Rorug02G0482300

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013240
Physical Location & Seq
Reverse (-)
61214788 .. 61219682
4895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0482300.1

Sequence Viewer

Length: 1002 bp
ATGGAGGTGACAAAGGTCAAACTCAAGGATGATGATGAGGAATTAGCAGGTGGGATGCTTCCAGGGTATCGGTTTCATCCGACTGATGAAGAACTTGTTGGGTTTTATCTACTGGTTAAGGTGGAGAAGAAAAAGATTAGTACTAGAACTTCTACTAGATTGGATCAACTCATCAGACAGATTGATATCTACAAATATGATCCCTGGGATCTTCCAATTAGCACTGAGGGAGACAAAGAGTGCTACTTCTTCTGCAGAAGAGGGAGAAAATACAGGAACAGCATAAGACCTAACAGAGTCACAGGATCTGGATTTTGGAAAGCCACCGGCATTGACAAACCCATTTATTCAGCTAAGGAACCTCTCGAATGCATCGGACTCAAGAAATCGTTAGTGTACTACCGAGGGAGTGCAGGAAAAGGCACCAAAACCGACTGGATGATGAATGAGTTTCGCCTTCCACCTAATGGGAAAAGTTCTACCAACTTCCTCAATGTCAAAGACAATACACAAGAAGCTGAAGTATGGACACTGTGCAGAATATTCAAAAGAATCCCATCTTATAAGAAATATACACCAAATTGGAAGGAAGAAGCCACATCAACTAAGCAGAATCCTAATACAACTGATTCAAATTCTAAAACATGCAGCTTGGAGTCTGAGAACTGCAGTACTCATGATCCATATTCGAGCCTCGAAGACTCGGTAGTCATTCAACACATTAATGAAAGAAAGCCAGTTGTGGAACAAGTCCATCGTGGTGAACCAAATAATCACTGGTTTAGTGGAGGGCAGTTGGGCTCTCTAGCTGATGAACATCATCATGAGGCTCATTCATTCGCAACATCTTATAATAGTTCACCCTTCATGAGAAATGAGCTCTCTTTAAATCCAAGCGAAACCGATGATTTCTTTACAGACGGAAGCTGCTGGGATGAGTTGAGACCAGTAGTTCAATTGGCTATGGATTCATCCACCAAGTTTATGACTCTGTATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

333

Amino Acids

38.36

Weight (kDa)

6.77

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 21 - 153 7.3e-39 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05280 AT1G05280
fragaria_vesca FvH4_6g43600
malus_domestica MD09G1104300.v1.1 MD17G1092000.v1.1
prunus_persica Prupe.3G221300_v2.0.a1
pyrus_communis pycom09g02750 pycom17g08910
rosa_chinensis RchiOBHm_Chr2g0160191
rosa_laevigata RLG00000021203
rosa_multiflora Rmu_sc0000403.1_g000007
rosa_roxburghii Rroxscaffold_2G00089970
rosa_rugosa Rorug02G0482300
rosa_samantha Rh2AG547700 Rh2BG560900 Rh2CG531500 Rh2DG569600
rosa_wichuraiana Rw2G045290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 564, 852
AarI CACCTGC 1 cut(s) 38
AasI GACNNNNNNGTC 1 cut(s) 707
Acc36I ACCTGC 1 cut(s) 38
AccB1I GGYRCC 1 cut(s) 422
AccB7I CCANNNNNTGG 1 cut(s) 467
AclWI GGATC 5 cut(s) 171, 194, 216, 313, 674
AcsI RAATTY 1 cut(s) 634
AcuI CTGAAG 1 cut(s) 540
AfaI GTAC 3 cut(s) 142, 398, 673
AfiI CCNNNNNNNGG 1 cut(s) 467
AgsI TTSAA 4 cut(s) 547, 633, 716, 956
AjnI CCWGG 2 cut(s) 61, 203
AjuI GAANNNNNNNTTGG 2 cut(s) 81, 113
AluBI AGCT 6 cut(s) 353, 518, 651, 809, 880, 927
AluI AGCT 6 cut(s) 353, 518, 651, 809, 880, 927
Alw21I GWGCWC 1 cut(s) 882
Alw26I GTCTC 2 cut(s) 225, 937
AlwI GGATC 5 cut(s) 171, 194, 216, 313, 674
AlwNI CAGNNNCTG 1 cut(s) 308
ApeKI GCWGC 2 cut(s) 648, 927
ApoI RAATTY 1 cut(s) 634
AseI ATTAAT 1 cut(s) 723
AsuHPI GGTGA 3 cut(s) 19, 773, 852
BanI GGYRCC 1 cut(s) 422
BanII GRGCYC 2 cut(s) 803, 882
BarI GAAGNNNNNNTAC 2 cut(s) 133, 165
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 1 cut(s) 882
BbvI GCAGC 2 cut(s) 660, 914
BccI CCATC 2 cut(s) 565, 762
BciT130I CCWGG 2 cut(s) 63, 205
BcoDI GTCTC 2 cut(s) 225, 937
BfaI CTAG 3 cut(s) 144, 156, 806
BfmI CTRYAG 2 cut(s) 253, 667
BfuAI ACCTGC 1 cut(s) 38
BisI GCNGC 2 cut(s) 649, 928
BlsI GCNGC 2 cut(s) 650, 929
BmcAI AGTACT 2 cut(s) 142, 673
Bme1390I CCNGG 2 cut(s) 63, 205
BmiI GGNNCC 2 cut(s) 360, 424
BmrFI CCNGG 2 cut(s) 63, 205
BmsI GCATC 2 cut(s) 45, 381
BoxI GACNNNNGTC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 705
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 2 cut(s) 8, 365
BsaBI GATNNNNATC 2 cut(s) 185, 816
BsaI GGTCTC 1 cut(s) 937
BsaJI CCNNGG 4 cut(s) 62, 203, 204, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 467
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 5 cut(s) 117, 440, 737, 782, 947
Bse8I GATNNNNATC 2 cut(s) 185, 816
BseBI CCWGG 2 cut(s) 63, 205
BseDI CCNNGG 4 cut(s) 62, 203, 204, 403
BseGI GGATG 6 cut(s) 34, 60, 76, 444, 940, 971
BseJI GATNNNNATC 2 cut(s) 185, 816
BseLI CCNNNNNNNGG 1 cut(s) 467
BseMII CTCAG 2 cut(s) 216, 651
BseNI ACTGG 5 cut(s) 117, 440, 737, 782, 947
BseXI GCAGC 2 cut(s) 660, 914
BseYI CCCAGC 1 cut(s) 930
BsgI GTGCAG 2 cut(s) 432, 556
BshNI GGYRCC 1 cut(s) 422
BsiHKAI GWGCWC 1 cut(s) 882
BsiSI CCGG 1 cut(s) 327
BslI CCNNNNNNNGG 1 cut(s) 467
BsmAI GTCTC 2 cut(s) 225, 937
BsmI GAATGC 1 cut(s) 374
Bso31I GGTCTC 1 cut(s) 937
Bsp1286I GDGCHC 2 cut(s) 803, 882
Bsp143I GATC 5 cut(s) 163, 199, 208, 305, 679
BspCNI CTCAG 2 cut(s) 217, 652
BspHI TCATGA 3 cut(s) 676, 823, 867
BspLI GGNNCC 2 cut(s) 360, 424
BspMAI CTGCAG 2 cut(s) 257, 671
BspMI ACCTGC 1 cut(s) 38
BspPI GGATC 5 cut(s) 171, 194, 216, 313, 674
BspT107I GGYRCC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 937
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 5 cut(s) 117, 440, 737, 782, 947
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 4 cut(s) 62, 203, 204, 403
BssMI GATC 5 cut(s) 163, 199, 208, 305, 679
Bst2UI CCWGG 2 cut(s) 63, 205
Bst4CI ACNGT 1 cut(s) 534
Bst6I CTCTTC 1 cut(s) 253
BstDEI CTNAG 4 cut(s) 225, 354, 606, 660
BstF5I GGATG 6 cut(s) 34, 60, 76, 444, 940, 971
BstKTI GATC 5 cut(s) 166, 202, 211, 308, 682
BstMAI GTCTC 2 cut(s) 225, 937
BstMBI GATC 5 cut(s) 163, 199, 208, 305, 679
BstNI CCWGG 2 cut(s) 63, 205
BstNSI RCATGY 1 cut(s) 648
BstPAI GACNNNNGTC 1 cut(s) 14
BstSCI CCNGG 2 cut(s) 61, 203
BstSFI CTRYAG 2 cut(s) 253, 667
BstV1I GCAGC 2 cut(s) 660, 914
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 2 cut(s) 208, 305
BstYI RGATCY 2 cut(s) 208, 305
BtsCI GGATG 6 cut(s) 34, 60, 76, 444, 940, 971
BtsIMutI CAGTG 3 cut(s) 222, 530, 775
BveI ACCTGC 1 cut(s) 38
CaiI CAGNNNCTG 1 cut(s) 308
CciI TCATGA 3 cut(s) 676, 823, 867
Cfr10I RCCGGY 1 cut(s) 326
Csp6I GTAC 3 cut(s) 141, 397, 672
CspCI CAANNNNNGTGG 2 cut(s) 313, 348
CviAII CATG 4 cut(s) 645, 677, 824, 868
CviQI GTAC 3 cut(s) 141, 397, 672
DdeI CTNAG 4 cut(s) 225, 354, 606, 660
DpnI GATC 5 cut(s) 165, 201, 210, 307, 681
DpnII GATC 5 cut(s) 163, 199, 208, 305, 679
DraI TTTAAA 1 cut(s) 888
DrdI GACNNNNNNGTC 1 cut(s) 707
DseDI GACNNNNNNGTC 1 cut(s) 707
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
Ecl136II GAGCTC 1 cut(s) 880
Eco24I GRGCYC 2 cut(s) 803, 882
Eco31I GGTCTC 1 cut(s) 937
Eco32I GATATC 1 cut(s) 187
Eco53kI GAGCTC 1 cut(s) 880
Eco57I CTGAAG 1 cut(s) 540
EcoICRI GAGCTC 1 cut(s) 880
EcoRII CCWGG 2 cut(s) 61, 203
EcoRV GATATC 1 cut(s) 187
EcoT22I ATGCAT 1 cut(s) 374
EcoT38I GRGCYC 2 cut(s) 803, 882
FaeI CATG 4 cut(s) 648, 680, 827, 871
FatI CATG 4 cut(s) 644, 676, 823, 867
Fnu4HI GCNGC 2 cut(s) 649, 928
FokI GGATG 6 cut(s) 41, 63, 67, 451, 947, 958
FriOI GRGCYC 2 cut(s) 803, 882
Fsp4HI GCNGC 2 cut(s) 649, 928
FspBI CTAG 3 cut(s) 144, 156, 806
GluI GCNGC 2 cut(s) 649, 928
GsaI CCCAGC 1 cut(s) 934
HapII CCGG 1 cut(s) 327
Hin1II CATG 4 cut(s) 648, 680, 827, 871
HinfI GANTC 9 cut(s) 297, 378, 552, 613, 629, 656, 701, 968, 988
HpaII CCGG 1 cut(s) 327
HphI GGTGA 3 cut(s) 19, 773, 852
Hpy166II GTNNAC 3 cut(s) 397, 764, 860
Hpy188I TCNGA 4 cut(s) 81, 176, 377, 661
Hpy188III TCNNGA 6 cut(s) 309, 365, 382, 677, 824, 868
Hpy8I GTNNAC 3 cut(s) 397, 764, 860
HpyAV CCTTC 3 cut(s) 467, 580, 874
HpyCH4III ACNGT 1 cut(s) 534
HpyCH4V TGCA 6 cut(s) 255, 372, 413, 537, 648, 669
HpyF3I CTNAG 4 cut(s) 225, 354, 606, 660
Hsp92II CATG 4 cut(s) 648, 680, 827, 871
Kzo9I GATC 5 cut(s) 163, 199, 208, 305, 679
Lsp1109I GCAGC 2 cut(s) 660, 914
LweI GCATC 2 cut(s) 45, 381
MaeI CTAG 3 cut(s) 144, 156, 806
MaeIII GTNAC 2 cut(s) 7, 298
MalI GATC 5 cut(s) 165, 201, 210, 307, 681
MboI GATC 5 cut(s) 163, 199, 208, 305, 679
MboII GAAGA 7 cut(s) 101, 139, 203, 241, 270, 602, 710
MfeI CAATTG 1 cut(s) 956
MflI RGATCY 2 cut(s) 208, 305
MhlI GDGCHC 2 cut(s) 803, 882
MluCI AATT 5 cut(s) 41, 216, 580, 634, 956
MlyI GAGTC 5 cut(s) 306, 372, 665, 695, 982
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 9 cut(s) 31, 220, 254, 372, 398, 500, 704, 782, 820
Mph1103I ATGCAT 1 cut(s) 374
MseI TTAA 3 cut(s) 117, 723, 887
MslI CAYNNNNRTG 3 cut(s) 723, 759, 822
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 2 cut(s) 63, 205
MunI CAATTG 1 cut(s) 956
Mva1269I GAATGC 1 cut(s) 374
MvaI CCWGG 2 cut(s) 63, 205
NdeII GATC 5 cut(s) 163, 199, 208, 305, 679
NlaIII CATG 4 cut(s) 648, 680, 827, 871
NlaIV GGNNCC 2 cut(s) 360, 424
NmuCI GTSAC 2 cut(s) 7, 298
NsiI ATGCAT 1 cut(s) 374
NspI RCATGY 1 cut(s) 648
PagI TCATGA 3 cut(s) 676, 823, 867
PaqCI CACCTGC 1 cut(s) 38
PasI CCCWGGG 1 cut(s) 204
PctI GAATGC 1 cut(s) 374
PfeI GAWTC 4 cut(s) 552, 613, 629, 968
PflMI CCANNNNNTGG 1 cut(s) 467
PkrI GCNGC 2 cut(s) 650, 929
PleI GAGTC 5 cut(s) 305, 372, 664, 695, 982
PpsI GAGTC 5 cut(s) 305, 372, 664, 695, 982
PshAI GACNNNNGTC 1 cut(s) 14
PshBI ATTAAT 1 cut(s) 723
PsiI TTATAA 2 cut(s) 564, 852
Psp124BI GAGCTC 1 cut(s) 882
Psp6I CCWGG 2 cut(s) 61, 203
PspFI CCCAGC 1 cut(s) 930
PspGI CCWGG 2 cut(s) 61, 203
PspN4I GGNNCC 2 cut(s) 360, 424
PstI CTGCAG 2 cut(s) 257, 671
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 2 cut(s) 208, 305
RsaI GTAC 3 cut(s) 142, 398, 673
RsaNI GTAC 3 cut(s) 141, 397, 672
RseI CAYNNNNRTG 3 cut(s) 723, 759, 822
SacI GAGCTC 1 cut(s) 882
SaqAI TTAA 3 cut(s) 117, 723, 887
SatI GCNGC 2 cut(s) 649, 928
Sau3AI GATC 5 cut(s) 163, 199, 208, 305, 679
ScaI AGTACT 2 cut(s) 142, 673
SchI GAGTC 5 cut(s) 306, 372, 665, 695, 982
ScrFI CCNGG 2 cut(s) 63, 205
SduI GDGCHC 2 cut(s) 803, 882
SfaNI GCATC 2 cut(s) 45, 381
SfcI CTRYAG 2 cut(s) 253, 667
SmiMI CAYNNNNRTG 3 cut(s) 723, 759, 822
SmlI CTYRAG 2 cut(s) 23, 380
SmoI CTYRAG 2 cut(s) 23, 380
Sse9I AATT 5 cut(s) 41, 216, 580, 634, 956
SspI AATATT 1 cut(s) 543
SspMI CTAG 3 cut(s) 144, 156, 806
SstI GAGCTC 1 cut(s) 882
StyD4I CCNGG 2 cut(s) 61, 203
TaaI ACNGT 1 cut(s) 534
TaqI TCGA 3 cut(s) 366, 689, 696
TasI AATT 5 cut(s) 41, 216, 580, 634, 956
TatI WGTACW 3 cut(s) 140, 396, 671
TfiI GAWTC 4 cut(s) 552, 613, 629, 968
Tru1I TTAA 3 cut(s) 117, 723, 887
Tru9I TTAA 3 cut(s) 117, 723, 887
TscAI CASTG 3 cut(s) 229, 537, 782
TseFI GTSAC 2 cut(s) 7, 298
TseI GCWGC 2 cut(s) 648, 927
Tsp45I GTSAC 2 cut(s) 7, 298
TspDTI ATGAA 8 cut(s) 65, 102, 458, 741, 825, 828, 856, 960
TspGWI ACGGA 1 cut(s) 936
TspRI CASTG 3 cut(s) 229, 537, 782
Van91I CCANNNNNTGG 1 cut(s) 467
VspI ATTAAT 1 cut(s) 723
XapI RAATTY 1 cut(s) 634
XceI RCATGY 1 cut(s) 648
XcmI CCANNNNNNNNNTGG 1 cut(s) 774
XspI CTAG 3 cut(s) 144, 156, 806
ZrmI AGTACT 2 cut(s) 142, 673
Zsp2I ATGCAT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.