AT1G05280

Fringe-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013240
Physical Location & Seq
Reverse (-)
1534830 .. 1537602
2773 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05280.1

Sequence Viewer

Length: 1476 bp
ATGTGTCTTCCATTTCGACCCGTCTCGAAAACATATTATTCGTCTTCTTCTTCGACTATCGTGACGCGTATGGTAAACTGCATACTACTTTTTGTATTCATTTTGTTGGTCTACCTCCTCATCTCGGCCTCAAGACTTCAATCTAAAAACTCTATTCATGCATACTTCTCATCTTCTGATCAAGATCAATCTCAGAGCCCGACAAAAATCGAGCACATCGTCTTTGGGATAGGCTCAAGCGCGATCTCGTGGCGTGCACGTAGAGAATACGTTAAGCTTTGGTGGGATGCTCAGAAAATGAGAGGCTGCGTCTTTGTTGAGCGTCCCTTGCCGTCTTCTCAAAACCACACAGATTCATATCTCCTTCCTCCGGTTTGTGTTTCCCAAGATACTTCCCGGTTCAGGTACACTTGGAGAGGCGGTGACAGAAACGCGATCCGAATCGCGCGGTGCGTTCTAGAAACTGTCAGGCTATTCAACACCTCCTCAGAAGAAGTAAGATGGTACGTATTTGGAGACGACGACACGATTTTCATTCCTGAAAACCTCGCTCGAACTCTCTCCAAATACGACCACACATCATGGTATTACATAGGATCAACCTCGGAGATTTATCACCAGAACTCTATGTTCGGACACGACATGGCGTTCGGGGGTGGTGGGTACGCTTTAAGCAGCTCATTGGCTAATGTTTTAGCTAGAAATTTCGATTCTTGTATCGAACGATATCCACATTTGTACGGAGGAGACTCTAGGGTTTATGCTTGTGTGCTTGAGCTTGGGGTTGGATTGTCTAAAGAGCCTGGCTTCCATCAGTTTGATGTGAGAGGAAATGCATTGGGAATATTGACATCACATTCAACGAGACCGTTGGTGTCACTACACCACATGGCTCACATTGACCCAATCTTTCCAAACTCAACCACTTTCTCCGCGGTCCGCCACCTTTTCTCCGCCGTACAACTCGACCCTCTCCGTATTTTTCAACTATCCGTTTGCTACGACCGTTGGTACTCTTGGACCATCTCCGTCTCTTGGGGATATACTGTTCAGATTGATGGCAGGCATTTGTTTCTACGGGATGTTTTGCGGGCACAAGAGACGTTCCGACCATGGCAAAAATCAGGCGGATTAGCAAGTGTTTACACATTCAACACAAGAGAGATTCATCGTGATCCTTGCCAACGACCTGTCACTTTCTACATGCAACATGTTTCTTCTAGTAGCCATGATGGAACCATTAAAAGTGTTTATAAGCAAGCTTACGAGAATTGCACCTACGATCCTGTTACATCGCCTCGCAAAATTCATGAGATTAGAGTGTTTTCAAGAAGACTCGATCCCAATATCAGACAATTGAAGGCTCCCAGAAGGCAGTGTTGTGATATATTACCAACTTCTTCAACTGGTGGCAACATAATGGAAATTGGACTGCGAGAGTGCAAAGAGGATGAGCTCATCTATATTCATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

491

Amino Acids

56.36

Weight (kDa)

8.86

Isoelectric Point (pI)

58.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 143 - 272 3.1e-10 Fringe-like
DUF604 PF04646 207 - 461 6.7e-98 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05280 AT1G05280
fragaria_vesca FvH4_6g43600
malus_domestica MD09G1104300.v1.1 MD17G1092000.v1.1
prunus_persica Prupe.3G221300_v2.0.a1
pyrus_communis pycom09g02750 pycom17g08910
rosa_chinensis RchiOBHm_Chr2g0160191
rosa_laevigata RLG00000021203
rosa_multiflora Rmu_sc0000403.1_g000007
rosa_roxburghii Rroxscaffold_2G00089970
rosa_rugosa Rorug02G0482300
rosa_samantha Rh2AG547700 Rh2BG560900 Rh2CG531500 Rh2DG569600
rosa_wichuraiana Rw2G045290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1254
AccI GTMKAC 1 cut(s) 111
AccII CGCG 6 cut(s) 67, 242, 434, 446, 448, 935
AciI CCGC 8 cut(s) 420, 448, 933, 935, 940, 954, 1090, 1128
AclWI GGATC 5 cut(s) 430, 604, 1169, 1277, 1334
AcsI RAATTY 2 cut(s) 703, 1305
AfaI GTAC 6 cut(s) 407, 506, 665, 740, 960, 1013
AfiI CCNNNNNNNGG 4 cut(s) 124, 370, 402, 1035
AflIII ACRYGT 2 cut(s) 65, 1210
AgsI TTSAA 8 cut(s) 140, 478, 861, 986, 1153, 1329, 1360, 1404
AjnI CCWGG 1 cut(s) 802
AloI GAACNNNNNNTCC 2 cut(s) 1032, 1064
AluBI AGCT 6 cut(s) 277, 678, 698, 778, 1262, 1456
AluI AGCT 6 cut(s) 277, 678, 698, 778, 1262, 1456
Alw21I GWGCWC 3 cut(s) 216, 259, 1458
Alw26I GTCTC 6 cut(s) 28, 510, 741, 859, 1036, 1094
Alw44I GTGCAC 1 cut(s) 255
AlwI GGATC 5 cut(s) 430, 604, 1169, 1277, 1334
AoxI GGCC 1 cut(s) 126
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 2 cut(s) 306, 675
ApoI RAATTY 2 cut(s) 703, 1305
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AspLEI GCGC 2 cut(s) 242, 448
AspS9I GGNCC 2 cut(s) 937, 1020
AsuC2I CCSGG 1 cut(s) 397
AsuHPI GGTGA 2 cut(s) 434, 608
AvaII GGWCC 2 cut(s) 937, 1020
BaeGI GKGCMC 2 cut(s) 259, 1096
BanII GRGCYC 2 cut(s) 200, 1458
BauI CACGAG 1 cut(s) 247
BbsI GAAGAC 3 cut(s) 36, 327, 1339
Bbv12I GWGCWC 3 cut(s) 216, 259, 1458
BbvI GCAGC 2 cut(s) 293, 687
BccI CCATC 5 cut(s) 495, 819, 1031, 1052, 1226
BceAI ACGGC 2 cut(s) 316, 941
BciT130I CCWGG 1 cut(s) 804
BclI TGATCA 1 cut(s) 178
BcnI CCSGG 1 cut(s) 397
BcoDI GTCTC 6 cut(s) 28, 510, 741, 859, 1036, 1094
BfaI CTAG 4 cut(s) 458, 699, 753, 1221
BisI GCNGC 2 cut(s) 307, 676
BlsI GCNGC 2 cut(s) 308, 677
Bme1390I CCNGG 2 cut(s) 397, 804
Bme18I GGWCC 2 cut(s) 937, 1020
BmgT120I GGNCC 2 cut(s) 937, 1020
BmiI GGNNCC 2 cut(s) 1237, 1365
BmrFI CCNGG 2 cut(s) 397, 804
BmsI GCATC 1 cut(s) 277
BpiI GAAGAC 3 cut(s) 36, 327, 1339
BpuEI CTTGAG 3 cut(s) 115, 220, 794
BpuMI CCSGG 1 cut(s) 397
BsaAI YACGTR 2 cut(s) 260, 508
BsaBI GATNNNNATC 3 cut(s) 183, 357, 440
BsaI GGTCTC 1 cut(s) 859
BsaJI CCNNGG 3 cut(s) 603, 933, 1112
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 4 cut(s) 406, 436, 935, 965
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 370, 402, 1035
Bse1I ACTGG 1 cut(s) 1411
Bse8I GATNNNNATC 3 cut(s) 183, 357, 440
BseBI CCWGG 1 cut(s) 804
BseDI CCNNGG 3 cut(s) 603, 933, 1112
BseGI GGATG 4 cut(s) 292, 1087, 1456, 1468
BseJI GATNNNNATC 3 cut(s) 183, 357, 440
BseLI CCNNNNNNNGG 4 cut(s) 124, 370, 402, 1035
BseMII CTCAG 3 cut(s) 206, 305, 501
BseNI ACTGG 1 cut(s) 1411
BseRI GAGGAG 3 cut(s) 107, 475, 759
BseSI GKGCMC 2 cut(s) 259, 1096
BseXI GCAGC 2 cut(s) 293, 687
Bsh1236I CGCG 6 cut(s) 67, 242, 434, 446, 448, 935
Bsh1285I CGRYCG 1 cut(s) 1006
BshFI GGCC 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 1006
BsiHKAI GWGCWC 3 cut(s) 216, 259, 1458
BsiSI CCGG 2 cut(s) 371, 397
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 4 cut(s) 124, 370, 402, 1035
BsmAI GTCTC 6 cut(s) 28, 510, 741, 859, 1036, 1094
BsmBI CGTCTC 4 cut(s) 28, 510, 1036, 1094
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 859
Bsp1286I GDGCHC 5 cut(s) 200, 216, 259, 1096, 1458
Bsp143I GATC 8 cut(s) 178, 184, 243, 435, 596, 1174, 1282, 1339
Bsp19I CCATGG 1 cut(s) 1112
BspACI CCGC 8 cut(s) 420, 448, 933, 935, 940, 954, 1090, 1128
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 3 cut(s) 205, 304, 500
BspFNI CGCG 6 cut(s) 67, 242, 434, 446, 448, 935
BspHI TCATGA 1 cut(s) 1309
BspLI GGNNCC 2 cut(s) 1237, 1365
BspPI GGATC 5 cut(s) 430, 604, 1169, 1277, 1334
BspTNI GGTCTC 1 cut(s) 859
BsrI ACTGG 1 cut(s) 1411
BssECI CCNNGG 3 cut(s) 603, 933, 1112
BssMI GATC 8 cut(s) 178, 184, 243, 435, 596, 1174, 1282, 1339
BssSI CACGAG 1 cut(s) 247
BssT1I CCWWGG 1 cut(s) 1112
Bst2BI CACGAG 1 cut(s) 247
Bst2UI CCWGG 1 cut(s) 804
Bst4CI ACNGT 4 cut(s) 466, 870, 1007, 1048
BstBAI YACGTR 2 cut(s) 260, 508
BstC8I GCNNGC 4 cut(s) 255, 1064, 1092, 1260
BstDEI CTNAG 3 cut(s) 192, 291, 487
BstDSI CCRYGG 2 cut(s) 933, 1112
BstF5I GGATG 4 cut(s) 292, 1087, 1456, 1468
BstFNI CGCG 6 cut(s) 67, 242, 434, 446, 448, 935
BstHHI GCGC 2 cut(s) 242, 448
BstKTI GATC 8 cut(s) 181, 187, 246, 438, 599, 1177, 1285, 1342
BstMAI GTCTC 6 cut(s) 28, 510, 741, 859, 1036, 1094
BstMBI GATC 8 cut(s) 178, 184, 243, 435, 596, 1174, 1282, 1339
BstMCI CGRYCG 1 cut(s) 1006
BstMWI GCNNNNNNNGC 1 cut(s) 328
BstNI CCWGG 1 cut(s) 804
BstNSI RCATGY 2 cut(s) 1207, 1214
BstSCI CCNGG 2 cut(s) 395, 802
BstSLI GKGCMC 2 cut(s) 259, 1096
BstSNI TACGTA 1 cut(s) 508
BstUI CGCG 6 cut(s) 67, 242, 434, 446, 448, 935
BstV1I GCAGC 2 cut(s) 293, 687
BstV2I GAAGAC 3 cut(s) 36, 327, 1339
BsuRI GGCC 1 cut(s) 128
BtgI CCRYGG 2 cut(s) 933, 1112
BtgZI GCGATG 1 cut(s) 1278
BtsCI GGATG 4 cut(s) 292, 1087, 1456, 1468
BtsI GCAGTG 1 cut(s) 1382
BtsIMutI CAGTG 1 cut(s) 1382
Cac8I GCNNGC 4 cut(s) 255, 1064, 1092, 1260
CciI TCATGA 1 cut(s) 1309
CfoI GCGC 2 cut(s) 242, 448
Cfr13I GGNCC 2 cut(s) 937, 1020
Cfr42I CCGCGG 1 cut(s) 936
CpoI CGGWCCG 1 cut(s) 937
CseI GACGC 3 cut(s) 73, 298, 311
Csp6I GTAC 6 cut(s) 406, 505, 664, 739, 959, 1012
CspI CGGWCCG 1 cut(s) 937
CviAII CATG 9 cut(s) 158, 582, 643, 889, 1113, 1204, 1211, 1229, 1310
CviQI GTAC 6 cut(s) 406, 505, 664, 739, 959, 1012
DdeI CTNAG 3 cut(s) 192, 291, 487
DpnI GATC 8 cut(s) 180, 186, 245, 437, 598, 1176, 1284, 1341
DpnII GATC 8 cut(s) 178, 184, 243, 435, 596, 1174, 1282, 1339
EciI GGCGGA 3 cut(s) 929, 943, 1143
Ecl136II GAGCTC 1 cut(s) 1456
Eco105I TACGTA 1 cut(s) 508
Eco130I CCWWGG 1 cut(s) 1112
Eco24I GRGCYC 2 cut(s) 200, 1458
Eco31I GGTCTC 1 cut(s) 859
Eco32I GATATC 1 cut(s) 728
Eco47I GGWCC 2 cut(s) 937, 1020
Eco53kI GAGCTC 1 cut(s) 1456
EcoICRI GAGCTC 1 cut(s) 1456
EcoRII CCWGG 1 cut(s) 802
EcoRV GATATC 1 cut(s) 728
EcoT14I CCWWGG 1 cut(s) 1112
EcoT22I ATGCAT 2 cut(s) 163, 838
EcoT38I GRGCYC 2 cut(s) 200, 1458
ErhI CCWWGG 1 cut(s) 1112
Esp3I CGTCTC 4 cut(s) 28, 510, 1036, 1094
FaeI CATG 9 cut(s) 161, 585, 646, 892, 1116, 1207, 1214, 1232, 1313
FaqI GGGAC 1 cut(s) 309
FatI CATG 9 cut(s) 157, 581, 642, 888, 1112, 1203, 1210, 1228, 1309
FauI CCCGC 1 cut(s) 1083
FbaI TGATCA 1 cut(s) 178
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 2 cut(s) 307, 676
FokI GGATG 4 cut(s) 299, 1094, 1455, 1463
FriOI GRGCYC 2 cut(s) 200, 1458
Fsp4HI GCNGC 2 cut(s) 307, 676
FspBI CTAG 4 cut(s) 458, 699, 753, 1221
GlaI GCGC 2 cut(s) 241, 447
GluI GCNGC 2 cut(s) 307, 676
HaeIII GGCC 1 cut(s) 128
HapII CCGG 2 cut(s) 371, 397
HgaI GACGC 3 cut(s) 73, 298, 311
HhaI GCGC 2 cut(s) 242, 448
Hin1II CATG 9 cut(s) 161, 585, 646, 892, 1116, 1207, 1214, 1232, 1313
Hin6I GCGC 2 cut(s) 240, 446
HinP1I GCGC 2 cut(s) 240, 446
HindIII AAGCTT 2 cut(s) 275, 1260
HinfI GANTC 6 cut(s) 353, 441, 710, 749, 1165, 1335
HpaII CCGG 2 cut(s) 371, 397
HphI GGTGA 2 cut(s) 434, 608
Hpy166II GTNNAC 5 cut(s) 76, 112, 257, 408, 1144
Hpy188III TCNNGA 9 cut(s) 25, 61, 132, 182, 458, 539, 1172, 1310, 1329
Hpy8I GTNNAC 5 cut(s) 76, 112, 257, 408, 1144
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 3 cut(s) 374, 1354, 1365
HpyCH4III ACNGT 4 cut(s) 466, 870, 1007, 1048
HpyCH4IV ACGT 4 cut(s) 259, 270, 507, 1103
HpyCH4V TGCA 7 cut(s) 81, 161, 257, 836, 1207, 1275, 1443
HpyF10VI GCNNNNNNNGC 1 cut(s) 328
HpyF3I CTNAG 3 cut(s) 192, 291, 487
HpySE526I ACGT 4 cut(s) 259, 270, 507, 1103
Hsp92II CATG 9 cut(s) 161, 585, 646, 892, 1116, 1207, 1214, 1232, 1313
HspAI GCGC 2 cut(s) 240, 446
Ksp22I TGATCA 1 cut(s) 178
KspI CCGCGG 1 cut(s) 936
Kzo9I GATC 8 cut(s) 178, 184, 243, 435, 596, 1174, 1282, 1339
LmnI GCTCC 1 cut(s) 1369
Lsp1109I GCAGC 2 cut(s) 293, 687
LweI GCATC 1 cut(s) 277
MaeI CTAG 4 cut(s) 458, 699, 753, 1221
MaeII ACGT 4 cut(s) 259, 270, 507, 1103
MaeIII GTNAC 5 cut(s) 61, 422, 876, 1192, 1288
MalI GATC 8 cut(s) 180, 186, 245, 437, 598, 1176, 1284, 1341
MboI GATC 8 cut(s) 178, 184, 243, 435, 596, 1174, 1282, 1339
MboII GAAGA 9 cut(s) 36, 39, 42, 165, 327, 503, 1209, 1344, 1392
MfeI CAATTG 1 cut(s) 1355
MhlI GDGCHC 5 cut(s) 200, 216, 259, 1096, 1458
MluCI AATT 5 cut(s) 703, 1270, 1305, 1355, 1425
MluI ACGCGT 1 cut(s) 65
MlyI GAGTC 2 cut(s) 743, 1329
MmeI TCCRAC 2 cut(s) 766, 1132
Mph1103I ATGCAT 2 cut(s) 163, 838
MseI TTAA 3 cut(s) 273, 671, 1242
MspA1I CMGCKG 1 cut(s) 935
MspI CCGG 2 cut(s) 371, 397
MspR9I CCNGG 2 cut(s) 397, 804
MunI CAATTG 1 cut(s) 1355
MvaI CCWGG 1 cut(s) 804
MvnI CGCG 6 cut(s) 67, 242, 434, 446, 448, 935
MwoI GCNNNNNNNGC 1 cut(s) 328
NciI CCSGG 1 cut(s) 397
NcoI CCATGG 1 cut(s) 1112
NdeII GATC 8 cut(s) 178, 184, 243, 435, 596, 1174, 1282, 1339
NlaIII CATG 9 cut(s) 161, 585, 646, 892, 1116, 1207, 1214, 1232, 1313
NlaIV GGNNCC 2 cut(s) 1237, 1365
NmeAIII GCCGAG 1 cut(s) 104
NmuCI GTSAC 4 cut(s) 61, 422, 876, 1192
NsiI ATGCAT 2 cut(s) 163, 838
NspI RCATGY 2 cut(s) 1207, 1214
PagI TCATGA 1 cut(s) 1309
PciI ACATGT 1 cut(s) 1210
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 450
PfeI GAWTC 4 cut(s) 353, 441, 710, 1165
PkrI GCNGC 2 cut(s) 308, 677
PleI GAGTC 2 cut(s) 743, 1329
PpsI GAGTC 2 cut(s) 743, 1329
Ppu21I YACGTR 2 cut(s) 260, 508
PscI ACATGT 1 cut(s) 1210
PsiI TTATAA 1 cut(s) 1254
Psp124BI GAGCTC 1 cut(s) 1458
Psp6I CCWGG 1 cut(s) 802
PspGI CCWGG 1 cut(s) 802
PspN4I GGNNCC 2 cut(s) 1237, 1365
PspPI GGNCC 2 cut(s) 937, 1020
RsaI GTAC 6 cut(s) 407, 506, 665, 740, 960, 1013
RsaNI GTAC 6 cut(s) 406, 505, 664, 739, 959, 1012
Rsr2I CGGWCCG 1 cut(s) 937
RsrII CGGWCCG 1 cut(s) 937
SacI GAGCTC 1 cut(s) 1458
SacII CCGCGG 1 cut(s) 936
SaqAI TTAA 3 cut(s) 273, 671, 1242
SatI GCNGC 2 cut(s) 307, 676
Sau3AI GATC 8 cut(s) 178, 184, 243, 435, 596, 1174, 1282, 1339
Sau96I GGNCC 2 cut(s) 937, 1020
SchI GAGTC 2 cut(s) 743, 1329
ScrFI CCNGG 2 cut(s) 397, 804
SduI GDGCHC 5 cut(s) 200, 216, 259, 1096, 1458
SfaNI GCATC 1 cut(s) 277
Sfr303I CCGCGG 1 cut(s) 936
SgrBI CCGCGG 1 cut(s) 936
SinI GGWCC 2 cut(s) 937, 1020
SmlI CTYRAG 3 cut(s) 130, 235, 773
SmoI CTYRAG 3 cut(s) 130, 235, 773
SnaBI TACGTA 1 cut(s) 508
Sse9I AATT 5 cut(s) 703, 1270, 1305, 1355, 1425
SsiI CCGC 8 cut(s) 420, 448, 933, 935, 940, 954, 1090, 1128
SspI AATATT 1 cut(s) 846
SspMI CTAG 4 cut(s) 458, 699, 753, 1221
SstI GAGCTC 1 cut(s) 1458
StyD4I CCNGG 2 cut(s) 395, 802
StyI CCWWGG 1 cut(s) 1112
TaaI ACNGT 4 cut(s) 466, 870, 1007, 1048
TaiI ACGT 4 cut(s) 262, 273, 510, 1106
TaqI TCGA 9 cut(s) 16, 26, 53, 210, 553, 708, 720, 966, 1338
TasI AATT 5 cut(s) 703, 1270, 1305, 1355, 1425
TfiI GAWTC 4 cut(s) 353, 441, 710, 1165
Tru1I TTAA 3 cut(s) 273, 671, 1242
Tru9I TTAA 3 cut(s) 273, 671, 1242
TscAI CASTG 1 cut(s) 1382
TseFI GTSAC 4 cut(s) 61, 422, 876, 1192
TseI GCWGC 2 cut(s) 306, 675
Tsp45I GTSAC 4 cut(s) 61, 422, 876, 1192
TspDTI ATGAA 7 cut(s) 88, 146, 345, 523, 1157, 1298, 1457
TspGWI ACGGA 4 cut(s) 756, 965, 982, 1018
TspRI CASTG 1 cut(s) 1382
VneI GTGCAC 1 cut(s) 255
VpaK11BI GGWCC 2 cut(s) 937, 1020
XapI RAATTY 2 cut(s) 703, 1305
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 2 cut(s) 1207, 1214
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 4 cut(s) 458, 699, 753, 1221
Zsp2I ATGCAT 2 cut(s) 163, 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.