RLG00000021203

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013240
Physical Location & Seq
Forward (+)
72920038 .. 72923023
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021203

Sequence Viewer

Length: 1587 bp
ATGAGTTCTGTGAAAAGAATAAAACTAAATGTGTTGGGAGTCTCATGTCGAAAGGAAGCTACTCCATTATCTGGGAAATTTCATGAACAGAGAAAAAGGAAGAAAAACAGCAGCCACCTGTCTGCCAGTGGATGGATGGAGATTAGCCCAAGTAAATGTGGAAATCTTATTCCGTCACCCACCAAAACCCAAGTTTCTTCTCGCTTGATAAACCTCTACATAATCTCATCTTCTGCCTGCACGCTCTTTCTTTTCGTATCATTCTTCCTGGTGCTTACAAGCCCCAAGAATTCGTATATTTCGTCCCAAGATGCATATGCGACAACTCCTACTTCTCTTGACCATGTTGTGTTCGGAATTGCTTCGAACCAGAAATCATGGACGAGTCAGAGGAAGGAGTATGTTAGGCTCTGGTGGAAGAACCATTCCATGAGGGGGTGTGTTTTTCTCGAAAGCCTTCCACCTGATCATCAACACAATCATACCAATGATACTACTCTTCCTCCGGTATGCATCTCCGGGGACACTTCCAGATTTCGTTACACTTACAAAGGTGGTCTCCGGTCTGCAATTCGCGTGGCACGAGTTGTTTCCGAGACTGTGGCACTCAATCACTCCAATGTGCGCTGGTATGTCTTCGGAGATGATGACACGGTTTTCATCCCGGAGAATTTGGTAAAGACTCTTTCCAAGTATGATCATGAGCTCTGGTATTACATTGGGGCTAACTCAGAGATCTATGAGCAGAACAGAATTTTCGGATTTGGGATGGCTTTTGGTGGTGCAGGTTTTGCTATAAGTTACCCTTTGGCAAAAGCACTGGCTAAGGTGTTTGATTCATGTATAGAAAGATACCCACATCTCTATGGAAGTGACTCGAGGATCTCATCTTGCTTGGCTGAGCTTGGCGTTGGCTTAACTCACGAGCCAGGTTTTCATCAGAATGATCTTCGTGGTGATACGTTTGGTCTTTTGGCTTCGCATCCATTAGCACCCTTGGTATCCCTGCATCATCTTGAGCACACAGACCCTATCTTCCCCAACAGGACAACCATGCAAGCTCTGCAGCATTTATTCAAAGCTGTGAATGTTGATCCTCATAGGATTTTACAGAAGACAGTTTGCTATGATAGATGGTTCTCATGGACAGTTTCGGTGTCATGGGGTTATGCTGTTCAGATATACGGAAACCATATGCCTCTGCGTGACGTCCTCCCGGTGCAAGAGACATTCAAGCCGTGGAAAAAGGGACCTGTTTTGTCCGGAGTTTATACTTTTAATACAAGAGAACATCACCCGGATCCCTGTCGAAGACCAGTTGTTTTCTTTCTTGATCATGTGTCATCGAGAGAGGATGGAATCATGAGCATCTATAAGAAGTCTTATGAAAATTGCTCCTATGACATGGCTTCCCCCAGGAAGCTTGAGGAGGTCAGAGTGCTATCACAAAAGCTGGACCTTGACATCAAACAGTTGCAAGCTCCAAGAAGGCATTGTTGTGACATATTACCTTCTACTGGTGGCGGGGTGATGGACATTGTAATCAGAGAATGCAAACATGAAGAATTAATATACATGCATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

529

Amino Acids

60.03

Weight (kDa)

8.99

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 189 - 315 3.7e-13 Fringe-like
DUF604 PF04646 249 - 500 7.6e-108 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05280 AT1G05280
fragaria_vesca FvH4_6g43600
malus_domestica MD09G1104300.v1.1 MD17G1092000.v1.1
prunus_persica Prupe.3G221300_v2.0.a1
pyrus_communis pycom09g02750 pycom17g08910
rosa_chinensis RchiOBHm_Chr2g0160191
rosa_laevigata RLG00000021203
rosa_multiflora Rmu_sc0000403.1_g000007
rosa_roxburghii Rroxscaffold_2G00089970
rosa_rugosa Rorug02G0482300
rosa_samantha Rh2AG547700 Rh2BG560900 Rh2CG531500 Rh2DG569600
rosa_wichuraiana Rw2G045290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1212
Acc36I ACCTGC 1 cut(s) 776
AccB7I CCANNNNNTGG 2 cut(s) 71, 132
AccII CGCG 1 cut(s) 576
AccIII TCCGGA 1 cut(s) 1262
AciI CCGC 1 cut(s) 1524
AclWI GGATC 4 cut(s) 890, 1088, 1295, 1308
AcsI RAATTY 4 cut(s) 77, 289, 670, 753
AcyI GRCGYC 1 cut(s) 1209
AfiI CCNNNNNNNGG 4 cut(s) 71, 132, 435, 1517
AgsI TTSAA 2 cut(s) 1078, 1234
AjnI CCWGG 3 cut(s) 267, 928, 1415
AluBI AGCT 8 cut(s) 59, 706, 904, 1061, 1082, 1423, 1453, 1481
AluI AGCT 8 cut(s) 59, 706, 904, 1061, 1082, 1423, 1453, 1481
Alw21I GWGCWC 2 cut(s) 708, 1023
Alw26I GTCTC 4 cut(s) 46, 563, 590, 1220
AlwI GGATC 4 cut(s) 890, 1088, 1295, 1308
Ama87I CYCGRG 1 cut(s) 877
Aor13HI TCCGGA 1 cut(s) 1262
ApeKI GCWGC 2 cut(s) 111, 1066
ApoI RAATTY 4 cut(s) 77, 289, 670, 753
AseI ATTAAT 1 cut(s) 1568
Asp700I GAANNNNTTC 2 cut(s) 361, 456
AspLEI GCGC 1 cut(s) 627
AspS9I GGNCC 2 cut(s) 1250, 1456
AsuC2I CCSGG 4 cut(s) 520, 665, 1217, 1298
AsuHPI GGTGA 4 cut(s) 168, 968, 1286, 1540
AsuII TTCGAA 1 cut(s) 365
AvaI CYCGRG 1 cut(s) 877
AvaII GGWCC 2 cut(s) 1250, 1456
BamHI GGATCC 1 cut(s) 1300
BanII GRGCYC 1 cut(s) 708
BauI CACGAG 2 cut(s) 582, 923
BbsI GAAGAC 3 cut(s) 628, 1121, 1318
Bbv12I GWGCWC 2 cut(s) 708, 1023
BbvI GCAGC 2 cut(s) 123, 1078
BccI CCATC 6 cut(s) 126, 130, 763, 1128, 1349, 1525
BceAI ACGGC 1 cut(s) 1222
BciT130I CCWGG 3 cut(s) 269, 930, 1417
BciVI GTATCC 1 cut(s) 1012
BclI TGATCA 3 cut(s) 466, 697, 1333
BcnI CCSGG 4 cut(s) 520, 665, 1217, 1298
BcoDI GTCTC 4 cut(s) 46, 563, 590, 1220
BfmI CTRYAG 1 cut(s) 1064
BfuAI ACCTGC 1 cut(s) 776
BfuI GTATCC 1 cut(s) 1012
BglII AGATCT 1 cut(s) 735
BisI GCNGC 2 cut(s) 112, 1067
BlpI GCTNAGC 1 cut(s) 900
BlsI GCNGC 2 cut(s) 113, 1068
Bme1390I CCNGG 7 cut(s) 269, 520, 665, 930, 1217, 1298, 1417
Bme18I GGWCC 2 cut(s) 1250, 1456
BmeT110I CYCGRG 1 cut(s) 877
BmgT120I GGNCC 2 cut(s) 1250, 1456
BmiI GGNNCC 2 cut(s) 1251, 1302
BmrFI CCNGG 7 cut(s) 269, 520, 665, 930, 1217, 1298, 1417
BmsI GCATC 5 cut(s) 301, 522, 991, 1018, 1377
BpiI GAAGAC 3 cut(s) 628, 1121, 1318
Bpu10I CCTNAGC 1 cut(s) 825
Bpu1102I GCTNAGC 1 cut(s) 900
Bpu14I TTCGAA 1 cut(s) 365
BpuEI CTTGAG 2 cut(s) 1037, 1445
BpuMI CCSGG 4 cut(s) 520, 665, 1217, 1298
BsaHI GRCGYC 1 cut(s) 1209
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 4 cut(s) 519, 996, 1238, 1415
BsaWI WCCGGW 3 cut(s) 505, 561, 1262
BsaXI ACNNNNNCTCC 2 cut(s) 487, 517
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 132, 435, 1517
Bse1I ACTGG 4 cut(s) 126, 825, 1316, 1522
BseAI TCCGGA 1 cut(s) 1262
BseBI CCWGG 3 cut(s) 269, 930, 1417
BseDI CCNNGG 4 cut(s) 519, 996, 1238, 1415
BseGI GGATG 6 cut(s) 137, 141, 660, 774, 982, 1360
BseLI CCNNNNNNNGG 4 cut(s) 71, 132, 435, 1517
BseMII CTCAG 2 cut(s) 744, 891
BseNI ACTGG 4 cut(s) 126, 825, 1316, 1522
BseRI GAGGAG 1 cut(s) 1442
BseXI GCAGC 2 cut(s) 123, 1078
BsgI GTGCAG 2 cut(s) 223, 804
Bsh1236I CGCG 1 cut(s) 576
BsiHKAI GWGCWC 2 cut(s) 708, 1023
BsiHKCI CYCGRG 1 cut(s) 877
BsiSI CCGG 7 cut(s) 506, 519, 562, 665, 1217, 1263, 1298
BslFI GGGAC 3 cut(s) 289, 536, 1263
BslI CCNNNNNNNGG 4 cut(s) 71, 132, 435, 1517
BsmAI GTCTC 4 cut(s) 46, 563, 590, 1220
BsmFI GGGAC 3 cut(s) 289, 536, 1263
BsmI GAATGC 2 cut(s) 1556, 1579
Bso31I GGTCTC 1 cut(s) 563
BsoBI CYCGRG 1 cut(s) 877
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 708, 1023
Bsp13I TCCGGA 1 cut(s) 1262
Bsp143I GATC 8 cut(s) 466, 697, 735, 882, 946, 1093, 1300, 1333
Bsp1720I GCTNAGC 1 cut(s) 900
BspACI CCGC 1 cut(s) 1524
BspCNI CTCAG 2 cut(s) 743, 892
BspEI TCCGGA 1 cut(s) 1262
BspFNI CGCG 1 cut(s) 576
BspHI TCATGA 3 cut(s) 82, 700, 1362
BspLI GGNNCC 2 cut(s) 1251, 1302
BspMAI CTGCAG 1 cut(s) 1068
BspMI ACCTGC 1 cut(s) 776
BspPI GGATC 4 cut(s) 890, 1088, 1295, 1308
BspT104I TTCGAA 1 cut(s) 365
BspTNI GGTCTC 1 cut(s) 563
BsrI ACTGG 4 cut(s) 126, 825, 1316, 1522
BssECI CCNNGG 4 cut(s) 519, 996, 1238, 1415
BssMI GATC 8 cut(s) 466, 697, 735, 882, 946, 1093, 1300, 1333
BssNI GRCGYC 1 cut(s) 1209
BssSI CACGAG 2 cut(s) 582, 923
BssT1I CCWWGG 1 cut(s) 996
Bst2BI CACGAG 2 cut(s) 582, 923
Bst2UI CCWGG 3 cut(s) 269, 930, 1417
Bst4CI ACNGT 5 cut(s) 601, 655, 1120, 1150, 1473
Bst6I CTCTTC 1 cut(s) 504
BstACI GRCGYC 1 cut(s) 1209
BstAPI GCANNNNNTGC 2 cut(s) 791, 1063
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 4 cut(s) 238, 242, 1059, 1479
BstDEI CTNAG 4 cut(s) 730, 825, 900, 1584
BstDSI CCRYGG 1 cut(s) 1238
BstF5I GGATG 6 cut(s) 137, 141, 660, 774, 982, 1360
BstFNI CGCG 1 cut(s) 576
BstHHI GCGC 1 cut(s) 627
BstKTI GATC 8 cut(s) 469, 700, 738, 885, 949, 1096, 1303, 1336
BstMAI GTCTC 4 cut(s) 46, 563, 590, 1220
BstMBI GATC 8 cut(s) 466, 697, 735, 882, 946, 1093, 1300, 1333
BstMWI GCNNNNNNNGC 2 cut(s) 791, 1063
BstNI CCWGG 3 cut(s) 269, 930, 1417
BstNSI RCATGY 1 cut(s) 1579
BstSCI CCNGG 7 cut(s) 267, 518, 663, 928, 1215, 1296, 1415
BstSFI CTRYAG 1 cut(s) 1064
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 2 cut(s) 123, 1078
BstV2I GAAGAC 3 cut(s) 628, 1121, 1318
BstX2I RGATCY 3 cut(s) 735, 882, 1300
BstYI RGATCY 3 cut(s) 735, 882, 1300
BsuI GTATCC 1 cut(s) 1012
BtgI CCRYGG 1 cut(s) 1238
BtsCI GGATG 6 cut(s) 137, 141, 660, 774, 982, 1360
BtsIMutI CAGTG 2 cut(s) 133, 818
BveI ACCTGC 1 cut(s) 776
Cac8I GCNNGC 4 cut(s) 238, 242, 1059, 1479
CciI TCATGA 3 cut(s) 82, 700, 1362
CfoI GCGC 1 cut(s) 627
Cfr13I GGNCC 2 cut(s) 1250, 1456
CspCI CAANNNNNGTGG 2 cut(s) 558, 593
DdeI CTNAG 4 cut(s) 730, 825, 900, 1584
DpnI GATC 8 cut(s) 468, 699, 737, 884, 948, 1095, 1302, 1335
DpnII GATC 8 cut(s) 466, 697, 735, 882, 946, 1093, 1300, 1333
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
Ecl136II GAGCTC 1 cut(s) 706
Eco130I CCWWGG 1 cut(s) 996
Eco24I GRGCYC 1 cut(s) 708
Eco31I GGTCTC 1 cut(s) 563
Eco47I GGWCC 2 cut(s) 1250, 1456
Eco53kI GAGCTC 1 cut(s) 706
Eco88I CYCGRG 1 cut(s) 877
EcoICRI GAGCTC 1 cut(s) 706
EcoO109I RGGNCCY 1 cut(s) 1250
EcoRI GAATTC 1 cut(s) 289
EcoRII CCWGG 3 cut(s) 267, 928, 1415
EcoT14I CCWWGG 1 cut(s) 996
EcoT22I ATGCAT 3 cut(s) 316, 515, 1581
EcoT38I GRGCYC 1 cut(s) 708
ErhI CCWWGG 1 cut(s) 996
FalI AAGNNNNNCTT 2 cut(s) 790, 822
FaqI GGGAC 3 cut(s) 289, 536, 1263
FauI CCCGC 1 cut(s) 1517
FauNDI CATATG 2 cut(s) 316, 1194
FbaI TGATCA 3 cut(s) 466, 697, 1333
Fnu4HI GCNGC 2 cut(s) 112, 1067
FokI GGATG 6 cut(s) 144, 148, 647, 781, 969, 1367
FriOI GRGCYC 1 cut(s) 708
Fsp4HI GCNGC 2 cut(s) 112, 1067
GlaI GCGC 1 cut(s) 626
GluI GCNGC 2 cut(s) 112, 1067
HapII CCGG 7 cut(s) 506, 519, 562, 665, 1217, 1263, 1298
HhaI GCGC 1 cut(s) 627
Hin1I GRCGYC 1 cut(s) 1209
Hin6I GCGC 1 cut(s) 625
HinP1I GCGC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 1421
HinfI GANTC 6 cut(s) 39, 385, 682, 836, 875, 1359
HpaII CCGG 7 cut(s) 506, 519, 562, 665, 1217, 1263, 1298
HphI GGTGA 4 cut(s) 168, 968, 1286, 1540
HpyAV CCTTC 4 cut(s) 388, 467, 1482, 1521
HpyCH4III ACNGT 5 cut(s) 601, 655, 1120, 1150, 1473
HpyCH4IV ACGT 2 cut(s) 962, 1209
HpyF10VI GCNNNNNNNGC 2 cut(s) 791, 1063
HpyF3I CTNAG 4 cut(s) 730, 825, 900, 1584
HpySE526I ACGT 2 cut(s) 962, 1209
Hsp92I GRCGYC 1 cut(s) 1209
HspAI GCGC 1 cut(s) 625
Kpn2I TCCGGA 1 cut(s) 1262
Ksp22I TGATCA 3 cut(s) 466, 697, 1333
Kzo9I GATC 8 cut(s) 466, 697, 735, 882, 946, 1093, 1300, 1333
LmnI GCTCC 2 cut(s) 1400, 1486
Lsp1109I GCAGC 2 cut(s) 123, 1078
LweI GCATC 5 cut(s) 301, 522, 991, 1018, 1377
MaeII ACGT 2 cut(s) 962, 1209
MaeIII GTNAC 6 cut(s) 174, 539, 800, 872, 1205, 1499
MalI GATC 8 cut(s) 468, 699, 737, 884, 948, 1095, 1302, 1335
MboI GATC 8 cut(s) 466, 697, 735, 882, 946, 1093, 1300, 1333
MflI RGATCY 3 cut(s) 735, 882, 1300
MhlI GDGCHC 2 cut(s) 708, 1023
MluCI AATT 8 cut(s) 77, 289, 357, 570, 670, 753, 1390, 1565
MlyI GAGTC 4 cut(s) 48, 394, 676, 869
Mph1103I ATGCAT 3 cut(s) 316, 515, 1581
MroI TCCGGA 1 cut(s) 1262
MroXI GAANNNNTTC 2 cut(s) 361, 456
MseI TTAA 3 cut(s) 917, 1278, 1568
MslI CAYNNNNRTG 5 cut(s) 486, 618, 864, 942, 1497
MspI CCGG 7 cut(s) 506, 519, 562, 665, 1217, 1263, 1298
MspR9I CCNGG 7 cut(s) 269, 520, 665, 930, 1217, 1298, 1417
Mva1269I GAATGC 2 cut(s) 1556, 1579
MvaI CCWGG 3 cut(s) 269, 930, 1417
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 2 cut(s) 791, 1063
NciI CCSGG 4 cut(s) 520, 665, 1217, 1298
NdeI CATATG 2 cut(s) 316, 1194
NdeII GATC 8 cut(s) 466, 697, 735, 882, 946, 1093, 1300, 1333
NlaIV GGNNCC 2 cut(s) 1251, 1302
NmuCI GTSAC 4 cut(s) 174, 872, 1205, 1499
NsiI ATGCAT 3 cut(s) 316, 515, 1581
NspI RCATGY 1 cut(s) 1579
NspV TTCGAA 1 cut(s) 365
PaeR7I CTCGAG 1 cut(s) 877
PagI TCATGA 3 cut(s) 82, 700, 1362
PcsI WCGNNNNNNNCGW 2 cut(s) 299, 580
PctI GAATGC 2 cut(s) 1556, 1579
PdmI GAANNNNTTC 2 cut(s) 361, 456
PfeI GAWTC 2 cut(s) 836, 1359
PflMI CCANNNNNTGG 2 cut(s) 71, 132
PfoI TCCNGGA 1 cut(s) 663
PkrI GCNGC 2 cut(s) 113, 1068
PleI GAGTC 4 cut(s) 47, 393, 676, 869
PpsI GAGTC 4 cut(s) 47, 393, 676, 869
PpuMI RGGWCCY 1 cut(s) 1250
PshBI ATTAAT 1 cut(s) 1568
Psp124BI GAGCTC 1 cut(s) 708
Psp5II RGGWCCY 1 cut(s) 1250
Psp6I CCWGG 3 cut(s) 267, 928, 1415
PspGI CCWGG 3 cut(s) 267, 928, 1415
PspN4I GGNNCC 2 cut(s) 1251, 1302
PspPI GGNCC 2 cut(s) 1250, 1456
PspPPI RGGWCCY 1 cut(s) 1250
PspXI VCTCGAGB 1 cut(s) 877
PstI CTGCAG 1 cut(s) 1068
PsuI RGATCY 3 cut(s) 735, 882, 1300
RseI CAYNNNNRTG 5 cut(s) 486, 618, 864, 942, 1497
SacI GAGCTC 1 cut(s) 708
SaqAI TTAA 3 cut(s) 917, 1278, 1568
SatI GCNGC 2 cut(s) 112, 1067
Sau3AI GATC 8 cut(s) 466, 697, 735, 882, 946, 1093, 1300, 1333
Sau96I GGNCC 2 cut(s) 1250, 1456
SchI GAGTC 4 cut(s) 48, 394, 676, 869
ScrFI CCNGG 7 cut(s) 269, 520, 665, 930, 1217, 1298, 1417
SduI GDGCHC 2 cut(s) 708, 1023
SfaNI GCATC 5 cut(s) 301, 522, 991, 1018, 1377
SfcI CTRYAG 1 cut(s) 1064
Sfr274I CTCGAG 1 cut(s) 877
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 2 cut(s) 1250, 1456
SlaI CTCGAG 1 cut(s) 877
SmiMI CAYNNNNRTG 5 cut(s) 486, 618, 864, 942, 1497
SmlI CTYRAG 3 cut(s) 877, 1016, 1424
SmoI CTYRAG 3 cut(s) 877, 1016, 1424
Sse9I AATT 8 cut(s) 77, 289, 357, 570, 670, 753, 1390, 1565
SsiI CCGC 1 cut(s) 1524
SstI GAGCTC 1 cut(s) 708
StyD4I CCNGG 7 cut(s) 267, 518, 663, 928, 1215, 1296, 1415
StyI CCWWGG 1 cut(s) 996
TaaI ACNGT 5 cut(s) 601, 655, 1120, 1150, 1473
TaiI ACGT 2 cut(s) 965, 1212
TaqI TCGA 6 cut(s) 49, 365, 450, 878, 1309, 1346
TasI AATT 8 cut(s) 77, 289, 357, 570, 670, 753, 1390, 1565
TfiI GAWTC 2 cut(s) 836, 1359
Tru1I TTAA 3 cut(s) 917, 1278, 1568
Tru9I TTAA 3 cut(s) 917, 1278, 1568
TscAI CASTG 2 cut(s) 133, 825
TseFI GTSAC 4 cut(s) 174, 872, 1205, 1499
TseI GCWGC 2 cut(s) 111, 1066
Tsp45I GTSAC 4 cut(s) 174, 872, 1205, 1499
TspDTI ATGAA 7 cut(s) 71, 99, 649, 828, 926, 1401, 1575
TspGWI ACGGA 2 cut(s) 162, 1200
TspRI CASTG 2 cut(s) 133, 825
Van91I CCANNNNNTGG 2 cut(s) 71, 132
VpaK11BI GGWCC 2 cut(s) 1250, 1456
VspI ATTAAT 1 cut(s) 1568
XapI RAATTY 4 cut(s) 77, 289, 670, 753
XceI RCATGY 1 cut(s) 1579
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XhoI CTCGAG 1 cut(s) 877
XmnI GAANNNNTTC 2 cut(s) 361, 456
ZraI GACGTC 1 cut(s) 1210
Zsp2I ATGCAT 3 cut(s) 316, 515, 1581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.