaHI GRCGYC 1 cut(s) 364
BsaI GGTCTC 1 cut(s) 1536
BsaJI CCNNGG 1 cut(s) 1324
BsaWI WCCGGW 1 cut(s) 577
BsaXI ACNNNNNCTCC 4 cut(s) 968, 998, 1344, 1374
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 1706
Bse1I ACTGG 1 cut(s) 136
BseBI CCWGG 4 cut(s) 659, 694, 1325, 1725
BseDI CCNNGG 1 cut(s) 1324
BseGI GGATG 6 cut(s) 22, 499, 688, 811, 865, 994
BseLI CCNNNNNNNGG 2 cut(s) 426, 1706
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 1 cut(s) 1404
BseXI GCAGC 1 cut(s) 1465
BsgI GTGCAG 2 cut(s) 956, 1363
Bsh1236I CGCG 2 cut(s) 249, 1458
Bsh1285I CGRYCG 1 cut(s) 427
BshFI GGCC 7 cut(s) 234, 419, 450, 692, 697, 1306, 1619
BsiEI CGRYCG 1 cut(s) 427
BsiHKAI GWGCWC 2 cut(s) 868, 1264
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 3 cut(s) 171, 386, 578
BslFI GGGAC 3 cut(s) 605, 662, 1303
BslI CCNNNNNNNGG 2 cut(s) 426, 1706
BsmAI GTCTC 2 cut(s) 1536, 1805
BsmFI GGGAC 3 cut(s) 605, 662, 1303
BsmI GAATGC 3 cut(s) 733, 1048, 1129
BsnI GGCC 7 cut(s) 234, 419, 450, 692, 697, 1306, 1619
Bso31I GGTCTC 1 cut(s) 1536
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 2 cut(s) 868, 1264
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 5 cut(s) 799, 1057, 1131, 1423, 1444
BspACI CCGC 7 cut(s) 247, 423, 680, 704, 1046, 1152, 1528
BspANI GGCC 7 cut(s) 234, 419, 450, 692, 697, 1306, 1619
BspFNI CGCG 2 cut(s) 249, 1458
BspHI TCATGA 1 cut(s) 1759
BspLI GGNNCC 1 cut(s) 1608
BspPI GGATC 1 cut(s) 1065
BspTNI GGTCTC 1 cut(s) 1536
BsrGI TGTACA 1 cut(s) 348
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 1324
BssMI GATC 5 cut(s) 799, 1057, 1131, 1423, 1444
BssNI GRCGYC 1 cut(s) 364
BssSI CACGAG 2 cut(s) 356, 1416
Bst2BI CACGAG 2 cut(s) 356, 1416
Bst2UI CCWGG 4 cut(s) 659, 694, 1325, 1725
Bst4CI ACNGT 1 cut(s) 1771
Bst6I CTCTTC 1 cut(s) 1707
BstACI GRCGYC 1 cut(s) 364
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstAUI TGTACA 1 cut(s) 348
BstC8I GCNNGC 5 cut(s) 289, 421, 678, 1342, 1572
BstDEI CTNAG 2 cut(s) 1564, 1857
BstF5I GGATG 6 cut(s) 22, 499, 688, 811, 865, 994
BstFNI CGCG 2 cut(s) 249, 1458
BstHHI GCGC 2 cut(s) 251, 1460
BstKTI GATC 5 cut(s) 802, 1060, 1134, 1426, 1447
BstMAI GTCTC 2 cut(s) 1536, 1805
BstMBI GATC 5 cut(s) 799, 1057, 1131, 1423, 1444
BstMCI CGRYCG 1 cut(s) 427
BstMWI GCNNNNNNNGC 4 cut(s) 728, 1007, 1016, 1534
BstNI CCWGG 4 cut(s) 659, 694, 1325, 1725
BstNSI RCATGY 1 cut(s) 1141
BstSCI CCNGG 4 cut(s) 657, 692, 1323, 1723
BstUI CGCG 2 cut(s) 249, 1458
BstV1I GCAGC 1 cut(s) 1465
BstV2I GAAGAC 1 cut(s) 274
BsuRI GGCC 7 cut(s) 234, 419, 450, 692, 697, 1306, 1619
BtrI CACGTC 3 cut(s) 379, 595, 1516
BtsCI GGATG 6 cut(s) 22, 499, 688, 811, 865, 994
BtsIMutI CAGTG 1 cut(s) 1767
Cac8I GCNNGC 5 cut(s) 289, 421, 678, 1342, 1572
CciI TCATGA 1 cut(s) 1759
CfoI GCGC 2 cut(s) 251, 1460
CseI GACGC 1 cut(s) 659
Csp6I GTAC 2 cut(s) 349, 526
CviQI GTAC 2 cut(s) 349, 526
DdeI CTNAG 2 cut(s) 1564, 1857
DpnI GATC 5 cut(s) 801, 1059, 1133, 1425, 1446
DpnII GATC 5 cut(s) 799, 1057, 1131, 1423, 1444
DriI GACNNNNNGTC 1 cut(s) 598
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 1 cut(s) 1707
Eam1105I GACNNNNNGTC 1 cut(s) 598
EarI CTCTTC 1 cut(s) 1707
Eco147I AGGCCT 2 cut(s) 419, 697
Eco31I GGTCTC 1 cut(s) 1536
Eco57I CTGAAG 1 cut(s) 1638
Eco88I CYCGRG 1 cut(s) 330
EcoRI GAATTC 1 cut(s) 1763
EcoRII CCWGG 4 cut(s) 657, 692, 1323, 1723
FalI AAGNNNNNCTT 3 cut(s) 383, 415, 1840
FaqI GGGAC 3 cut(s) 605, 662, 1303
FauI CCCGC 2 cut(s) 254, 1039
FbaI TGATCA 1 cut(s) 1423
Fnu4HI GCNGC 2 cut(s) 1153, 1479
FokI GGATG 6 cut(s) 9, 506, 695, 818, 872, 1001
Fsp4HI GCNGC 2 cut(s) 1153, 1479
FspBI CTAG 1 cut(s) 1265
GlaI GCGC 2 cut(s) 250, 1459
GluI GCNGC 2 cut(s) 1153, 1479
GsuI CTGGAG 1 cut(s) 641
HaeIII GGCC 7 cut(s) 234, 419, 450, 692, 697, 1306, 1619
HapII CCGG 3 cut(s) 171, 386, 578
HgaI GACGC 1 cut(s) 659
HhaI GCGC 2 cut(s) 251, 1460
Hin1I GRCGYC 1 cut(s) 364
Hin6I GCGC 2 cut(s) 249, 1458
HinP1I GCGC 2 cut(s) 249, 1458
HindIII AAGCTT 1 cut(s) 1406
HinfI GANTC 9 cut(s) 72, 574, 634, 769, 1004, 1250, 1622, 1710, 1797
HpaII CCGG 3 cut(s) 171, 386, 578
HphI GGTGA 2 cut(s) 863, 1129
Hpy166II GTNNAC 3 cut(s) 629, 1363, 1678
Hpy188I TCNGA 8 cut(s) 482, 642, 726, 1255, 1279, 1396, 1560, 1846
Hpy188III TCNNGA 6 cut(s) 332, 590, 803, 1172, 1714, 1760
Hpy8I GTNNAC 3 cut(s) 629, 1363, 1678
Hpy99I CGWCG 2 cut(s) 366, 369
HpyAV CCTTC 3 cut(s) 888, 1059, 1280
HpyCH4III ACNGT 1 cut(s) 1771
HpyCH4IV ACGT 4 cut(s) 364, 378, 594, 1515
HpyF10VI GCNNNNNNNGC 4 cut(s) 728, 1007, 1016, 1534
HpyF3I CTNAG 2 cut(s) 1564, 1857
HpySE526I ACGT 4 cut(s) 364, 378, 594, 1515
Hsp92I GRCGYC 1 cut(s) 364
HspAI GCGC 2 cut(s) 249, 1458
Ksp22I TGATCA 1 cut(s) 1423
Kzo9I GATC 5 cut(s) 799, 1057, 1131, 1423, 1444
LmnI GCTCC 2 cut(s) 541, 1820
Lsp1109I GCAGC 1 cut(s) 1465
LweI GCATC 3 cut(s) 691, 969, 1667
MaeI CTAG 1 cut(s) 1265
MaeII ACGT 4 cut(s) 364, 378, 594, 1515
MaeIII GTNAC 7 cut(s) 61, 105, 851, 939, 1184, 1207, 1315
MalI GATC 5 cut(s) 801, 1059, 1133, 1425, 1446
MboI GATC 5 cut(s) 799, 1057, 1131, 1423, 1444
MboII GAAGA 7 cut(s) 274, 292, 778, 1132, 1439, 1694, 1729
MhlI GDGCHC 2 cut(s) 868, 1264
MluCI AATT 9 cut(s) 52, 112, 239, 569, 643, 1176, 1601, 1763, 1824
MlyI GAGTC 3 cut(s) 643, 998, 1631
MmeI TCCRAC 2 cut(s) 339, 966
MroXI GAANNNNTTC 1 cut(s) 573
MseI TTAA 2 cut(s) 996, 1827
MslI CAYNNNNRTG 3 cut(s) 1103, 1647, 1663
MspI CCGG 3 cut(s) 171, 386, 578
MspR9I CCNGG 4 cut(s) 659, 694, 1325, 1725
Mva1269I GAATGC 3 cut(s) 733, 1048, 1129
MvaI CCWGG 4 cut(s) 659, 694, 1325, 1725
MvnI CGCG 2 cut(s) 249, 1458
MwoI GCNNNNNNNGC 4 cut(s) 728, 1007, 1016, 1534
NdeII GATC 5 cut(s) 799, 1057, 1131, 1423, 1444
NlaIV GGNNCC 1 cut(s) 1608
NmeAIII GCCGAG 3 cut(s) 668, 986, 1645
NmuCI GTSAC 4 cut(s) 851, 939, 1184, 1315
NspI RCATGY 1 cut(s) 1141
PaeR7I CTCGAG 1 cut(s) 330
PagI TCATGA 1 cut(s) 1759
PceI AGGCCT 2 cut(s) 419, 697
PciI ACATGT 1 cut(s) 1137
PcsI WCGNNNNNNNCGW 1 cut(s) 591
PctI GAATGC 3 cut(s) 733, 1048, 1129
PdmI GAANNNNTTC 1 cut(s) 573
PfeI GAWTC 6 cut(s) 72, 574, 769, 1250, 1710, 1797
PkrI GCNGC 2 cut(s) 1154, 1480
PleI GAGTC 3 cut(s) 642, 998, 1630
PpsI GAGTC 3 cut(s) 642, 998, 1630
PscI ACATGT 1 cut(s) 1137
PshBI ATTAAT 1 cut(s) 1827
Psp6I CCWGG 4 cut(s) 657, 692, 1323, 1723
PspGI CCWGG 4 cut(s) 657, 692, 1323, 1723
PspN4I GGNNCC 1 cut(s) 1608
RsaI GTAC 2 cut(s) 350, 527
RsaNI GTAC 2 cut(s) 349, 526
RseI CAYNNNNRTG 3 cut(s) 1103, 1647, 1663
SaqAI TTAA 2 cut(s) 996, 1827
SatI GCNGC 2 cut(s) 1153, 1479
Sau3AI GATC 5 cut(s) 799, 1057, 1131, 1423, 1444
SchI GAGTC 3 cut(s) 643, 998, 1631
ScrFI CCNGG 4 cut(s) 659, 694, 1325, 1725
SduI GDGCHC 2 cut(s) 868, 1264
SfaNI GCATC 3 cut(s) 691, 969, 1667
Sfr274I CTCGAG 1 cut(s) 330
SlaI CTCGAG 1 cut(s) 330
SmiMI CAYNNNNRTG 3 cut(s) 1103, 1647, 1663
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
Sse9I AATT 9 cut(s) 52, 112, 239, 569, 643, 1176, 1601, 1763, 1824
SseBI AGGCCT 2 cut(s) 419, 697
SsiI CCGC 7 cut(s) 247, 423, 680, 704, 1046, 1152, 1528
SspMI CTAG 1 cut(s) 1265
StuI AGGCCT 2 cut(s) 419, 697
StyD4I CCNGG 4 cut(s) 657, 692, 1323, 1723
TaaI ACNGT 1 cut(s) 1771
TaiI ACGT 4 cut(s) 367, 381, 597, 1518
TaqI TCGA 6 cut(s) 237, 331, 868, 1134, 1173, 1625
TasI AATT 9 cut(s) 52, 112, 239, 569, 643, 1176, 1601, 1763, 1824
TatI WGTACW 2 cut(s) 348, 525
TauI GCSGC 1 cut(s) 1155
TfiI GAWTC 6 cut(s) 72, 574, 769, 1250, 1710, 1797
Tru1I TTAA 2 cut(s) 996, 1827
Tru9I TTAA 2 cut(s) 996, 1827
TscAI CASTG 1 cut(s) 1774
TseFI GTSAC 4 cut(s) 851, 939, 1184, 1315
TseI GCWGC 1 cut(s) 1478
Tsp45I GTSAC 4 cut(s) 851, 939, 1184, 1315
TspRI CASTG 1 cut(s) 1774
VspI ATTAAT 1 cut(s) 1827
XapI RAATTY 3 cut(s) 239, 1176, 1763
XceI RCATGY 1 cut(s) 1141
XcmI CCANNNNNNNNNTGG 1 cut(s) 1721
XhoI CTCGAG 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 573
XspI CTAG 1 cut(s) 1265
ZraI GACGTC 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.