MD17G1092000.v1.1

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013240
Physical Location & Seq
Reverse (-)
7711238 .. 7713056
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1092000.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGCTGCCGTTTCCTCAGTCGCCTTCGAAACCCCAAAGTCCGTCTCGGTTGATAAACTTCTTCATAGTCTCATCTTCTTTATGCACGCTCTTCCTTCTTGGGTCGCTCTTCCTTGTGTTTACGAGCTCAAAGCTTGGACGTATAAGTTCTCTGTCTCAAGATGCGTATTCCCCGCCCTCGACCTCCCTTGAGCACGTTGTGTTCGGGATTGCTTCGAATCAGAAAGCGTGGTCTGCTAAGAGGAAGGAGTATGTACGGCTCTGGTGGAGGAACCAGTCTATGAGGGGATGTGTCTTTCTCGATAGCCTTCCGCCTGATCATCAGCGTCACGTCCATGACACTTCTCTTCCTCCTGCGTGCATCTCGGGTGACACTGCAAGATTTCGTTACACTTACAGAGGCGGTCTCAAGTCTGCTATTCGTGTTGCACGCGTTGTTTCTGAGACCGTGGCGCTCAACCATACGAATGTGAGGTGGTATGTTTTCGGAGATGATGACACAGTTTTTTTCCCTGAGAATTTGGTGAAGACACTTTCTAAGTATGATCATGGACTGTGGTACTACATTGGGACAAACTCCGAAGTTTATGAGCAAAATAGGGTGTTCGGATTTGGAATGGCTTATGGTGGTGCAGGTTTCGCTATAAGTGCCCCATTGGCGAAAGTACTGGCGAAGGTGTTTGATTCGTGTTTAGAACGATATCCACATCTCTATGGAAGCGACTCTAGGATTTCCTCTTGCTTGGCAGAACTCGGCGTTGGATTAACAAGCGAAGCCGGTTTCCATCAGAATGACATTCACGGCGATATGTTTGGCCTACTGGCTTCGCATCCGGTGACGCCTTTGGTATCCCTGCATCACTTCGATCATGCAAACCCTGTCTTCCCCAACATGACAACTATCAATGCTCTAAAACATTTGTTTAAAGCTGCGAATGTTGATTCTCAAAGAATGTTACAGAAAACAGTGTGCTACGACAGATGGTTTTCGTGGACTATCACGGTGTCATGGGGTTACGCTGTTCAAGTATACGGAAATCATATTCTTCTGCCGGACGTCCTCCCCGTGCAGCAGACATTCAGCCAGTGGAAAAAGGGAAGTGTTTTGTCAGGAGTTTATACTTTTGACACAAGAGAACATCACAAGGATCCGTGTCGAAGGCCGGTTGTTTTCTTTCTGGATAATGTGTCTTCTGGTGCGGATGGAATCAAGAGCATATACAAAAAGTCCTACGAAAATTGCTCCTACGACATGGCTTCGCCGAGGAAGCTGGAGGAGGTCAGAGTGTACTCACATAAGCTGGACCTTGATATGAAACAGGCGCCGCGAAGGCAATGTTGCGATGTATTACCTTCTACGGGCAGCAAATTGATGGAAATCGAAATCAGAGAATGCAAAGAAGAAGAACTAATACGCATGCATCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

476

Amino Acids

53.78

Weight (kDa)

8.73

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 140 - 264 2e-11 Fringe-like
DUF604 PF04646 198 - 447 1.3e-106 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05280 AT1G05280
fragaria_vesca FvH4_6g43600
malus_domestica MD09G1104300.v1.1 MD17G1092000.v1.1
prunus_persica Prupe.3G221300_v2.0.a1
pyrus_communis pycom09g02750 pycom17g08910
rosa_chinensis RchiOBHm_Chr2g0160191
rosa_laevigata RLG00000021203
rosa_multiflora Rmu_sc0000403.1_g000007
rosa_roxburghii Rroxscaffold_2G00089970
rosa_rugosa Rorug02G0482300
rosa_samantha Rh2AG547700 Rh2BG560900 Rh2CG531500 Rh2DG569600
rosa_wichuraiana Rw2G045290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1059
Acc36I ACCTGC 1 cut(s) 623
AccB1I GGYRCC 1 cut(s) 1321
AccI GTMKAC 1 cut(s) 1029
AccII CGCG 2 cut(s) 432, 1327
AciI CCGC 5 cut(s) 173, 311, 402, 1199, 1325
AclWI GGATC 2 cut(s) 1142, 1155
AcsI RAATTY 1 cut(s) 517
AcyI GRCGYC 3 cut(s) 839, 1056, 1322
AdeI CACNNNGTG 1 cut(s) 199
AfaI GTAC 4 cut(s) 255, 560, 666, 1289
AfiI CCNNNNNNNGG 1 cut(s) 1358
AflIII ACRYGT 1 cut(s) 430
AgsI TTSAA 1 cut(s) 1025
AjiI CACGTC 1 cut(s) 331
AjuI GAANNNNNNNTTGG 2 cut(s) 741, 773
AluBI AGCT 5 cut(s) 126, 133, 929, 1270, 1300
AluI AGCT 5 cut(s) 126, 133, 929, 1270, 1300
Alw21I GWGCWC 2 cut(s) 128, 195
Alw26I GTCTC 5 cut(s) 48, 73, 159, 410, 437
AlwI GGATC 2 cut(s) 1142, 1155
Ama87I CYCGRG 1 cut(s) 364
AoxI GGCC 2 cut(s) 814, 1160
ApeKI GCWGC 4 cut(s) 4, 929, 1069, 1362
ApoI RAATTY 1 cut(s) 517
AspLEI GCGC 2 cut(s) 454, 1324
AspS9I GGNCC 1 cut(s) 1303
AsuHPI GGTGA 3 cut(s) 380, 535, 847
AsuII TTCGAA 2 cut(s) 26, 215
AvaI CYCGRG 1 cut(s) 364
AvaII GGWCC 1 cut(s) 1303
BaeGI GKGCMC 1 cut(s) 652
BamHI GGATCC 1 cut(s) 1147
BanI GGYRCC 1 cut(s) 1321
BanII GRGCYC 1 cut(s) 128
BarI GAAGNNNNNNTAC 2 cut(s) 1395, 1427
BbsI GAAGAC 3 cut(s) 533, 874, 1182
Bbv12I GWGCWC 2 cut(s) 128, 195
BbvI GCAGC 3 cut(s) 916, 1081, 1374
BccI CCATC 4 cut(s) 792, 975, 1196, 1366
BceAI ACGGC 2 cut(s) 272, 817
BciVI GTATCC 1 cut(s) 859
BclI TGATCA 2 cut(s) 316, 544
BcoDI GTCTC 5 cut(s) 48, 73, 159, 410, 437
BfaI CTAG 1 cut(s) 726
BfoI RGCGCY 2 cut(s) 455, 1325
BfuAI ACCTGC 1 cut(s) 623
BfuI GTATCC 1 cut(s) 859
BglI GCCNNNNNGGC 2 cut(s) 656, 1330
BisI GCNGC 5 cut(s) 5, 930, 1070, 1325, 1363
BlsI GCNGC 5 cut(s) 6, 931, 1071, 1326, 1364
BmcAI AGTACT 1 cut(s) 666
Bme18I GGWCC 1 cut(s) 1303
BmeT110I CYCGRG 1 cut(s) 364
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 1 cut(s) 1303
BmiI GGNNCC 3 cut(s) 272, 1149, 1323
BmsI GCATC 4 cut(s) 151, 369, 838, 865
BpiI GAAGAC 3 cut(s) 533, 874, 1182
BplI GAGNNNNNCTC 2 cut(s) 390, 422
BpmI CTGGAG 1 cut(s) 1292
Bpu14I TTCGAA 2 cut(s) 26, 215
BpuEI CTTGAG 3 cut(s) 141, 209, 392
BsaBI GATNNNNATC 1 cut(s) 1376
BsaHI GRCGYC 3 cut(s) 839, 1056, 1322
BsaI GGTCTC 2 cut(s) 410, 437
BsaJI CCNNGG 2 cut(s) 447, 1262
BsaWI WCCGGW 1 cut(s) 832
Bsc4I CCNNNNNNNGG 1 cut(s) 1358
Bse118I RCCGGY 2 cut(s) 776, 1162
Bse1I ACTGG 4 cut(s) 274, 672, 825, 1084
Bse3DI GCAATG 1 cut(s) 1340
Bse8I GATNNNNATC 1 cut(s) 1376
BseDI CCNNGG 2 cut(s) 447, 1262
BseGI GGATG 4 cut(s) 293, 829, 1207, 1420
BseJI GATNNNNATC 1 cut(s) 1376
BseLI CCNNNNNNNGG 1 cut(s) 1358
BseMI GCAATG 1 cut(s) 1340
BseMII CTCAG 3 cut(s) 29, 432, 504
BseNI ACTGG 4 cut(s) 274, 672, 825, 1084
BseRI GAGGAG 1 cut(s) 1289
BseSI GKGCMC 1 cut(s) 652
BseXI GCAGC 3 cut(s) 916, 1081, 1374
BsgI GTGCAG 2 cut(s) 651, 1088
Bsh1236I CGCG 2 cut(s) 432, 1327
BshFI GGCC 2 cut(s) 816, 1162
BshNI GGYRCC 1 cut(s) 1321
BsiHKAI GWGCWC 2 cut(s) 128, 195
BsiHKCI CYCGRG 1 cut(s) 364
BsiSI CCGG 4 cut(s) 777, 833, 1052, 1163
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 1 cut(s) 1358
BsmAI GTCTC 5 cut(s) 48, 73, 159, 410, 437
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 583
BsmI GAATGC 1 cut(s) 1397
BsnI GGCC 2 cut(s) 816, 1162
Bso31I GGTCTC 2 cut(s) 410, 437
BsoBI CYCGRG 1 cut(s) 364
Bsp119I TTCGAA 2 cut(s) 26, 215
Bsp1286I GDGCHC 3 cut(s) 128, 195, 652
Bsp143I GATC 4 cut(s) 316, 544, 865, 1147
BspACI CCGC 5 cut(s) 173, 311, 402, 1199, 1325
BspANI GGCC 2 cut(s) 816, 1162
BspCNI CTCAG 3 cut(s) 28, 433, 505
BspFNI CGCG 2 cut(s) 432, 1327
BspLI GGNNCC 3 cut(s) 272, 1149, 1323
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 2 cut(s) 1142, 1155
BspQI GCTCTTC 2 cut(s) 95, 113
BspT104I TTCGAA 2 cut(s) 26, 215
BspT107I GGYRCC 1 cut(s) 1321
BspTNI GGTCTC 2 cut(s) 410, 437
BsrDI GCAATG 1 cut(s) 1340
BsrFI RCCGGY 2 cut(s) 776, 1162
BsrI ACTGG 4 cut(s) 274, 672, 825, 1084
BssAI RCCGGY 2 cut(s) 776, 1162
BssECI CCNNGG 2 cut(s) 447, 1262
BssMI GATC 4 cut(s) 316, 544, 865, 1147
BssNAI GTATAC 1 cut(s) 1030
BssNI GRCGYC 3 cut(s) 839, 1056, 1322
Bst1107I GTATAC 1 cut(s) 1030
Bst4CI ACNGT 5 cut(s) 448, 502, 555, 967, 1003
Bst6I CTCTTC 3 cut(s) 95, 113, 351
BstACI GRCGYC 3 cut(s) 839, 1056, 1322
BstBI TTCGAA 2 cut(s) 26, 215
BstC8I GCNNGC 4 cut(s) 86, 358, 430, 1418
BstDEI CTNAG 5 cut(s) 15, 237, 441, 513, 537
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 4 cut(s) 293, 829, 1207, 1420
BstFNI CGCG 2 cut(s) 432, 1327
BstH2I RGCGCY 2 cut(s) 455, 1325
BstHHI GCGC 2 cut(s) 454, 1324
BstKTI GATC 4 cut(s) 319, 547, 868, 1150
BstMAI GTCTC 5 cut(s) 48, 73, 159, 410, 437
BstMBI GATC 4 cut(s) 316, 544, 865, 1147
BstMWI GCNNNNNNNGC 6 cut(s) 233, 638, 647, 656, 1267, 1330
BstNSI RCATGY 1 cut(s) 1420
BstSLI GKGCMC 1 cut(s) 652
BstUI CGCG 2 cut(s) 432, 1327
BstV1I GCAGC 3 cut(s) 916, 1081, 1374
BstV2I GAAGAC 3 cut(s) 533, 874, 1182
BstX2I RGATCY 1 cut(s) 1147
BstYI RGATCY 1 cut(s) 1147
BstZ17I GTATAC 1 cut(s) 1030
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 2 cut(s) 816, 1162
BtgI CCRYGG 1 cut(s) 447
BtgZI GCGATG 1 cut(s) 1356
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 4 cut(s) 293, 829, 1207, 1420
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 3 cut(s) 372, 972, 1091
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 4 cut(s) 86, 358, 430, 1418
CfoI GCGC 2 cut(s) 454, 1324
Cfr10I RCCGGY 2 cut(s) 776, 1162
Cfr13I GGNCC 1 cut(s) 1303
CseI GACGC 2 cut(s) 314, 847
Csp6I GTAC 4 cut(s) 254, 559, 665, 1288
CviAII CATG 7 cut(s) 335, 548, 869, 892, 1008, 1252, 1417
CviQI GTAC 4 cut(s) 254, 559, 665, 1288
DdeI CTNAG 5 cut(s) 15, 237, 441, 513, 537
DinI GGCGCC 1 cut(s) 1323
DpnI GATC 4 cut(s) 318, 546, 867, 1149
DpnII GATC 4 cut(s) 316, 544, 865, 1147
DraI TTTAAA 1 cut(s) 925
DraIII CACNNNGTG 1 cut(s) 199
Eam1104I CTCTTC 3 cut(s) 95, 113, 351
EarI CTCTTC 3 cut(s) 95, 113, 351
EciI GGCGGA 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 128
Eco31I GGTCTC 2 cut(s) 410, 437
Eco32I GATATC 1 cut(s) 701
Eco47I GGWCC 1 cut(s) 1303
Eco53kI GAGCTC 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 364
EcoICRI GAGCTC 1 cut(s) 126
EcoRV GATATC 1 cut(s) 701
EcoT22I ATGCAT 1 cut(s) 1422
EcoT38I GRGCYC 1 cut(s) 128
EgeI GGCGCC 1 cut(s) 1323
EheI GGCGCC 1 cut(s) 1323
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 7 cut(s) 338, 551, 872, 895, 1011, 1255, 1420
FaqI GGGAC 1 cut(s) 583
FatI CATG 7 cut(s) 334, 547, 868, 891, 1007, 1251, 1416
FauI CCCGC 1 cut(s) 180
FbaI TGATCA 2 cut(s) 316, 544
FblI GTMKAC 1 cut(s) 1029
Fnu4HI GCNGC 5 cut(s) 5, 930, 1070, 1325, 1363
FokI GGATG 4 cut(s) 300, 816, 1214, 1407
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 5 cut(s) 5, 930, 1070, 1325, 1363
FspBI CTAG 1 cut(s) 726
GlaI GCGC 2 cut(s) 453, 1323
GluI GCNGC 5 cut(s) 5, 930, 1070, 1325, 1363
GsuI CTGGAG 1 cut(s) 1292
HaeII RGCGCY 2 cut(s) 455, 1325
HaeIII GGCC 2 cut(s) 816, 1162
HapII CCGG 4 cut(s) 777, 833, 1052, 1163
HgaI GACGC 2 cut(s) 314, 847
HhaI GCGC 2 cut(s) 454, 1324
Hin1I GRCGYC 3 cut(s) 839, 1056, 1322
Hin1II CATG 7 cut(s) 338, 551, 872, 895, 1011, 1255, 1420
Hin6I GCGC 2 cut(s) 452, 1322
HinP1I GCGC 2 cut(s) 452, 1322
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 5 cut(s) 217, 683, 722, 941, 1206
HpaII CCGG 4 cut(s) 777, 833, 1052, 1163
HphI GGTGA 3 cut(s) 380, 535, 847
Hpy166II GTNNAC 4 cut(s) 120, 993, 1030, 1288
Hpy188I TCNGA 8 cut(s) 222, 442, 488, 580, 608, 789, 1283, 1388
Hpy188III TCNNGA 6 cut(s) 158, 205, 299, 1110, 1178, 1210
Hpy8I GTNNAC 4 cut(s) 120, 993, 1030, 1288
HpyAV CCTTC 8 cut(s) 33, 104, 238, 317, 667, 1152, 1323, 1362
HpyCH4III ACNGT 5 cut(s) 448, 502, 555, 967, 1003
HpyCH4IV ACGT 4 cut(s) 139, 195, 330, 1056
HpyF10VI GCNNNNNNNGC 6 cut(s) 233, 638, 647, 656, 1267, 1330
HpyF3I CTNAG 5 cut(s) 15, 237, 441, 513, 537
HpySE526I ACGT 4 cut(s) 139, 195, 330, 1056
Hsp92I GRCGYC 3 cut(s) 839, 1056, 1322
Hsp92II CATG 7 cut(s) 338, 551, 872, 895, 1011, 1255, 1420
HspAI GCGC 2 cut(s) 452, 1322
KasI GGCGCC 1 cut(s) 1321
Ksp22I TGATCA 2 cut(s) 316, 544
Kzo9I GATC 4 cut(s) 316, 544, 865, 1147
LguI GCTCTTC 2 cut(s) 95, 113
LmnI GCTCC 1 cut(s) 1247
Lsp1109I GCAGC 3 cut(s) 916, 1081, 1374
LweI GCATC 4 cut(s) 151, 369, 838, 865
MaeI CTAG 1 cut(s) 726
MaeII ACGT 4 cut(s) 139, 195, 330, 1056
MaeIII GTNAC 6 cut(s) 326, 368, 386, 835, 954, 1013
MalI GATC 4 cut(s) 318, 546, 867, 1149
MboI GATC 4 cut(s) 316, 544, 865, 1147
MflI RGATCY 1 cut(s) 1147
MhlI GDGCHC 3 cut(s) 128, 195, 652
MluCI AATT 3 cut(s) 517, 1237, 1367
MluI ACGCGT 1 cut(s) 430
Mly113I GGCGCC 1 cut(s) 1322
MlyI GAGTC 1 cut(s) 716
MmeI TCCRAC 1 cut(s) 739
Mph1103I ATGCAT 1 cut(s) 1422
MseI TTAA 2 cut(s) 764, 924
MslI CAYNNNNRTG 4 cut(s) 333, 465, 711, 789
MspI CCGG 4 cut(s) 777, 833, 1052, 1163
Mva1269I GAATGC 1 cut(s) 1397
MvnI CGCG 2 cut(s) 432, 1327
MwoI GCNNNNNNNGC 6 cut(s) 233, 638, 647, 656, 1267, 1330
NarI GGCGCC 1 cut(s) 1322
NdeII GATC 4 cut(s) 316, 544, 865, 1147
NlaIII CATG 7 cut(s) 338, 551, 872, 895, 1011, 1255, 1420
NlaIV GGNNCC 3 cut(s) 272, 1149, 1323
NmeAIII GCCGAG 2 cut(s) 732, 1287
NmuCI GTSAC 3 cut(s) 326, 368, 835
NsiI ATGCAT 1 cut(s) 1422
NspI RCATGY 1 cut(s) 1420
NspV TTCGAA 2 cut(s) 26, 215
PaeI GCATGC 1 cut(s) 1420
PciSI GCTCTTC 2 cut(s) 95, 113
PcsI WCGNNNNNNNCGW 1 cut(s) 1062
PctI GAATGC 1 cut(s) 1397
PfeI GAWTC 4 cut(s) 217, 683, 941, 1206
PkrI GCNGC 5 cut(s) 6, 931, 1071, 1326, 1364
PleI GAGTC 1 cut(s) 716
PluTI GGCGCC 1 cut(s) 1325
PpsI GAGTC 1 cut(s) 716
Psp124BI GAGCTC 1 cut(s) 128
PspN4I GGNNCC 3 cut(s) 272, 1149, 1323
PspPI GGNCC 1 cut(s) 1303
PsuI RGATCY 1 cut(s) 1147
RsaI GTAC 4 cut(s) 255, 560, 666, 1289
RsaNI GTAC 4 cut(s) 254, 559, 665, 1288
RseI CAYNNNNRTG 4 cut(s) 333, 465, 711, 789
SacI GAGCTC 1 cut(s) 128
SapI GCTCTTC 2 cut(s) 95, 113
SaqAI TTAA 2 cut(s) 764, 924
SatI GCNGC 5 cut(s) 5, 930, 1070, 1325, 1363
Sau3AI GATC 4 cut(s) 316, 544, 865, 1147
Sau96I GGNCC 1 cut(s) 1303
ScaI AGTACT 1 cut(s) 666
SchI GAGTC 1 cut(s) 716
SduI GDGCHC 3 cut(s) 128, 195, 652
SfaNI GCATC 4 cut(s) 151, 369, 838, 865
SfoI GGCGCC 1 cut(s) 1323
SfuI TTCGAA 2 cut(s) 26, 215
SinI GGWCC 1 cut(s) 1303
SmiMI CAYNNNNRTG 4 cut(s) 333, 465, 711, 789
SmlI CTYRAG 3 cut(s) 156, 188, 407
SmoI CTYRAG 3 cut(s) 156, 188, 407
SphI GCATGC 1 cut(s) 1420
Sse9I AATT 3 cut(s) 517, 1237, 1367
SsiI CCGC 5 cut(s) 173, 311, 402, 1199, 1325
SspDI GGCGCC 1 cut(s) 1321
SspMI CTAG 1 cut(s) 726
SstI GAGCTC 1 cut(s) 128
TaaI ACNGT 5 cut(s) 448, 502, 555, 967, 1003
TaiI ACGT 4 cut(s) 142, 198, 333, 1059
TaqI TCGA 7 cut(s) 26, 179, 215, 300, 864, 1156, 1380
TasI AATT 3 cut(s) 517, 1237, 1367
TatI WGTACW 2 cut(s) 664, 1287
TauI GCSGC 1 cut(s) 1327
TfiI GAWTC 4 cut(s) 217, 683, 941, 1206
Tru1I TTAA 2 cut(s) 764, 924
Tru9I TTAA 2 cut(s) 764, 924
TscAI CASTG 3 cut(s) 379, 972, 1091
TseFI GTSAC 3 cut(s) 326, 368, 835
TseI GCWGC 4 cut(s) 4, 929, 1069, 1362
Tsp45I GTSAC 3 cut(s) 326, 368, 835
TspDTI ATGAA 2 cut(s) 52, 1328
TspGWI ACGGA 4 cut(s) 30, 1047, 1140, 1413
TspRI CASTG 3 cut(s) 379, 972, 1091
VpaK11BI GGWCC 1 cut(s) 1303
XapI RAATTY 1 cut(s) 517
XceI RCATGY 1 cut(s) 1420
XmiI GTMKAC 1 cut(s) 1029
XspI CTAG 1 cut(s) 726
ZraI GACGTC 1 cut(s) 1057
ZrmI AGTACT 1 cut(s) 666
Zsp2I ATGCAT 1 cut(s) 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.