Rh2BG560900

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013240
Physical Location & Seq
Forward (+)
77203952 .. 77205557
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG560900.1

Sequence Viewer

Length: 1428 bp
ATGCAGCTTATTCCATCACCCACCAAAACCCAAGTTTCTTCTCGCTTGATAAACCTCTACATAATCTCATCTTCTGTCTGCACGCTCTTTCTTTTCGTATCATTCTTCCTGGTGCTTACAAGCTCCAAGACTTCGTATATTTCGTCCCAAGATGGATATGCGACAACTCCTACTTCTCTTGACCATGTTGTGTTCGGAATTGCTTCGAACCAGAAATCATGGACGAGTCAGAGGAAGGAGTATGTTAGGCTCTGGTGGAAGCACCATTCCATGAGGGGGTGTGTTTTTCTCGAAAGCCTTCCACCTGATCATCAACACAATCATACCAATGATACTACTCTTCCCCCGGTATGCATCTCCGGGGACACTTCCAGATTTCGTTACACTTACAAAGGTGGTCTCCGGTCTGCAATTCGCGTGGCACGAGTTGTTTCCGAGACTGTGGCACTCAATCACTCCAATGTGCGTTGGTATGTCTTCGGAGATGATGACACGGTTTTCATCCCGGAGAATTTGGTGAAGACTCTTTCCAAGTATGATCATGAGCTCTGGTACTATATTGGCGCCAACTCAGAGATCTATGAGCAGAACAGAATTTTCGGATTTGGAATGGCTTTTGGCGGTGCAGGTTTTGCTATGAGTTACCCTTTGGCAAAAGCATTGGCAAAGGTGTTTGATTCATGTATAGAAAGATACCCACATCTCTATGGCAGTGACTCAAGGATCTCATCTTGCTTGGCTGAGCTTGGCGTAGGCTTAACTCACGAGCCAGGTTTTCATCAGAATGATCTTCGTGGTGATACGTTTGGCCTTTTGGCTTCGCATCCATTAGCACCCTTGGTATCCCTGCATCATCTTGAGCACACAGACCCTATCTTCCCCAACAGGACAACCATGCAAGCTCTGCAGCATTTATTCAAAGCTGTGAATGTTGATCCTCATAGGATTTTACAGAAGACCGTTTGCTATGATAGATGGTTCTCATGGACAGTTTCGGTGTCATGGGGTTATGCTGTTCAGATATACGGAAACCATATGCCTCTGCGTGACGTCCTCCCGGTGCAAGAGACATTCAAGCCGTGGAAAAAGGGACCTGTTTTGTCTGGAGTTTATACTTTTAATACAAGAGAACATCACCCGGATCCCTGTCGAAGACCAGTTGTTTTCTTTCTTGATCATGTGTCATCGAGAGAGGATGGAATCATGAGCATCTATAAGAAATCTTATGAAAATTGCTCCTATGACATGGCTTCCCCCAGAAAGCTTGAGGAGGTCAGAGTGCTATCACAAAAGCTGGACCTTGACATCAAACAGTTGCAAGCTCCAAGAAGGCATTGTTGTGACATATTACCTTCTACCGGTGGTGGGGTGATGGACATTGTAATCAGAGAATGCAAACATGAAGAATTAACATACATGCATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

475

Amino Acids

54.21

Weight (kDa)

8.21

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 135 - 262 7.1e-13 Fringe-like
DUF604 PF04646 196 - 447 1.4e-107 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05280 AT1G05280
fragaria_vesca FvH4_6g43600
malus_domestica MD09G1104300.v1.1 MD17G1092000.v1.1
prunus_persica Prupe.3G221300_v2.0.a1
pyrus_communis pycom09g02750 pycom17g08910
rosa_chinensis RchiOBHm_Chr2g0160191
rosa_laevigata RLG00000021203
rosa_multiflora Rmu_sc0000403.1_g000007
rosa_roxburghii Rroxscaffold_2G00089970
rosa_rugosa Rorug02G0482300
rosa_samantha Rh2AG547700 Rh2BG560900 Rh2CG531500 Rh2DG569600
rosa_wichuraiana Rw2G045290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1053
Acc36I ACCTGC 1 cut(s) 617
AccB1I GGYRCC 1 cut(s) 563
AccII CGCG 1 cut(s) 417
AciI CCGC 1 cut(s) 621
AclWI GGATC 4 cut(s) 731, 929, 1136, 1149
AcsI RAATTY 2 cut(s) 511, 594
AcyI GRCGYC 2 cut(s) 564, 1050
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 3 cut(s) 276, 1358, 1365
AgeI ACCGGT 1 cut(s) 1358
AgsI TTSAA 2 cut(s) 919, 1075
AjnI CCWGG 2 cut(s) 108, 769
AluBI AGCT 9 cut(s) 7, 123, 547, 745, 902, 923, 1264, 1294, 1322
AluI AGCT 9 cut(s) 7, 123, 547, 745, 902, 923, 1264, 1294, 1322
Alw21I GWGCWC 2 cut(s) 549, 864
Alw26I GTCTC 3 cut(s) 404, 431, 1061
AoxI GGCC 1 cut(s) 808
ApeKI GCWGC 2 cut(s) 4, 907
ApoI RAATTY 2 cut(s) 511, 594
AsiGI ACCGGT 1 cut(s) 1358
Asp700I GAANNNNTTC 2 cut(s) 202, 297
AspLEI GCGC 1 cut(s) 566
AspS9I GGNCC 2 cut(s) 1091, 1297
AsuC2I CCSGG 5 cut(s) 347, 361, 506, 1058, 1139
AsuHPI GGTGA 5 cut(s) 9, 529, 809, 1127, 1381
AsuII TTCGAA 1 cut(s) 206
AvaII GGWCC 2 cut(s) 1091, 1297
BamHI GGATCC 1 cut(s) 1141
BanI GGYRCC 1 cut(s) 563
BanII GRGCYC 1 cut(s) 549
BauI CACGAG 2 cut(s) 423, 764
BbsI GAAGAC 4 cut(s) 469, 527, 962, 1159
Bbv12I GWGCWC 2 cut(s) 549, 864
BbvI GCAGC 2 cut(s) 16, 919
BccI CCATC 5 cut(s) 22, 146, 969, 1190, 1366
BceAI ACGGC 1 cut(s) 1063
BciT130I CCWGG 2 cut(s) 110, 771
BciVI GTATCC 1 cut(s) 853
BclI TGATCA 3 cut(s) 307, 538, 1174
BcnI CCSGG 5 cut(s) 347, 361, 506, 1058, 1139
BcoDI GTCTC 3 cut(s) 404, 431, 1061
BfmI CTRYAG 1 cut(s) 905
BfoI RGCGCY 1 cut(s) 567
BfuAI ACCTGC 1 cut(s) 617
BfuI GTATCC 1 cut(s) 853
BglII AGATCT 1 cut(s) 576
BisI GCNGC 2 cut(s) 5, 908
BlpI GCTNAGC 1 cut(s) 741
BlsI GCNGC 2 cut(s) 6, 909
Bme1390I CCNGG 7 cut(s) 110, 347, 361, 506, 771, 1058, 1139
Bme18I GGWCC 2 cut(s) 1091, 1297
BmgT120I GGNCC 2 cut(s) 1091, 1297
BmiI GGNNCC 3 cut(s) 565, 1092, 1143
BmrFI CCNGG 7 cut(s) 110, 347, 361, 506, 771, 1058, 1139
BmsI GCATC 4 cut(s) 363, 832, 859, 1218
BpiI GAAGAC 4 cut(s) 469, 527, 962, 1159
BpmI CTGGAG 1 cut(s) 1125
Bpu1102I GCTNAGC 1 cut(s) 741
Bpu14I TTCGAA 1 cut(s) 206
BpuEI CTTGAG 3 cut(s) 703, 878, 1286
BpuMI CCSGG 5 cut(s) 347, 361, 506, 1058, 1139
BsaHI GRCGYC 2 cut(s) 564, 1050
BsaI GGTCTC 1 cut(s) 404
BsaJI CCNNGG 4 cut(s) 345, 360, 837, 1079
BsaWI WCCGGW 2 cut(s) 402, 1358
Bsc4I CCNNNNNNNGG 3 cut(s) 276, 1358, 1365
Bse118I RCCGGY 1 cut(s) 1358
Bse1I ACTGG 1 cut(s) 1157
BseBI CCWGG 2 cut(s) 110, 771
BseDI CCNNGG 4 cut(s) 345, 360, 837, 1079
BseGI GGATG 3 cut(s) 501, 823, 1201
BseLI CCNNNNNNNGG 3 cut(s) 276, 1358, 1365
BseMII CTCAG 2 cut(s) 585, 732
BseNI ACTGG 1 cut(s) 1157
BseRI GAGGAG 1 cut(s) 1283
BseXI GCAGC 2 cut(s) 16, 919
BsgI GTGCAG 2 cut(s) 64, 645
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 1 cut(s) 810
BshNI GGYRCC 1 cut(s) 563
BshTI ACCGGT 1 cut(s) 1358
BsiHKAI GWGCWC 2 cut(s) 549, 864
BsiSI CCGG 7 cut(s) 347, 360, 403, 506, 1058, 1139, 1359
BslFI GGGAC 3 cut(s) 130, 377, 1104
BslI CCNNNNNNNGG 3 cut(s) 276, 1358, 1365
BsmAI GTCTC 3 cut(s) 404, 431, 1061
BsmFI GGGAC 3 cut(s) 130, 377, 1104
BsmI GAATGC 2 cut(s) 1397, 1420
BsnI GGCC 1 cut(s) 810
Bso31I GGTCTC 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 206
Bsp1286I GDGCHC 2 cut(s) 549, 864
Bsp143I GATC 8 cut(s) 307, 538, 576, 723, 787, 934, 1141, 1174
Bsp1720I GCTNAGC 1 cut(s) 741
BspACI CCGC 1 cut(s) 621
BspANI GGCC 1 cut(s) 810
BspCNI CTCAG 2 cut(s) 584, 733
BspFNI CGCG 1 cut(s) 417
BspHI TCATGA 2 cut(s) 541, 1203
BspLI GGNNCC 3 cut(s) 565, 1092, 1143
BspMAI CTGCAG 1 cut(s) 909
BspMI ACCTGC 1 cut(s) 617
BspPI GGATC 4 cut(s) 731, 929, 1136, 1149
BspT104I TTCGAA 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 563
BspTNI GGTCTC 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 1358
BsrI ACTGG 1 cut(s) 1157
BssAI RCCGGY 1 cut(s) 1358
BssECI CCNNGG 4 cut(s) 345, 360, 837, 1079
BssMI GATC 8 cut(s) 307, 538, 576, 723, 787, 934, 1141, 1174
BssNI GRCGYC 2 cut(s) 564, 1050
BssSI CACGAG 2 cut(s) 423, 764
BssT1I CCWWGG 1 cut(s) 837
Bst2BI CACGAG 2 cut(s) 423, 764
Bst2UI CCWGG 2 cut(s) 110, 771
Bst4CI ACNGT 5 cut(s) 442, 496, 961, 991, 1314
Bst6I CTCTTC 1 cut(s) 345
BstACI GRCGYC 2 cut(s) 564, 1050
BstAPI GCANNNNNTGC 2 cut(s) 632, 904
BstBI TTCGAA 1 cut(s) 206
BstC8I GCNNGC 3 cut(s) 83, 900, 1320
BstDEI CTNAG 3 cut(s) 571, 741, 1425
BstDSI CCRYGG 1 cut(s) 1079
BstF5I GGATG 3 cut(s) 501, 823, 1201
BstFNI CGCG 1 cut(s) 417
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 8 cut(s) 310, 541, 579, 726, 790, 937, 1144, 1177
BstMAI GTCTC 3 cut(s) 404, 431, 1061
BstMBI GATC 8 cut(s) 307, 538, 576, 723, 787, 934, 1141, 1174
BstMWI GCNNNNNNNGC 2 cut(s) 632, 904
BstNI CCWGG 2 cut(s) 110, 771
BstNSI RCATGY 1 cut(s) 1420
BstSCI CCNGG 7 cut(s) 108, 345, 359, 504, 769, 1056, 1137
BstSFI CTRYAG 1 cut(s) 905
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 16, 919
BstV2I GAAGAC 4 cut(s) 469, 527, 962, 1159
BstX2I RGATCY 3 cut(s) 576, 723, 1141
BstYI RGATCY 3 cut(s) 576, 723, 1141
BsuI GTATCC 1 cut(s) 853
BsuRI GGCC 1 cut(s) 810
BtgI CCRYGG 1 cut(s) 1079
BtsCI GGATG 3 cut(s) 501, 823, 1201
BtsI GCAGTG 1 cut(s) 718
BtsIMutI CAGTG 1 cut(s) 718
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 3 cut(s) 83, 900, 1320
CciI TCATGA 2 cut(s) 541, 1203
CfoI GCGC 1 cut(s) 566
Cfr10I RCCGGY 1 cut(s) 1358
Cfr13I GGNCC 2 cut(s) 1091, 1297
Csp6I GTAC 1 cut(s) 553
CspAI ACCGGT 1 cut(s) 1358
CspCI CAANNNNNGTGG 2 cut(s) 399, 434
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 3 cut(s) 571, 741, 1425
DinI GGCGCC 1 cut(s) 565
DpnI GATC 8 cut(s) 309, 540, 578, 725, 789, 936, 1143, 1176
DpnII GATC 8 cut(s) 307, 538, 576, 723, 787, 934, 1141, 1174
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 837
Eco24I GRGCYC 1 cut(s) 549
Eco31I GGTCTC 1 cut(s) 404
Eco47I GGWCC 2 cut(s) 1091, 1297
Eco53kI GAGCTC 1 cut(s) 547
EcoICRI GAGCTC 1 cut(s) 547
EcoO109I RGGNCCY 1 cut(s) 1091
EcoRII CCWGG 2 cut(s) 108, 769
EcoT14I CCWWGG 1 cut(s) 837
EcoT22I ATGCAT 2 cut(s) 356, 1422
EcoT38I GRGCYC 1 cut(s) 549
EgeI GGCGCC 1 cut(s) 565
EheI GGCGCC 1 cut(s) 565
ErhI CCWWGG 1 cut(s) 837
FaqI GGGAC 3 cut(s) 130, 377, 1104
FauNDI CATATG 1 cut(s) 1035
FbaI TGATCA 3 cut(s) 307, 538, 1174
Fnu4HI GCNGC 2 cut(s) 5, 908
FokI GGATG 3 cut(s) 488, 810, 1208
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 2 cut(s) 5, 908
GlaI GCGC 1 cut(s) 565
GluI GCNGC 2 cut(s) 5, 908
GsuI CTGGAG 1 cut(s) 1125
HaeII RGCGCY 1 cut(s) 567
HaeIII GGCC 1 cut(s) 810
HapII CCGG 7 cut(s) 347, 360, 403, 506, 1058, 1139, 1359
HhaI GCGC 1 cut(s) 566
Hin1I GRCGYC 2 cut(s) 564, 1050
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HindIII AAGCTT 1 cut(s) 1262
HinfI GANTC 5 cut(s) 226, 523, 677, 716, 1200
HpaII CCGG 7 cut(s) 347, 360, 403, 506, 1058, 1139, 1359
HphI GGTGA 5 cut(s) 9, 529, 809, 1127, 1381
HpyAV CCTTC 4 cut(s) 229, 308, 1323, 1362
HpyCH4III ACNGT 5 cut(s) 442, 496, 961, 991, 1314
HpyCH4IV ACGT 2 cut(s) 803, 1050
HpyF10VI GCNNNNNNNGC 2 cut(s) 632, 904
HpyF3I CTNAG 3 cut(s) 571, 741, 1425
HpySE526I ACGT 2 cut(s) 803, 1050
Hsp92I GRCGYC 2 cut(s) 564, 1050
HspAI GCGC 1 cut(s) 564
KasI GGCGCC 1 cut(s) 563
Ksp22I TGATCA 3 cut(s) 307, 538, 1174
Kzo9I GATC 8 cut(s) 307, 538, 576, 723, 787, 934, 1141, 1174
LmnI GCTCC 3 cut(s) 128, 1241, 1327
Lsp1109I GCAGC 2 cut(s) 16, 919
LweI GCATC 4 cut(s) 363, 832, 859, 1218
MaeII ACGT 2 cut(s) 803, 1050
MaeIII GTNAC 5 cut(s) 380, 641, 713, 1046, 1340
MalI GATC 8 cut(s) 309, 540, 578, 725, 789, 936, 1143, 1176
MboI GATC 8 cut(s) 307, 538, 576, 723, 787, 934, 1141, 1174
MflI RGATCY 3 cut(s) 576, 723, 1141
MhlI GDGCHC 2 cut(s) 549, 864
MluCI AATT 6 cut(s) 198, 411, 511, 594, 1231, 1406
Mly113I GGCGCC 1 cut(s) 564
MlyI GAGTC 3 cut(s) 235, 517, 710
MnlI CCTC 9 cut(s) 65, 225, 267, 948, 1050, 1064, 1186, 1261, 1264
Mph1103I ATGCAT 2 cut(s) 356, 1422
MroXI GAANNNNTTC 2 cut(s) 202, 297
MseI TTAA 3 cut(s) 758, 1119, 1409
MslI CAYNNNNRTG 5 cut(s) 327, 459, 705, 783, 1338
MspI CCGG 7 cut(s) 347, 360, 403, 506, 1058, 1139, 1359
MspR9I CCNGG 7 cut(s) 110, 347, 361, 506, 771, 1058, 1139
Mva1269I GAATGC 2 cut(s) 1397, 1420
MvaI CCWGG 2 cut(s) 110, 771
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 2 cut(s) 632, 904
NarI GGCGCC 1 cut(s) 564
NciI CCSGG 5 cut(s) 347, 361, 506, 1058, 1139
NdeI CATATG 1 cut(s) 1035
NdeII GATC 8 cut(s) 307, 538, 576, 723, 787, 934, 1141, 1174
NlaIV GGNNCC 3 cut(s) 565, 1092, 1143
NmuCI GTSAC 3 cut(s) 713, 1046, 1340
NsiI ATGCAT 2 cut(s) 356, 1422
NspI RCATGY 1 cut(s) 1420
NspV TTCGAA 1 cut(s) 206
PagI TCATGA 2 cut(s) 541, 1203
PcsI WCGNNNNNNNCGW 2 cut(s) 140, 421
PctI GAATGC 2 cut(s) 1397, 1420
PdmI GAANNNNTTC 2 cut(s) 202, 297
PfeI GAWTC 2 cut(s) 677, 1200
PfoI TCCNGGA 1 cut(s) 504
PinAI ACCGGT 1 cut(s) 1358
PkrI GCNGC 2 cut(s) 6, 909
PleI GAGTC 3 cut(s) 234, 517, 710
PluTI GGCGCC 1 cut(s) 567
PpsI GAGTC 3 cut(s) 234, 517, 710
PpuMI RGGWCCY 1 cut(s) 1091
Psp124BI GAGCTC 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 1091
Psp6I CCWGG 2 cut(s) 108, 769
PspGI CCWGG 2 cut(s) 108, 769
PspN4I GGNNCC 3 cut(s) 565, 1092, 1143
PspPI GGNCC 2 cut(s) 1091, 1297
PspPPI RGGWCCY 1 cut(s) 1091
PstI CTGCAG 1 cut(s) 909
PsuI RGATCY 3 cut(s) 576, 723, 1141
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 5 cut(s) 327, 459, 705, 783, 1338
SacI GAGCTC 1 cut(s) 549
SaqAI TTAA 3 cut(s) 758, 1119, 1409
SatI GCNGC 2 cut(s) 5, 908
Sau3AI GATC 8 cut(s) 307, 538, 576, 723, 787, 934, 1141, 1174
Sau96I GGNCC 2 cut(s) 1091, 1297
SchI GAGTC 3 cut(s) 235, 517, 710
ScrFI CCNGG 7 cut(s) 110, 347, 361, 506, 771, 1058, 1139
SduI GDGCHC 2 cut(s) 549, 864
SfaNI GCATC 4 cut(s) 363, 832, 859, 1218
SfcI CTRYAG 1 cut(s) 905
SfoI GGCGCC 1 cut(s) 565
SfuI TTCGAA 1 cut(s) 206
SinI GGWCC 2 cut(s) 1091, 1297
SmiMI CAYNNNNRTG 5 cut(s) 327, 459, 705, 783, 1338
SmlI CTYRAG 3 cut(s) 718, 857, 1265
SmoI CTYRAG 3 cut(s) 718, 857, 1265
Sse9I AATT 6 cut(s) 198, 411, 511, 594, 1231, 1406
SsiI CCGC 1 cut(s) 621
SspDI GGCGCC 1 cut(s) 563
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 7 cut(s) 108, 345, 359, 504, 769, 1056, 1137
StyI CCWWGG 1 cut(s) 837
TaaI ACNGT 5 cut(s) 442, 496, 961, 991, 1314
TaiI ACGT 2 cut(s) 806, 1053
TaqI TCGA 4 cut(s) 206, 291, 1150, 1187
TasI AATT 6 cut(s) 198, 411, 511, 594, 1231, 1406
TfiI GAWTC 2 cut(s) 677, 1200
Tru1I TTAA 3 cut(s) 758, 1119, 1409
Tru9I TTAA 3 cut(s) 758, 1119, 1409
TscAI CASTG 1 cut(s) 718
TseFI GTSAC 3 cut(s) 713, 1046, 1340
TseI GCWGC 2 cut(s) 4, 907
Tsp45I GTSAC 3 cut(s) 713, 1046, 1340
TspDTI ATGAA 5 cut(s) 490, 669, 767, 1242, 1416
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 1 cut(s) 718
VpaK11BI GGWCC 2 cut(s) 1091, 1297
XapI RAATTY 2 cut(s) 511, 594
XceI RCATGY 1 cut(s) 1420
XmnI GAANNNNTTC 2 cut(s) 202, 297
ZraI GACGTC 1 cut(s) 1051
Zsp2I ATGCAT 2 cut(s) 356, 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.