pycom09g02750

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013240
Physical Location & Seq
Reverse (-)
2031952 .. 2033015
1064 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02750.1

Sequence Viewer

Length: 819 bp
ATGGCTTATGGTGGTGCAGGTTTCGCTATAAGTGCCCCGTTGGCAAAAGTACTGGCGAAGGTGTTTGATTCGTGTTTAGAACGATATCCGCATCTCTATGGAAGCGACTCTAGGATTTTCTCTTGCTTGGCAGAGCTCGGCATTGGATTAACACGCGAACCCGGTTTCCATCAGAATGACATTCACGGCGATATGTTTGGTCTGCTGGCTTCGCATCCAGTGACACCTTTGGTATCCCTGCATCACATCGATCACGTAAACCCCATCTTCCCCAACATGACAACTCTCAACGCTCTGCAACATTTGTTTAAAGCTGCCAACGTGGATTCTCAAAGAATGTTACAGAAAACAGTCTGCTATGACAGATGGTTTTCGTGGACTATCATGGTGTCATGGGGTTACGCTGTTCAAGTATACGGAAACCATATTCTTCTGCCGGATGCCCTTCCCGTGCAAGAGACATTCAGCCAGTGGAAAAAGGGAAGTGTTTTGACAGGAGCATATCTTTTAAACACAAGAGAACATCACAAGGATCCGTGTCGAAGGCCTGTTGTTTTCTTTCTGGATAATGTGCCTTCTGGTGCCGGTAGAATCAAGAGCACCTACAAGAAGTCTTACGAAAATTGCACCTACGACATAGCTTCTCCGAGGAGGCTGGAGGAGGTCAAAGTGTACTCGCATAAGCTGGACTTTGATATGAAACAGTTGCAGGCGCCGCGAAGGCAATGTTGCGATGTATTACCTTCTTCGGGCAACAAGGTGATGGAAATCGAAATCAGAGAATGCAAAGAAGAAGAACTAATCCACATGCATCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

273

Amino Acids

30.92

Weight (kDa)

7.66

Isoelectric Point (pI)

56.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF604 PF04646 1 - 244 7.3e-102 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05280 AT1G05280
fragaria_vesca FvH4_6g43600
malus_domestica MD09G1104300.v1.1 MD17G1092000.v1.1
prunus_persica Prupe.3G221300_v2.0.a1
pyrus_communis pycom09g02750 pycom17g08910
rosa_chinensis RchiOBHm_Chr2g0160191
rosa_laevigata RLG00000021203
rosa_multiflora Rmu_sc0000403.1_g000007
rosa_roxburghii Rroxscaffold_2G00089970
rosa_rugosa Rorug02G0482300
rosa_samantha Rh2AG547700 Rh2BG560900 Rh2CG531500 Rh2DG569600
rosa_wichuraiana Rw2G045290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 8
AccB1I GGYRCC 2 cut(s) 581, 712
AccI GTMKAC 1 cut(s) 414
AccII CGCG 2 cut(s) 156, 718
AciI CCGC 2 cut(s) 89, 716
AclWI GGATC 2 cut(s) 527, 540
AcyI GRCGYC 1 cut(s) 713
AfaI GTAC 2 cut(s) 51, 674
AfiI CCNNNNNNNGG 1 cut(s) 749
AgsI TTSAA 1 cut(s) 410
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 4 cut(s) 136, 314, 641, 685
AluI AGCT 4 cut(s) 136, 314, 641, 685
Alw21I GWGCWC 2 cut(s) 138, 602
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 2 cut(s) 527, 540
AoxI GGCC 1 cut(s) 545
ApeKI GCWGC 1 cut(s) 314
AspLEI GCGC 1 cut(s) 715
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 772
BaeGI GKGCMC 1 cut(s) 37
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 2 cut(s) 581, 712
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 2 cut(s) 138, 602
BbvI GCAGC 1 cut(s) 301
BccI CCATC 4 cut(s) 177, 272, 360, 757
BceAI ACGGC 1 cut(s) 202
BciVI GTATCC 1 cut(s) 244
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 1 cut(s) 111
BfoI RGCGCY 1 cut(s) 716
BfuAI ACCTGC 1 cut(s) 8
BfuI GTATCC 1 cut(s) 244
BglI GCCNNNNNGGC 2 cut(s) 41, 721
BisI GCNGC 2 cut(s) 315, 716
BlsI GCNGC 2 cut(s) 316, 717
BmcAI AGTACT 1 cut(s) 51
Bme1390I CCNGG 1 cut(s) 162
BmiI GGNNCC 3 cut(s) 534, 583, 714
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 4 cut(s) 100, 223, 250, 430
BpmI CTGGAG 1 cut(s) 677
BpuMI CCSGG 1 cut(s) 162
Bsa29I ATCGAT 1 cut(s) 249
BsaAI YACGTR 1 cut(s) 256
BsaBI GATNNNNATC 1 cut(s) 767
BsaHI GRCGYC 1 cut(s) 713
BsaJI CCNNGG 1 cut(s) 647
Bsc4I CCNNNNNNNGG 1 cut(s) 749
Bse118I RCCGGY 1 cut(s) 584
Bse1I ACTGG 3 cut(s) 57, 218, 469
Bse3DI GCAATG 1 cut(s) 731
Bse8I GATNNNNATC 1 cut(s) 767
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 647
BseGI GGATG 3 cut(s) 214, 445, 811
BseJI GATNNNNATC 1 cut(s) 767
BseLI CCNNNNNNNGG 1 cut(s) 749
BseMI GCAATG 1 cut(s) 731
BseNI ACTGG 3 cut(s) 57, 218, 469
BseRI GAGGAG 2 cut(s) 664, 674
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 1 cut(s) 301
BsgI GTGCAG 1 cut(s) 36
Bsh1236I CGCG 2 cut(s) 156, 718
BshFI GGCC 1 cut(s) 547
BshNI GGYRCC 2 cut(s) 581, 712
BshVI ATCGAT 1 cut(s) 249
BsiHKAI GWGCWC 2 cut(s) 138, 602
BsiSI CCGG 3 cut(s) 162, 437, 585
BslI CCNNNNNNNGG 1 cut(s) 749
BsmAI GTCTC 1 cut(s) 452
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 1 cut(s) 547
Bsp1286I GDGCHC 3 cut(s) 37, 138, 602
Bsp143I GATC 2 cut(s) 250, 532
BspACI CCGC 2 cut(s) 89, 716
BspANI GGCC 1 cut(s) 547
BspDI ATCGAT 1 cut(s) 249
BspFNI CGCG 2 cut(s) 156, 718
BspLI GGNNCC 3 cut(s) 534, 583, 714
BspMI ACCTGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 527, 540
BspT107I GGYRCC 2 cut(s) 581, 712
BsrDI GCAATG 1 cut(s) 731
BsrFI RCCGGY 1 cut(s) 584
BsrI ACTGG 3 cut(s) 57, 218, 469
BssAI RCCGGY 1 cut(s) 584
BssECI CCNNGG 1 cut(s) 647
BssMI GATC 2 cut(s) 250, 532
BssNAI GTATAC 1 cut(s) 415
BssNI GRCGYC 1 cut(s) 713
Bst1107I GTATAC 1 cut(s) 415
Bst4CI ACNGT 2 cut(s) 352, 705
BstACI GRCGYC 1 cut(s) 713
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 2 cut(s) 207, 711
BstF5I GGATG 3 cut(s) 214, 445, 811
BstFNI CGCG 2 cut(s) 156, 718
BstH2I RGCGCY 1 cut(s) 716
BstHHI GCGC 1 cut(s) 715
BstKTI GATC 2 cut(s) 253, 535
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 2 cut(s) 250, 532
BstMWI GCNNNNNNNGC 6 cut(s) 23, 32, 41, 211, 715, 721
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 1 cut(s) 160
BstSLI GKGCMC 1 cut(s) 37
BstUI CGCG 2 cut(s) 156, 718
BstV1I GCAGC 1 cut(s) 301
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BstZ17I GTATAC 1 cut(s) 415
Bsu15I ATCGAT 1 cut(s) 249
BsuI GTATCC 1 cut(s) 244
BsuRI GGCC 1 cut(s) 547
BsuTUI ATCGAT 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 747
BtsCI GGATG 3 cut(s) 214, 445, 811
BtsIMutI CAGTG 2 cut(s) 225, 476
BveI ACCTGC 1 cut(s) 8
Cac8I GCNNGC 2 cut(s) 207, 711
CfoI GCGC 1 cut(s) 715
Cfr10I RCCGGY 1 cut(s) 584
ClaI ATCGAT 1 cut(s) 249
Csp6I GTAC 2 cut(s) 50, 673
CviAII CATG 4 cut(s) 277, 385, 393, 808
CviJI RGCY 9 cut(s) 5, 136, 209, 314, 468, 547, 641, 655, 685
CviKI_1 RGCY 9 cut(s) 5, 136, 209, 314, 468, 547, 641, 655, 685
CviQI GTAC 2 cut(s) 50, 673
DinI GGCGCC 1 cut(s) 714
DpnI GATC 2 cut(s) 252, 534
DpnII GATC 2 cut(s) 250, 532
DraI TTTAAA 2 cut(s) 310, 510
Ecl136II GAGCTC 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 547
Eco24I GRGCYC 1 cut(s) 138
Eco32I GATATC 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 136
EcoRV GATATC 1 cut(s) 86
EcoT22I ATGCAT 1 cut(s) 813
EcoT38I GRGCYC 1 cut(s) 138
EgeI GGCGCC 1 cut(s) 714
EheI GGCGCC 1 cut(s) 714
FaeI CATG 4 cut(s) 280, 388, 396, 811
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FatI CATG 4 cut(s) 276, 384, 392, 807
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 2 cut(s) 315, 716
FokI GGATG 3 cut(s) 201, 452, 798
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 315, 716
FspBI CTAG 1 cut(s) 111
GlaI GCGC 1 cut(s) 714
GluI GCNGC 2 cut(s) 315, 716
GsuI CTGGAG 1 cut(s) 677
HaeII RGCGCY 1 cut(s) 716
HaeIII GGCC 1 cut(s) 547
HapII CCGG 3 cut(s) 162, 437, 585
HhaI GCGC 1 cut(s) 715
Hin1I GRCGYC 1 cut(s) 713
Hin1II CATG 4 cut(s) 280, 388, 396, 811
Hin6I GCGC 1 cut(s) 713
HinP1I GCGC 1 cut(s) 713
HinfI GANTC 4 cut(s) 68, 107, 326, 591
HpaII CCGG 3 cut(s) 162, 437, 585
HphI GGTGA 1 cut(s) 772
Hpy166II GTNNAC 4 cut(s) 259, 378, 415, 673
Hpy188I TCNGA 3 cut(s) 174, 648, 779
Hpy188III TCNNGA 2 cut(s) 563, 595
Hpy8I GTNNAC 4 cut(s) 259, 378, 415, 673
HpyAV CCTTC 6 cut(s) 52, 455, 537, 585, 714, 753
HpyCH4III ACNGT 2 cut(s) 352, 705
HpyCH4IV ACGT 2 cut(s) 255, 321
HpyCH4V TGCA 8 cut(s) 17, 241, 298, 454, 627, 709, 786, 811
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 32, 41, 211, 715, 721
HpySE526I ACGT 2 cut(s) 255, 321
Hsp92I GRCGYC 1 cut(s) 713
Hsp92II CATG 4 cut(s) 280, 388, 396, 811
HspAI GCGC 1 cut(s) 713
KasI GGCGCC 1 cut(s) 712
Kzo9I GATC 2 cut(s) 250, 532
LmnI GCTCC 1 cut(s) 497
Lsp1109I GCAGC 1 cut(s) 301
LweI GCATC 4 cut(s) 100, 223, 250, 430
MaeI CTAG 1 cut(s) 111
MaeII ACGT 2 cut(s) 255, 321
MaeIII GTNAC 3 cut(s) 220, 339, 398
MalI GATC 2 cut(s) 252, 534
MboI GATC 2 cut(s) 250, 532
MboII GAAGA 5 cut(s) 259, 422, 738, 803, 806
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 3 cut(s) 37, 138, 602
MluCI AATT 1 cut(s) 622
Mly113I GGCGCC 1 cut(s) 713
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 4 cut(s) 642, 645, 652, 655
Mph1103I ATGCAT 1 cut(s) 813
MseI TTAA 3 cut(s) 149, 309, 509
MslI CAYNNNNRTG 2 cut(s) 96, 174
MspI CCGG 3 cut(s) 162, 437, 585
MspR9I CCNGG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 788
MvnI CGCG 2 cut(s) 156, 718
MwoI GCNNNNNNNGC 6 cut(s) 23, 32, 41, 211, 715, 721
NarI GGCGCC 1 cut(s) 713
NciI CCSGG 1 cut(s) 162
NdeII GATC 2 cut(s) 250, 532
NlaIII CATG 4 cut(s) 280, 388, 396, 811
NlaIV GGNNCC 3 cut(s) 534, 583, 714
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 1 cut(s) 220
NsiI ATGCAT 1 cut(s) 813
NspI RCATGY 1 cut(s) 811
PceI AGGCCT 1 cut(s) 547
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 3 cut(s) 68, 326, 591
PkrI GCNGC 2 cut(s) 316, 717
PleI GAGTC 1 cut(s) 101
PluTI GGCGCC 1 cut(s) 716
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 534, 583, 714
PsuI RGATCY 1 cut(s) 532
RsaI GTAC 2 cut(s) 51, 674
RsaNI GTAC 2 cut(s) 50, 673
RseI CAYNNNNRTG 2 cut(s) 96, 174
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 3 cut(s) 149, 309, 509
SatI GCNGC 2 cut(s) 315, 716
Sau3AI GATC 2 cut(s) 250, 532
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 3 cut(s) 37, 138, 602
SfaNI GCATC 4 cut(s) 100, 223, 250, 430
SfoI GGCGCC 1 cut(s) 714
SmiMI CAYNNNNRTG 2 cut(s) 96, 174
Sse9I AATT 1 cut(s) 622
SseBI AGGCCT 1 cut(s) 547
SsiI CCGC 2 cut(s) 89, 716
SspDI GGCGCC 1 cut(s) 712
SspMI CTAG 1 cut(s) 111
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 547
StyD4I CCNGG 1 cut(s) 160
TaaI ACNGT 2 cut(s) 352, 705
TaiI ACGT 2 cut(s) 258, 324
TaqI TCGA 3 cut(s) 249, 541, 771
TasI AATT 1 cut(s) 622
TatI WGTACW 2 cut(s) 49, 672
TauI GCSGC 1 cut(s) 718
TfiI GAWTC 3 cut(s) 68, 326, 591
Tru1I TTAA 3 cut(s) 149, 309, 509
Tru9I TTAA 3 cut(s) 149, 309, 509
TscAI CASTG 2 cut(s) 225, 476
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 314
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 713
TspGWI ACGGA 3 cut(s) 432, 525, 804
TspRI CASTG 2 cut(s) 225, 476
XceI RCATGY 1 cut(s) 811
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 1 cut(s) 111
ZrmI AGTACT 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.