Prupe.3G221300_v2.0.a1

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0013240
Physical Location & Seq
Forward (+)
22184822 .. 22187119
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G221300.1

Sequence Viewer

Length: 1428 bp
ATGTTGCTACCTCAATCGCCCTCCAAATCCCAGTCGTCTTCTCGGTTGATAAACCTCGTCATAATCTCATCTTCTGTCTTCACGCTCTTTCTTCTTGTGTCATTCTTCCTTGTATTTACCAGCTCCAAGCTTGCACATATCAGCTACTTATCTCAAGATGTGTACTCCTCGCCCCCAACCTCTCTTGAGCACGTTGTGTTCGGAATTGCTTCGAATCAGCAGTCGTGGTCAGCTAAGAGGAAGGAGTATGTCAGGCTCTGGTGGACGAACCCCATGAGGGGATGCGTGTTTCTTGAAAGCCTTCCACCTGGTCATGAACATCATGCCAATGATACTTCTCTTCCTCCAGTATGTATCTCGGAGGACACTACCCGATTTCGTTACACTTACAAAGGTGGCATGAGGTCTGCTATCCGTGTGGCACGCGTTGTTTCGGAGACCGTGGCACTCAACCATTCGAATGTGCGGTGGTATGTTTTTGGGGACGATGACACGGTTTTCTTTCCGGAGAATTTGGTGAAGACACTTTCTAAGTATGATCATGGGCTGTGGTACTACATTGGGACAAACTCAGAGATCTATGAGCAGAACAGAATTTTTGGATTTCGAATGGCTTATGGTGGTGCTGGTTTCGCTATAAGTTCCCCTTTGGCTAAAGTATTGGCAAAGGTGTTTGATTCATGTATAGAAAGATATCCTCATCTATATGGAAGTGACTCCAGGATCTCCTCTTGCTTGGCAGAGCTTGGCATTGGCTTAACTCAGGAGCCTGGCTTCCATCAGAATGATATTCATGGAGATACATTTGGTCTGTTGGCTGCGCATCCACTAACACCCTTGGTATCTCTGCATCACTTGGATCACATAAACCCTGTCTTCCCCAACATGACAACTATCAAGGCTCTGCAACATTTGTTTAAGGCTGTGAATGCTGATTCTCAGAGGGTTCTACAAAAAACAGTTTGCTATGACAGATGGTTTTCATGGACCATAGTAGTGTCATGGGGTTATGCTGTTCAAATTCATGGGAACCATATCTTTCTGCCGGACGTCCTCCCCGTGCAAGAGACGTTCAGCCAGTGGAAAAAGGGAAGTGTTCTGTCAGGAACTTATACTTTCAACACAAGAGAACATCCCAAGGATCCGTGTCGAAGACCGGTTGTTTTCTTTCTGGACAATGTGTCCTCTGCTGGGGATGGGATCAAGAGCATCTACAAAAAGTCCTACGAAAATTGCACCTCTGACATGGGTTCGCCGAGGAAGCTGGAGGAGGTCAGAGTGTACTCACATAAGCTAGACCTTGATATCAAACAGTTGCAGGCTCCAAGAAGGCATTGTTGTGACATATTACCTTCTACAGGTGGCCAAGTGATGGATATTGGAATCAGAGAATGCAAAGATGAAGAATTAATATACATGCACCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

476

Amino Acids

53.83

Weight (kDa)

7.99

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05280 AT1G05280
fragaria_vesca FvH4_6g43600
malus_domestica MD09G1104300.v1.1 MD17G1092000.v1.1
prunus_persica Prupe.3G221300_v2.0.a1
pyrus_communis pycom09g02750 pycom17g08910
rosa_chinensis RchiOBHm_Chr2g0160191
rosa_laevigata RLG00000021203
rosa_multiflora Rmu_sc0000403.1_g000007
rosa_roxburghii Rroxscaffold_2G00089970
rosa_rugosa Rorug02G0482300
rosa_samantha Rh2AG547700 Rh2BG560900 Rh2CG531500 Rh2DG569600
rosa_wichuraiana Rw2G045290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1053
Acc16I TGCGCA 1 cut(s) 822
AccB7I CCANNNNNTGG 1 cut(s) 1372
AccII CGCG 1 cut(s) 426
AccIII TCCGGA 1 cut(s) 505
AciI CCGC 1 cut(s) 466
AclWI GGATC 5 cut(s) 731, 867, 1136, 1149, 1208
AcoI YGGCCR 1 cut(s) 1363
AcsI RAATTY 3 cut(s) 511, 594, 1020
AcyI GRCGYC 1 cut(s) 1050
AdeI CACNNNGTG 1 cut(s) 196
AfaI GTAC 3 cut(s) 164, 554, 1283
AfiI CCNNNNNNNGG 5 cut(s) 277, 278, 1191, 1358, 1372
AflIII ACRYGT 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 1156
AgsI TTSAA 3 cut(s) 296, 1019, 1120
AhdI GACNNNNNGTC 1 cut(s) 1180
AjnI CCWGG 3 cut(s) 307, 719, 769
AluBI AGCT 7 cut(s) 123, 130, 144, 233, 745, 1264, 1294
AluI AGCT 7 cut(s) 123, 130, 144, 233, 745, 1264, 1294
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 431, 1061
AlwI GGATC 5 cut(s) 731, 867, 1136, 1149, 1208
AlwNI CAGNNNCTG 1 cut(s) 258
Aor13HI TCCGGA 1 cut(s) 505
AoxI GGCC 1 cut(s) 1363
ApeKI GCWGC 1 cut(s) 818
ApoI RAATTY 3 cut(s) 511, 594, 1020
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
AseI ATTAAT 1 cut(s) 1409
AsiGI ACCGGT 1 cut(s) 1156
Asp700I GAANNNNTTC 2 cut(s) 208, 300
AspLEI GCGC 1 cut(s) 823
AspS9I GGNCC 1 cut(s) 987
AsuHPI GGTGA 1 cut(s) 529
AsuII TTCGAA 3 cut(s) 212, 458, 607
AvaII GGWCC 1 cut(s) 987
BalI TGGCCA 1 cut(s) 1365
BamHI GGATCC 1 cut(s) 1141
BbsI GAAGAC 5 cut(s) 30, 70, 527, 868, 1159
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 805
BccI CCATC 4 cut(s) 786, 969, 1190, 1366
BciT130I CCWGG 3 cut(s) 309, 721, 771
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 2 cut(s) 431, 1061
BfaI CTAG 1 cut(s) 1295
BfmI CTRYAG 1 cut(s) 1356
BglII AGATCT 1 cut(s) 576
BisI GCNGC 1 cut(s) 819
BlsI GCNGC 1 cut(s) 820
Bme1390I CCNGG 3 cut(s) 309, 721, 771
Bme18I GGWCC 1 cut(s) 987
BmeRI GACNNNNNGTC 1 cut(s) 1180
BmgT120I GGNCC 1 cut(s) 987
BmiI GGNNCC 4 cut(s) 768, 1031, 1143, 1323
BmrFI CCNGG 3 cut(s) 309, 721, 771
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 4 cut(s) 272, 832, 859, 1218
BmuI ACTGGG 1 cut(s) 25
BpiI GAAGAC 5 cut(s) 30, 70, 527, 868, 1159
BpmI CTGGAG 3 cut(s) 330, 703, 1286
Bpu14I TTCGAA 3 cut(s) 212, 458, 607
BpuEI CTTGAG 2 cut(s) 138, 206
BsaHI GRCGYC 1 cut(s) 1050
BsaI GGTCTC 1 cut(s) 431
BsaJI CCNNGG 4 cut(s) 441, 837, 1137, 1256
BsaWI WCCGGW 2 cut(s) 505, 1156
Bsc4I CCNNNNNNNGG 5 cut(s) 277, 278, 1191, 1358, 1372
Bse118I RCCGGY 1 cut(s) 1156
Bse1I ACTGG 3 cut(s) 31, 347, 1078
BseAI TCCGGA 1 cut(s) 505
BseBI CCWGG 3 cut(s) 309, 721, 771
BseDI CCNNGG 4 cut(s) 441, 837, 1137, 1256
BseGI GGATG 4 cut(s) 287, 823, 1132, 1201
BseLI CCNNNNNNNGG 5 cut(s) 277, 278, 1191, 1358, 1372
BseMII CTCAG 3 cut(s) 585, 776, 953
BseNI ACTGG 3 cut(s) 31, 347, 1078
BseRI GAGGAG 3 cut(s) 157, 718, 1283
BseXI GCAGC 1 cut(s) 805
BseYI CCCAGC 1 cut(s) 1190
Bsh1236I CGCG 1 cut(s) 426
BshFI GGCC 1 cut(s) 1365
BshTI ACCGGT 1 cut(s) 1156
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 3 cut(s) 506, 1046, 1157
BslFI GGGAC 2 cut(s) 497, 577
BslI CCNNNNNNNGG 5 cut(s) 277, 278, 1191, 1358, 1372
BsmAI GTCTC 2 cut(s) 431, 1061
BsmBI CGTCTC 1 cut(s) 1061
BsmFI GGGAC 2 cut(s) 497, 577
BsmI GAATGC 2 cut(s) 934, 1397
BsnI GGCC 1 cut(s) 1365
Bso31I GGTCTC 1 cut(s) 431
Bsp119I TTCGAA 3 cut(s) 212, 458, 607
Bsp1286I GDGCHC 1 cut(s) 192
Bsp13I TCCGGA 1 cut(s) 505
Bsp143I GATC 6 cut(s) 538, 576, 723, 859, 1141, 1200
BspACI CCGC 1 cut(s) 466
BspANI GGCC 1 cut(s) 1365
BspCNI CTCAG 3 cut(s) 584, 775, 952
BspEI TCCGGA 1 cut(s) 505
BspFNI CGCG 1 cut(s) 426
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 4 cut(s) 768, 1031, 1143, 1323
BspPI GGATC 5 cut(s) 731, 867, 1136, 1149, 1208
BspT104I TTCGAA 3 cut(s) 212, 458, 607
BspTNI GGTCTC 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 1156
BsrI ACTGG 3 cut(s) 31, 347, 1078
BssAI RCCGGY 1 cut(s) 1156
BssECI CCNNGG 4 cut(s) 441, 837, 1137, 1256
BssMI GATC 6 cut(s) 538, 576, 723, 859, 1141, 1200
BssNI GRCGYC 1 cut(s) 1050
BssT1I CCWWGG 2 cut(s) 837, 1137
Bst2UI CCWGG 3 cut(s) 309, 721, 771
Bst4CI ACNGT 4 cut(s) 442, 496, 961, 1314
Bst6I CTCTTC 1 cut(s) 345
BstACI GRCGYC 1 cut(s) 1050
BstBI TTCGAA 3 cut(s) 212, 458, 607
BstC8I GCNNGC 3 cut(s) 132, 424, 1320
BstDEI CTNAG 5 cut(s) 234, 531, 571, 762, 939
BstDSI CCRYGG 1 cut(s) 441
BstENI CCTNNNNNAGG 1 cut(s) 1356
BstF5I GGATG 4 cut(s) 287, 823, 1132, 1201
BstFNI CGCG 1 cut(s) 426
BstHHI GCGC 1 cut(s) 823
BstKTI GATC 6 cut(s) 541, 579, 726, 862, 1144, 1203
BstMAI GTCTC 2 cut(s) 431, 1061
BstMBI GATC 6 cut(s) 538, 576, 723, 859, 1141, 1200
BstMWI GCNNNNNNNGC 3 cut(s) 632, 929, 1261
BstNI CCWGG 3 cut(s) 309, 721, 771
BstNSI RCATGY 1 cut(s) 1420
BstSCI CCNGG 3 cut(s) 307, 719, 769
BstSFI CTRYAG 1 cut(s) 1356
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 805
BstV2I GAAGAC 5 cut(s) 30, 70, 527, 868, 1159
BstX2I RGATCY 3 cut(s) 576, 723, 1141
BstYI RGATCY 3 cut(s) 576, 723, 1141
BsuRI GGCC 1 cut(s) 1365
BtgI CCRYGG 1 cut(s) 441
BtsCI GGATG 4 cut(s) 287, 823, 1132, 1201
BtsIMutI CAGTG 1 cut(s) 1085
Cac8I GCNNGC 3 cut(s) 132, 424, 1320
CaiI CAGNNNCTG 1 cut(s) 258
CciI TCATGA 1 cut(s) 313
CfoI GCGC 1 cut(s) 823
Cfr10I RCCGGY 1 cut(s) 1156
Cfr13I GGNCC 1 cut(s) 987
CsiI ACCWGGT 1 cut(s) 307
Csp6I GTAC 3 cut(s) 163, 553, 1282
CspAI ACCGGT 1 cut(s) 1156
CviQI GTAC 3 cut(s) 163, 553, 1282
DdeI CTNAG 5 cut(s) 234, 531, 571, 762, 939
DpnI GATC 6 cut(s) 540, 578, 725, 861, 1143, 1202
DpnII GATC 6 cut(s) 538, 576, 723, 859, 1141, 1200
DraIII CACNNNGTG 1 cut(s) 196
DriI GACNNNNNGTC 1 cut(s) 1180
EaeI YGGCCR 1 cut(s) 1363
Eam1104I CTCTTC 1 cut(s) 345
Eam1105I GACNNNNNGTC 1 cut(s) 1180
EarI CTCTTC 1 cut(s) 345
Eco130I CCWWGG 2 cut(s) 837, 1137
Eco31I GGTCTC 1 cut(s) 431
Eco32I GATATC 2 cut(s) 695, 1306
Eco47I GGWCC 1 cut(s) 987
EcoNI CCTNNNNNAGG 1 cut(s) 1356
EcoRII CCWGG 3 cut(s) 307, 719, 769
EcoRV GATATC 2 cut(s) 695, 1306
EcoT14I CCWWGG 2 cut(s) 837, 1137
ErhI CCWWGG 2 cut(s) 837, 1137
Esp3I CGTCTC 1 cut(s) 1061
FalI AAGNNNNNCTT 2 cut(s) 631, 663
FaqI GGGAC 2 cut(s) 497, 577
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 1 cut(s) 819
FokI GGATG 4 cut(s) 294, 810, 1119, 1208
Fsp4HI GCNGC 1 cut(s) 819
FspBI CTAG 1 cut(s) 1295
FspI TGCGCA 1 cut(s) 822
GlaI GCGC 1 cut(s) 822
GluI GCNGC 1 cut(s) 819
GsaI CCCAGC 1 cut(s) 1194
GsuI CTGGAG 3 cut(s) 330, 703, 1286
HaeIII GGCC 1 cut(s) 1365
HapII CCGG 3 cut(s) 506, 1046, 1157
HhaI GCGC 1 cut(s) 823
Hin1I GRCGYC 1 cut(s) 1050
Hin6I GCGC 1 cut(s) 821
HinP1I GCGC 1 cut(s) 821
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 5 cut(s) 214, 677, 716, 935, 1383
HpaII CCGG 3 cut(s) 506, 1046, 1157
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 3 cut(s) 163, 264, 1282
Hpy188I TCNGA 9 cut(s) 203, 361, 436, 574, 783, 942, 1243, 1277, 1388
Hpy188III TCNNGA 9 cut(s) 155, 185, 293, 314, 506, 764, 1104, 1172, 1204
Hpy8I GTNNAC 3 cut(s) 163, 264, 1282
HpyAV CCTTC 4 cut(s) 235, 311, 1323, 1362
HpyCH4III ACNGT 4 cut(s) 442, 496, 961, 1314
HpyCH4IV ACGT 3 cut(s) 192, 1050, 1070
HpyCH4V TGCA 8 cut(s) 134, 850, 907, 1063, 1236, 1318, 1395, 1420
HpyF10VI GCNNNNNNNGC 3 cut(s) 632, 929, 1261
HpyF3I CTNAG 5 cut(s) 234, 531, 571, 762, 939
HpySE526I ACGT 3 cut(s) 192, 1050, 1070
Hsp92I GRCGYC 1 cut(s) 1050
HspAI GCGC 1 cut(s) 821
Kpn2I TCCGGA 1 cut(s) 505
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 6 cut(s) 538, 576, 723, 859, 1141, 1200
LmnI GCTCC 3 cut(s) 128, 766, 1327
Lsp1109I GCAGC 1 cut(s) 805
LweI GCATC 4 cut(s) 272, 832, 859, 1218
MabI ACCWGGT 1 cut(s) 307
MaeI CTAG 1 cut(s) 1295
MaeII ACGT 3 cut(s) 192, 1050, 1070
MaeIII GTNAC 3 cut(s) 380, 713, 1340
MalI GATC 6 cut(s) 540, 578, 725, 861, 1143, 1202
MboI GATC 6 cut(s) 538, 576, 723, 859, 1141, 1200
MflI RGATCY 3 cut(s) 576, 723, 1141
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 1365
MluCI AATT 6 cut(s) 204, 511, 594, 1020, 1231, 1406
MluI ACGCGT 1 cut(s) 424
MluNI TGGCCA 1 cut(s) 1365
MlyI GAGTC 1 cut(s) 710
Mox20I TGGCCA 1 cut(s) 1365
MroI TCCGGA 1 cut(s) 505
MroXI GAANNNNTTC 2 cut(s) 208, 300
MscI TGGCCA 1 cut(s) 1365
MseI TTAA 3 cut(s) 758, 918, 1409
MslI CAYNNNNRTG 6 cut(s) 327, 459, 705, 783, 995, 1338
Msp20I TGGCCA 1 cut(s) 1365
MspI CCGG 3 cut(s) 506, 1046, 1157
MspR9I CCNGG 3 cut(s) 309, 721, 771
Mva1269I GAATGC 2 cut(s) 934, 1397
MvaI CCWGG 3 cut(s) 309, 721, 771
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 3 cut(s) 632, 929, 1261
NdeII GATC 6 cut(s) 538, 576, 723, 859, 1141, 1200
NlaIV GGNNCC 4 cut(s) 768, 1031, 1143, 1323
NmeAIII GCCGAG 1 cut(s) 1281
NmuCI GTSAC 2 cut(s) 713, 1340
NsbI TGCGCA 1 cut(s) 822
NspI RCATGY 1 cut(s) 1420
NspV TTCGAA 3 cut(s) 212, 458, 607
PagI TCATGA 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 1056
PctI GAATGC 2 cut(s) 934, 1397
PdmI GAANNNNTTC 2 cut(s) 208, 300
PfeI GAWTC 4 cut(s) 214, 677, 935, 1383
PflMI CCANNNNNTGG 1 cut(s) 1372
PfoI TCCNGGA 1 cut(s) 719
PinAI ACCGGT 1 cut(s) 1156
PkrI GCNGC 1 cut(s) 820
PleI GAGTC 1 cut(s) 710
PpsI GAGTC 1 cut(s) 710
PshBI ATTAAT 1 cut(s) 1409
Psp6I CCWGG 3 cut(s) 307, 719, 769
PspFI CCCAGC 1 cut(s) 1190
PspGI CCWGG 3 cut(s) 307, 719, 769
PspN4I GGNNCC 4 cut(s) 768, 1031, 1143, 1323
PspPI GGNCC 1 cut(s) 987
PstNI CAGNNNCTG 1 cut(s) 258
PsuI RGATCY 3 cut(s) 576, 723, 1141
RsaI GTAC 3 cut(s) 164, 554, 1283
RsaNI GTAC 3 cut(s) 163, 553, 1282
RseI CAYNNNNRTG 6 cut(s) 327, 459, 705, 783, 995, 1338
SaqAI TTAA 3 cut(s) 758, 918, 1409
SatI GCNGC 1 cut(s) 819
Sau3AI GATC 6 cut(s) 538, 576, 723, 859, 1141, 1200
Sau96I GGNCC 1 cut(s) 987
SchI GAGTC 1 cut(s) 710
ScrFI CCNGG 3 cut(s) 309, 721, 771
SduI GDGCHC 1 cut(s) 192
SexAI ACCWGGT 1 cut(s) 307
SfaNI GCATC 4 cut(s) 272, 832, 859, 1218
SfcI CTRYAG 1 cut(s) 1356
SfuI TTCGAA 3 cut(s) 212, 458, 607
SinI GGWCC 1 cut(s) 987
SmiMI CAYNNNNRTG 6 cut(s) 327, 459, 705, 783, 995, 1338
SmlI CTYRAG 2 cut(s) 153, 185
SmoI CTYRAG 2 cut(s) 153, 185
Sse9I AATT 6 cut(s) 204, 511, 594, 1020, 1231, 1406
SsiI CCGC 1 cut(s) 466
SspMI CTAG 1 cut(s) 1295
StyD4I CCNGG 3 cut(s) 307, 719, 769
StyI CCWWGG 2 cut(s) 837, 1137
TaaI ACNGT 4 cut(s) 442, 496, 961, 1314
TaiI ACGT 3 cut(s) 195, 1053, 1073
TaqI TCGA 4 cut(s) 212, 458, 607, 1150
TasI AATT 6 cut(s) 204, 511, 594, 1020, 1231, 1406
TatI WGTACW 2 cut(s) 162, 1281
TfiI GAWTC 4 cut(s) 214, 677, 935, 1383
Tru1I TTAA 3 cut(s) 758, 918, 1409
Tru9I TTAA 3 cut(s) 758, 918, 1409
TscAI CASTG 1 cut(s) 1085
TseFI GTSAC 2 cut(s) 713, 1340
TseI GCWGC 1 cut(s) 818
Tsp45I GTSAC 2 cut(s) 713, 1340
TspDTI ATGAA 6 cut(s) 330, 669, 782, 972, 1013, 1416
TspGWI ACGGA 2 cut(s) 404, 1134
TspRI CASTG 1 cut(s) 1085
Van91I CCANNNNNTGG 1 cut(s) 1372
VpaK11BI GGWCC 1 cut(s) 987
VspI ATTAAT 1 cut(s) 1409
XagI CCTNNNNNAGG 1 cut(s) 1356
XapI RAATTY 3 cut(s) 511, 594, 1020
XceI RCATGY 1 cut(s) 1420
XmnI GAANNNNTTC 2 cut(s) 208, 300
XspI CTAG 1 cut(s) 1295
ZraI GACGTC 1 cut(s) 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.